MymaA.01530.f
Enoyl-CoA hydratase EchA1
| CENTER ID: | MymaA.01530.f |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01530.f.A1.GE30189 | Full length( MymaA.01530.f ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01530.f.A1.PS00846 | Full length( MymaA.01530.f ) | 1 | 260 |
Structures
3QXI
DEPOSITED: 3/1/2011
DETERMINATION: XRay
CLONE: MymaA.01530.f.A1.GE30189
PROTEIN: MymaA.01530.f.A1.PS00846
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_169926 |
| PATRIC ID: | fig|216594.6.peg.507 |
| RefSeq: | YP_001848785.1 |
| UniProt: | B2HMZ1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSELNGDTEP EVLVEQRDRI LIITINRPKA KNSVNAAVSR ALADAMDRLD ADAGLSVGIL TGAGGSFCAG MDLKAFARGE NVVVEGRGLG FTERPPAKPL IAAVEGYALA GGTELALATD LIVAARDSAF GIPEVKRGLV AGGGGLLRLP ERIPYAIAME LALTGDNLSA ERAHALGMVN VLAEPGAALD AAIALAEKIT ANGPLAVAAT KRIITESRGW SLDTRFAQQM KILFPIFTSN DAKEGAIAFA EKRPPRWTGT
NT Sequence
GTGAGTGAAC TAAACGGCGA CACCGAACCC GAAGTCCTGG TCGAACAGCG GGACCGAATC CTGATCATCA CGATCAATCG GCCCAAGGCC AAGAACTCGG TCAACGCCGC GGTGAGCCGG GCCTTGGCCG ATGCGATGGA TCGCCTCGAT GCTGACGCCG GCCTCTCGGT GGGGATCCTT ACCGGAGCGG GTGGCTCGTT CTGTGCCGGC ATGGACCTCA AGGCGTTTGC CCGCGGCGAG AACGTCGTGG TCGAGGGCCG GGGGCTGGGG TTCACCGAGC GCCCGCCGGC CAAACCGCTG ATCGCGGCGG TCGAAGGATA CGCCTTGGCC GGCGGCACCG AGCTGGCCTT GGCCACCGAT CTGATTGTGG CGGCCAGGGA TTCGGCGTTC GGAATACCGG AGGTGAAACG GGGTCTGGTG GCCGGTGGCG GGGGATTGCT GCGGCTGCCC GAGCGCATTC CCTACGCGAT CGCGATGGAA CTGGCGCTCA CCGGTGACAA CCTGTCCGCC GAACGCGCCC ACGCACTCGG CATGGTCAAC GTGCTGGCCG AACCCGGGGC AGCCCTGGAT GCGGCGATCG CGTTGGCGGA GAAGATCACT GCCAACGGTC CGCTCGCGGT GGCCGCCACC AAACGGATCA TCACCGAATC CCGCGGCTGG AGTCTGGACA CCAGATTCGC CCAGCAGATG AAGATCCTGT TCCCGATTTT CACCTCCAAT GACGCCAAGG AAGGTGCGAT CGCGTTCGCG GAGAAACGTC CGCCCCGCTG GACCGGTACC
Details for MymaA.01530.f.A1.GE30189
| HARVESTED ON: | 8/15/2010 |
| SEQUENCED ON: | 8/25/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSELNGDT EPEVLVEQRD RILIITINRP KAKNSVNAAV SRALADAMDR LDADAGLSVG ILTGAGGSFC AGMDLKAFAR GENVVVEGRG LGFTERPPAK PLIAAVEGYA LAGGTELALA TDLIVAARDS AFGIPEVKRG LVAGGGGLLR LPERIPYAIA MELALTGDNL SAERAHALGM VNVLAEPGAA LDAAIALAEK ITANGPLAVA ATKRIITESR GWSLDTRFAQ QMKILFPIFT SNDAKEGAIA FAEKRPPRWT GT
Validated NT Sequence
ttattaggta ccggtccagc ggggcggacg tttctccgcg aacgcgatcg caccttcctt ggcgtcattg gaggtgaaaa tcgggaacag gatcttcatc tgctgggcga atctggtgtc cagactccag ccgcgggatt cggtgatgat ccgtttggtg gcggccaccg cgagcggacc gttggcagtg atcttctccg ccaacgcgat cgccgcatcc agggctgccc cgggttcggc cagcacgttg accatgccga gtgcgtgggc gcgttcggcg gacaggttgt caccggtgag cgccagttcc atcgcgatcg cgtagggaat gcgctcgggc agccgcagca atcccccgcc accggccacc agaccccgtt tcacctccgg tattccgaac gccgaatccc tggccgccac aatcagatcg gtggccaagg ccagctcggt gccgccggcc aaggcgtatc cttcgaccgc cgcgatcagc ggtttggccg gcgggcgctc ggtgaacccc agcccccggc cctcgaccac gacgttctcg ccgcgggcaa acgccttgag gtccatgccg gcacagaacg agccacccgc tccggtaagg atccccaccg agaggccggc gtcagcatcg aggcgatcca tcgcatcggc caaggcccgg ctcaccgcgg cgttgaccga gttcttggcc ttgggccgat tgatcgtgat gatcaggatt cggtcccgct gttcgaccag gacttcgggt tcggtgtcgc cgtttagttc actcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSELNGDT EPEVLVEQRD RILIITINRP KAKNSVNAAV SRALADAMDR LDADAGLSVG ILTGAGGSFC AGMDLKAFAR GENVVVEGRG LGFTERPPAK PLIAAVEGYA LAGGTELALA TDLIVAARDS AFGIPEVKRG LVAGGGGLLR LPERIPYAIA MELALTGDNL SAERAHALGM VNVLAEPGAA LDAAIALAEK ITANGPLAVA ATKRIITESR GWSLDTRFAQ QMKILFPIFT SNDAKEGAIA FAEKRPPRWT GT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgaa ctaaacggcg acaccgaacc cgaagtcctg gtcgaacagc gggaccgaat cctgatcatc acgatcaatc ggcccaaggc caagaactcg gtcaacgccg cggtgagccg ggccttggcc gatgcgatgg atcgcctcga tgctgacgcc ggcctctcgg tggggatcct taccggagcg ggtggctcgt tctgtgccgg catggacctc aaggcgtttg cccgcggcga gaacgtcgtg gtcgagggcc gggggctggg gttcaccgag cgcccgccgg ccaaaccgct gatcgcggcg gtcgaaggat acgccttggc cggcggcacc gagctggcct tggccaccga tctgattgtg gcggccaggg attcggcgtt cggaataccg gaggtgaaac ggggtctggt ggccggtggc gggggattgc tgcggctgcc cgagcgcatt ccctacgcga tcgcgatgga actggcgctc accggtgaca acctgtccgc cgaacgcgcc cacgcactcg gcatggtcaa cgtgctggcc gaacccgggg cagccctgga tgcggcgatc gcgttggcgg agaagatcac tgccaacggt ccgctcgcgg tggccgccac caaacggatc atcaccgaat cccgcggctg gagtctggac accagattcg cccagcagat gaagatcctg ttcccgattt tcacctccaa tgacgccaag gaaggtgcga tcgcgttcgc ggagaaacgt ccgccccgct ggaccggtac caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.01530.f.A1.PS00846
| PURIFICATION DATe: | 11/4/2010 |
| CONCENTRATION: | 33.15mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSELNGDT EPEVLVEQRD RILIITINRP KAKNSVNAAV SRALADAMDR LDADAGLSVG ILTGAGGSFC AGMDLKAFAR GENVVVEGRG LGFTERPPAK PLIAAVEGYA LAGGTELALA TDLIVAARDS AFGIPEVKRG LVAGGGGLLR LPERIPYAIA MELALTGDNL SAERAHALGM VNVLAEPGAA LDAAIALAEK ITANGPLAVA ATKRIITESR GWSLDTRFAQ QMKILFPIFT SNDAKEGAIA FAEKRPPRWT GT
Validated NT Sequence
ttattaggta ccggtccagc ggggcggacg tttctccgcg aacgcgatcg caccttcctt ggcgtcattg gaggtgaaaa tcgggaacag gatcttcatc tgctgggcga atctggtgtc cagactccag ccgcgggatt cggtgatgat ccgtttggtg gcggccaccg cgagcggacc gttggcagtg atcttctccg ccaacgcgat cgccgcatcc agggctgccc cgggttcggc cagcacgttg accatgccga gtgcgtgggc gcgttcggcg gacaggttgt caccggtgag cgccagttcc atcgcgatcg cgtagggaat gcgctcgggc agccgcagca atcccccgcc accggccacc agaccccgtt tcacctccgg tattccgaac gccgaatccc tggccgccac aatcagatcg gtggccaagg ccagctcggt gccgccggcc aaggcgtatc cttcgaccgc cgcgatcagc ggtttggccg gcgggcgctc ggtgaacccc agcccccggc cctcgaccac gacgttctcg ccgcgggcaa acgccttgag gtccatgccg gcacagaacg agccacccgc tccggtaagg atccccaccg agaggccggc gtcagcatcg aggcgatcca tcgcatcggc caaggcccgg ctcaccgcgg cgttgaccga gttcttggcc ttgggccgat tgatcgtgat gatcaggatt cggtcccgct gttcgaccag gacttcgggt tcggtgtcgc cgtttagttc actcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSELNGDT EPEVLVEQRD RILIITINRP KAKNSVNAAV SRALADAMDR LDADAGLSVG ILTGAGGSFC AGMDLKAFAR GENVVVEGRG LGFTERPPAK PLIAAVEGYA LAGGTELALA TDLIVAARDS AFGIPEVKRG LVAGGGGLLR LPERIPYAIA MELALTGDNL SAERAHALGM VNVLAEPGAA LDAAIALAEK ITANGPLAVA ATKRIITESR GWSLDTRFAQ QMKILFPIFT SNDAKEGAIA FAEKRPPRWT GT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTGAA CTAAACGGCG ACACCGAACC CGAAGTCCTG GTCGAACAGC GGGACCGAAT CCTGATCATC ACGATCAATC GGCCCAAGGC CAAGAACTCG GTCAACGCCG CGGTGAGCCG GGCCTTGGCC GATGCGATGG ATCGCCTCGA TGCTGACGCC GGCCTCTCGG TGGGGATCCT TACCGGAGCG GGTGGCTCGT TCTGTGCCGG CATGGACCTC AAGGCGTTTG CCCGCGGCGA GAACGTCGTG GTCGAGGGCC GGGGGCTGGG GTTCACCGAG CGCCCGCCGG CCAAACCGCT GATCGCGGCG GTCGAAGGAT ACGCCTTGGC CGGCGGCACC GAGCTGGCCT TGGCCACCGA TCTGATTGTG GCGGCCAGGG ATTCGGCGTT CGGAATACCG GAGGTGAAAC GGGGTCTGGT GGCCGGTGGC GGGGGATTGC TGCGGCTGCC CGAGCGCATT CCCTACGCGA TCGCGATGGA ACTGGCGCTC ACCGGTGACA ACCTGTCCGC CGAACGCGCC CACGCACTCG GCATGGTCAA CGTGCTGGCC GAACCCGGGG CAGCCCTGGA TGCGGCGATC GCGTTGGCGG AGAAGATCAC TGCCAACGGT CCGCTCGCGG TGGCCGCCAC CAAACGGATC ATCACCGAAT CCCGCGGCTG GAGTCTGGAC ACCAGATTCG CCCAGCAGAT GAAGATCCTG TTCCCGATTT TCACCTCCAA TGACGCCAAG GAAGGTGCGA TCGCGTTCGC GGAGAAACGT CCGCCCCGCT GGACCGGTAC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA