MyulA.01407.a
Dipeptidase PepE
| CENTER ID: | MyulA.01407.a |
| ORGANISM: | Mycobacterium ulcerans Agy99 |
| ASSOCIATED DISEASE: | Buruli, Bairnsdale ulcer |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.01407.a.A1.GE29890 | Full length( MyulA.01407.a ) | 1 | 375 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.01407.a.A1.PS00805 | Full length( MyulA.01407.a ) | 1 | 375 |
Structures
4EGE
DEPOSITED: 3/30/2012
DETERMINATION: XRay
CLONE: MyulA.01407.a.A1.GE29890
PROTEIN: MyulA.01407.a.A1.PS00805
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126673 |
| PATRIC ID: | fig|362242.7.peg.2737 |
| RefSeq: | YP_906163.1 |
| UniProt: | A0PQS3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSGRFDTAV YARRLAAAAA ATEQAGLAGL VITPGYDLRY LIGSRADTFE RLTALVLPAS GVPTIVLPRL ELASLKESAA SDLGVCVRDW VDGDDPYQLV AVALGGAPAA TAVTDSMPAL HLLPLADALG VLPVLATDVL RQLRMVKEAA EVDALAKAGA AIDRVHARVP AFLVPGRTEA QVAADIAEAI VAEGHSAVAF VIVGSGPHGA DPHHGYSDRK LQVGDIVVVD IGGTYEPGYY SDSTRTYSIG DPSPDVAQQY SALQRAQRAA VDAVRPGVTA AQVDAAARDV LADAGLAEYF VHRTGHGIGL CVHEEPYIVA GNELPLVAGM AFSIEPGIYF PGRWGARIED IVVVTENGAL SVNNRPHELM VVPVS
NT Sequence
ATGGATTCTG GGCGATTCGA CACCGCGGTG TACGCGCGAC GTTTGGCCGC GGCGGCGGCC GCGACCGAGC AGGCCGGCCT TGCCGGTCTG GTCATCACTC CGGGCTATGA CCTGCGCTAT CTCATCGGCT CGCGCGCCGA TACGTTCGAG CGGCTCACCG CGCTGGTGCT GCCCGCTTCT GGGGTGCCGA CCATCGTCTT GCCGCGCTTG GAGCTCGCGT CGCTCAAGGA GTCGGCCGCA TCGGATTTGG GAGTGTGCGT GCGCGATTGG GTCGACGGCG ATGACCCGTA CCAGCTGGTG GCCGTGGCGT TGGGGGGTGC GCCCGCAGCT ACCGCAGTTA CCGATTCGAT GCCGGCCCTG CACTTGTTGC CGCTGGCCGA TGCGCTGGGG GTACTGCCGG TATTGGCTAC CGATGTGCTG CGTCAGCTGC GGATGGTCAA GGAGGCGGCC GAGGTGGACG CGCTGGCCAA GGCCGGAGCC GCCATTGATC GGGTGCATGC CCGGGTACCG GCGTTTCTGG TGCCCGGCCG CACCGAGGCG CAGGTTGCCG CCGACATTGC CGAAGCGATT GTTGCCGAGG GGCATTCGGC AGTGGCATTC GTCATCGTCG GATCCGGTCC ACATGGCGCC GATCCGCACC ACGGATATTC GGACCGAAAG TTGCAGGTCG GCGACATCGT CGTGGTCGAT ATCGGGGGGA CATACGAGCC CGGATACTAC TCGGACTCGA CCCGAACCTA CAGCATCGGC GATCCTAGTC CTGATGTGGC ACAACAGTAT TCGGCCCTGC AGCGGGCTCA GCGTGCGGCC GTTGATGCGG TCCGTCCGGG GGTTACGGCG GCACAGGTCG ACGCTGCCGC CCGTGACGTG CTGGCCGACG CCGGATTGGC CGAGTACTTT GTGCACCGCA CCGGACACGG TATCGGGCTG TGTGTGCATG AGGAGCCCTA TATCGTCGCC GGCAACGAGT TGCCGCTGGT CGCGGGCATG GCGTTCTCGA TCGAGCCGGG CATCTACTTT CCGGGCCGGT GGGGAGCCCG CATCGAGGAC ATTGTGGTGG TTACCGAAAA CGGTGCGTTA TCGGTCAACA ATCGGCCGCA CGAGCTGATG GTGGTGCCGG TGTCT
Details for MyulA.01407.a.A1.GE29890
| HARVESTED ON: | 7/20/2010 |
| SEQUENCED ON: | 8/2/2010 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MDSGRFDTAV YARRLAAAAA ATEQAGLAGL VITPGYDLRY LIGSRADTFE RLTALVLPAS GVPTIVLPRL ELASLKESAA SDLGVCVRDW VDGDDPYQLV AVALGGAPAA TAVTDSMPAL HLLPLADALG VLPVLATDVL RQLRMVKEAA EVDALAKAGA AIDRVHARVP AFLVPGRTEA QVAADIAEAI VAEGHSAVAF VIVGSGPHGA DPHHGYSDRK LQVGDIVVVD IGGTYEPGYY SDSTRTYSIG DPSPDVAQQY SALQRAQRAA VDAVRPGVTA AQVDAAARDV LADAGLAEYF VHRTGHGIGL CVHEEPYIVA GNELPLVAGM AFSIEPGIYF PGRWGARIED IVVVTENGAL SVNNRPHELM VVP
Validated NT Sequence
caccggcacc accatcagct cgtgcggccg attgttgacc gataacgcac cgttttcggt aaccaccaca atgtcctcga tgcgggctcc ccaccggccc ggaaagtaga tgcccggctc gatcgagaac gccatgcccg cgaccagcgg caactcgttg ccggcgacga tatagggctc ctcatgcaca cacagcccga taccgtgtcc ggtgcggtgc acaaagtact cggccaatcc ggcgtcggcc agcacgtcac gggcggcagc gtcgacctgt gccgccgtaa cccccggacg gaccgcatca acggccgcac gctgagcccg ctgcagggcc gaatactgtt gtgccacatc aggactagga tcgccgatgc tgtaggttcg ggtcgagtcc gagtagtatc cgggctcgta tgtccccccg atatcgacca cgacgatgtc gccgacctgc aactttcggt ccgaatatcc gtggtgcgga tcggcgccat gtggaccgga tccgacgatg acgaatgcca ctgccgaatg cccctcggca acaatcgctt cggcaatgtc ggcggcaacc tgcgcctcgg tgcggccggg caccagaaac gccggtaccc gggcatgcac ccgatcaatg gcggctccgg ccttggccag cgcgtccacc tcggccgcct ccttgaccat ccgcagctga cgcagcacat cggtagccaa taccggcagt acccccagcg catcggccag cggcaacaag tgcagggccg gcatcgaatc ggtaactgcg gtagctgcgg gcgcaccccc caacgccacg gccaccagct ggtacgggtc atcgccgtcg acccaatcgc gcacgcacac tcccaaatcc gatgcggccg actccttgag cgacgcgagc tccaagcgcg gcaagacgat ggtcggcacc ccagaagcgg gcagcaccag cgcggtgagc cgctcgaacg tatcggcgcg cgagccgatg agatagcgca ggtcatagcc cggagtgatg accagaccgg caaggccggc ctgctcggtc gcggccgccg ccgcggccaa acgtcgcgcg tacaccgcgg tgtcgaatcg cccagaatcc atcgaaccag gaccctgggt ctgagcttcc agggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSGRFDTA VYARRLAAAA AATEQAGLAG LVITPGYDLR YLIGSRADTF ERLTALVLPA SGVPTIVLPR LELASLKESA ASDLGVCVRD WVDGDDPYQL VAVALGGAPA ATAVTDSMPA LHLLPLADAL GVLPVLATDV LRQLRMVKEA AEVDALAKAG AAIDRVHARV PAFLVPGRTE AQVAADIAEA IVAEGHSAVA FVIVGSGPHG ADPHHGYSDR KLQVGDIVVV DIGGTYEPGY YSDSTRTYSI GDPSPDVAQQ YSALQRAQRA AVDAVRPGVT AAQVDAAARD VLADAGLAEY FVHRTGHGIG LCVHEEPYIV AGNELPLVAG MAFSIEPGIY FPGRWGARIE DIVVVTENGA LSVNNRPHEL MVVPV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggattctggg cgattcgaca ccgcggtgta cgcgcgacgt ttggccgcgg cggcggccgc gaccgagcag gccggccttg ccggtctggt catcactccg ggctatgacc tgcgctatct catcggctcg cgcgccgata cgttcgagcg gctcaccgcg ctggtgctgc ccgcttctgg ggtgccgacc atcgtcttgc cgcgcttgga gctcgcgtcg ctcaaggagt cggccgcatc ggatttggga gtgtgcgtgc gcgattgggt cgacggcgat gacccgtacc agctggtggc cgtggcgttg gggggtgcgc ccgcagctac cgcagttacc gattcgatgc cggccctgca cttgttgccg ctggccgatg cgctgggggt actgccggta ttggctaccg atgtgctgcg tcagctgcgg atggtcaagg aggcggccga ggtggacgcg ctggccaagg ccggagccgc cattgatcgg gtgcatgccc gggtaccggc gtttctggtg cccggccgca ccgaggcgca ggttgccgcc gacattgccg aagcgattgt tgccgagggg cattcggcag tggcattcgt catcgtcgga tccggtccac atggcgccga tccgcaccac ggatattcgg accgaaagtt gcaggtcggc gacatcgtcg tggtcgatat cggggggaca tacgagcccg gatactactc ggactcgacc cgaacctaca gcatcggcga tcctagtcct gatgtggcac aacagtattc ggccctgcag cgggctcagc gtgcggccgt tgatgcggtc cgtccggggg ttacggcggc acaggtcgac gctgccgccc gtgacgtgct ggccgacgcc ggattggccg agtactttgt gcaccgcacc ggacacggta tcgggctgtg tgtgcatgag gagccctata tcgtcgccgg caacgagttg ccgctggtcg cgggcatggc gttctcgatc gagccgggca tctactttcc gggccggtgg ggagcccgca tcgaggacat tgtggtggtt accgaaaacg gtgcgttatc ggtcaacaat cggccgcacg agctgatggt ggtgccggtg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyulA.01407.a.A1.PS00805
| PURIFICATION DATe: | 10/14/2010 |
| CONCENTRATION: | 57.89mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDSGRFDTAV YARRLAAAAA ATEQAGLAGL VITPGYDLRY LIGSRADTFE RLTALVLPAS GVPTIVLPRL ELASLKESAA SDLGVCVRDW VDGDDPYQLV AVALGGAPAA TAVTDSMPAL HLLPLADALG VLPVLATDVL RQLRMVKEAA EVDALAKAGA AIDRVHARVP AFLVPGRTEA QVAADIAEAI VAEGHSAVAF VIVGSGPHGA DPHHGYSDRK LQVGDIVVVD IGGTYEPGYY SDSTRTYSIG DPSPDVAQQY SALQRAQRAA VDAVRPGVTA AQVDAAARDV LADAGLAEYF VHRTGHGIGL CVHEEPYIVA GNELPLVAGM AFSIEPGIYF PGRWGARIED IVVVTENGAL SVNNRPHELM VVP
Validated NT Sequence
caccggcacc accatcagct cgtgcggccg attgttgacc gataacgcac cgttttcggt aaccaccaca atgtcctcga tgcgggctcc ccaccggccc ggaaagtaga tgcccggctc gatcgagaac gccatgcccg cgaccagcgg caactcgttg ccggcgacga tatagggctc ctcatgcaca cacagcccga taccgtgtcc ggtgcggtgc acaaagtact cggccaatcc ggcgtcggcc agcacgtcac gggcggcagc gtcgacctgt gccgccgtaa cccccggacg gaccgcatca acggccgcac gctgagcccg ctgcagggcc gaatactgtt gtgccacatc aggactagga tcgccgatgc tgtaggttcg ggtcgagtcc gagtagtatc cgggctcgta tgtccccccg atatcgacca cgacgatgtc gccgacctgc aactttcggt ccgaatatcc gtggtgcgga tcggcgccat gtggaccgga tccgacgatg acgaatgcca ctgccgaatg cccctcggca acaatcgctt cggcaatgtc ggcggcaacc tgcgcctcgg tgcggccggg caccagaaac gccggtaccc gggcatgcac ccgatcaatg gcggctccgg ccttggccag cgcgtccacc tcggccgcct ccttgaccat ccgcagctga cgcagcacat cggtagccaa taccggcagt acccccagcg catcggccag cggcaacaag tgcagggccg gcatcgaatc ggtaactgcg gtagctgcgg gcgcaccccc caacgccacg gccaccagct ggtacgggtc atcgccgtcg acccaatcgc gcacgcacac tcccaaatcc gatgcggccg actccttgag cgacgcgagc tccaagcgcg gcaagacgat ggtcggcacc ccagaagcgg gcagcaccag cgcggtgagc cgctcgaacg tatcggcgcg cgagccgatg agatagcgca ggtcatagcc cggagtgatg accagaccgg caaggccggc ctgctcggtc gcggccgccg ccgcggccaa acgtcgcgcg tacaccgcgg tgtcgaatcg cccagaatcc atcgaaccag gaccctgggt ctgagcttcc agggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSGRFDTA VYARRLAAAA AATEQAGLAG LVITPGYDLR YLIGSRADTF ERLTALVLPA SGVPTIVLPR LELASLKESA ASDLGVCVRD WVDGDDPYQL VAVALGGAPA ATAVTDSMPA LHLLPLADAL GVLPVLATDV LRQLRMVKEA AEVDALAKAG AAIDRVHARV PAFLVPGRTE AQVAADIAEA IVAEGHSAVA FVIVGSGPHG ADPHHGYSDR KLQVGDIVVV DIGGTYEPGY YSDSTRTYSI GDPSPDVAQQ YSALQRAQRA AVDAVRPGVT AAQVDAAARD VLADAGLAEY FVHRTGHGIG LCVHEEPYIV AGNELPLVAG MAFSIEPGIY FPGRWGARIE DIVVVTENGA LSVNNRPHEL MVVPV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATTCTGGG CGATTCGACA CCGCGGTGTA CGCGCGACGT TTGGCCGCGG CGGCGGCCGC GACCGAGCAG GCCGGCCTTG CCGGTCTGGT CATCACTCCG GGCTATGACC TGCGCTATCT CATCGGCTCG CGCGCCGATA CGTTCGAGCG GCTCACCGCG CTGGTGCTGC CCGCTTCTGG GGTGCCGACC ATCGTCTTGC CGCGCTTGGA GCTCGCGTCG CTCAAGGAGT CGGCCGCATC GGATTTGGGA GTGTGCGTGC GCGATTGGGT CGACGGCGAT GACCCGTACC AGCTGGTGGC CGTGGCGTTG GGGGGTGCGC CCGCAGCTAC CGCAGTTACC GATTCGATGC CGGCCCTGCA CTTGTTGCCG CTGGCCGATG CGCTGGGGGT ACTGCCGGTA TTGGCTACCG ATGTGCTGCG TCAGCTGCGG ATGGTCAAGG AGGCGGCCGA GGTGGACGCG CTGGCCAAGG CCGGAGCCGC CATTGATCGG GTGCATGCCC GGGTACCGGC GTTTCTGGTG CCCGGCCGCA CCGAGGCGCA GGTTGCCGCC GACATTGCCG AAGCGATTGT TGCCGAGGGG CATTCGGCAG TGGCATTCGT CATCGTCGGA TCCGGTCCAC ATGGCGCCGA TCCGCACCAC GGATATTCGG ACCGAAAGTT GCAGGTCGGC GACATCGTCG TGGTCGATAT CGGGGGGACA TACGAGCCCG GATACTACTC GGACTCGACC CGAACCTACA GCATCGGCGA TCCTAGTCCT GATGTGGCAC AACAGTATTC GGCCCTGCAG CGGGCTCAGC GTGCGGCCGT TGATGCGGTC CGTCCGGGGG TTACGGCGGC ACAGGTCGAC GCTGCCGCCC GTGACGTGCT GGCCGACGCC GGATTGGCCG AGTACTTTGT GCACCGCACC GGACACGGTA TCGGGCTGTG TGTGCATGAG GAGCCCTATA TCGTCGCCGG CAACGAGTTG CCGCTGGTCG CGGGCATGGC GTTCTCGATC GAGCCGGGCA TCTACTTTCC GGGCCGGTGG GGAGCCCGCA TCGAGGACAT TGTGGTGGTT ACCGAAAACG GTGCGTTATC GGTCAACAAT CGGCCGCACG AGCTGATGGT GGTGCCGGTG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA