MyabA.01530.f
Probable enoyl-CoA hydratase/isomerase
| CENTER ID: | MyabA.01530.f |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01530.f.A1.GE30521 | Full length( MyabA.01530.f ) | 1 | 252 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01530.f.A1.PW30398 | Full length( MyabA.01530.f ) | 1 | 252 |
Structures
3TRR
DEPOSITED: 9/9/2011
DETERMINATION: XRay
CLONE: MyabA.01530.f.A1.GE30521
PROTEIN: MyabA.01530.f.A1.PW30398
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.4662 |
| RefSeq: | YP_001705311.1 |
| UniProt: | B1ML12 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADEVLIEQR DRVLLITINR PDARNAVNRA VSQGLAAAAD QLDSSADLSV AIITGAGGNF CAGMDLKAFV SGEAVLSERG LGFTNVPPRK PIIAAVEGFA LAGGTELVLS CDLVVAGRSA KFGIPEVKRG LVAGAGGLLR LPNRIPYQVA MELALTGESF TAEDAAKYGF INRLVDDGQA LDTALELAAK ITANGPLAVA ATKRIIIESA SWAPEEAFAK QGEILMPIFV SEDAKEGAKA FAEKRAPVWQ GK
NT Sequence
ATGGCCGACG AAGTCCTGAT CGAACAGCGT GACCGTGTCC TACTGATCAC CATCAACCGT CCGGACGCGC GTAACGCGGT CAACAGGGCA GTCAGTCAGG GGCTTGCCGC TGCTGCGGAT CAACTGGACA GCTCCGCCGA CCTGTCCGTC GCCATCATTA CCGGTGCGGG CGGAAACTTT TGCGCCGGAA TGGATCTCAA GGCCTTTGTG AGTGGCGAGG CGGTGTTGTC CGAGCGTGGT CTGGGCTTTA CCAATGTGCC GCCACGCAAG CCGATCATCG CCGCGGTGGA GGGTTTCGCG CTGGCCGGAG GCACGGAGCT GGTGCTGTCC TGCGACCTGG TGGTCGCGGG CCGCAGCGCG AAGTTCGGCA TTCCGGAGGT CAAGCGTGGG CTGGTGGCCG GTGCCGGCGG CCTGCTGCGC CTGCCGAACC GGATCCCGTA TCAGGTCGCC ATGGAGCTGG CGCTCACCGG AGAGTCCTTC ACCGCGGAGG ACGCTGCCAA GTACGGGTTT ATCAACCGGC TTGTGGACGA CGGGCAGGCA CTGGACACCG CACTCGAGCT GGCCGCCAAG ATCACGGCGA ACGGACCGCT GGCCGTAGCG GCCACCAAGC GCATCATCAT CGAGTCGGCC AGCTGGGCTC CCGAGGAAGC CTTCGCTAAG CAGGGCGAGA TCCTCATGCC GATCTTTGTG TCCGAGGACG CCAAGGAAGG CGCCAAGGCG TTTGCGGAGA AGCGCGCGCC CGTCTGGCAG GGCAAG
Details for MyabA.01530.f.A1.GE30521
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 9/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADEVLIEQ RDRVLLITIN RPDARNAVNR AVSQGLAAAA DQLDSSADLS VAIITGAGGN FCAGMDLKAF VSGEAVLSER GLGFTNVPPR KPIIAAVEGF ALAGGTELVL SCDLVVAGRS AKFGIPEVKR GLVAGAGGLL RLPNRIPYQV AMELALTGES FTAEDAAKYG FINRLVDDGQ ALDTALELAA KITANGPLAV AATKRIIIES ASWAPEEAFA KQGEILMPIF VSEDAKEGAK AFAEKRAPVW QGK
Validated NT Sequence
ttattacttg ccctgccaga cgggcgcgcg cttctccgca aacgccttgg cgccttcctt ggcgtcctcg gacacaaaga tcggcatgag gatctcgccc tgcttagcga aggcttcctc gggagcccag ctggccgact cgatgatgat gcgcttggtg gccgctacgg ccagcggtcc gttcgccgtg atcttggcgg ccagctcgag tgcggtgtcc agtgcctgcc cgtcgtccac aagccggttg ataaacccgt acttggcagc gtcctccgcg gtgaaggact ctccggtgag cgccagctcc atggcgacct gatacgggat ccggttcggc aggcgcagca ggccgccggc accggccacc agcccacgct tgacctccgg aatgccgaac ttcgcgctgc ggcccgcgac caccaggtcg caggacagca ccagctccgt gcctccggcc agcgcgaaac cctccaccgc ggcgatgatc ggcttgcgtg gcggcacatt ggtaaagccc agaccacgct cggacaacac cgcctcgcca ctcacaaagg ccttgagatc cattccggcg caaaagtttc cgcccgcacc ggtaatgatg gcgacggaca ggtcggcgga gctgtccagt tgatccgcag cagcggcaag cccctgactg actgccctgt tgaccgcgtt acgcgcgtcc ggacggttga tggtgatcag taggacacgg tcacgctgtt cgatcaggac ttcgtcggcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADEVLIEQ RDRVLLITIN RPDARNAVNR AVSQGLAAAA DQLDSSADLS VAIITGAGGN FCAGMDLKAF VSGEAVLSER GLGFTNVPPR KPIIAAVEGF ALAGGTELVL SCDLVVAGRS AKFGIPEVKR GLVAGAGGLL RLPNRIPYQV AMELALTGES FTAEDAAKYG FINRLVDDGQ ALDTALELAA KITANGPLAV AATKRIIIES ASWAPEEAFA KQGEILMPIF VSEDAKEGAK AFAEKRAPVW QGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgacgaa gtcctgatcg aacagcgtga ccgtgtccta ctgatcacca tcaaccgtcc ggacgcgcgt aacgcggtca acagggcagt cagtcagggg cttgccgctg ctgcggatca actggacagc tccgccgacc tgtccgtcgc catcattacc ggtgcgggcg gaaacttttg cgccggaatg gatctcaagg cctttgtgag tggcgaggcg gtgttgtccg agcgtggtct gggctttacc aatgtgccgc cacgcaagcc gatcatcgcc gcggtggagg gtttcgcgct ggccggaggc acggagctgg tgctgtcctg cgacctggtg gtcgcgggcc gcagcgcgaa gttcggcatt ccggaggtca agcgtgggct ggtggccggt gccggcggcc tgctgcgcct gccgaaccgg atcccgtatc aggtcgccat ggagctggcg ctcaccggag agtccttcac cgcggaggac gctgccaagt acgggtttat caaccggctt gtggacgacg ggcaggcact ggacaccgca ctcgagctgg ccgccaagat cacggcgaac ggaccgctgg ccgtagcggc caccaagcgc atcatcatcg agtcggccag ctgggctccc gaggaagcct tcgctaagca gggcgagatc ctcatgccga tctttgtgtc cgaggacgcc aaggaaggcg ccaaggcgtt tgcggagaag cgcgcgcccg tctggcaggg caagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.01530.f.A1.PW30398
| PURIFICATION DATe: | 12/6/2010 |
| CONCENTRATION: | 27.53mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADEVLIEQ RDRVLLITIN RPDARNAVNR AVSQGLAAAA DQLDSSADLS VAIITGAGGN FCAGMDLKAF VSGEAVLSER GLGFTNVPPR KPIIAAVEGF ALAGGTELVL SCDLVVAGRS AKFGIPEVKR GLVAGAGGLL RLPNRIPYQV AMELALTGES FTAEDAAKYG FINRLVDDGQ ALDTALELAA KITANGPLAV AATKRIIIES ASWAPEEAFA KQGEILMPIF VSEDAKEGAK AFAEKRAPVW QGK
Validated NT Sequence
ttattacttg ccctgccaga cgggcgcgcg cttctccgca aacgccttgg cgccttcctt ggcgtcctcg gacacaaaga tcggcatgag gatctcgccc tgcttagcga aggcttcctc gggagcccag ctggccgact cgatgatgat gcgcttggtg gccgctacgg ccagcggtcc gttcgccgtg atcttggcgg ccagctcgag tgcggtgtcc agtgcctgcc cgtcgtccac aagccggttg ataaacccgt acttggcagc gtcctccgcg gtgaaggact ctccggtgag cgccagctcc atggcgacct gatacgggat ccggttcggc aggcgcagca ggccgccggc accggccacc agcccacgct tgacctccgg aatgccgaac ttcgcgctgc ggcccgcgac caccaggtcg caggacagca ccagctccgt gcctccggcc agcgcgaaac cctccaccgc ggcgatgatc ggcttgcgtg gcggcacatt ggtaaagccc agaccacgct cggacaacac cgcctcgcca ctcacaaagg ccttgagatc cattccggcg caaaagtttc cgcccgcacc ggtaatgatg gcgacggaca ggtcggcgga gctgtccagt tgatccgcag cagcggcaag cccctgactg actgccctgt tgaccgcgtt acgcgcgtcc ggacggttga tggtgatcag taggacacgg tcacgctgtt cgatcaggac ttcgtcggcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADEVLIEQ RDRVLLITIN RPDARNAVNR AVSQGLAAAA DQLDSSADLS VAIITGAGGN FCAGMDLKAF VSGEAVLSER GLGFTNVPPR KPIIAAVEGF ALAGGTELVL SCDLVVAGRS AKFGIPEVKR GLVAGAGGLL RLPNRIPYQV AMELALTGES FTAEDAAKYG FINRLVDDGQ ALDTALELAA KITANGPLAV AATKRIIIES ASWAPEEAFA KQGEILMPIF VSEDAKEGAK AFAEKRAPVW QGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGACGAA GTCCTGATCG AACAGCGTGA CCGTGTCCTA CTGATCACCA TCAACCGTCC GGACGCGCGT AACGCGGTCA ACAGGGCAGT CAGTCAGGGG CTTGCCGCTG CTGCGGATCA ACTGGACAGC TCCGCCGACC TGTCCGTCGC CATCATTACC GGTGCGGGCG GAAACTTTTG CGCCGGAATG GATCTCAAGG CCTTTGTGAG TGGCGAGGCG GTGTTGTCCG AGCGTGGTCT GGGCTTTACC AATGTGCCGC CACGCAAGCC GATCATCGCC GCGGTGGAGG GTTTCGCGCT GGCCGGAGGC ACGGAGCTGG TGCTGTCCTG CGACCTGGTG GTCGCGGGCC GCAGCGCGAA GTTCGGCATT CCGGAGGTCA AGCGTGGGCT GGTGGCCGGT GCCGGCGGCC TGCTGCGCCT GCCGAACCGG ATCCCGTATC AGGTCGCCAT GGAGCTGGCG CTCACCGGAG AGTCCTTCAC CGCGGAGGAC GCTGCCAAGT ACGGGTTTAT CAACCGGCTT GTGGACGACG GGCAGGCACT GGACACCGCA CTCGAGCTGG CCGCCAAGAT CACGGCGAAC GGACCGCTGG CCGTAGCGGC CACCAAGCGC ATCATCATCG AGTCGGCCAG CTGGGCTCCC GAGGAAGCCT TCGCTAAGCA GGGCGAGATC CTCATGCCGA TCTTTGTGTC CGAGGACGCC AAGGAAGGCG CCAAGGCGTT TGCGGAGAAG CGCGCGCCCG TCTGGCAGGG CAAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA