MyabA.01407.a

Probable dipeptidase PepE

CENTER ID: MyabA.01407.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01407.a.A1.GE30511 Full length( MyabA.01407.a ) 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01407.a.A1.PS00919 Full length( MyabA.01407.a ) 1 374

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126673
PATRIC ID: fig|36809.5.peg.2231
RefSeq: YP_001702927.1
UniProt: B1MAK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPQRFESDV YADRLRRAAQ EAEKVGVTGL VVTPGYDLRY LTGSRAQTFE RLTALVVPAT GQPTVVAPRM ELAALRDSAI GDLNIPISDW VDGENPYELV RAALGTAGTV AVTESMPALH LLPLAQLVGT VPVLATGVLR RLRMVKDASE IDALRKAGAA IDRVHALVPE LLIPGRTEAQ VAADIAEAIV AEGHSEVAFI IVGSGPHGAD PHHECSDREL RTGDIVVVDI GGSYEPGYNS DSTRTYSIGE PDPDVAQRIS VLERAQQAAV HAARPGVTAE SVDAAARRVL TEAGMGEAFV HRTGHGIGLS VHEEPYIVEG NELVLEPGMA FSIEPGVYFP GQWGARIEDI VVITENGCES VNSRPHGLTV VPTR
NT Sequence
ATGGCACCCC AGCGTTTCGA ATCCGACGTC TACGCCGACC GACTGCGACG CGCGGCCCAG GAGGCCGAGA AGGTGGGAGT GACGGGTCTG GTGGTCACCC CCGGATACGA CCTGCGGTAC CTCACGGGAT CGCGCGCGCA GACGTTCGAG CGGCTCACCG CCCTGGTGGT GCCGGCGACG GGGCAGCCCA CTGTGGTCGC TCCTCGGATG GAGCTTGCGG CGCTGCGGGA TTCAGCTATC GGCGACCTGA ACATTCCCAT CAGCGATTGG GTCGACGGGG AGAATCCCTA CGAGCTGGTG CGCGCGGCAC TGGGGACCGC CGGAACTGTG GCGGTGACCG AGTCGATGCC CGCACTGCAC CTGTTGCCGC TGGCGCAACT GGTCGGAACG GTGCCGGTCT TGGCAACCGG CGTGCTACGC CGGTTGCGCA TGGTCAAAGA CGCCTCCGAG ATCGACGCGC TGCGTAAGGC CGGCGCCGCC ATCGACCGGG TGCACGCCCT GGTACCCGAA TTACTGATTC CGGGGCGCAC GGAGGCGCAG GTGGCCGCGG ATATCGCTGA AGCGATTGTG GCCGAGGGGC ATTCGGAGGT GGCTTTCATC ATCGTCGGTT CCGGCCCACA CGGGGCCGAC CCGCACCATG AGTGCTCGGA TCGTGAACTG CGGACCGGGG ACATTGTGGT GGTGGACATC GGCGGTAGCT ACGAGCCGGG TTATAACTCC GACAGCACCC GCACCTACAG TATTGGTGAG CCTGACCCCG ATGTTGCTCA ACGGATTTCG GTGCTTGAGC GGGCGCAGCA GGCGGCGGTA CATGCGGCCC GTCCCGGTGT GACGGCGGAG TCGGTGGATG CCGCCGCGCG CCGGGTTCTG ACCGAAGCGG GGATGGGTGA GGCCTTTGTA CATCGCACCG GACACGGCAT CGGGCTGTCG GTACATGAGG AGCCGTACAT CGTCGAGGGC AACGAACTGG TACTCGAACC CGGTATGGCC TTCAGTATTG AGCCGGGCGT GTACTTCCCG GGCCAGTGGG GTGCGCGGAT CGAGGACATC GTCGTCATCA CCGAAAACGG TTGCGAATCG GTGAATTCGC GGCCCCACGG CCTGACGGTG GTGCCTACTC GC
Details for MyabA.01407.a.A1.GE30511
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAPQRFESDV YADRLRRAAQ EAEKVGVTGL VVTPGYDLRY LTGSRAQTFE RLTALVVPAT GQPTVVAPRM ELAALRDSAI GDLNIPISDW VDGENPYELV RAALGTAGTV AVTESMPALH LLPLAQLVGT VPVLATGVLR RLRMVKDASE IDALRKAGAA IDRVHALVPE LLIPGRTEAQ VAADIAEAIV AEGHSEVAFI IVGSGPHGAD PHHECSDREL RTGDIVVVDI GGSYEPGYNS DSTRTYSIGE PDPDVAQRIS VLERAQQAAV HAARPGVTAE SVDAAARRVL TEAGMGEAFV HRTGHGIGLS VHEEPYIVEG NELVLEPGMA FSIEPGVYFP GQWGARIEDI VVITENGCES VNSRPHGLTV VPTR
Validated NT Sequence
ttattagcga gtaggcacca ccgtcaggcc gtggggccgc gaattcaccg attcgcaacc gttttcggtg atgacgacga tgtcctcgat ccgcgcaccc cactggcccg ggaagtacac gcccggctca atactgaagg ccataccggg ttcgagtacc agttcgttgc cctcgacgat gtacggctcc tcatgtaccg acagcccgat gccgtgtccg gtgcgatgta caaaggcctc acccatcccc gcttcggtca gaacccggcg cgcggcggca tccaccgact ccgccgtcac accgggacgg gccgcatgta ccgccgcctg ctgcgcccgc tcaagcaccg aaatccgttg agcaacatcg gggtcaggct caccaatact gtaggtgcgg gtgctgtcgg agttataacc cggctcgtag ctaccgccga tgtccaccac cacaatgtcc ccggtccgca gttcacgatc cgagcactca tggtgcgggt cggccccgtg tgggccggaa ccgacgatga tgaaagccac ctccgaatgc ccctcggcca caatcgcttc agcgatatcc gcggccacct gcgcctccgt gcgccccgga atcagtaatt cgggtaccag ggcgtgcacc cggtcgatgg cggcgccggc cttacgcagc gcgtcgatct cggaggcgtc tttgaccatg cgcaaccggc gtagcacgcc ggttgccaag accggcaccg ttccgaccag ttgcgccagc ggcaacaggt gcagtgcggg catcgactcg gtcaccgcca cagttccggc ggtccccagt gccgcgcgca ccagctcgta gggattctcc ccgtcgaccc aatcgctgat gggaatgttc aggtcgccga tagctgaatc ccgcagcgcc gcaagctcca tccgaggagc gaccacagtg ggctgccccg tcgccggcac caccagggcg gtgagccgct cgaacgtctg cgcgcgcgat cccgtgaggt accgcaggtc gtatccgggg gtgaccacca gacccgtcac tcccaccttc tcggcctcct gggccgcgcg tcgcagtcgg tcggcgtaga cgtcggattc gaaacgctgg ggtgccatcg aaccaggacc ctgggtctga gcttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPQRFESD VYADRLRRAA QEAEKVGVTG LVVTPGYDLR YLTGSRAQTF ERLTALVVPA TGQPTVVAPR MELAALRDSA IGDLNIPISD WVDGENPYEL VRAALGTAGT VAVTESMPAL HLLPLAQLVG TVPVLATGVL RRLRMVKDAS EIDALRKAGA AIDRVHALVP ELLIPGRTEA QVAADIAEAI VAEGHSEVAF IIVGSGPHGA DPHHECSDRE LRTGDIVVVD IGGSYEPGYN SDSTRTYSIG EPDPDVAQRI SVLERAQQAA VHAARPGVTA ESVDAAARRV LTEAGMGEAF VHRTGHGIGL SVHEEPYIVE GNELVLEPGM AFSIEPGVYF PGQWGARIED IVVITENGCE SVNSRPHGLT VVPTR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaccccag cgtttcgaat ccgacgtcta cgccgaccga ctgcgacgcg cggcccagga ggccgagaag gtgggagtga cgggtctggt ggtcaccccc ggatacgacc tgcggtacct cacgggatcg cgcgcgcaga cgttcgagcg gctcaccgcc ctggtggtgc cggcgacggg gcagcccact gtggtcgctc ctcggatgga gcttgcggcg ctgcgggatt cagctatcgg cgacctgaac attcccatca gcgattgggt cgacggggag aatccctacg agctggtgcg cgcggcactg gggaccgccg gaactgtggc ggtgaccgag tcgatgcccg cactgcacct gttgccgctg gcgcaactgg tcggaacggt gccggtcttg gcaaccggcg tgctacgccg gttgcgcatg gtcaaagacg cctccgagat cgacgcgctg cgtaaggccg gcgccgccat cgaccgggtg cacgccctgg tacccgaatt actgattccg gggcgcacgg aggcgcaggt ggccgcggat atcgctgaag cgattgtggc cgaggggcat tcggaggtgg ctttcatcat cgtcggttcc ggcccacacg gggccgaccc gcaccatgag tgctcggatc gtgaactgcg gaccggggac attgtggtgg tggacatcgg cggtagctac gagccgggtt ataactccga cagcacccgc acctacagta ttggtgagcc tgaccccgat gttgctcaac ggatttcggt gcttgagcgg gcgcagcagg cggcggtaca tgcggcccgt cccggtgtga cggcggagtc ggtggatgcc gccgcgcgcc gggttctgac cgaagcgggg atgggtgagg cctttgtaca tcgcaccgga cacggcatcg ggctgtcggt acatgaggag ccgtacatcg tcgagggcaa cgaactggta ctcgaacccg gtatggcctt cagtattgag ccgggcgtgt acttcccggg ccagtggggt gcgcggatcg aggacatcgt cgtcatcacc gaaaacggtt gcgaatcggt gaattcgcgg ccccacggcc tgacggtggt gcctactcgc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.01407.a.A1.PS00919
PURIFICATION DATe: 12/16/2010
CONCENTRATION: 100.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MAPQRFESDV YADRLRRAAQ EAEKVGVTGL VVTPGYDLRY LTGSRAQTFE RLTALVVPAT GQPTVVAPRM ELAALRDSAI GDLNIPISDW VDGENPYELV RAALGTAGTV AVTESMPALH LLPLAQLVGT VPVLATGVLR RLRMVKDASE IDALRKAGAA IDRVHALVPE LLIPGRTEAQ VAADIAEAIV AEGHSEVAFI IVGSGPHGAD PHHECSDREL RTGDIVVVDI GGSYEPGYNS DSTRTYSIGE PDPDVAQRIS VLERAQQAAV HAARPGVTAE SVDAAARRVL TEAGMGEAFV HRTGHGIGLS VHEEPYIVEG NELVLEPGMA FSIEPGVYFP GQWGARIEDI VVITENGCES VNSRPHGLTV VPTR
Validated NT Sequence
ttattagcga gtaggcacca ccgtcaggcc gtggggccgc gaattcaccg attcgcaacc gttttcggtg atgacgacga tgtcctcgat ccgcgcaccc cactggcccg ggaagtacac gcccggctca atactgaagg ccataccggg ttcgagtacc agttcgttgc cctcgacgat gtacggctcc tcatgtaccg acagcccgat gccgtgtccg gtgcgatgta caaaggcctc acccatcccc gcttcggtca gaacccggcg cgcggcggca tccaccgact ccgccgtcac accgggacgg gccgcatgta ccgccgcctg ctgcgcccgc tcaagcaccg aaatccgttg agcaacatcg gggtcaggct caccaatact gtaggtgcgg gtgctgtcgg agttataacc cggctcgtag ctaccgccga tgtccaccac cacaatgtcc ccggtccgca gttcacgatc cgagcactca tggtgcgggt cggccccgtg tgggccggaa ccgacgatga tgaaagccac ctccgaatgc ccctcggcca caatcgcttc agcgatatcc gcggccacct gcgcctccgt gcgccccgga atcagtaatt cgggtaccag ggcgtgcacc cggtcgatgg cggcgccggc cttacgcagc gcgtcgatct cggaggcgtc tttgaccatg cgcaaccggc gtagcacgcc ggttgccaag accggcaccg ttccgaccag ttgcgccagc ggcaacaggt gcagtgcggg catcgactcg gtcaccgcca cagttccggc ggtccccagt gccgcgcgca ccagctcgta gggattctcc ccgtcgaccc aatcgctgat gggaatgttc aggtcgccga tagctgaatc ccgcagcgcc gcaagctcca tccgaggagc gaccacagtg ggctgccccg tcgccggcac caccagggcg gtgagccgct cgaacgtctg cgcgcgcgat cccgtgaggt accgcaggtc gtatccgggg gtgaccacca gacccgtcac tcccaccttc tcggcctcct gggccgcgcg tcgcagtcgg tcggcgtaga cgtcggattc gaaacgctgg ggtgccatcg aaccaggacc ctgggtctga gcttccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPQRFESD VYADRLRRAA QEAEKVGVTG LVVTPGYDLR YLTGSRAQTF ERLTALVVPA TGQPTVVAPR MELAALRDSA IGDLNIPISD WVDGENPYEL VRAALGTAGT VAVTESMPAL HLLPLAQLVG TVPVLATGVL RRLRMVKDAS EIDALRKAGA AIDRVHALVP ELLIPGRTEA QVAADIAEAI VAEGHSEVAF IIVGSGPHGA DPHHECSDRE LRTGDIVVVD IGGSYEPGYN SDSTRTYSIG EPDPDVAQRI SVLERAQQAA VHAARPGVTA ESVDAAARRV LTEAGMGEAF VHRTGHGIGL SVHEEPYIVE GNELVLEPGM AFSIEPGVYF PGQWGARIED IVVITENGCE SVNSRPHGLT VVPTR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACCCCAG CGTTTCGAAT CCGACGTCTA CGCCGACCGA CTGCGACGCG CGGCCCAGGA GGCCGAGAAG GTGGGAGTGA CGGGTCTGGT GGTCACCCCC GGATACGACC TGCGGTACCT CACGGGATCG CGCGCGCAGA CGTTCGAGCG GCTCACCGCC CTGGTGGTGC CGGCGACGGG GCAGCCCACT GTGGTCGCTC CTCGGATGGA GCTTGCGGCG CTGCGGGATT CAGCTATCGG CGACCTGAAC ATTCCCATCA GCGATTGGGT CGACGGGGAG AATCCCTACG AGCTGGTGCG CGCGGCACTG GGGACCGCCG GAACTGTGGC GGTGACCGAG TCGATGCCCG CACTGCACCT GTTGCCGCTG GCGCAACTGG TCGGAACGGT GCCGGTCTTG GCAACCGGCG TGCTACGCCG GTTGCGCATG GTCAAAGACG CCTCCGAGAT CGACGCGCTG CGTAAGGCCG GCGCCGCCAT CGACCGGGTG CACGCCCTGG TACCCGAATT ACTGATTCCG GGGCGCACGG AGGCGCAGGT GGCCGCGGAT ATCGCTGAAG CGATTGTGGC CGAGGGGCAT TCGGAGGTGG CTTTCATCAT CGTCGGTTCC GGCCCACACG GGGCCGACCC GCACCATGAG TGCTCGGATC GTGAACTGCG GACCGGGGAC ATTGTGGTGG TGGACATCGG CGGTAGCTAC GAGCCGGGTT ATAACTCCGA CAGCACCCGC ACCTACAGTA TTGGTGAGCC TGACCCCGAT GTTGCTCAAC GGATTTCGGT GCTTGAGCGG GCGCAGCAGG CGGCGGTACA TGCGGCCCGT CCCGGTGTGA CGGCGGAGTC GGTGGATGCC GCCGCGCGCC GGGTTCTGAC CGAAGCGGGG ATGGGTGAGG CCTTTGTACA TCGCACCGGA CACGGCATCG GGCTGTCGGT ACATGAGGAG CCGTACATCG TCGAGGGCAA CGAACTGGTA CTCGAACCCG GTATGGCCTT CAGTATTGAG CCGGGCGTGT ACTTCCCGGG CCAGTGGGGT GCGCGGATCG AGGACATCGT CGTCATCACC GAAAACGGTT GCGAATCGGT GAATTCGCGG CCCCACGGCC TGACGGTGGT GCCTACTCGC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA