SSGCID: Target Page

MypaA.01407.a

PepE

CENTER ID:	MypaA.01407.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01407.a.A1.GE29602	Full length( MypaA.01407.a )		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01407.a.A1.PW29502	Full length( MypaA.01407.a )		1	375

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126673
PATRIC ID:	fig\|262316.17.peg.1933
RefSeq:	NP_960757.1
UniProt:	Q73YY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESHRFDADV YARRLAAAAS ATAAAGLGGL VITPGYDLRY LVGSRAQTLE RFTALVLPAS GEPTVVVPRL EVASLKGSAI AELGLPVRDW VDGENPYELV AAALGGAPAA TAVTDSMPAL HLLPLAGVLG VLPVLATDVL RGLRMVKEEC EVDALRKAGA AIDRVHARVP SFLIPGRTEA DVAADIAEAI VAEGHSEVAF IIVGSGPHGA DPHHGYSDRE LQVGDIVVVD IGGSYEPGYH SDSTRTYSIG EPSPEVAQQY SILQRAQRAA CDAVRPGVTA EQVAAAARDV LAAAGLAEYF VHRTGHGIGL SVHEEPYIVA GNDLPLAAGM AFSIEPGIYF PGRWGARIED IVVVTDDGAL AVNKRPHELI VVPVP

NT Sequence

ATGGAATCCC ACCGTTTCGA CGCGGACGTG TATGCGCGCC GGCTGGCGGC GGCCGCCTCG GCGACCGCCG CCGCCGGGCT GGGCGGCCTG GTGATCACGC CGGGGTATGA CCTGCGCTAC CTGGTCGGGT CGCGGGCGCA GACCTTGGAG CGGTTCACCG CCCTGGTGTT GCCCGCCTCC GGTGAGCCGA CCGTCGTGGT GCCGCGGCTG GAGGTGGCCT CGCTGAAGGG GTCGGCGATC GCCGAACTCG GTCTGCCGGT GCGGGATTGG GTCGACGGTG AGAACCCCTA CGAACTGGTG GCCGCGGCGT TGGGCGGGGC GCCCGCCGCG ACCGCCGTCA CCGACTCCAT GCCGGCGCTG CATCTGCTGC CGCTGGCCGG CGTGCTCGGC GTGCTGCCGG TGCTGGCCAC CGACGTGTTG CGCGGCCTGC GGATGGTCAA GGAAGAGTGC GAGGTCGACG CCTTGCGGAA GGCTGGCGCG GCCATCGACC GGGTGCACGC CCGGGTGCCG TCGTTTCTGA TACCGGGCCG CACCGAGGCT GACGTGGCCG CCGATATCGC CGAAGCCATT GTGGCCGAAG GGCATTCGGA GGTGGCCTTC ATCATCGTCG GCTCCGGCCC GCACGGCGCC GACCCGCACC ACGGGTATTC GGATCGCGAG CTGCAGGTGG GAGACATCGT CGTCGTCGAC ATCGGGGGCT CCTACGAGCC GGGCTATCAT TCGGATTCGA CCCGCACCTA CAGCATCGGG GAGCCGAGTC CCGAAGTGGC CCAACAGTAT TCGATTCTGC AGCGCGCTCA GCGGGCGGCC TGTGATGCGG TCCGGCCCGG TGTGACGGCC GAGCAGGTCG CTGCTGCCGC CCGTGACGTG CTGGCCGCCG CGGGACTCGC GGAGTATTTC GTGCACCGCA CGGGGCACGG CATCGGGCTG TCGGTGCACG AAGAGCCTTA CATCGTTGCG GGAAACGACC TGCCGCTCGC CGCGGGCATG GCCTTCTCCA TCGAGCCGGG CATCTATTTC CCGGGGCGGT GGGGCGCTCG CATCGAGGAC ATCGTGGTCG TGACGGACGA CGGCGCCCTG GCTGTCAACA AGCGGCCGCA CGAACTGATC GTGGTGCCGG TGCCG

Details for MypaA.01407.a.A1.GE29602

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MESHRFDADV YARRLAAAAS ATAAAGLGGL VITPGYDLRY LVGSRAQTLE RFTALVLPAS GEPTVVVPRL EVASLKGSAI AELGLPVRDW VDGENPYELV AAALGGAPAA TAVTDSMPAL HLLPLAGVLG VLPVLATDVL RGLRMVKEEC EVDALRKAGA AIDRVHARVP SFLIPGRTEA DVAADIAEAI VAEGHSEVAF IIVGSGPHGA DPHHGYSDRE LQVGDIVVVD IGGSYEPGYH SDSTRTYSIG EPSPEVAQQY SILQRAQRAA CDAVRPGVTA EQVAAAARDV LAAAGLAEYF VHRTGHGIGL SVXEEPYIVA GNDLPLAAGM AFSIEPGIYF PGRWGARIED IVVVTDDGAL AVNKRPHELI VVPVP

Validated NT Sequence

tacggcaccg gcaccacgat cagttcgtgc ggccgcttgt tgacagccag ggcgccgtcg tccgtcacga ccacgatgtc ctcgatgcga gcgccccacc gccccgggaa atagatgccc ggctcgatgg agaaggccat gcccgcggcg agcggcaggt cgtttcccgc aacgatgtaa ggctcttcnt gcaccgacag cccgatgccg tgccccgtgc ggtgcacgaa atactccgcg agtcccgcgg cggccagcac gtcacgggcg gcagcagcga cctgctcggc cgtcacaccg ggccggaccg catcacaggc cgcccgctga gcgcgctgca gaatcgaata ctgttgggcc acttcgggac tcggctcccc gatgctgtag gtgcgggtcg aatccgaatg atagcccggc tcgtaggagc ccccgatgtc gacgacgacg atgtctccca cctgcagctc gcgatccgaa tacccgtggt gcgggtcggc gccgtgcggg ccggagccga cgatgatgaa ggccacctcc gaatgccctt cggccacaat ggcttcggcg atatcggcgg ccacgtcagc ctcggtgcgg cccggtatca gaaacgacgg cacccgggcg tgcacccggt cgatggccgc gccagccttc cgcaaggcgt cgacctcgca ctcttccttg accatccgca ggccgcgcaa cacgtcggtg gccagcaccg gcagcacgcc gagcacgccg gccagcggca gcagatgcag cgccggcatg gagtcggtga cggcggtcgc ggcgggcgcc ccgcccaacg ccgcggccac cagttcgtag gggttctcac cgtcgaccca atcccgcacc ggcagaccga gttcggcgat cgccgacccc ttcagcgagg ccacctccag ccgcggcacc acgacggtcg gctcaccgga ggcgggcaac accagggcgg tgaaccgctc caaggtctgc gcccgcgacc cgaccaggta gcgcaggtca taccccggcg tgatcaccag gccgcccagc ccggcggcgg cggtcgccga ggcggccgcc gccagccggc gcgcatacac gtccgcgtcg aaacggtggg attccatcga accaggaccc tgggtctgag cttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMESHRFDAD VYARRLAAAA SATAAAGLGG LVITPGYDLR YLVGSRAQTL ERFTALVLPA SGEPTVVVPR LEVASLKGSA IAELGLPVRD WVDGENPYEL VAAALGGAPA ATAVTDSMPA LHLLPLAGVL GVLPVLATDV LRGLRMVKEE CEVDALRKAG AAIDRVHARV PSFLIPGRTE ADVAADIAEA IVAEGHSEVA FIIVGSGPHG ADPHHGYSDR ELQVGDIVVV DIGGSYEPGY HSDSTRTYSI GEPSPEVAQQ YSILQRAQRA ACDAVRPGVT AEQVAAAARD VLAAAGLAEY FVHRTGHGIG LSVHEEPYIV AGNDLPLAAG MAFSIEPGIY FPGRWGARIE DIVVVTDDGA LAVNKRPHEL IVVPVP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatcccac cgtttcgacg cggacgtgta tgcgcgccgg ctggcggcgg ccgcctcggc gaccgccgcc gccgggctgg gcggcctggt gatcacgccg gggtatgacc tgcgctacct ggtcgggtcg cgggcgcaga ccttggagcg gttcaccgcc ctggtgttgc ccgcctccgg tgagccgacc gtcgtggtgc cgcggctgga ggtggcctcg ctgaaggggt cggcgatcgc cgaactcggt ctgccggtgc gggattgggt cgacggtgag aacccctacg aactggtggc cgcggcgttg ggcggggcgc ccgccgcgac cgccgtcacc gactccatgc cggcgctgca tctgctgccg ctggccggcg tgctcggcgt gctgccggtg ctggccaccg acgtgttgcg cggcctgcgg atggtcaagg aagagtgcga ggtcgacgcc ttgcggaagg ctggcgcggc catcgaccgg gtgcacgccc gggtgccgtc gtttctgata ccgggccgca ccgaggctga cgtggccgcc gatatcgccg aagccattgt ggccgaaggg cattcggagg tggccttcat catcgtcggc tccggcccgc acggcgccga cccgcaccac gggtattcgg atcgcgagct gcaggtggga gacatcgtcg tcgtcgacat cgggggctcc tacgagccgg gctatcattc ggattcgacc cgcacctaca gcatcgggga gccgagtccc gaagtggccc aacagtattc gattctgcag cgcgctcagc gggcggcctg tgatgcggtc cggcccggtg tgacggccga gcaggtcgct gctgccgccc gtgacgtgct ggccgccgcg ggactcgcgg agtatttcgt gcaccgcacg gggcacggca tcgggctgtc ggtgcacgaa gagccttaca tcgttgcggg aaacgacctg ccgctcgccg cgggcatggc cttctccatc gagccgggca tctatttccc ggggcggtgg ggcgctcgca tcgaggacat cgtggtcgtg acggacgacg gcgccctggc tgtcaacaag cggccgcacg aactgatcgt ggtgccggtg ccgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MypaA.01407.a.A1.PW29502

PURIFICATION DATe:	8/23/2010
CONCENTRATION:	33.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

MESHRFDADV YARRLAAAAS ATAAAGLGGL VITPGYDLRY LVGSRAQTLE RFTALVLPAS GEPTVVVPRL EVASLKGSAI AELGLPVRDW VDGENPYELV AAALGGAPAA TAVTDSMPAL HLLPLAGVLG VLPVLATDVL RGLRMVKEEC EVDALRKAGA AIDRVHARVP SFLIPGRTEA DVAADIAEAI VAEGHSEVAF IIVGSGPHGA DPHHGYSDRE LQVGDIVVVD IGGSYEPGYH SDSTRTYSIG EPSPEVAQQY SILQRAQRAA CDAVRPGVTA EQVAAAARDV LAAAGLAEYF VHRTGHGIGL SVXEEPYIVA GNDLPLAAGM AFSIEPGIYF PGRWGARIED IVVVTDDGAL AVNKRPHELI VVPVP

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATCCCAC CGTTTCGACG CGGACGTGTA TGCGCGCCGG CTGGCGGCGG CCGCCTCGGC GACCGCCGCC GCCGGGCTGG GCGGCCTGGT GATCACGCCG GGGTATGACC TGCGCTACCT GGTCGGGTCG CGGGCGCAGA CCTTGGAGCG GTTCACCGCC CTGGTGTTGC CCGCCTCCGG TGAGCCGACC GTCGTGGTGC CGCGGCTGGA GGTGGCCTCG CTGAAGGGGT CGGCGATCGC CGAACTCGGT CTGCCGGTGC GGGATTGGGT CGACGGTGAG AACCCCTACG AACTGGTGGC CGCGGCGTTG GGCGGGGCGC CCGCCGCGAC CGCCGTCACC GACTCCATGC CGGCGCTGCA TCTGCTGCCG CTGGCCGGCG TGCTCGGCGT GCTGCCGGTG CTGGCCACCG ACGTGTTGCG CGGCCTGCGG ATGGTCAAGG AAGAGTGCGA GGTCGACGCC TTGCGGAAGG CTGGCGCGGC CATCGACCGG GTGCACGCCC GGGTGCCGTC GTTTCTGATA CCGGGCCGCA CCGAGGCTGA CGTGGCCGCC GATATCGCCG AAGCCATTGT GGCCGAAGGG CATTCGGAGG TGGCCTTCAT CATCGTCGGC TCCGGCCCGC ACGGCGCCGA CCCGCACCAC GGGTATTCGG ATCGCGAGCT GCAGGTGGGA GACATCGTCG TCGTCGACAT CGGGGGCTCC TACGAGCCGG GCTATCATTC GGATTCGACC CGCACCTACA GCATCGGGGA GCCGAGTCCC GAAGTGGCCC AACAGTATTC GATTCTGCAG CGCGCTCAGC GGGCGGCCTG TGATGCGGTC CGGCCCGGTG TGACGGCCGA GCAGGTCGCT GCTGCCGCCC GTGACGTGCT GGCCGCCGCG GGACTCGCGG AGTATTTCGT GCACCGCACG GGGCACGGCA TCGGGCTGTC GGTGCACGAA GAGCCTTACA TCGTTGCGGG AAACGACCTG CCGCTCGCCG CGGGCATGGC CTTCTCCATC GAGCCGGGCA TCTATTTCCC GGGGCGGTGG GGCGCTCGCA TCGAGGACAT CGTGGTCGTG ACGGACGACG GCGCCCTGGC TGTCAACAAG CGGCCGCACG AACTGATCGT GGTGCCGGTG CCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA