SSGCID: Target Page

MyleA.00629.b

POSSIBLE O-SUCCINYLBENZOIC ACID--CoA LIGASE MENE (OSB-CoA SYNTHETASE) (O-SUCCINYLBENZOATE-CoA SYNTHASE)

CENTER ID:	MyleA.00629.b
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00629.b.A1.GE34167	full length		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4329
RefSeq:	YP_002504098.1
UniProt:	A0A0H3N0R7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLPTLERIL DGRAPAMVVV PAQHEPIAAL RALRVGEEID DDVALVATTS GTTGVPKGAL LTAAALTASA SATHDRLGGP GSWLLALPPY HIGGLQVLVR SVLAGSVPIE LDISAGFDLA KLPDAVGRLG TGRRYTSLVA TQLAKALTDS TATAVLAELD AVLVGGGPAP RPILDAATSA GIMVVGTYGM SETAGGCVYD GVPLDGVCIR VLVNGHVAIG GATLAKGYRN PIVPDPFAES GWFHTDDLGT VDGSGVLTVL GRADDAISTG GLTVLPGPVE AALCTHPAVS DCAVFGLTDD RLGQRVVAAV VLTDGYATPT LSMLRAHVTR RLDAMAAPRE LHIVDTLPRR GIGKVDRTAL VRRFAKSG

NT Sequence

ttgttgctgc ccacgctgga acgaatcctg gatggccgcg ctcccgcaat ggtcgtggta ccggctcagc atgaacccat cgctgcgctg cgtgctttgc gagtaggtga agagatcgac gacgatgtgg ctttagtggc caccacgtcg ggaacgacag gtgtgcccaa gggcgccttg ctgactgccg ccgccttgac tgccagcgcg tcggccaccc acgaccgcct tggtggtccg ggcagctggc tgctggcttt gccgccgtat catatcggcg ggcttcaggt gctggtgcgt agcgtgctgg ccggttcggt acccatcgag ttggacattt ctgccgggtt tgacctcgcc aaactgccgg acgcggtggg tcgattgggt accggtcgac gatacacgtc actggtcgct acccagctgg ccaaggcgct caccgactca acggccacgg ccgtgctagc cgaattggat gccgtcctcg ttggtggcgg accagctccc cggccgatcc tggatgccgc aacatctgcc ggcatcatgg tggtcggcac ctatgggatg agcgagactg cgggaggttg cgtttacgac ggcgtcccgc tggatggtgt gtgcatacgc gtactggtca atggccacgt agcgatcggt ggtgcgacct tggccaaggg ctatcgcaat ccgatcgttc ccgacccgtt cgccgaatcg ggttggttcc acacagacga cctcggtact gtcgatggct cgggtgtgct aacagtgctg ggtcgggccg acgacgcgat cagcacgggt gggctaaccg tgctgccagg acccgtcgaa gctgcgctgt gcacccatcc cgcggtcagc gactgtgcgg tgttcgggct tactgacgac cggctaggtc agcgggtagt agccgcagtg gtactgactg acggttacgc aacgccgacg ctgagcatgt tgcgggcaca tgtcacccgc agactggacg ccatggccgc accgcgcgag ctacacatcg ttgatacact gccacgacgt ggcatcggaa aggtcgatcg cacggcgtta gtgcgccggt tcgccaagtc cggttaa

Details for MyleA.00629.b.A1.GE34167

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	75

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLLLPTLER ILDGRAPAMV VVPAQHEPIA ALRALRVGEE IDDDVALVAT TSGTTGVPKG ALLTAAALTA SASATHDRLG GPGSWLLALP PYHIGGLQVL VRSVLAGSVP IELDISAGFD LAKLPDAVGR LGTGRRYTSL VATQLAKALT DSTATAVLAE LDAVLVGGGP APRPILDAAT SAGIMVVGTY GMSETAGGCV YDGVPLDGVC IRVLVNGHVA IGGATLAKGY RNPIXPXPXA ESGWFHTDDL GTVDGSGVLT VLGRADXXIS TGX

Validated NT Sequence

attttgtnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt tgctgcccac gctggaacga atcctggatg gccgcgctcc cgcaatggtc gtggtaccgg ctcagcatga acccatcgct gcgctgcgtg ctttgcgagt aggtgaagag atcgacgacg atgtggcttt agtggccacc acgtcgggaa cgacaggtgt gcccaagggc gccttgctga ctgccgccgc cttgactgcc agcgcgtcgg ccacccacga ccgccttggt ggtccgggca gctggctgct ggctttgccg ccgtatcata tcggcgggct tcaggtgctg gtgcgtagcg tgctggccgg ttcggtaccc atcgagttgg acatttctgc cgggtttgac ctcgccaaac tgccggacgc ggtgggtcga ttgggtaccg gtcgacgata cacgtcactg gtcgctaccc agctggccaa ggcgctcacc gactcaacgg ccacggccgt gctagccgaa ttggatgccg tcctcgttgg tggcggacca gctccccggc cgatcctgga tgccgcaaca tctgccggca tcatggtggt cggcacctat gggatgagcg agactgcggg aggttgcgtt tacgacggcg tcccgctgga tggtgtgtgc atacgcgtac tggtcaatgg ccacgtagcg atcggtggtg cgaccttggc caagggctat cgcaatccga tcgntcccna cccgntcgcc gaatcgggtt ggttccacac agacgacctc ggtactgtcg atggntcggg tgtgctaaca gtgctgggtc gggccgacna cncgatcagc acgggtgnnt aaccgtgctg ccnggacccn tcgaanctnn nntgtnnacc natcccnngn cagcnactgn gngnntnngg ctntgannnn nntngncagn ngnagnancn canngnactg actgannnnc ancnccnann ctnancnnnn nnngnnnntn nnnnnnnnnn ngnnnnccnn nnnnnnnnnn nnnnnnnnnn nngccntnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnanna nnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgttgctg cccacgctgg aacgaatcct ggatggccgc gctcccgcaa tggtcgtggt accggctcag catgaaccca tcgctgcgct gcgtgctttg cgagtaggtg aagagatcga cgacgatgtg gctttagtgg ccaccacgtc gggaacgaca ggtgtgccca agggcgcctt gctgactgcc gccgccttga ctgccagcgc gtcggccacc cacgaccgcc ttggtggtcc gggcagctgg ctgctggctt tgccgccgta tcatatcggc gggcttcagg tgctggtgcg tagcgtgctg gccggttcgg tacccatcga gttggacatt tctgccgggt ttgacctcgc caaactgccg gacgcggtgg gtcgattggg taccggtcga cgatacacgt cactggtcgc tacccagctg gccaaggcgc tcaccgactc aacggccacg gccgtgctag ccgaattgga tgccgtcctc gttggtggcg gaccagctcc ccggccgatc ctggatgccg caacatctgc cggcatcatg gtggtcggca cctatgggat gagcgagact gcgggaggtt gcgtttacga cggcgtcccg ctggatggtg tgtgcatacg cgtactggtc aatggccacg tagcgatcgg tggtgcgacc ttggccaagg gctatcgcaa tccgatcgtt cccgacccgt tcgccgaatc gggttggttc cacacagacg acctcggtac tgtcgatggc tcgggtgtgc taacagtgct gggtcgggcc gacgacgcga tcagcacggg tgggctaacc gtgctgccag gacccgtcga agctgcgctg tgcacccatc ccgcggtcag cgactgtgcg gtgttcgggc ttactgacga ccggctaggt cagcgggtag tagccgcagt ggtactgact gacggttacg caacgccgac gctgagcatg ttgcgggcac atgtcacccg cagactggac gccatggccg caccgcgcga gctacacatc gttgatacac tgccacgacg tggcatcgga aaggtcgatc gcacggcgtt agtgcgccgg ttcgccaagt ccggttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa