MyabA.01152.a

Uroporphyrinogen decarboxylase

CENTER ID: MyabA.01152.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01152.a.A1.GE34912 Full length( MyabA.01152.a ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01152.a.A1.PW37382 Full length( MyabA.01152.a ) 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3036
RefSeq: YP_001703718.1
UniProt: B1MCU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGSAPGRHA PAGAPCEAVM AAWAVMTGLN EDSATAAQTT SKTRRDLPLS PYLVAAAGGV PSHPPVWFMR QAGRSLPEYR AVRADHAMLD ACFNPELVCE ITLQPIRRHR VDAAILFSDI VVPLKAAGID LDIVPDVGPV IANPVRTTAD VAALPRLEPG QVEAVSAAVR LLVGELGATP LIGFAGAPFT LASYLVEGGP SRHHQRTKAL LLGDPQTWHA LMTALTDITI AFLRAQLDAG VDALQLFDSW AGALSLADYR AAVLPHSTRI FQEFAGVGVP MTHFGVGTAE LLGAMGEAGA TVVGVDWRTP LNVAASRVGS GKALQGNLDP AVLLAGWPVV ESEVRRIVAE GRQAVADGAA GHIFNLGHGV LPDTDPAVLT DAAALVHSL
NT Sequence
ATGGACGGTA GCGCGCCGGG CCGACATGCT CCCGCAGGCG CGCCGTGCGA AGCCGTCATG GCAGCATGGG CTGTGATGAC CGGATTGAAC GAAGATAGCG CCACGGCGGC GCAAACCACT TCGAAAACGC GGCGTGACCT GCCTCTTTCG CCCTATTTGG TGGCTGCCGC GGGCGGGGTG CCCAGCCACC CGCCGGTGTG GTTCATGCGG CAAGCCGGAC GTTCGCTGCC GGAGTACCGG GCGGTGCGAG CCGATCACGC CATGCTGGAC GCTTGTTTTA ACCCCGAACT GGTTTGCGAG ATCACACTGC AGCCGATCCG TCGGCACCGT GTGGATGCCG CCATTCTCTT CTCGGACATC GTGGTGCCAC TCAAGGCCGC CGGAATCGAC CTGGACATCG TTCCGGATGT CGGGCCCGTG ATCGCCAACC CGGTGCGCAC CACCGCGGAT GTGGCGGCGC TGCCGCGGCT GGAGCCCGGA CAGGTCGAGG CCGTCAGCGC CGCGGTGCGG CTACTGGTCG GCGAGCTCGG CGCCACCCCG CTGATCGGCT TCGCCGGTGC GCCCTTCACA TTGGCTTCCT ACCTCGTGGA GGGCGGCCCG AGCCGTCACC ATCAGCGCAC CAAGGCCCTG CTGCTGGGCG ATCCGCAGAC CTGGCATGCG TTGATGACGG CGTTGACCGA CATCACCATC GCGTTTCTGC GGGCGCAGCT CGACGCGGGA GTGGACGCGC TGCAGTTGTT CGACTCGTGG GCGGGCGCAC TGTCCCTGGC CGATTACCGC GCTGCGGTGT TGCCGCACAG CACAAGGATT TTCCAGGAGT TCGCGGGAGT CGGTGTGCCG ATGACGCACT TCGGGGTGGG CACCGCCGAG CTGCTGGGGG CGATGGGGGA GGCCGGCGCC ACGGTGGTGG GGGTGGACTG GCGCACCCCG CTGAATGTCG CGGCGAGCCG AGTTGGTTCC GGAAAAGCCT TGCAGGGCAA CCTCGATCCT GCCGTGCTGT TGGCGGGATG GCCGGTAGTG GAGAGTGAAG TTCGCCGTAT CGTCGCCGAG GGACGCCAGG CGGTGGCTGA CGGCGCCGCC GGGCATATCT TCAATCTGGG GCATGGCGTG CTGCCGGATA CCGATCCGGC GGTACTGACC GATGCGGCGG CGCTGGTGCA TTCGTTG
Details for MyabA.01152.a.A1.GE34912
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDGSAPGRH APAGAPCEAV MAAWAVMTGL NEDSATAAQT TSKTRRDLPL SPYLVAAAGG VPSHPPVWFM RQAGRSLPEY RAVRADHAML DACFNPELVC EITLQPIRRH RVDAAILFSD IVVPLKAAGI DLDIVPDVGP VIANPVRTTA DVAALPRLEP GQVEAVSAAV RLLVGELGAT PLIGFAGAPF TLASYLVEGG PSRHHQRTKA LLLGDPQTWH ALMTALTDIT IAFLRAQLDA GVDALQLFDS WAGALSLADY RAAVLPHSTR IFQEFAGVGV PMTHFGVGTA ELLGAMGEAG ATVVGVDWRT PLNVAASRVG SGKALQGNLD PAVLLAGWPV VESEVRRIVA EGRQAVADGA AGHIFNLGHG VLPDTDPAVL TDAAALVHSL
Validated NT Sequence
ttattacaac gaatgcacca gcgccgccgc atcggtcagt accgccggat cggtatccgg cagcacgcca tgccccagat tgaagatatg cccggcggcg ccgtcagcca ccgcctggcg tccctcggcg acgatacggc gaacttcact ctccactacc ggccatcccg ccaacagcac ggcaggatcg aggttgccct gcaaggcttt tccggaacca actcggctcg ccgcgacatt cagcggggtg cgccagtcca cccccaccac cgtggcgccg gcctccccca tcgcccccag cagctcggcg gtgcccaccc cgaagtgcgt catcggcaca ccgactcccg cgaactcctg gaaaatcctt gtgctgtgcg gcaacaccgc agcgcggtaa tcggccaggg acagtgcgcc cgcccacgag tcgaacaact gcagcgcgtc cactcccgcg tcgagctgcg cccgcagaaa cgcgatggtg atgtcggtca acgccgtcat caacgcatgc caggtctgcg gatcgcccag cagcagggcc ttggtgcgct gatggtgacg gctcgggccg ccctccacga ggtaggaagc caatgtgaag ggcgcaccgg cgaagccgat cagcggggtg gcgccgagct cgccgaccag tagccgcacc gcggcgctga cggcctcgac ctgtccgggc tccagccgcg gcagcgccgc cacatccgcg gtggtgcgca ccgggttggc gatcacgggc ccgacatccg gaacgatgtc caggtcgatt ccggcggcct tgagtggcac cacgatgtcc gagaagagaa tggcggcatc cacacggtgc cgacggatcg gctgcagtgt gatctcgcaa accagttcgg ggttaaaaca agcgtccagc atggcgtgat cggctcgcac cgcccggtac tccggcagcg aacgtccggc ttgccgcatg aaccacaccg gcgggtggct gggcaccccg cccgcggcag ccaccaaata gggcgaaaga ggcaggtcac gccgcgtttt cgaagtggtt tgcgccgccg tggcgctatc ttcgttcaat ccggtcatca cagcccatgc tgccatgacg gcttcgcacg gcgcgcctgc gggagcatgt cggcccggcg cgctaccgtc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDGSAPGRH APAGAPCEAV MAAWAVMTGL NEDSATAAQT TSKTRRDLPL SPYLVAAAGG VPSHPPVWFM RQAGRSLPEY RAVRADHAML DACFNPELVC EITLQPIRRH RVDAAILFSD IVVPLKAAGI DLDIVPDVGP VIANPVRTTA DVAALPRLEP GQVEAVSAAV RLLVGELGAT PLIGFAGAPF TLASYLVEGG PSRHHQRTKA LLLGDPQTWH ALMTALTDIT IAFLRAQLDA GVDALQLFDS WAGALSLADY RAAVLPHSTR IFQEFAGVGV PMTHFGVGTA ELLGAMGEAG ATVVGVDWRT PLNVAASRVG SGKALQGNLD PAVLLAGWPV VESEVRRIVA EGRQAVADGA AGHIFNLGHG VLPDTDPAVL TDAAALVHSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacggtagc gcgccgggcc gacatgctcc cgcaggcgcg ccgtgcgaag ccgtcatggc agcatgggct gtgatgaccg gattgaacga agatagcgcc acggcggcgc aaaccacttc gaaaacgcgg cgtgacctgc ctctttcgcc ctatttggtg gctgccgcgg gcggggtgcc cagccacccg ccggtgtggt tcatgcggca agccggacgt tcgctgccgg agtaccgggc ggtgcgagcc gatcacgcca tgctggacgc ttgttttaac cccgaactgg tttgcgagat cacactgcag ccgatccgtc ggcaccgtgt ggatgccgcc attctcttct cggacatcgt ggtgccactc aaggccgccg gaatcgacct ggacatcgtt ccggatgtcg ggcccgtgat cgccaacccg gtgcgcacca ccgcggatgt ggcggcgctg ccgcggctgg agcccggaca ggtcgaggcc gtcagcgccg cggtgcggct actggtcggc gagctcggcg ccaccccgct gatcggcttc gccggtgcgc ccttcacatt ggcttcctac ctcgtggagg gcggcccgag ccgtcaccat cagcgcacca aggccctgct gctgggcgat ccgcagacct ggcatgcgtt gatgacggcg ttgaccgaca tcaccatcgc gtttctgcgg gcgcagctcg acgcgggagt ggacgcgctg cagttgttcg actcgtgggc gggcgcactg tccctggccg attaccgcgc tgcggtgttg ccgcacagca caaggatttt ccaggagttc gcgggagtcg gtgtgccgat gacgcacttc ggggtgggca ccgccgagct gctgggggcg atgggggagg ccggcgccac ggtggtgggg gtggactggc gcaccccgct gaatgtcgcg gcgagccgag ttggttccgg aaaagccttg cagggcaacc tcgatcctgc cgtgctgttg gcgggatggc cggtagtgga gagtgaagtt cgccgtatcg tcgccgaggg acgccaggcg gtggctgacg gcgccgccgg gcatatcttc aatctggggc atggcgtgct gccggatacc gatccggcgg tactgaccga tgcggcggcg ctggtgcatt cgttgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyabA.01152.a.A1.PW37382
PURIFICATION DATe: 10/28/2013
CONCENTRATION: 11.3mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDGSAPGRH APAGAPCEAV MAAWAVMTGL NEDSATAAQT TSKTRRDLPL SPYLVAAAGG VPSHPPVWFM RQAGRSLPEY RAVRADHAML DACFNPELVC EITLQPIRRH RVDAAILFSD IVVPLKAAGI DLDIVPDVGP VIANPVRTTA DVAALPRLEP GQVEAVSAAV RLLVGELGAT PLIGFAGAPF TLASYLVEGG PSRHHQRTKA LLLGDPQTWH ALMTALTDIT IAFLRAQLDA GVDALQLFDS WAGALSLADY RAAVLPHSTR IFQEFAGVGV PMTHFGVGTA ELLGAMGEAG ATVVGVDWRT PLNVAASRVG SGKALQGNLD PAVLLAGWPV VESEVRRIVA EGRQAVADGA AGHIFNLGHG VLPDTDPAVL TDAAALVHSL
Validated NT Sequence
ttattacaac gaatgcacca gcgccgccgc atcggtcagt accgccggat cggtatccgg cagcacgcca tgccccagat tgaagatatg cccggcggcg ccgtcagcca ccgcctggcg tccctcggcg acgatacggc gaacttcact ctccactacc ggccatcccg ccaacagcac ggcaggatcg aggttgccct gcaaggcttt tccggaacca actcggctcg ccgcgacatt cagcggggtg cgccagtcca cccccaccac cgtggcgccg gcctccccca tcgcccccag cagctcggcg gtgcccaccc cgaagtgcgt catcggcaca ccgactcccg cgaactcctg gaaaatcctt gtgctgtgcg gcaacaccgc agcgcggtaa tcggccaggg acagtgcgcc cgcccacgag tcgaacaact gcagcgcgtc cactcccgcg tcgagctgcg cccgcagaaa cgcgatggtg atgtcggtca acgccgtcat caacgcatgc caggtctgcg gatcgcccag cagcagggcc ttggtgcgct gatggtgacg gctcgggccg ccctccacga ggtaggaagc caatgtgaag ggcgcaccgg cgaagccgat cagcggggtg gcgccgagct cgccgaccag tagccgcacc gcggcgctga cggcctcgac ctgtccgggc tccagccgcg gcagcgccgc cacatccgcg gtggtgcgca ccgggttggc gatcacgggc ccgacatccg gaacgatgtc caggtcgatt ccggcggcct tgagtggcac cacgatgtcc gagaagagaa tggcggcatc cacacggtgc cgacggatcg gctgcagtgt gatctcgcaa accagttcgg ggttaaaaca agcgtccagc atggcgtgat cggctcgcac cgcccggtac tccggcagcg aacgtccggc ttgccgcatg aaccacaccg gcgggtggct gggcaccccg cccgcggcag ccaccaaata gggcgaaaga ggcaggtcac gccgcgtttt cgaagtggtt tgcgccgccg tggcgctatc ttcgttcaat ccggtcatca cagcccatgc tgccatgacg gcttcgcacg gcgcgcctgc gggagcatgt cggcccggcg cgctaccgtc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDGSAPGRH APAGAPCEAV MAAWAVMTGL NEDSATAAQT TSKTRRDLPL SPYLVAAAGG VPSHPPVWFM RQAGRSLPEY RAVRADHAML DACFNPELVC EITLQPIRRH RVDAAILFSD IVVPLKAAGI DLDIVPDVGP VIANPVRTTA DVAALPRLEP GQVEAVSAAV RLLVGELGAT PLIGFAGAPF TLASYLVEGG PSRHHQRTKA LLLGDPQTWH ALMTALTDIT IAFLRAQLDA GVDALQLFDS WAGALSLADY RAAVLPHSTR IFQEFAGVGV PMTHFGVGTA ELLGAMGEAG ATVVGVDWRT PLNVAASRVG SGKALQGNLD PAVLLAGWPV VESEVRRIVA EGRQAVADGA AGHIFNLGHG VLPDTDPAVL TDAAALVHSL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGGTAGC GCGCCGGGCC GACATGCTCC CGCAGGCGCG CCGTGCGAAG CCGTCATGGC AGCATGGGCT GTGATGACCG GATTGAACGA AGATAGCGCC ACGGCGGCGC AAACCACTTC GAAAACGCGG CGTGACCTGC CTCTTTCGCC CTATTTGGTG GCTGCCGCGG GCGGGGTGCC CAGCCACCCG CCGGTGTGGT TCATGCGGCA AGCCGGACGT TCGCTGCCGG AGTACCGGGC GGTGCGAGCC GATCACGCCA TGCTGGACGC TTGTTTTAAC CCCGAACTGG TTTGCGAGAT CACACTGCAG CCGATCCGTC GGCACCGTGT GGATGCCGCC ATTCTCTTCT CGGACATCGT GGTGCCACTC AAGGCCGCCG GAATCGACCT GGACATCGTT CCGGATGTCG GGCCCGTGAT CGCCAACCCG GTGCGCACCA CCGCGGATGT GGCGGCGCTG CCGCGGCTGG AGCCCGGACA GGTCGAGGCC GTCAGCGCCG CGGTGCGGCT ACTGGTCGGC GAGCTCGGCG CCACCCCGCT GATCGGCTTC GCCGGTGCGC CCTTCACATT GGCTTCCTAC CTCGTGGAGG GCGGCCCGAG CCGTCACCAT CAGCGCACCA AGGCCCTGCT GCTGGGCGAT CCGCAGACCT GGCATGCGTT GATGACGGCG TTGACCGACA TCACCATCGC GTTTCTGCGG GCGCAGCTCG ACGCGGGAGT GGACGCGCTG CAGTTGTTCG ACTCGTGGGC GGGCGCACTG TCCCTGGCCG ATTACCGCGC TGCGGTGTTG CCGCACAGCA CAAGGATTTT CCAGGAGTTC GCGGGAGTCG GTGTGCCGAT GACGCACTTC GGGGTGGGCA CCGCCGAGCT GCTGGGGGCG ATGGGGGAGG CCGGCGCCAC GGTGGTGGGG GTGGACTGGC GCACCCCGCT GAATGTCGCG GCGAGCCGAG TTGGTTCCGG AAAAGCCTTG CAGGGCAACC TCGATCCTGC CGTGCTGTTG GCGGGATGGC CGGTAGTGGA GAGTGAAGTT CGCCGTATCG TCGCCGAGGG ACGCCAGGCG GTGGCTGACG GCGCCGCCGG GCATATCTTC AATCTGGGGC ATGGCGTGCT GCCGGATACC GATCCGGCGG TACTGACCGA TGCGGCGGCG CTGGTGCATT CGTTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA