SSGCID: Target Page

MysmA.01152.a

Uroporphyrinogen decarboxylase

CENTER ID:	MysmA.01152.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01152.a.A1.GE28880	Full length( MysmA.01152.a )		1	353

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01152.a.A1.PS00633	Full length( MysmA.01152.a )		1	353

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2748
RefSeq:	YP_887111.1
UniProt:	A0QW23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTRRELPES PYLAAASGRS PHRVPVWFMR QAGRSLPEYR ELRAQHRMLQ ACFDAELVCE ITMQPVRRHK VDAAILFSDI VVPLKAAGIG LDIVPDVGPV IDNPIRTLGD VQAMPALESP QVAPVAEAVR LLTAELGDVP LIGFAGAPFT LASYLVEGGP SRHHERTKAM MLGESSTWHA LMTALTDLTI AFLQAQVDAG VDALQVFDSW AGTLSLTDYR EYVLPHSSRV FATMAAAGVP MTHFGVGTAE LLGAMSEALA PGAARVVGVD WRTSLADAAA RVLPGAALQG NLDPVVLLAG WPVVEKAVRR VVEDGRAAVA AGAAGHIFNL GHGVLPATDP GIITDAVELV HSL

NT Sequence

ATGAATACCC GCCGTGAGCT GCCCGAGTCG CCCTATTTGG CCGCCGCAAG CGGCCGCAGC CCACACCGCG TGCCGGTGTG GTTCATGCGG CAGGCCGGCC GGTCGCTGCC CGAGTACCGG GAGCTGCGCG CCCAGCACAG GATGTTGCAG GCCTGCTTCG ACGCCGAGCT GGTGTGCGAG ATCACCATGC AGCCGGTCCG CAGGCACAAG GTGGACGCGG CCATCCTGTT CTCCGACATC GTCGTGCCGC TCAAGGCCGC GGGTATCGGG CTCGACATCG TGCCCGACGT CGGGCCGGTC ATCGACAACC CCATCCGCAC GCTCGGCGAC GTGCAGGCCA TGCCGGCGCT GGAGTCTCCC CAGGTGGCCC CGGTCGCCGA GGCCGTGAGG CTGCTGACCG CCGAACTGGG CGACGTCCCG CTGATCGGCT TCGCAGGCGC CCCGTTCACG TTGGCCTCCT ACCTGGTGGA GGGCGGTCCC AGCCGCCACC ACGAGCGCAC CAAGGCGATG ATGCTGGGGG AGAGCTCGAC CTGGCACGCC CTGATGACGG CGCTGACGGA TCTGACCATC GCGTTCCTGC AGGCGCAGGT GGACGCGGGC GTGGACGCGT TGCAGGTGTT CGACTCGTGG GCCGGGACGC TGTCGCTGAC CGACTACCGC GAGTACGTGC TCCCGCACAG CAGCCGCGTG TTCGCGACCA TGGCGGCCGC CGGTGTCCCG ATGACGCACT TCGGCGTCGG CACGGCCGAA CTGCTCGGCG CCATGTCGGA GGCCCTGGCC CCGGGTGCGG CCCGGGTCGT CGGCGTGGAC TGGCGGACCT CACTGGCCGA TGCCGCGGCG CGGGTGCTTC CCGGCGCCGC GCTGCAGGGC AACCTCGACC CGGTGGTGCT GCTGGCGGGC TGGCCTGTGG TCGAGAAAGC GGTGCGTCGC GTCGTCGAGG ACGGCAGGGC CGCGGTCGCG GCGGGCGCCG CCGGACACAT CTTCAATCTG GGCCACGGTG TGCTGCCCGC GACCGATCCG GGCATCATCA CCGACGCGGT GGAGTTGGTG CACTCGCTG

Details for MysmA.01152.a.A1.GE28880

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMNTRREL PESPYLAAAS GRSPHRVPVW FMRQAGRSLP EYRELRAQHR MLQACFDAEL VCEITMQPVR RHKVDAAILF SDIXVPLKAA GXGLDIVPDV GPXIDNPIRT LGDVQAMPAL ESXQVAPVAE AVRLLTAELG DVPLIGFAGA PFTLASYLVE GGPSRHHERT KAMMLGESST WHALMTALTD LTIAFLQAQV DAGVDALQVF DSWAGTLSLT DYREYVLPHS SRVFATMAAA GVPMTHFGVG TAELLGAMSE ALAPGAARVV GVDWRTSLAD AAARVLPGAA LQGNLDPVVL LAGWPVVEKA VRRVVEDGRA AVAAGAAGHI FNLGHGVLPA TDPGIITDAV ELVHSL

Validated NT Sequence

tacagcgagt gcaccaactc caccgcgtcg gtgatgatgc ccggatcggt cgcgggcagc acaccgtggc ccagattgaa gatgtgtccg gcggcgcccg ccgcgaccgc ggccctgccg tcctcgacga cgcgacgcac cgctttctcg accacaggcc agcccgccag cagcaccacc gggtcgaggt tgccctgcag cgcggcgccg ggaagcaccc gcgccgcggc atcggccagt gaggtccgcc agtccacgcc gacgacccgg gccgcacccg gggccagggc ctccgacatg gcgccgagca gttcggccgt gccgacgccg aagtgcgtca tcgggacacc ggcggccgcc atggtcgcga acacgcggct gctgtgcggg agcacgtact cgcggtagtc ggtcagcgac agcgtcccgg cccacgagtc gaacacctgc aacgcgtcca cgcccgcgtc cacctgcgcc tgcaggaacg cgatggtcag atccgtcagc gccgtcatca gggcgtgcca ggtcgagctc tcccccagca tcatcgcctt ggtgcgctcg tggtggcggc tgggaccgcc ctccaccagg taggaggcca acgtgaacgg ggcgcctgcg aagccgatca gcgggacgtc gcccagttcg gcggtcagca gcctcacggc ctcggcgacc ggggccacct gnngagactc cagcgccggc atggcctgca cgtcgccgag cgtgcggatg gggttgtcga tgancggccc gacgtcgggc acgatgtcga gcccganacc cgcggccttg agcggcacga ngatgtcgga gaacaggatg gccgcgtcca ccttgtgcct gcggaccggc tgcatggtga tctcgcacac cagctcggcg tcgaagcagg cctgcaacat cctgtgctgg gcgcgcagct cccggtactc gggcagcgac cggccggcct gccgcatgaa ccacaccggc acgcggtgtg ggctgcggcc gcttgcggcg gccaaatagg gcgactcggg cagctcacgg cgggtattca tcgaaccagg accctgggtc tgagcttcca gggtacccat angat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNTRRELPE SPYLAAASGR SPHRVPVWFM RQAGRSLPEY RELRAQHRML QACFDAELVC EITMQPVRRH KVDAAILFSD IVVPLKAAGI GLDIVPDVGP VIDNPIRTLG DVQAMPALES PQVAPVAEAV RLLTAELGDV PLIGFAGAPF TLASYLVEGG PSRHHERTKA MMLGESSTWH ALMTALTDLT IAFLQAQVDA GVDALQVFDS WAGTLSLTDY REYVLPHSSR VFATMAAAGV PMTHFGVGTA ELLGAMSEAL APGAARVVGV DWRTSLADAA ARVLPGAALQ GNLDPVVLLA GWPVVEKAVR RVVEDGRAAV AAGAAGHIFN LGHGVLPATD PGIITDAVEL VHSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatacccgc cgtgagctgc ccgagtcgcc ctatttggcc gccgcaagcg gccgcagccc acaccgcgtg ccggtgtggt tcatgcggca ggccggccgg tcgctgcccg agtaccggga gctgcgcgcc cagcacagga tgttgcaggc ctgcttcgac gccgagctgg tgtgcgagat caccatgcag ccggtccgca ggcacaaggt ggacgcggcc atcctgttct ccgacatcgt cgtgccgctc aaggccgcgg gtatcgggct cgacatcgtg cccgacgtcg ggccggtcat cgacaacccc atccgcacgc tcggcgacgt gcaggccatg ccggcgctgg agtctcccca ggtggccccg gtcgccgagg ccgtgaggct gctgaccgcc gaactgggcg acgtcccgct gatcggcttc gcaggcgccc cgttcacgtt ggcctcctac ctggtggagg gcggtcccag ccgccaccac gagcgcacca aggcgatgat gctgggggag agctcgacct ggcacgccct gatgacggcg ctgacggatc tgaccatcgc gttcctgcag gcgcaggtgg acgcgggcgt ggacgcgttg caggtgttcg actcgtgggc cgggacgctg tcgctgaccg actaccgcga gtacgtgctc ccgcacagca gccgcgtgtt cgcgaccatg gcggccgccg gtgtcccgat gacgcacttc ggcgtcggca cggccgaact gctcggcgcc atgtcggagg ccctggcccc gggtgcggcc cgggtcgtcg gcgtggactg gcggacctca ctggccgatg ccgcggcgcg ggtgcttccc ggcgccgcgc tgcagggcaa cctcgacccg gtggtgctgc tggcgggctg gcctgtggtc gagaaagcgg tgcgtcgcgt cgtcgaggac ggcagggccg cggtcgcggc gggcgccgcc ggacacatct tcaatctggg ccacggtgtg ctgcccgcga ccgatccggg catcatcacc gacgcggtgg agttggtgca ctcgctgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MysmA.01152.a.A1.PS00633

PURIFICATION DATe:	6/15/2010
CONCENTRATION:	9.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	50µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATACCCGC CGTGAGCTGC CCGAGTCGCC CTATTTGGCC GCCGCAAGCG GCCGCAGCCC ACACCGCGTG CCGGTGTGGT TCATGCGGCA GGCCGGCCGG TCGCTGCCCG AGTACCGGGA GCTGCGCGCC CAGCACAGGA TGTTGCAGGC CTGCTTCGAC GCCGAGCTGG TGTGCGAGAT CACCATGCAG CCGGTCCGCA GGCACAAGGT GGACGCGGCC ATCCTGTTCT CCGACATCGT CGTGCCGCTC AAGGCCGCGG GTATCGGGCT CGACATCGTG CCCGACGTCG GGCCGGTCAT CGACAACCCC ATCCGCACGC TCGGCGACGT GCAGGCCATG CCGGCGCTGG AGTCTCCCCA GGTGGCCCCG GTCGCCGAGG CCGTGAGGCT GCTGACCGCC GAACTGGGCG ACGTCCCGCT GATCGGCTTC GCAGGCGCCC CGTTCACGTT GGCCTCCTAC CTGGTGGAGG GCGGTCCCAG CCGCCACCAC GAGCGCACCA AGGCGATGAT GCTGGGGGAG AGCTCGACCT GGCACGCCCT GATGACGGCG CTGACGGATC TGACCATCGC GTTCCTGCAG GCGCAGGTGG ACGCGGGCGT GGACGCGTTG CAGGTGTTCG ACTCGTGGGC CGGGACGCTG TCGCTGACCG ACTACCGCGA GTACGTGCTC CCGCACAGCA GCCGCGTGTT CGCGACCATG GCGGCCGCCG GTGTCCCGAT GACGCACTTC GGCGTCGGCA CGGCCGAACT GCTCGGCGCC ATGTCGGAGG CCCTGGCCCC GGGTGCGGCC CGGGTCGTCG GCGTGGACTG GCGGACCTCA CTGGCCGATG CCGCGGCGCG GGTGCTTCCC GGCGCCGCGC TGCAGGGCAA CCTCGACCCG GTGGTGCTGC TGGCGGGCTG GCCTGTGGTC GAGAAAGCGG TGCGTCGCGT CGTCGAGGAC GGCAGGGCCG CGGTCGCGGC GGGCGCCGCC GGACACATCT TCAATCTGGG CCACGGTGTG CTGCCCGCGA CCGATCCGGG CATCATCACC GACGCGGTGG AGTTGGTGCA CTCGCTGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA