SSGCID: Target Page

MytuD.01407.a

Probable dipeptidase PepE

CENTER ID:	MytuD.01407.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01407.a.A1.GE25965	Full length( MytuD.01407.a )		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01407.a.A1.PS00179a	Full length( MytuD.01407.a )		1	375

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126673
PATRIC ID:	fig\|83332.12.peg.2334
RefSeq:	NP_216605.1
TubercuList:	Rv2089c
MTB Network Portal:	Rv2089c
UniProt:	P9WHS7

Orthologues for Rv2089c

Species	% Identity	% Coverage	Clones	Proteins	Structures
marinum	85	100	3	1	0
ulcerans	85	100	1	1	1
avium	84	100	0	0	0
paratuberculosis	84	100	1	1	0
smegmatis	78	100	0	0	0
abscessus	73	99	1	1	0
smegmatis	29	91	0	1	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSRRFDAEV YARRLALAAA ATADAGLAGL VITPGYDLCY LIGSRAETFE RLTALVLPAA GAPAVVLPRL ELAALKQSAA AELGLRVCDW VDGDDPYGLV SAVLGGAPVA TAVTDSMPAL HMLPLADALG VLPVLATDVL RRLRMVKEET EIDALRKAGA AIDRVHARVP EFLVPGRTEA DVAADIAEAI VAEGHSEVAF VIVGSGPHGA DPHHGYSDRE LREGDIVVVD IGGTYGPGYH SDSTRTYSIG EPDSDVAQSY SMLQRAQRAA FEAIRPGVTA EQVDAAARDV LAEAGLAEYF VHRTGHGIGL CVHEEPYIVA GNDLVLVPGM AFSIEPGIYF PGRWGARIED IVIVTEDGAV SVNNCPHELI VVPVS

NT Sequence

ATGGGTTCTC GCCGATTCGA CGCCGAGGTT TATGCACGGC GGCTGGCTTT AGCGGCGGCC GCGACGGCGG ACGCCGGTCT GGCGGGTCTG GTGATAACTC CCGGCTACGA CCTGTGTTAC CTCATCGGGT CGCGAGCGGA GACGTTCGAG CGGCTCACCG CGTTGGTGTT GCCGGCCGCC GGTGCGCCGG CGGTTGTGCT GCCGCGGCTG GAGCTCGCCG CCCTCAAGCA ATCCGCCGCA GCGGAATTGG GTCTGCGCGT GTGCGATTGG GTCGACGGTG ACGACCCCTA CGGGTTGGTG AGTGCCGTGT TGGGCGGAGC TCCGGTAGCG ACCGCGGTCA CCGATTCCAT GCCGGCGTTG CACATGTTGC CGCTGGCCGA CGCACTGGGT GTGCTGCCGG TATTGGCCAC CGACGTGCTG CGCAGGCTGC GGATGGTCAA GGAGGAAACC GAGATCGACG CGCTGCGTAA GGCCGGCGCG GCGATCGATC GAGTGCATGC CCGAGTGCCG GAGTTTCTGG TCCCGGGCCG AACGGAAGCC GACGTAGCCG CCGACATCGC CGAAGCAATT GTCGCCGAAG GGCATTCGGA GGTAGCGTTC GTCATCGTGG GTTCCGGGCC GCACGGCGCC GACCCGCATC ACGGATATTC GGACCGCGAA TTGCGGGAGG GTGACATCGT TGTCGTCGAC ATCGGCGGCA CGTATGGGCC TGGATACCAC TCCGACTCCA CCCGAACCTA CAGCATCGGC GAGCCTGATT CTGATGTAGC GCAGTCATAT TCGATGTTGC AGCGAGCCCA GCGGGCGGCG TTCGAGGCCA TCCGCCCAGG GGTGACAGCG GAGCAGGTGG ACGCCGCCGC GCGTGACGTG CTCGCCGAGG CCGGGCTCGC GGAGTATTTT GTGCACCGCA CCGGGCACGG CATCGGGCTG TGCGTGCACG AGGAGCCCTA TATCGTCGCC GGCAATGACC TGGTGTTGGT TCCCGGCATG GCGTTTTCCA TCGAGCCGGG AATCTATTTC CCGGGCCGGT GGGGCGCCCG CATCGAGGAC ATCGTGATCG TGACCGAGGA CGGTGCTGTG TCTGTCAACA ACTGCCCGCA CGAGTTGATC GTGGTGCCGG TGTCC

Details for MytuD.01407.a.A1.GE25965

HARVESTED ON:	1/9/2009
SEQUENCED ON:	7/28/2010
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG LMGSRRFDAE VYARRLALAA AATADAGLAG LVITPGYDLC YLIGSRAETF ERLTALVLPA AGAPAVVLPR LELAALKQSA AAELGLRVCD WVDGDDPYGL VSAVLGGAPV ATAVTDSMPA LHMLPLADAL GVLPVLATDV LRRLRMVKEE TEIDALRKAG AAIDRVHARV PEFLVPGRTE ADVAADIAEA IVAEGHSEVA FVIVGSGPHG ADPHHGYSDR ELREGDIVVV DIGGTYGPGY HSDSTRTYSI GEPDSDVAQS YSMLQRAQRA AFEAIRPGVT AEQVDAAARD VLAEAGLAEY FVHRTGHGIG LCVHEEPYIV AGNDLVLVPG MAFSIEPGIY FPGRWGARIE DIVIVTEDGA VSVNNCPHEL IVVPVS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt ttgatgggtt ctcgccgatt cgacgccgag gtttatgcac ggcggctggc tttagcggcg gccgcgacgg cggacgccgg tctggcgggt ctggtgataa ctcccggcta cgacctgtgt tacctcatcg ggtcgcgagc ggagacgttc gagcggctca ccgcgttggt gttgccggcc gccggtgcgc cggcggttgt gctgccgcgg ctggagctcg ccgccctcaa gcaatccgcc gcagcggaat tgggtctgcg cgtgtgcgat tgggtcgacg gtgacgaccc ctacgggttg gtgagtgccg tgttgggcgg agctccggta gcgaccgcgg tcaccgattc catgccggcg ttgcacatgt tgccgctggc cgacgcactg ggtgtgctgc cggtattggc caccgacgtg ctgcgcaggc tgcggatggt caaggaggaa accgagatcg acgcgctgcg taaggccggc gcggcgatcg atcgagtgca tgcccgagtg ccggagtttc tggtcccggg ccgaacggaa gccgacgtag ccgccgacat cgccgaagca attgtcgccg aagggcattc ggaggtagcg ttcgtcatcg tgggttccgg gccgcacggc gccgacccgc atcacggata ttcggaccgc gaattgcggg agggtgacat cgttgtcgtc gacatcggcg gcacgtatgg gcctggatac cactccgact ccacccgaac ctacagcatc ggcgagcctg attctgatgt agcgcagtca tattcgatgt tgcagcgagc ccagcgggcg gcgttcgagg ccatccgccc aggggtgaca gcggagcagg tggacgccgc cgcgcgtgac gtgctcgccg aggccgggct cgcggagtat tttgtgcacc gcaccgggca cggcatcggg ctgtgcgtgc acgaggagcc ctatatcgtc gccggcaatg acctggtgtt ggttcccggc atggcgtttt ccatcgagcc gggaatctat ttcccgggcc ggtggggcgc ccgcatcgag gacatcgtga tcgtgaccga ggacggtgct gtgtctgtca acaactgccc gcacgagttg atcgtggtgc cggtgtccta at

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGSRRFDAE VYARRLALAA AATADAGLAG LVITPGYDLC YLIGSRAETF ERLTALVLPA AGAPAVVLPR LELAALKQSA AAELGLRVCD WVDGDDPYGL VSAVLGGAPV ATAVTDSMPA LHMLPLADAL GVLPVLATDV LRRLRMVKEE TEIDALRKAG AAIDRVHARV PEFLVPGRTE ADVAADIAEA IVAEGHSEVA FVIVGSGPHG ADPHHGYSDR ELREGDIVVV DIGGTYGPGY HSDSTRTYSI GEPDSDVAQS YSMLQRAQRA AFEAIRPGVT AEQVDAAARD VLAEAGLAEY FVHRTGHGIG LCVHEEPYIV AGNDLVLVPG MAFSIEPGIY FPGRWGARIE DIVIVTEDGA VSVNNCPHEL IVVPVS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggttctcgc cgattcgacg ccgaggttta tgcacggcgg ctggctttag cggcggccgc gacggcggac gccggtctgg cgggtctggt gataactccc ggctacgacc tgtgttacct catcgggtcg cgagcggaga cgttcgagcg gctcaccgcg ttggtgttgc cggccgccgg tgcgccggcg gttgtgctgc cgcggctgga gctcgccgcc ctcaagcaat ccgccgcagc ggaattgggt ctgcgcgtgt gcgattgggt cgacggtgac gacccctacg ggttggtgag tgccgtgttg ggcggagctc cggtagcgac cgcggtcacc gattccatgc cggcgttgca catgttgccg ctggccgacg cactgggtgt gctgccggta ttggccaccg acgtgctgcg caggctgcgg atggtcaagg aggaaaccga gatcgacgcg ctgcgtaagg ccggcgcggc gatcgatcga gtgcatgccc gagtgccgga gtttctggtc ccgggccgaa cggaagccga cgtagccgcc gacatcgccg aagcaattgt cgccgaaggg cattcggagg tagcgttcgt catcgtgggt tccgggccgc acggcgccga cccgcatcac ggatattcgg accgcgaatt gcgggagggt gacatcgttg tcgtcgacat cggcggcacg tatgggcctg gataccactc cgactccacc cgaacctaca gcatcggcga gcctgattct gatgtagcgc agtcatattc gatgttgcag cgagcccagc gggcggcgtt cgaggccatc cgcccagggg tgacagcgga gcaggtggac gccgccgcgc gtgacgtgct cgccgaggcc gggctcgcgg agtattttgt gcaccgcacc gggcacggca tcgggctgtg cgtgcacgag gagccctata tcgtcgccgg caatgacctg gtgttggttc ccggcatggc gttttccatc gagccgggaa tctatttccc gggccggtgg ggcgcccgca tcgaggacat cgtgatcgtg accgaggacg gtgctgtgtc tgtcaacaac tgcccgcacg agttgatcgt ggtgccggtg tccaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MytuD.01407.a.A1.PS00179a

PURIFICATION DATe:	3/4/2009
CONCENTRATION:	42.89mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTTCTCGC CGATTCGACG CCGAGGTTTA TGCACGGCGG CTGGCTTTAG CGGCGGCCGC GACGGCGGAC GCCGGTCTGG CGGGTCTGGT GATAACTCCC GGCTACGACC TGTGTTACCT CATCGGGTCG CGAGCGGAGA CGTTCGAGCG GCTCACCGCG TTGGTGTTGC CGGCCGCCGG TGCGCCGGCG GTTGTGCTGC CGCGGCTGGA GCTCGCCGCC CTCAAGCAAT CCGCCGCAGC GGAATTGGGT CTGCGCGTGT GCGATTGGGT CGACGGTGAC GACCCCTACG GGTTGGTGAG TGCCGTGTTG GGCGGAGCTC CGGTAGCGAC CGCGGTCACC GATTCCATGC CGGCGTTGCA CATGTTGCCG CTGGCCGACG CACTGGGTGT GCTGCCGGTA TTGGCCACCG ACGTGCTGCG CAGGCTGCGG ATGGTCAAGG AGGAAACCGA GATCGACGCG CTGCGTAAGG CCGGCGCGGC GATCGATCGA GTGCATGCCC GAGTGCCGGA GTTTCTGGTC CCGGGCCGAA CGGAAGCCGA CGTAGCCGCC GACATCGCCG AAGCAATTGT CGCCGAAGGG CATTCGGAGG TAGCGTTCGT CATCGTGGGT TCCGGGCCGC ACGGCGCCGA CCCGCATCAC GGATATTCGG ACCGCGAATT GCGGGAGGGT GACATCGTTG TCGTCGACAT CGGCGGCACG TATGGGCCTG GATACCACTC CGACTCCACC CGAACCTACA GCATCGGCGA GCCTGATTCT GATGTAGCGC AGTCATATTC GATGTTGCAG CGAGCCCAGC GGGCGGCGTT CGAGGCCATC CGCCCAGGGG TGACAGCGGA GCAGGTGGAC GCCGCCGCGC GTGACGTGCT CGCCGAGGCC GGGCTCGCGG AGTATTTTGT GCACCGCACC GGGCACGGCA TCGGGCTGTG CGTGCACGAG GAGCCCTATA TCGTCGCCGG CAATGACCTG GTGTTGGTTC CCGGCATGGC GTTTTCCATC GAGCCGGGAA TCTATTTCCC GGGCCGGTGG GGCGCCCGCA TCGAGGACAT CGTGATCGTG ACCGAGGACG GTGCTGTGTC TGTCAACAAC TGCCCGCACG AGTTGATCGT GGTGCCGGTG TCCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA