MyulA.01152.a

Uroporphyrinogen decarboxylase

CENTER ID: MyulA.01152.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01152.a.A1.GE29859 Full length( MyulA.01152.a ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3852
RefSeq: YP_906974.1
UniProt: A0PT34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTRRDLPQS PYLAAVAGRK PSRVPVWFMR QAGRSLPEYR ALRRQHSMLA ACFEPEVACE VTMQPIRRYH VDAAILFSDI VVPLRAAGVD LDIVADVGPV IAAPVRTVAD VDAIKPIDSQ SIAPVLDAVE LLVAELGDTP LIGFAGAPFT LASYLVEGGP SRHHARTKAM MLAEPATWHA LMTKLTDLTI EFLLGQIRAG VDAIQVFDSW AGMLSLADYR QYALPHSARV FATLAEHGVP MTHFGVGTAE LLGAMSEAVK PGTAKVVGVD WRTALADAAA RVQPGTALQG NLDPVVLLAG WPAVERAARA VVDDGRRAVD AGAAGYVFNL GHGVLPQTDP GVLTDLVSLV HSL
NT Sequence
ATGAGTACCC GTCGCGACCT TCCGCAGTCG CCCTATCTGG CCGCGGTGGC CGGCCGCAAA CCCAGCCGAG TACCGGTGTG GTTCATGCGG CAGGCCGGCC GCTCACTGCC GGAATACCGG GCGCTGCGCC GCCAGCACAG CATGCTGGCC GCCTGCTTCG AACCCGAGGT GGCCTGCGAG GTCACCATGC AACCGATCCG CCGCTACCAC GTCGATGCCG CGATCCTGTT CTCCGACATC GTGGTACCGC TGCGCGCGGC CGGTGTGGAC TTGGACATCG TGGCCGATGT CGGACCCGTC ATCGCTGCGC CGGTGCGTAC GGTTGCCGAT GTCGACGCCA TCAAACCCAT TGACTCCCAG TCGATTGCGC CGGTGCTCGA CGCGGTTGAG CTGTTGGTCG CCGAGCTGGG TGACACTCCG CTGATCGGAT TCGCCGGAGC GCCCTTCACG TTGGCCTCCT ACCTGGTCGA GGGCGGACCC AGCCGCCACC ATGCCCGGAC CAAGGCGATG ATGTTGGCCG AGCCCGCGAC CTGGCATGCG TTGATGACCA AGTTGACCGA CCTCACCATC GAATTTCTGC TCGGTCAGAT CCGTGCCGGC GTGGATGCCA TCCAGGTGTT CGACTCGTGG GCGGGAATGT TGTCGTTGGC CGACTACCGC CAGTACGCGT TGCCGCACAG CGCACGGGTA TTCGCCACGC TAGCCGAGCA TGGTGTTCCG ATGACGCACT TCGGGGTCGG CACCGCTGAA CTGCTGGGTG CGATGTCGGA GGCTGTCAAG CCGGGGACGG CGAAGGTGGT CGGGGTGGAC TGGCGCACCG CGCTGGCCGA TGCCGCCGCC CGGGTGCAAC CCGGCACCGC GCTGCAGGGC AACCTGGATC CGGTGGTGCT GCTGGCGGGC TGGCCCGCGG TGGAACGGGC CGCACGCGCC GTCGTCGACG ATGGACGTCG GGCCGTCGAC GCGGGTGCGG CCGGGTACGT CTTCAATCTG GGGCACGGGG TACTGCCGCA GACCGATCCG GGGGTGCTGA CCGACCTGGT GTCACTGGTT CACTCGCTA
Details for MyulA.01152.a.A1.GE29859
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 91
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTRRDLPQ SPYLAAVAGR KPSRVPVWFM RQAGRSLPEY RALRRQHSML AACFEPEVAC EVTMQPIRRY HVDAAILFSD IVVPLRAAGV DLDIVADVGP VIAAPVRTVA DVDAIKPIDS QSIAPVLDAV ELLVAELGDT PLIGFAGAPF TLASYLVEGG PSRHHARTKA MMLAEPATWH ALMTKLXDLT IEFLLGQIRA GVXAIXXSTR GRECCRWXTX ASTRCRTXHG YSPR
Validated NT Sequence
tnnnnntant ttangaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagtacc cgtcgcgacc ttccgcagtc gccctatctg gccgcggtgg ccggccgcaa acccagccga gtaccggtgt ggttcatgcg gcaggccggc cgctcactgc cggaataccg ggcgctgcgc cgccagcaca gcatgctggc cgcctgcttc gaacccgagg tggcctgcga ggtcaccatg caaccgatcc gccgctacca cgtcgatgcc gcgatcctgt tctccgacat cgtggtaccg ctgcgcgcgg ccggtgtgga cttggacatc gtggccgatg tcggacccgt catcgctgcg ccggtgcgta cggttgccga tgtcgacgcc atcaaaccca ttgactccca gtcgattgcg ccggtgctcg acgcggttga gctgttggtc gccgagctgg gtgacactcc gctgatcgga ttcgccggag cgcccttcac gttggcctcc tacctggtcg agggcggacc cagccgccac catgcccgga ccaaggcgat gatgttggcc gagcccgcga cctggcatgc gttgatgacc aagttgancg acctcaccat cgaatttctg ctcggtcaga tccgtgccgg cgtgnntgcc atccnnngtt cnactcgtgg gcgggaatgt tgtcgttggn cgactancgc cagtacgcgt tgccgcacan cgcacgggta ttcgccacgc tagccganca tggngnnccn atgangcact tcgggtcggc ancnctgaan tgctggngng atgtnnnagn tgtcnagcnn gnnnaannng ncggnnngan tnnncncnnn ntncgnngcn cnnccnnnnn ancnnncnnc gnntnnnngn nnngnncnnn nnnntgctgc nnnngnnnnn nnnnnnnnnc nnnnnnnnan gnnntnnnnn nnnnncngnn ngnnnnnntn annngnnnng nnnnnncnnn ncnnnngnnn nnnannacnn nnnnnnnncn nnnannnnnn annnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTRRDLPQ SPYLAAVAGR KPSRVPVWFM RQAGRSLPEY RALRRQHSML AACFEPEVAC EVTMQPIRRY HVDAAILFSD IVVPLRAAGV DLDIVADVGP VIAAPVRTVA DVDAIKPIDS QSIAPVLDAV ELLVAELGDT PLIGFAGAPF TLASYLVEGG PSRHHARTKA MMLAEPATWH ALMTKLTDLT IEFLLGQIRA GVDAIQVFDS WAGMLSLADY RQYALPHSAR VFATLAEHGV PMTHFGVGTA ELLGAMSEAV KPGTAKVVGV DWRTALADAA ARVQPGTALQ GNLDPVVLLA GWPAVERAAR AVVDDGRRAV DAGAAGYVFN LGHGVLPQTD PGVLTDLVSL VHS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtacccgt cgcgaccttc cgcagtcgcc ctatctggcc gcggtggccg gccgcaaacc cagccgagta ccggtgtggt tcatgcggca ggccggccgc tcactgccgg aataccgggc gctgcgccgc cagcacagca tgctggccgc ctgcttcgaa cccgaggtgg cctgcgaggt caccatgcaa ccgatccgcc gctaccacgt cgatgccgcg atcctgttct ccgacatcgt ggtaccgctg cgcgcggccg gtgtggactt ggacatcgtg gccgatgtcg gacccgtcat cgctgcgccg gtgcgtacgg ttgccgatgt cgacgccatc aaacccattg actcccagtc gattgcgccg gtgctcgacg cggttgagct gttggtcgcc gagctgggtg acactccgct gatcggattc gccggagcgc ccttcacgtt ggcctcctac ctggtcgagg gcggacccag ccgccaccat gcccggacca aggcgatgat gttggccgag cccgcgacct ggcatgcgtt gatgaccaag ttgaccgacc tcaccatcga atttctgctc ggtcagatcc gtgccggcgt ggatgccatc caggtgttcg actcgtgggc gggaatgttg tcgttggccg actaccgcca gtacgcgttg ccgcacagcg cacgggtatt cgccacgcta gccgagcatg gtgttccgat gacgcacttc ggggtcggca ccgctgaact gctgggtgcg atgtcggagg ctgtcaagcc ggggacggcg aaggtggtcg gggtggactg gcgcaccgcg ctggccgatg ccgccgcccg ggtgcaaccc ggcaccgcgc tgcagggcaa cctggatccg gtggtgctgc tggcgggctg gcccgcggtg gaacgggccg cacgcgccgt cgtcgacgat ggacgtcggg ccgtcgacgc gggtgcggcc gggtacgtct tcaatctggg gcacggggta ctgccgcaga ccgatccggg ggtgctgacc gacctggtgt cactggttca ctcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa