MysmA.01407.b

Xaa-Pro aminopeptidase

CENTER ID: MysmA.01407.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01407.b.A1.PS00665 Full length( MysmA.01407.b ) 1 388
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_177062
PATRIC ID: fig|246196.19.peg.2459
RefSeq: YP_886833.1
UniProt: A0QV95
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIADPAEYAG RLQRVHERMR TQSLSALVVC DPANIFYLTG YAAWSFYTPQ CLVVPADGDP HLFLRAMDAA GAASLCNVAA ERIHGYPEDL VHRPDTHPFR WIAAAVRSLI PAGEPVGVEG DANYFSPRAY FALAEGLPAN RLVDAGELVN WVRLVKSPYE IGQLQIAGAI AEHAMRTACE RIEPGRRQCD LVADILTAQT IGVDGHGGDF PAIVPMLPTG DAAGTPHCTW TDQPFLDGEA TTIELAGVHG RYHAPLARTV MLGEPPQRLA ETAKITAEAM QATLEAIRPG ATGASVHRAF DDVIRPHGLR KDSRLGYSIG IGFPPDWGER TVSLRREDTA ELAAGMAFHV ILGMWMDGWG YELSEPIVVT PTGFERLTNL PHELTIRR
NT Sequence
ATGATCGCGG ACCCGGCTGA GTACGCGGGT CGCCTACAGC GCGTGCACGA GCGCATGCGG ACACAGTCAC TGTCTGCGCT CGTGGTGTGC GACCCGGCCA ACATCTTCTA CCTCACCGGC TACGCCGCGT GGTCCTTCTA CACCCCGCAG TGCCTGGTGG TCCCCGCCGA CGGCGATCCG CACCTGTTCC TGCGGGCGAT GGATGCCGCC GGTGCGGCGT CGCTGTGCAA TGTCGCGGCC GAGCGCATCC ACGGCTACCC CGAGGATCTG GTGCACCGGC CGGACACCCA CCCGTTCAGG TGGATCGCCG CGGCGGTCAG GTCGCTGATC CCCGCCGGTG AGCCGGTGGG CGTCGAAGGC GACGCCAACT ACTTCTCCCC GCGCGCGTAC TTCGCGCTCG CAGAAGGCCT TCCGGCGAAC CGTCTGGTCG ACGCCGGGGA ACTGGTCAAC TGGGTCCGGC TGGTCAAGTC CCCCTACGAG ATCGGTCAAC TGCAGATCGC CGGTGCCATC GCCGAACACG CCATGCGGAC CGCGTGTGAA CGAATCGAGC CGGGCCGCAG GCAATGCGAC CTCGTCGCCG ACATCCTCAC CGCACAGACC ATCGGCGTGG ACGGGCACGG CGGCGACTTC CCGGCGATCG TGCCGATGCT TCCGACCGGT GACGCGGCCG GGACGCCCCA CTGCACCTGG ACCGACCAGC CGTTCCTCGA CGGCGAGGCC ACCACGATCG AACTGGCCGG GGTCCACGGC CGTTACCACG CACCCCTTGC CCGGACCGTC ATGCTCGGTG AGCCGCCGCA ACGCCTGGCC GAGACCGCGA AGATCACCGC CGAGGCCATG CAGGCGACGC TCGAGGCGAT CCGGCCCGGC GCCACGGGCG CTTCCGTACA CCGCGCGTTC GACGACGTGA TCCGGCCGCA CGGGCTGCGG AAAGATTCTC GGCTCGGTTA CTCGATCGGC ATCGGGTTCC CACCCGACTG GGGCGAGCGC ACGGTCAGCC TGCGTCGCGA GGACACCGCC GAACTCGCCG CGGGCATGGC GTTCCACGTC ATCCTCGGAA TGTGGATGGA CGGCTGGGGC TACGAACTGT CCGAGCCGAT CGTGGTCACC CCCACCGGGT TCGAGCGCCT GACGAACCTG CCACACGAGC TCACGATCAG GAGG
Details for MysmA.01407.b.A1.PS00665
PURIFICATION DATe: 6/22/2010
CONCENTRATION: 32.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MSXRXXXXYP EDLVXXPDTH PFRWIAAAVR SLIPAGEPVG VEGDANYFSP RAYFALAEGL PANRLVDAGE LVNWVRLVKS PYEIGQLQIA GAIAEHAMRT ACERIEPGRR QCDLVADILT AQTIGVDGHG GDFPAIVPML PTGDAAGTPH CTWTDQPFLD GEATTIELAG VHGRYHAPLA RTVMLGEPPQ RLAETAKITA EAMQATLEAI RPGATGASVH RAFDDVIRPH GLRKDSRLGY SIGIGFPPDW GERTVSLRRE DTAELAAGMA FHVILGMWMD GWGYELSEPI VVTPTGFERL TNLPHELTIR R
Validated NT Sequence
tnnttcnnnn tttgttagca gccggatcct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta cctcctgatc gtgagctcgt gtggcaggtt cgtcaggcgc tcgaacccgg tgggggtgac cacgatcggc tcggacagtt cgtagcccca gccgtccatc cacattccga ggatgacgtg gaacgccatg cccgcggcga gttcggcggt gtcctcgcga cgcaggctga ccgtgcgctc gccccagtcg ggtgggaacc cgatgccgat cgagtaaccg agccgagaat ctttccgcag cccgtgcggc cggatcacgt cgtcgaacgc gcggtgtacg gaagcgcccg tggcgccggg ccggatcgcc tcgagcgtcg cctgcatggc ctcggcggtg atcttcgcgg tctcggccag gcgttgcggc ggctcaccga gcatgacggt ccgggcaagg ggtgcgtggt aacggccgtg gaccccggcc agttcgatcg tggtggcctc gccgtcgagg aacggctggt cggtccaggt gcagtggggc gtcccggccg cgtcaccggt cggaagcatc ggcacgatcg ccgggaagtc gccgccgtgc ccgtccacgc cgatggtctg tgcggtgagg atgtcggcga cgaggtcgca ttgcctgcgg cccggctcga ttcgttcaca cgcggtccgc atggcgtgtt cggcgatggc accggcgatc tgcagttgac cgatctcgta gggggacttg accagccgga cccagttgac cagttccccg gcgtcgacca gacggttcgc cggaaggcct tctgcgagcg cgaagtacgc gcgcggggag aagtagttgg cgtcgccttc gacgcccacc ggctcaccgg cggggatcag cgacctgacc gccgcggcga tccacctgaa cgggtgggtg tccggncngn gcaccagatc ctcggggtan nngtnnngcn ctcgnnncga cattgcacag cgacgccgcn cngcggcatc ntcnccgcag nanngngcnn ntcnnntngn ngggnacacn nnnctgcggg gnnnancnnn cnnnnancnn nnnnnnnnnn nnnnnnngcn ggnnnnnnnn cnncnnnnnn nnnnnnnncn gnnnncnnna ngnnnnnnnn nnnnnnnnnn nnnnnncncn nnntnnnnnn nnannncnng ngnnnnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIADPAEYA GRLQRVHERM RTQSLSALVV CDPANIFYLT GYAAWSFYTP QCLVVPADGD PHLFLRAMDA AGAASLCNVA AERIHGYPED LVHRPDTHPF RWIAAAVRSL IPAGEPVGVE GDANYFSPRA YFALAEGLPA NRLVDAGELV NWVRLVKSPY EIGQLQIAGA IAEHAMRTAC ERIEPGRRQC DLVADILTAQ TIGVDGHGGD FPAIVPMLPT GDAAGTPHCT WTDQPFLDGE ATTIELAGVH GRYHAPLART VMLGEPPQRL AETAKITAEA MQATLEAIRP GATGASVHRA FDDVIRPHGL RKDSRLGYSI GIGFPPDWGE RTVSLRREDT AELAAGMAFH VILGMWMDGW GYELSEPIVV TPTGFERLTN LPHELTIRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCGCGGAC CCGGCTGAGT ACGCGGGTCG CCTACAGCGC GTGCACGAGC GCATGCGGAC ACAGTCACTG TCTGCGCTCG TGGTGTGCGA CCCGGCCAAC ATCTTCTACC TCACCGGCTA CGCCGCGTGG TCCTTCTACA CCCCGCAGTG CCTGGTGGTC CCCGCCGACG GCGATCCGCA CCTGTTCCTG CGGGCGATGG ATGCCGCCGG TGCGGCGTCG CTGTGCAATG TCGCGGCCGA GCGCATCCAC GGCTACCCCG AGGATCTGGT GCACCGGCCG GACACCCACC CGTTCAGGTG GATCGCCGCG GCGGTCAGGT CGCTGATCCC CGCCGGTGAG CCGGTGGGCG TCGAAGGCGA CGCCAACTAC TTCTCCCCGC GCGCGTACTT CGCGCTCGCA GAAGGCCTTC CGGCGAACCG TCTGGTCGAC GCCGGGGAAC TGGTCAACTG GGTCCGGCTG GTCAAGTCCC CCTACGAGAT CGGTCAACTG CAGATCGCCG GTGCCATCGC CGAACACGCC ATGCGGACCG CGTGTGAACG AATCGAGCCG GGCCGCAGGC AATGCGACCT CGTCGCCGAC ATCCTCACCG CACAGACCAT CGGCGTGGAC GGGCACGGCG GCGACTTCCC GGCGATCGTG CCGATGCTTC CGACCGGTGA CGCGGCCGGG ACGCCCCACT GCACCTGGAC CGACCAGCCG TTCCTCGACG GCGAGGCCAC CACGATCGAA CTGGCCGGGG TCCACGGCCG TTACCACGCA CCCCTTGCCC GGACCGTCAT GCTCGGTGAG CCGCCGCAAC GCCTGGCCGA GACCGCGAAG ATCACCGCCG AGGCCATGCA GGCGACGCTC GAGGCGATCC GGCCCGGCGC CACGGGCGCT TCCGTACACC GCGCGTTCGA CGACGTGATC CGGCCGCACG GGCTGCGGAA AGATTCTCGG CTCGGTTACT CGATCGGCAT CGGGTTCCCA CCCGACTGGG GCGAGCGCAC GGTCAGCCTG CGTCGCGAGG ACACCGCCGA ACTCGCCGCG GGCATGGCGT TCCACGTCAT CCTCGGAATG TGGATGGACG GCTGGGGCTA CGAACTGTCC GAGCCGATCG TGGTCACCCC CACCGGGTTC GAGCGCCTGA CGAACCTGCC ACACGAGCTC ACGATCAGGA GGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA