MythA.00629.f

O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26) (Rv0542c ortholog)

CENTER ID: MythA.00629.f
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.f.B2.GE37641 mature protein 23 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.2976
UniProt: G7CHD4

Orthologues for Rv0542c

Species % Identity % Coverage Clones Proteins Structures
marinum 79 100 1 0 0
ulcerans 79 100 1 0 0
paratuberculosis 78 100 0 0 0
leprae 78 99 1 0 0
avium 77 100 0 0 0
fortuitum 68 95 1 0 0
smegmatis 68 95 0 0 0
thermoresistibile 65 98 1 0 0
abscessus 61 99 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLRPISVA AGAEALAILP ALADALEDRS PALLPVPADD ARDRALLTTA LRAGEPIDDD VAVVVATSGT TGTPKGAMLT AVALRASAEA THNRLGGPGQ WLLALPAEHI AGLQVLVRSL IAGTTPVAIA PNFAPADLVA AIDQMDPGTR RYTAMVAAQL AKVLHHDEAT AALARLDAVL IGGGPIPAAV AEPAVAAGIN VVRTYGMSET AGGCVYDGFP LDGVRVRLDD SRILLGGPVL AKGYRNPVWP DPFAEPGWFR TDDYGLIDES GALRVLGRVD DAISTGGLTV LPQVVEAAVN THPAVAESVV FGVPDDRLGQ RVVAALVLKP GAEAPSVDDL RTHVARILDR TAAPREIHIL TRLPRRGIGK VDRRALAQRF AS
NT Sequence
atgagcgtgc tgcgtccgat atccgtcgcc gccggcgctg aggcgctggc gatactgccc gccctggccg atgcgctgga agaccgttct ccggcgctgc tgccggtccc cgccgacgac gcccgcgacc gcgcactgct gaccaccgcc ctgcgggccg gtgaacccat cgacgacgac gtcgcggtgg tggtggcgac ctcgggaacc accggcaccc ccaagggcgc catgctgacc gccgtggcgt tgcgggccag cgccgaggcc acccacaacc gtctcggcgg gcccggtcag tggttgctgg cgctgcccgc cgaacacatc gccggactgc aggtgctggt gcgcagcctg atcgccggca ccacaccggt cgcgatcgcc ccgaacttcg cgccggccga tctggtggcg gccatcgacc agatggaccc cggcacccgg cgctacaccg cgatggtggc cgcacaactg gcgaaggtgc tgcaccacga cgaggccacc gcggcgctgg cccggttgga cgcggtgttg atcggtggcg ggccgatacc cgcggcggtg gccgaaccgg ccgtcgccgc cgggatcaac gtggtgcgca cctacgggat gagcgagacc gccggcggct gcgtgtacga cggcttcccg ctcgacgggg tgcgggtgcg gctcgacgac tcgcgcatcc tgctgggcgg gccggtgctg gcgaagggct accgcaatcc ggtgtggccc gacccgttcg ccgagccggg ctggttccgc accgatgact acgggctcat cgatgaatcg ggcgcgctgc gggtgctggg ccgggtcgac gacgcgatca gcaccggtgg gctgaccgtg ttgccgcagg tggtggaggc ggcggtgaac acccacccgg cggtggccga gagcgtggtg ttcggggtgc ccgacgaccg gctggggcag cgggtggtgg ccgcgctggt gctcaaaccc ggcgccgagg cgccgtcggt ggacgacctg cgcacccacg tcgcccggat cctggaccgg accgccgcgc cccgcgagat ccacatcctg accaggttgc cgcgccgcgg catcggcaag gtcgatcggc gcgcgttggc ccagcggttc gccagctga
Details for MythA.00629.f.B2.GE37641
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 80
Validated AA Sequence
MLTAVALRAS AEATHNRLGG PGQWLLALPA EHIAGLQVLV RSLIAGTTPV AIAPNFAPAD LVAAIDQMDP GTRRYTAMVA AQLAKVLHHD EATAALARLD AVLIGGGPIP AAVAEPAVAA GINVVRTYGM SETAGGCVYD GFPLDGVRVR LDDSRILLGG PVLAKGYRNP VWPDPFAEPG WFRTDDYGLI DESGALRVLG RVDDAISTGG LTVLPQXVEA AVNTHPAVAE SVVFGVPDDR LGQRVVAALV LKPGAEAXSV DDLRTHVARI LDRTXAPREI HIXTRXXRRG XGXXDR
Validated NT Sequence
cgccgatcnn nnttgccnat gccncggcgc gncnncctgg tcnngatgtg gatctcgcgg ggcgcgncgg tccggtccag gatccgggcg acgtgggtgc gcaggtcgtc caccgacngn gcctcggcgc cnggtttgag caccagcgcg gccaccaccc gctgccccag ccggtcgtcg ggcaccccga acaccacgct ctcggccacc gccgggtggg tgttcaccgc cgcctccacc ncctgcggca acacggtcag cccaccggtg ctgatcgcgt cgtcgacccg gcccagcacc cgcagcgcgc ccgattcatc gatgagcccg tagtcatcgg tgcggaacca gcccggctcg gcgaacgggt cgggccacac cggattgcgg tagcccttcg ccagcaccgg cccgcccagc aggatgcgcg agtcgtcgag ccgcacccgc accccgtcga gcgggaagcc gtcgtacacg cagccgccgg cggtctcgct catcccgtag gtgcgcacca cgttgatccc ggcggcgacg gccggttcgg ccaccgccgc gggtatcggc ccgccaccga tcaacaccgc gtccaaccgg gccagcgccg cggtggcctc gtcgtggtgc agcaccttcg ccagttgtgc ggccaccatc gcggtgtagc gccgggtgcc ggggtccatc tggtcgatgg ccgccaccag atcggccggc gcgaagttcg gggcgatcgc gaccggtgtg gtgccggcga tcaggctgcg caccagcacc tgcagtccgg cgatgtgttc ggcgggcagc gccagcaacc actgaccggg cccgccgaga cggttgtggg tggcctcggc gctggcccgc aacgccacgg cggtcagcat ggcgcccttg ggggtgccgg tggttcccga ggtcgccacc accaccgcga cgtcgtcgtc gatgggttca ccggcccgca gggcggtggt cagcagtgcg cggtcgcggg cgtcgtcggc ggggaccggc agcagcgccg gagaacggtc ttccagcgca tc
Expected Protein Sequence
MAHHHHHHAD ALEDRSPALL PVPADDARDR ALLTTALRAG EPIDDDVAVV VATSGTTGTP KGAMLTAVAL RASAEATHNR LGGPGQWLLA LPAEHIAGLQ VLVRSLIAGT TPVAIAPNFA PADLVAAIDQ MDPGTRRYTA MVAAQLAKVL HHDEATAALA RLDAVLIGGG PIPAAVAEPA VAAGINVVRT YGMSETAGGC VYDGFPLDGV RVRLDDSRIL LGGPVLAKGY RNPVWPDPFA EPGWFRTDDY GLIDESGALR VLGRVDDAIS TGGLTVLPQV VEAAVNTHPA VAESVVFGVP DDRLGQRVVA ALVLKPGAEA PSVDDLRTHV ARILDRTAAP REIHILTRLP RRGIGKVDRR ALAQRFAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatg cgctggaaga ccgttctccg gcgctgctgc cggtccccgc cgacgacgcc cgcgaccgcg cactgctgac caccgccctg cgggccggtg aacccatcga cgacgacgtc gcggtggtgg tggcgacctc gggaaccacc ggcaccccca agggcgccat gctgaccgcc gtggcgttgc gggccagcgc cgaggccacc cacaaccgtc tcggcgggcc cggtcagtgg ttgctggcgc tgcccgccga acacatcgcc ggactgcagg tgctggtgcg cagcctgatc gccggcacca caccggtcgc gatcgccccg aacttcgcgc cggccgatct ggtggcggcc atcgaccaga tggaccccgg cacccggcgc tacaccgcga tggtggccgc acaactggcg aaggtgctgc accacgacga ggccaccgcg gcgctggccc ggttggacgc ggtgttgatc ggtggcgggc cgatacccgc ggcggtggcc gaaccggccg tcgccgccgg gatcaacgtg gtgcgcacct acgggatgag cgagaccgcc ggcggctgcg tgtacgacgg cttcccgctc gacggggtgc gggtgcggct cgacgactcg cgcatcctgc tgggcgggcc ggtgctggcg aagggctacc gcaatccggt gtggcccgac ccgttcgccg agccgggctg gttccgcacc gatgactacg ggctcatcga tgaatcgggc gcgctgcggg tgctgggccg ggtcgacgac gcgatcagca ccggtgggct gaccgtgttg ccgcaggtgg tggaggcggc ggtgaacacc cacccggcgg tggccgagag cgtggtgttc ggggtgcccg acgaccggct ggggcagcgg gtggtggccg cgctggtgct caaacccggc gccgaggcgc cgtcggtgga cgacctgcgc acccacgtcg cccggatcct ggaccggacc gccgcgcccc gcgagatcca catcctgacc aggttgccgc gccgcggcat cggcaaggtc gatcggcgcg cgttggccca gcggttcgcc agctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat