MymaA.00629.d

O-succinylbenzoic acid-CoA ligase, MenE (Rv0542c ortholog)

CENTER ID: MymaA.00629.d
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00629.d.B1.GE37192 full length 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.949
RefSeq: YP_001849200.1
UniProt: B2HRN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLPALERIL AGRAPATVAV GSRPDPTVVS ALRVGDQIDD DVALVVTTSG TTGAPKGALL TAAALIASAS ATHHRLGGAG NWLLAVPAIH IAGLQVLVRS ALAGTVPVEL DVSAGFDITE LPRAVDKLGS GRRYTSLVAA QLAKVLGDPA ATAALAELDA VLIGGGPAPR PVLDAAAAAG IRIVRTYGMS ETCGGCVYDG VALDGVQLRV AADGRIMIGG ATLAKGYRNP VQPDPFAEPG WFRTDDLGAL DDSGVLSVLG RADDAISTGG LTVLPQLVES ALATHAAVSD CAVFGLDDDR LGQRVAAAIV VRAHCPTPTL DQLRAHVAGT LAITAAPREL HVVDELPRRG IGKIDRRALV RRFAAATDQ
NT Sequence
gtgctgttgc ccgccctgga gcggattctt gccggccggg ctccggcaac ggtcgcggtg gggtcaagac ctgaccccac ggtggtgagc gcgttgcggg tcggcgacca gatcgatgac gatgtggcgc tggtcgtcac gacgtcggga accaccgggg cgcccaaggg ggcgctcctg accgcggccg cgttgatcgc cagcgcgtcg gcgacccatc accgcctcgg cggagccggc aactggctac tggccgtgcc cgcgattcac atcgccggcc tgcaggtcct ggtgcgcagc gcgctcgccg gtaccgtccc ggtcgaactc gatgtgtccg ccgggttcga catcaccgaa ctccctcgcg ctgtcgacaa gttgggttcc ggccggcgat atacctcgct ggtcgccgct cagctggcca aggtgctggg cgaccccgcc gccaccgccg ccctggccga gctggacgcg gtgctgatcg ggggtggacc ggctccgcgt ccggtgctcg acgccgctgc cgccgccggc atccggatcg ttcgcaccta cgggatgagc gaaacctgcg gaggctgcgt gtacgacggt gttgccctcg acggcgtgca actgcgcgtg gccgcggacg gccgcatcat gatcggcggc gcaacgctgg ccaaggggta ccgcaacccg gtacaacccg acccgttcgc cgagcccggc tggtttcgta ccgacgacct cggggcgctc gatgattcgg gcgtgctcag cgtgctgggc cgcgccgacg acgcgatcag cacgggcggg ctcaccgtgc tgccgcagct ggtggaatcc gcgctggcca cccacgccgc ggtgagcgac tgcgcggtgt tcggactcga cgatgaccgg ctgggtcagc gggtcgccgc ggcgatcgtg gtccgcgccc actgccccac cccaacgctg gatcaacttc gggcacacgt cgccggcacg ctggccatca ccgccgcacc ccgagaactg cacgtcgtcg acgagctgcc gcggcgcggc atcggcaaga tcgatcggcg ggcgttggtg cgtcgattcg ccgccgcaac cgaccaatag
Details for MymaA.00629.d.B1.GE37192
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 45
Validated AA Sequence
MSETCGGCVY DGVALDGVQL RVAADGRIMI GGATLAKGYR NPVQPDPFAE PGWFRTDDLG ALDDSGVLSV LGRADDAIST GGLTVLPQLV ESALATHAAV SDCAVFGLDD DRLGQRVAAA IVVRAHCPTP TLDQLRAHVA GTLAITAAPR ELHVVDELPR RGIGKIDRRA L
Validated NT Sequence
caccaacgcc cgccgatcga tcttgccgat gccgcgccgc ggcagctcgt cgacgacgtg cagttctcgg ggtgcggcgg tgatggccag cgtgccggcg acgtgtgccc gaagttgatc cagcgttggg gtggggcagt gggcgcggac cacgatcgcc gcggcgaccc gctgacccag ccggtcatcg tcgagtccga acaccgcgca gtcgctcacc gcggcgtggg tggccagcgc ggattccacc agctgcggca gcacggtgag cccgcccgtg ctgatcgcgt cgtcggcgcg gcccagcacg ctgagcacgc ccgaatcatc gagcgccccg aggtcgtcgg tacgaaacca gccgggctcg gcgaacgggt cgggttgtac cgggttgcgg taccccttgg ccagcgttgc gccgccgatc atgatgcggc cgtccgcggc cacgcgcagt tgcacgccgt cgagggcaac accgtcgtac acgcagcctc cgcaggtttc gctcatcccg taggtgcgaa cgatccggat gccggcggcg gcagcggcgt cgagcaccgg acgcggagcc ggtccacccc cgatcagcac cgcgtccagc tcggccaggg cggcggtggc ggcggggtcg cccagcacct tggccagctg agcggcgacc agcgaggtat atcgccggcc ggaacccaac ttgtcgacag cgcgagggag ttcggtgatg tcgaacccgg cggacacatc gagttcgacc gggacggtac cggcgagcgc gctgcgcacc aggacctgca ggccggcgat gtgaatcgcg ggcacggcca gtagccagtt gccggctccg ccgaggcggt gatgggtcgc cgacgcgctg gcgatcaacg cggccgcggt caggagcgcc cccttgggcg ccccggtggt tcccgacgtc gtgacgacca gcgccacatc gtcatcgatc tggtcgccga cccgcaacgc gctcaccacc gtggggtcag gtcttgaccc caccgcgacc gttgccggag cccggccggc aagaatccgc tccagggcgg gcaa
Expected Protein Sequence
MAHHHHHHML LPALERILAG RAPATVAVGS RPDPTVVSAL RVGDQIDDDV ALVVTTSGTT GAPKGALLTA AALIASASAT HHRLGGAGNW LLAVPAIHIA GLQVLVRSAL AGTVPVELDV SAGFDITELP RAVDKLGSGR RYTSLVAAQL AKVLGDPAAT AALAELDAVL IGGGPAPRPV LDAAAAAGIR IVRTYGMSET CGGCVYDGVA LDGVQLRVAA DGRIMIGGAT LAKGYRNPVQ PDPFAEPGWF RTDDLGALDD SGVLSVLGRA DDAISTGGLT VLPQLVESAL ATHAAVSDCA VFGLDDDRLG QRVAAAIVVR AHCPTPTLDQ LRAHVAGTLA ITAAPRELHV VDELPRRGIG KIDRRALVRR FAAATDQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgt tgcccgccct ggagcggatt cttgccggcc gggctccggc aacggtcgcg gtggggtcaa gacctgaccc cacggtggtg agcgcgttgc gggtcggcga ccagatcgat gacgatgtgg cgctggtcgt cacgacgtcg ggaaccaccg gggcgcccaa gggggcgctc ctgaccgcgg ccgcgttgat cgccagcgcg tcggcgaccc atcaccgcct cggcggagcc ggcaactggc tactggccgt gcccgcgatt cacatcgccg gcctgcaggt cctggtgcgc agcgcgctcg ccggtaccgt cccggtcgaa ctcgatgtgt ccgccgggtt cgacatcacc gaactccctc gcgctgtcga caagttgggt tccggccggc gatatacctc gctggtcgcc gctcagctgg ccaaggtgct gggcgacccc gccgccaccg ccgccctggc cgagctggac gcggtgctga tcgggggtgg accggctccg cgtccggtgc tcgacgccgc tgccgccgcc ggcatccgga tcgttcgcac ctacgggatg agcgaaacct gcggaggctg cgtgtacgac ggtgttgccc tcgacggcgt gcaactgcgc gtggccgcgg acggccgcat catgatcggc ggcgcaacgc tggccaaggg gtaccgcaac ccggtacaac ccgacccgtt cgccgagccc ggctggtttc gtaccgacga cctcggggcg ctcgatgatt cgggcgtgct cagcgtgctg ggccgcgccg acgacgcgat cagcacgggc gggctcaccg tgctgccgca gctggtggaa tccgcgctgg ccacccacgc cgcggtgagc gactgcgcgg tgttcggact cgacgatgac cggctgggtc agcgggtcgc cgcggcgatc gtggtccgcg cccactgccc caccccaacg ctggatcaac ttcgggcaca cgtcgccggc acgctggcca tcaccgccgc accccgagaa ctgcacgtcg tcgacgagct gccgcggcgc ggcatcggca agatcgatcg gcgggcgttg gtgcgtcgat tcgccgccgc aaccgaccaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat