MypaA.01530.a

EchA1_2

CENTER ID: MypaA.01530.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01530.a.A1.GE29608 Full length( MypaA.01530.a ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01530.a.A1.PS00777 Full length( MypaA.01530.a ) 1 264

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3889
RefSeq: NP_962592.1
UniProt: Q73TR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRHVSTGNEE AGEPEVLVEQ RDRILIITIN RPKAKNAVNA AVANGLAAAV DRLDNEPGLS VGVLTGAGGS FCAGMDLKAF ARGELPIVEG RGMGFTERPP AKPLIAAVEG YALAGGTELA LATDLIVASK DAAFGIPEVK RGLVAGGGGL LRLPQRIPSA IAMELALTGD NLSAERAHAL GMVNVLAEPG AALDEAIALA EKIAANGPLA VAATKRIIVE SRGWSPETMF AEQNKLLAPV FSSNDAKEGA IAFAEKRPPK WTGT
NT Sequence
GTGCGGCACG TGAGCACTGG GAACGAAGAA GCCGGCGAAC CCGAGGTCCT GGTCGAACAA CGGGATCGGA TCCTCATCAT CACGATCAAC CGGCCGAAGG CCAAGAACGC GGTCAACGCC GCGGTGGCCA ACGGACTGGC CGCCGCCGTC GACCGGCTGG ACAACGAGCC CGGTCTGTCG GTCGGCGTCC TGACCGGGGC CGGCGGCTCC TTCTGCGCGG GTATGGATCT GAAGGCCTTC GCCCGCGGCG AGCTGCCCAT CGTCGAGGGC CGCGGCATGG GTTTCACCGA ACGCCCGCCG GCCAAGCCGC TGATCGCGGC GGTCGAGGGT TACGCGCTGG CCGGCGGCAC CGAGCTGGCG CTGGCCACCG ACCTGATCGT CGCCTCCAAG GACGCGGCCT TCGGCATTCC GGAAGTCAAG CGCGGCCTGG TCGCCGGGGG CGGCGGGCTG CTGCGGCTGC CGCAGCGCAT CCCGTCCGCG ATCGCCATGG AGCTGGCGCT GACCGGTGAC AACCTCAGCG CCGAGCGGGC GCACGCGCTC GGCATGGTCA ACGTGCTGGC CGAGCCGGGT GCCGCGCTGG ACGAGGCGAT CGCGCTGGCC GAGAAGATCG CGGCGAACGG GCCGCTGGCG GTGGCCGCCA CCAAGCGGAT CATCGTCGAG TCCCGCGGCT GGAGCCCGGA GACCATGTTC GCCGAGCAGA ACAAGCTGCT GGCCCCCGTG TTCTCCTCCA ACGACGCCAA GGAAGGCGCC ATCGCGTTCG CCGAGAAGCG GCCCCCCAAG TGGACGGGCA CC
Details for MypaA.01530.a.A1.GE29608
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRHVSTGN EEAGEPEVLV EQRDRILIIT INRPKAKNAV NAAVANGLAA AVDRLDNEPG LSVGVLTGAG GSFCAGMDLK AFARGELPIV EGRGMGFTER PPAKPLIAAV EGYALAGGTE LALATDLIVA SKDAAFGIPE VKRGLVAGGG GLLRLPQRIP SAIAMELALT GDNLSAERAH ALGMVNVLAE PGAALDEAIA LAEKIAANGP LAVAATKRII VESRGWSPET MFAEQNKLLA PVFSSNDAKE GAIAFAEKRP PKWTGT
Validated NT Sequence
ttattaggtg cccgtccact tggggggccg cttctcggcg aacgcgatgg cgccttcctt ggcgtcgttg gaggagaaca cgggggccag cagcttgttc tgctcggcga acatggtctc cgggctccag ccgcgggact cgacgatgat ccgcttggtg gcggccaccg ccagcggccc gttcgccgcg atcttctcgg ccagcgcgat cgcctcgtcc agcgcggcac ccggctcggc cagcacgttg accatgccga gcgcgtgcgc ccgctcggcg ctgaggttgt caccggtcag cgccagctcc atggcgatcg cggacgggat gcgctgcggc agccgcagca gcccgccgcc cccggcgacc aggccgcgct tgacttccgg aatgccgaag gccgcgtcct tggaggcgac gatcaggtcg gtggccagcg ccagctcggt gccgccggcc agcgcgtaac cctcgaccgc cgcgatcagc ggcttggccg gcgggcgttc ggtgaaaccc atgccgcggc cctcgacgat gggcagctcg ccgcgggcga aggccttcag atccataccc gcgcagaagg agccgccggc cccggtcagg acgccgaccg acagaccggg ctcgttgtcc agccggtcga cggcggcggc cagtccgttg gccaccgcgg cgttgaccgc gttcttggcc ttcggccggt tgatcgtgat gatgaggatc cgatcccgtt gttcgaccag gacctcgggt tcgccggctt cttcgttccc agtgctcacg tgccgcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRHVSTGN EEAGEPEVLV EQRDRILIIT INRPKAKNAV NAAVANGLAA AVDRLDNEPG LSVGVLTGAG GSFCAGMDLK AFARGELPIV EGRGMGFTER PPAKPLIAAV EGYALAGGTE LALATDLIVA SKDAAFGIPE VKRGLVAGGG GLLRLPQRIP SAIAMELALT GDNLSAERAH ALGMVNVLAE PGAALDEAIA LAEKIAANGP LAVAATKRII VESRGWSPET MFAEQNKLLA PVFSSNDAKE GAIAFAEKRP PKWTGT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcggcac gtgagcactg ggaacgaaga agccggcgaa cccgaggtcc tggtcgaaca acgggatcgg atcctcatca tcacgatcaa ccggccgaag gccaagaacg cggtcaacgc cgcggtggcc aacggactgg ccgccgccgt cgaccggctg gacaacgagc ccggtctgtc ggtcggcgtc ctgaccgggg ccggcggctc cttctgcgcg ggtatggatc tgaaggcctt cgcccgcggc gagctgccca tcgtcgaggg ccgcggcatg ggtttcaccg aacgcccgcc ggccaagccg ctgatcgcgg cggtcgaggg ttacgcgctg gccggcggca ccgagctggc gctggccacc gacctgatcg tcgcctccaa ggacgcggcc ttcggcattc cggaagtcaa gcgcggcctg gtcgccgggg gcggcgggct gctgcggctg ccgcagcgca tcccgtccgc gatcgccatg gagctggcgc tgaccggtga caacctcagc gccgagcggg cgcacgcgct cggcatggtc aacgtgctgg ccgagccggg tgccgcgctg gacgaggcga tcgcgctggc cgagaagatc gcggcgaacg ggccgctggc ggtggccgcc accaagcgga tcatcgtcga gtcccgcggc tggagcccgg agaccatgtt cgccgagcag aacaagctgc tggcccccgt gttctcctcc aacgacgcca aggaaggcgc catcgcgttc gccgagaagc ggccccccaa gtggacgggc accaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MypaA.01530.a.A1.PS00777
PURIFICATION DATe: 9/16/2010
CONCENTRATION: 16.17mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRHVSTGN EEAGEPEVLV EQRDRILIIT INRPKAKNAV NAAVANGLAA AVDRLDNEPG LSVGVLTGAG GSFCAGMDLK AFARGELPIV EGRGMGFTER PPAKPLIAAV EGYALAGGTE LALATDLIVA SKDAAFGIPE VKRGLVAGGG GLLRLPQRIP SAIAMELALT GDNLSAERAH ALGMVNVLAE PGAALDEAIA LAEKIAANGP LAVAATKRII VESRGWSPET MFAEQNKLLA PVFSSNDAKE GAIAFAEKRP PKWTGT
Validated NT Sequence
ttattaggtg cccgtccact tggggggccg cttctcggcg aacgcgatgg cgccttcctt ggcgtcgttg gaggagaaca cgggggccag cagcttgttc tgctcggcga acatggtctc cgggctccag ccgcgggact cgacgatgat ccgcttggtg gcggccaccg ccagcggccc gttcgccgcg atcttctcgg ccagcgcgat cgcctcgtcc agcgcggcac ccggctcggc cagcacgttg accatgccga gcgcgtgcgc ccgctcggcg ctgaggttgt caccggtcag cgccagctcc atggcgatcg cggacgggat gcgctgcggc agccgcagca gcccgccgcc cccggcgacc aggccgcgct tgacttccgg aatgccgaag gccgcgtcct tggaggcgac gatcaggtcg gtggccagcg ccagctcggt gccgccggcc agcgcgtaac cctcgaccgc cgcgatcagc ggcttggccg gcgggcgttc ggtgaaaccc atgccgcggc cctcgacgat gggcagctcg ccgcgggcga aggccttcag atccataccc gcgcagaagg agccgccggc cccggtcagg acgccgaccg acagaccggg ctcgttgtcc agccggtcga cggcggcggc cagtccgttg gccaccgcgg cgttgaccgc gttcttggcc ttcggccggt tgatcgtgat gatgaggatc cgatcccgtt gttcgaccag gacctcgggt tcgccggctt cttcgttccc agtgctcacg tgccgcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRHVSTGN EEAGEPEVLV EQRDRILIIT INRPKAKNAV NAAVANGLAA AVDRLDNEPG LSVGVLTGAG GSFCAGMDLK AFARGELPIV EGRGMGFTER PPAKPLIAAV EGYALAGGTE LALATDLIVA SKDAAFGIPE VKRGLVAGGG GLLRLPQRIP SAIAMELALT GDNLSAERAH ALGMVNVLAE PGAALDEAIA LAEKIAANGP LAVAATKRII VESRGWSPET MFAEQNKLLA PVFSSNDAKE GAIAFAEKRP PKWTGT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGGCAC GTGAGCACTG GGAACGAAGA AGCCGGCGAA CCCGAGGTCC TGGTCGAACA ACGGGATCGG ATCCTCATCA TCACGATCAA CCGGCCGAAG GCCAAGAACG CGGTCAACGC CGCGGTGGCC AACGGACTGG CCGCCGCCGT CGACCGGCTG GACAACGAGC CCGGTCTGTC GGTCGGCGTC CTGACCGGGG CCGGCGGCTC CTTCTGCGCG GGTATGGATC TGAAGGCCTT CGCCCGCGGC GAGCTGCCCA TCGTCGAGGG CCGCGGCATG GGTTTCACCG AACGCCCGCC GGCCAAGCCG CTGATCGCGG CGGTCGAGGG TTACGCGCTG GCCGGCGGCA CCGAGCTGGC GCTGGCCACC GACCTGATCG TCGCCTCCAA GGACGCGGCC TTCGGCATTC CGGAAGTCAA GCGCGGCCTG GTCGCCGGGG GCGGCGGGCT GCTGCGGCTG CCGCAGCGCA TCCCGTCCGC GATCGCCATG GAGCTGGCGC TGACCGGTGA CAACCTCAGC GCCGAGCGGG CGCACGCGCT CGGCATGGTC AACGTGCTGG CCGAGCCGGG TGCCGCGCTG GACGAGGCGA TCGCGCTGGC CGAGAAGATC GCGGCGAACG GGCCGCTGGC GGTGGCCGCC ACCAAGCGGA TCATCGTCGA GTCCCGCGGC TGGAGCCCGG AGACCATGTT CGCCGAGCAG AACAAGCTGC TGGCCCCCGT GTTCTCCTCC AACGACGCCA AGGAAGGCGC CATCGCGTTC GCCGAGAAGC GGCCCCCCAA GTGGACGGGC ACCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA