MyfoA.00629.b

O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26) (Rv0542c ortholog)

CENTER ID: MyfoA.00629.b
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.00629.b.B1.GE37900 full length 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.5461
RefSeq: WP_003883600.1
UniProt: K0V6A2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPADDARQSA LLTTTLRAGQ AIDDDVAVVV STSGTTGTPK GALLTADALR ASATATHARL GGDGRWLLAL PAYHIAGLQV LVRSTLAGTT PVTVPASFDP RELPSAVAAL GSGRRYASLV AVQLDKCLQA PAAAEALADL DAVLIGGGPM PAGMSERAEA AGVTVVRTYG MSETAGGCVY DGVPLDGVEV RIDNSRVLLG GATVAKGYRN PVSPDPFAEP GWFRTDDLGA VDDSGVLRVL GRVDDAVSTG GLTVLPQLVE AALADHPAIA DCAVFGVPDE RLGQRVVAAL VLKPGTSAPD VAELRAHVAR THDATAAPRE VHIVDELPRR GIGKLDRRAL AARYKG
NT Sequence
gtgcccgccg acgatgcccg ccagagcgct ttgctgacaa ccactttgcg tgccgggcag gccatcgacg acgacgtcgc cgtggtggtc tcgacgtccg gcaccaccgg tactcccaaa ggcgcgctgc tgaccgccga tgcgctgcgc gccagcgcga cggccaccca cgcccggctg ggcggtgacg ggaggtggct gctcgcgctg cccgcctatc acattgccgg tcttcaggtt ctggtacgca gcacgctggc cggcaccact cccgtcaccg tgccggcatc cttcgatccg cgtgaattgc cttccgcggt ggccgctctg ggcagcggac ggcgctatgc ctcgctcgtg gcggtccagt tggacaagtg tttgcaggcc ccggccgccg cggaggcact ggcagatctc gacgccgtgc tgatcggggg cgggccgatg ccggctggga tgagcgaacg cgcagaagcc gccggggtga ccgtcgtgcg cacctatggg atgagcgaga ccgcgggcgg ctgcgtgtac gacggcgtgc cactcgacgg cgtggaggtg cgtatcgaca actcgcgcgt cctgctcggc ggggccaccg tggccaaggg ctatcgcaac ccggtcagtc ctgacccgtt tgccgaacca ggctggttcc gcaccgacga ccttggcgcc gtggacgatt caggcgtgct tcgggttttg ggacgggtcg acgacgcggt cagcaccggc gggctgacgg tgttgccgca gctggtcgag gcggcacttg ccgaccatcc cgcgatcgcg gactgcgccg tgttcggggt ccccgacgag cggctcggcc agcgggtggt ggccgcgctg gtcctgaaac ccggcacatc ggcacccgac gtggccgagc tgcgcgccca tgtggcgcgg acccacgacg cgaccgcagc gccgcgcgag gtgcacatcg tcgacgagtt gccccgccgc ggcatcggca agctggaccg gcgtgcgctg gccgcccggt acaagggctg a
Details for MyfoA.00629.b.B1.GE37900
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 36kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 55
Validated AA Sequence
MPAGMSERAE AAGVTVVRTY GMSETAGGCV YDGVPLDGVE VRIDNSRVLL GGATVAKGYR NPVSPDPFAE PGWFRTDDLG AVDDSGVLRV LGRVDDAVST GGLTVLPQLV EAALADHPAI ADCAVFGVPD ERLGQRVVAA LVLKPGTSAP DVAELRAHVA RTHDATAAPR EVHIVDELPR RGIGKLDRRA LAARYKG
Validated NT Sequence
atcttactcn cttagccctt gtaccgggcg gccagcgcac gccggtccag cttgccgatg ccgcggcggg gcaactcgtc gacgatgtgc acctcgcgcg gcgctgcggt cgcgtcgtgg gtccgcgcca catgggcgcg cagctcggcc acgtcgggtg ccgatgtgcc gggtttcagg accagcgcgg ccaccacccg ctggccgagc cgctcgtcgg ggaccccgaa cacggcgcag tccgcgatcg cgggatggtc ggcaagtgcc gcctcgacca gctgcggcaa caccgtcagc ccgccggtgc tgaccgcgtc gtcgacccgt cccaaaaccc gaagcacgcc tgaatcgtcc acggcgccaa ggtcgtcggt gcggaaccag cctggttcgg caaacgggtc aggactgacc gggttgcgat agcccttggc cacggtggcc ccgccgagca ggacgcgcga gttgtcgata cgcacctcca cgccgtcgag tggcacgccg tcgtacacgc agccgcccgc ggtctcgctc atcccatagg tgcgcacgac ggtcaccccg gcggcttctg cgcgttcgct catcccagcc ggcatcggcc cgcccccgat cagcacggcg tcgagatctg ccagtgcctc cgcggcggcc ggggcctgca aacacttgtc caactggacc gccacgagcg aggcatagcg ccgtccgctg cccagagcgg ccaccgcgga aggcaattca cgcggatcga aggatgccgg cacggtgacg ggagtggtgc cggccagcgt gctgcgtacc agaacctgaa gaccggcaat gtgataggcg ggcagcgcga gcagccacct cccgtcaccg cccagccggg cgtgggtggc cgtcgcgctg gcgcgcagcg catcggcggt cagcagcgcg cctttgggag taccggtggt gccggacgtc gagaccacca cggcgacgtc gtcgtcgatg gcctgcccgg cacgcaaagt ggttgtcagc aaagcgctct ggcgggcatc gtcggcgggc a
Expected Protein Sequence
MAHHHHHHMP ADDARQSALL TTTLRAGQAI DDDVAVVVST SGTTGTPKGA LLTADALRAS ATATHARLGG DGRWLLALPA YHIAGLQVLV RSTLAGTTPV TVPASFDPRE LPSAVAALGS GRRYASLVAV QLDKCLQAPA AAEALADLDA VLIGGGPMPA GMSERAEAAG VTVVRTYGMS ETAGGCVYDG VPLDGVEVRI DNSRVLLGGA TVAKGYRNPV SPDPFAEPGW FRTDDLGAVD DSGVLRVLGR VDDAVSTGGL TVLPQLVEAA LADHPAIADC AVFGVPDERL GQRVVAALVL KPGTSAPDVA ELRAHVARTH DATAAPREVH IVDELPRRGI GKLDRRALAA RYKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcccg ccgacgatgc ccgccagagc gctttgctga caaccacttt gcgtgccggg caggccatcg acgacgacgt cgccgtggtg gtctcgacgt ccggcaccac cggtactccc aaaggcgcgc tgctgaccgc cgatgcgctg cgcgccagcg cgacggccac ccacgcccgg ctgggcggtg acgggaggtg gctgctcgcg ctgcccgcct atcacattgc cggtcttcag gttctggtac gcagcacgct ggccggcacc actcccgtca ccgtgccggc atccttcgat ccgcgtgaat tgccttccgc ggtggccgct ctgggcagcg gacggcgcta tgcctcgctc gtggcggtcc agttggacaa gtgtttgcag gccccggccg ccgcggaggc actggcagat ctcgacgccg tgctgatcgg gggcgggccg atgccggctg ggatgagcga acgcgcagaa gccgccgggg tgaccgtcgt gcgcacctat gggatgagcg agaccgcggg cggctgcgtg tacgacggcg tgccactcga cggcgtggag gtgcgtatcg acaactcgcg cgtcctgctc ggcggggcca ccgtggccaa gggctatcgc aacccggtca gtcctgaccc gtttgccgaa ccaggctggt tccgcaccga cgaccttggc gccgtggacg attcaggcgt gcttcgggtt ttgggacggg tcgacgacgc ggtcagcacc ggcgggctga cggtgttgcc gcagctggtc gaggcggcac ttgccgacca tcccgcgatc gcggactgcg ccgtgttcgg ggtccccgac gagcggctcg gccagcgggt ggtggccgcg ctggtcctga aacccggcac atcggcaccc gacgtggccg agctgcgcgc ccatgtggcg cggacccacg acgcgaccgc agcgccgcgc gaggtgcaca tcgtcgacga gttgccccgc cgcggcatcg gcaagctgga ccggcgtgcg ctggccgccc ggtacaaggg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat