MyulA.00629.c

O-succinylbenzoic acid-CoA ligase, MenE (Rv0542c ortholog)

CENTER ID: MyulA.00629.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00629.c.B1.GE37667 full length 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.816
RefSeq: YP_904777.1
UniProt: A0PLT7

Orthologues for Rv0542c

Species % Identity % Coverage Clones Proteins Structures
marinum 79 100 1 0 0
ulcerans 79 100 1 0 0
paratuberculosis 78 100 0 0 0
leprae 78 99 1 0 0
avium 77 100 0 0 0
fortuitum 68 95 1 0 0
smegmatis 68 95 0 0 0
thermoresistibile 65 98 1 0 0
abscessus 61 99 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLPALERIL AGRAPATVAV GSRPDPTMVS ALRVGDQIDD DVALVVTTSG TTGAPKGALL TAAALIASAS ATHHRLGGAG NWLLAVPAIH IAGLQVLVRS ALAGTVPVEL DVSAGFDITE LPRAVDKLGS GRRYTSLVAA QLAKVLGDPA ATAALAELDA VLIGGGPAPR PVLDAGAAAG IRIVRTYGMS ETCGGCVYDG VALDGVQLRV AADGRIMIGG ATLAKGYRNP VQPDPFAEPG WFRTDDLGAL DDSGVLSVLG RADDAISTGG LTVLPQLVES ALATHAAVSD CAVFGLDDDR LGQRVAAAIV VRAHCPTPTL DQLRAHVAGT LAITAAPREL HVVDELPRRG IGKIDRRALV RRFAAATDQ
NT Sequence
gtgctgttgc ccgccctgga gcggattctt gccggccggg ctccggcaac ggtcgcggtg gggtcaagac ctgaccccac gatggtgagc gcgttgcggg tcggcgacca gatcgatgac gatgtggcgc tggtcgtcac gacgtcggga accaccgggg cgcccaaggg ggcgctcctg accgcggccg cgttgatcgc cagcgcgtcg gcgacccatc accgcctcgg cggagccggc aactggctac tggccgtgcc cgcgattcac atcgccggcc tgcaggtcct ggtgcgcagc gcgctcgccg gtaccgtccc ggtcgaactc gatgtgtccg ccgggttcga catcaccgaa ctgccccgcg ctgtcgacaa gttgggttcc ggccggcgat acacctcgct ggtcgccgct cagctggcca aggtgctggg cgaccccgcc gccaccgccg ccctggccga actggacgcg gtgctgatcg ggggtggacc ggctccgcgt ccggtgctcg acgccggtgc cgccgccggc atccggatcg ttcgcaccta cgggatgagc gaaacctgcg gaggctgcgt gtacgacggt gttgccctcg acggcgtgca actgcgcgtg gccgcggacg gccgcatcat gatcggcggc gcaacgctcg ccaaggggta ccgcaacccg gtacaacccg acccgttcgc cgagcccggc tggtttcgta ccgacgacct cggggcgctc gatgattcgg gcgtgctcag cgtgctgggc cgcgccgacg acgcgatcag cacgggcggg ctcaccgtgc tgccgcagct ggtggaatcc gcgctggcca cccacgccgc tgtgagcgac tgcgcggtgt tcggactcga cgatgaccgg ctgggtcagc gggtcgccgc ggcgatcgtg gtccgcgccc actgccccac cccaacgctg gatcaacttc gggcacacgt cgccggcacg ctggccatca ccgccgcacc ccgagaactg cacgtcgtcg acgagctgcc gcggcgcggc atcggcaaga tcgatcggcg ggcgttggtg cgtcgattcg ccgccgcaac cgaccaatag
Details for MyulA.00629.c.B1.GE37667
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 39kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMV LLPALERILA GRAPATVAVG SRPDPTMVSA LRVGDQIDDD VALVVTTSGT TGAPKGALLT AAALIASASA THHRLGGAGN WLLAVPAIHI AGLQVLVRSA LAGTVPVELD VSAGFDITEL PRAVDKLGSG RRYTSLVAAQ LAKVLGDPAA TAALAELDAV LIGGGPAPRP VLDAGAAAGI RIVRTYGMSE TCGGCVYDGV ALDGVQLRVA ADGRIMIGGA TLAKGYRNPV QPDPFAEPGW FRTDDLGALD DSGVLSVLGR ADDAISTGGL TVLPQLVGIR AXHPRRCERL RGVRTRR
Validated NT Sequence
cnnnnnnnnn tnnnctnnaa tnttttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatggt gctgttgccc gccctggagc ggattcttgc cggccgggct ccggcaacgg tcgcggtggg gtcaagacct gaccccacga tggtgagcgc gttgcgggtc ggcgaccaga tcgatgacga tgtggcgctg gtcgtcacga cgtcgggaac caccggggcg cccaaggggg cgctcctgac cgcggccgcg ttgatcgcca gcgcgtcggc gacccatcac cgcctcggcg gagccggcaa ctggctactg gccgtgcccg cgattcacat cgccggcctg caggtcctgg tgcgcagcgc gctcgccggt accgtcccgg tcgaactcga tgtgtccgcc gggttcgaca tcaccgaact gccccgcgct gtcgacaagt tgggttccgg ccggcgatac acctcgctgg tcgccgctca gctggccaag gtgctgggcg accccgccgc caccgccgcc ctggccgaac tggacgcggt gctgatcggg ggtggaccgg ctccgcgtcc ggtgctcgac gccggtgccg ccgccggcat ccggatcgtt cgcacctacg ggatgagcga aacctgcgga ggctgcgtgt acgacggtgt tgccctcgac ggcgtgcaac tgcgcgtggc cgcggacggc cgcatcatga tcggcggcgc aacgctcgcc aaggggtacc gcaacccggt acaacccgac ccgttcgccg agcccggctg gtttcgtacc gacgacctcg gggcgctcga tgattcgggc gtgctcagcg tgctgggccg cgccgacgac gcgatcagca cgggcgggct caccgtgctg ccgcagctgg tgggaatccg cgctgnccac ccacgccgct gtgagcgact gcgcggtgtt cggactcgac gatgaccggc tgggtcagcg ggtcgccgcg gcgatcgtgn ncgcgcccac tgcccacccc aacgctggat caacttcggg cacacgtcgc cggcacgctg gccatcaccg ccgcnncccg agaactgcac gttcgtcnan nnctnnnnng cccgnatnng cagatcgatc gnnggnnttg gtgnnnnnnt tcgccnnccn caacnaacan nanntganna anaanngnat ccnggctnnc nnannaanga ncncnnnann a
Expected Protein Sequence
MAHHHHHHML LPALERILAG RAPATVAVGS RPDPTMVSAL RVGDQIDDDV ALVVTTSGTT GAPKGALLTA AALIASASAT HHRLGGAGNW LLAVPAIHIA GLQVLVRSAL AGTVPVELDV SAGFDITELP RAVDKLGSGR RYTSLVAAQL AKVLGDPAAT AALAELDAVL IGGGPAPRPV LDAGAAAGIR IVRTYGMSET CGGCVYDGVA LDGVQLRVAA DGRIMIGGAT LAKGYRNPVQ PDPFAEPGWF RTDDLGALDD SGVLSVLGRA DDAISTGGLT VLPQLVESAL ATHAAVSDCA VFGLDDDRLG QRVAAAIVVR AHCPTPTLDQ LRAHVAGTLA ITAAPRELHV VDELPRRGIG KIDRRALVRR FAAATDQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgt tgcccgccct ggagcggatt cttgccggcc gggctccggc aacggtcgcg gtggggtcaa gacctgaccc cacgatggtg agcgcgttgc gggtcggcga ccagatcgat gacgatgtgg cgctggtcgt cacgacgtcg ggaaccaccg gggcgcccaa gggggcgctc ctgaccgcgg ccgcgttgat cgccagcgcg tcggcgaccc atcaccgcct cggcggagcc ggcaactggc tactggccgt gcccgcgatt cacatcgccg gcctgcaggt cctggtgcgc agcgcgctcg ccggtaccgt cccggtcgaa ctcgatgtgt ccgccgggtt cgacatcacc gaactgcccc gcgctgtcga caagttgggt tccggccggc gatacacctc gctggtcgcc gctcagctgg ccaaggtgct gggcgacccc gccgccaccg ccgccctggc cgaactggac gcggtgctga tcgggggtgg accggctccg cgtccggtgc tcgacgccgg tgccgccgcc ggcatccgga tcgttcgcac ctacgggatg agcgaaacct gcggaggctg cgtgtacgac ggtgttgccc tcgacggcgt gcaactgcgc gtggccgcgg acggccgcat catgatcggc ggcgcaacgc tcgccaaggg gtaccgcaac ccggtacaac ccgacccgtt cgccgagccc ggctggtttc gtaccgacga cctcggggcg ctcgatgatt cgggcgtgct cagcgtgctg ggccgcgccg acgacgcgat cagcacgggc gggctcaccg tgctgccgca gctggtggaa tccgcgctgg ccacccacgc cgctgtgagc gactgcgcgg tgttcggact cgacgatgac cggctgggtc agcgggtcgc cgcggcgatc gtggtccgcg cccactgccc caccccaacg ctggatcaac ttcgggcaca cgtcgccggc acgctggcca tcaccgccgc accccgagaa ctgcacgtcg tcgacgagct gccgcggcgc ggcatcggca agatcgatcg gcgggcgttg gtgcgtcgat tcgccgccgc aaccgaccaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat