SSGCID: Target Page

MytuD.01152.a

uroporphyrinogen decarboxylase

CENTER ID:	MytuD.01152.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01152.a.A1.GE30668	Full length( MytuD.01152.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2988
RefSeq:	NP_217194.1
TubercuList:	Rv2678c
MTB Network Portal:	Rv2678c
UniProt:	P9WFE1

Orthologues for Rv2678c

Species	% Identity	% Coverage	Clones	Proteins
marinum	86	100	1	0
ulcerans	85	100	1	0
leprae	84	100	1	0
avium	78	99	1	0
paratuberculosis	78	99	1	0
smegmatis	73	100	1	1
abscessus	70	100	1	1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTRRDLPQS PYLAAVTGRK PSRVPVWFMR QAGRSLPEYR ALRERYSMLA ACFEPDVACE ITLQPIRRYD VDAAILFSDI VVPLRAAGVD LDIVADVGPV IADPVRTAAD VAAMKPLDPQ AIQPVLVAAS LLVAELGDVP LIGFAGAPFT LASYLVEGGP SRHHAHVKAM MLAEPASWHA LMAKLTDLTI AFLVGQIDAG VDAIQVFDSW AGALSPIDYR QYVLPHSARV FAALGEHGVP MTHFGVGTAE LLGAMSEAVT AGERPGRGAV VGVDWRTPLT DAAARVVPGT ALQGNLDPAV VLAGWPAVER AARAVVDDGR RAVDAGAAGH IFNLGHGVLP ESDPAVLADL VSLVHSL

NT Sequence

ATGAGTACCC GTCGCGACCT TCCGCAGTCG CCGTATCTGG CCGCCGTCAC CGGCCGCAAG CCCAGCCGGG TGCCGGTGTG GTTCATGCGG CAGGCCGGGC GCTCGTTGCC CGAATACCGG GCGCTGCGCG AGCGGTACAG CATGCTGGCG GCCTGTTTCG AACCGGACGT GGCCTGTGAG ATTACCCTGC AGCCGATACG CCGCTACGAC GTCGACGCGG CCATCCTGTT CTCCGACATC GTGGTGCCGC TACGGGCGGC CGGCGTGGAT TTGGACATCG TCGCCGACGT TGGGCCGGTG ATCGCGGACC CGGTGCGCAC CGCCGCCGAT GTCGCCGCCA TGAAACCACT TGATCCACAA GCGATTCAAC CGGTATTGGT GGCGGCTTCG TTGTTGGTCG CTGAGCTTGG CGACGTGCCG CTGATCGGTT TCGCCGGCGC GCCGTTCACG CTGGCCTCCT ACCTCGTGGA GGGCGGACCG AGCCGCCATC ACGCCCACGT CAAGGCGATG ATGTTGGCCG AGCCGGCGAG CTGGCACGCG CTGATGGCCA AGCTGACCGA CCTCACCATC GCGTTTCTGG TCGGCCAAAT CGACGCGGGG GTGGACGCCA TCCAGGTATT CGACTCCTGG GCCGGGGCGC TCTCGCCGAT CGACTACCGC CAGTACGTGT TGCCGCACAG CGCCCGGGTG TTCGCTGCGC TGGGCGAGCA TGGCGTGCCG ATGACGCACT TCGGGGTTGG GACCGCCGAA TTGCTGGGCG CGATGTCGGA GGCCGTGACA GCCGGGGAGC GGCCAGGCCG AGGGGCCGTC GTCGGCGTGG ACTGGCGGAC CCCGCTGACC GACGCCGCCG CCCGGGTGGT GCCCGGCACG GCGTTACAGG GCAACCTCGA TCCGGCGGTG GTCCTGGCGG GCTGGCCGGC GGTGGAACGT GCCGCACGTG CCGTTGTCGA CGACGGACGC CGCGCCGTCG ACGCCGGTGC GGCCGGCCAT ATCTTCAACC TCGGCCACGG GGTGCTGCCA GAAAGCGATC CCGCGGTGCT GGCCGACCTG GTCTCGCTCG TACACTCGCT A

Details for MytuD.01152.a.A1.GE30668

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

accctggaag ctcagaccca gggtcctggt tcgatgagta cccgtcgcga ccttccgcag tcgccgtatc tggccgccgt caccggccgc aagcccagcc gggtgccggt gtggttcatg cggcaggccg ggcgctcgtt gcccgaatac cgggcgctgc gcgagcggta cagcatgctg gcggcctgtt tcgaaccgga cgtggcctgt gagattaccc tgcagccgat acgccgctac gacgtcgacg cggccatcct gttctccgac atcgtggtgc cgctacgggc ggccggcgtg gatttggaca tcgtcgccga cgttgggccg gtgatcgcgg acccggtgcg caccgccgcc gatgtcgccg ccatgaaacc acttgatcca caagcgattc aaccggtatt ggtggcggct tcgttgttgg tcgctgagct tggcgacgtg ccgctgatcg gtttcgccgg cgcgccgttc acgctggcct cctacctcgt ggagggcgga ccgagccgcc atcacgccca cgtcaaggcg atgatgttgg ccgagccggc gagctggcac gcgctgatgg ccaagctgac cgacctcacc atcgcgtttc tggtcggcca aatcgacgcg ggggtggacg ccatccaggt attcgactcc tgggccgggg cgctctcgcc gatcgactac cgccagtacg tgttgccgca cagcgcccgg gtgttcgctg cgctgggcga gcatggcgtg ccgatgacgc acttcggggt tgggaccgcc gaattgctgg gcgcgatgtc ggaggccgtg acagccgggg agcggccagg ccgaggggcc gtcgtcggcg tggactggcg gaccccgctg accgacgccg ccgcccgggt ggtgcccggc acggcgttac agggcaacct cgatccggcg gtggtcctgg cgggctggcc ggcggtggaa cgtgccgcac gtgccgttgt cgacgacgga cgccgcgccg tcgacgccgg tgcggccggc catatcttca acctcggcca cggggtgctg ccagaaagcg atcccgcggt gctggccgac ctggtctcgc tcgtacactc gctataata

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTRRDLPQ SPYLAAVTGR KPSRVPVWFM RQAGRSLPEY RALRERYSML AACFEPDVAC EITLQPIRRY DVDAAILFSD IVVPLRAAGV DLDIVADVGP VIADPVRTAA DVAAMKPLDP QAIQPVLVAA SLLVAELGDV PLIGFAGAPF TLASYLVEGG PSRHHAHVKA MMLAEPASWH ALMAKLTDLT IAFLVGQIDA GVDAIQVFDS WAGALSPIDY RQYVLPHSAR VFAALGEHGV PMTHFGVGTA ELLGAMSEAV TAGERPGRGA VVGVDWRTPL TDAAARVVPG TALQGNLDPA VVLAGWPAVE RAARAVVDDG RRAVDAGAAG HIFNLGHGVL PESDPAVLAD LVSLVHSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtacccgt cgcgaccttc cgcagtcgcc gtatctggcc gccgtcaccg gccgcaagcc cagccgggtg ccggtgtggt tcatgcggca ggccgggcgc tcgttgcccg aataccgggc gctgcgcgag cggtacagca tgctggcggc ctgtttcgaa ccggacgtgg cctgtgagat taccctgcag ccgatacgcc gctacgacgt cgacgcggcc atcctgttct ccgacatcgt ggtgccgcta cgggcggccg gcgtggattt ggacatcgtc gccgacgttg ggccggtgat cgcggacccg gtgcgcaccg ccgccgatgt cgccgccatg aaaccacttg atccacaagc gattcaaccg gtattggtgg cggcttcgtt gttggtcgct gagcttggcg acgtgccgct gatcggtttc gccggcgcgc cgttcacgct ggcctcctac ctcgtggagg gcggaccgag ccgccatcac gcccacgtca aggcgatgat gttggccgag ccggcgagct ggcacgcgct gatggccaag ctgaccgacc tcaccatcgc gtttctggtc ggccaaatcg acgcgggggt ggacgccatc caggtattcg actcctgggc cggggcgctc tcgccgatcg actaccgcca gtacgtgttg ccgcacagcg cccgggtgtt cgctgcgctg ggcgagcatg gcgtgccgat gacgcacttc ggggttggga ccgccgaatt gctgggcgcg atgtcggagg ccgtgacagc cggggagcgg ccaggccgag gggccgtcgt cggcgtggac tggcggaccc cgctgaccga cgccgccgcc cgggtggtgc ccggcacggc gttacagggc aacctcgatc cggcggtggt cctggcgggc tggccggcgg tggaacgtgc cgcacgtgcc gttgtcgacg acggacgccg cgccgtcgac gccggtgcgg ccggccatat cttcaacctc ggccacgggg tgctgccaga aagcgatccc gcggtgctgg ccgacctggt ctcgctcgta cactcgctaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a