SSGCID: Target Page

MypaA.01152.a

Uroporphyrinogen decarboxylase

CENTER ID:	MypaA.01152.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01152.a.A1.GE29554	Full length( MypaA.01152.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.2965
RefSeq:	NP_961733.1
UniProt:	Q73W61

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSSARTRRD LPESPYLAAV NGRKPHRVPV WFMRQAGRSL PEYRALRAQH DMLSACFDAE LVCEITLQPV RRHDVDAAIL FSDIVVPLLG AGIDLDIVAG VGPVIASPVR TAADVAAMKP LERQRVQPIA DAVRLLVSAL VEVPLIGFAG APFTLASYLI EGGPSRHHAR TKAMMLADPD SWHALMEKLT DITVGFLRVQ LDAGVDAVQV FDSWAGALSL ANYRSYVQPH SARVFAALAD YGVPMTHFGV GTAELLGAMS AALKPAPATV VGVDWRTALA DAATRVMPGT ALQGNLDPVV LLAGWPVVET AARAVVDDGR RAVDAGAAGH VFNLGHGVLP ETDPGVLTEL VSLVHSL

NT Sequence

ATGAACAGCA GCGCGCGTAC CCGCCGCGAC CTTCCCGAGT CGCCGTATCT GGCCGCGGTG AACGGCCGCA AACCCCACCG GGTTCCGGTG TGGTTCATGC GGCAGGCCGG CCGGTCGCTG CCCGAATACC GGGCGCTGCG GGCTCAGCAC GACATGCTGT CGGCCTGTTT CGACGCCGAG CTGGTCTGCG AGATCACGCT GCAGCCGGTG CGCCGGCACG ACGTCGACGC GGCGATCCTG TTCTCCGACA TCGTGGTCCC GCTGCTGGGC GCCGGCATCG ACCTGGACAT CGTGGCGGGC GTCGGGCCGG TGATCGCGTC GCCGGTGCGC ACCGCCGCGG ACGTGGCCGC GATGAAACCG CTTGAGCGGC AACGGGTTCA GCCGATCGCC GATGCGGTGC GGCTGCTGGT GTCCGCGCTC GTTGAGGTTC CGCTGATCGG CTTCGCGGGT GCGCCGTTCA CGCTGGCGTC CTATCTGATC GAGGGCGGGC CCAGCCGCCA CCACGCCCGC ACCAAGGCGA TGATGCTGGC CGACCCGGAC AGCTGGCACG CCCTGATGGA GAAGCTGACC GACATCACCG TGGGCTTCCT GCGGGTGCAA CTGGACGCCG GGGTGGACGC CGTCCAGGTG TTCGACTCGT GGGCCGGGGC GCTGTCGCTG GCCAACTACC GCAGCTACGT GCAGCCGCAC AGCGCCCGGG TGTTCGCGGC GCTGGCCGAT TACGGCGTGC CGATGACACA CTTCGGGGTG GGCACCGCCG AACTGTTGGG GGCGATGTCG GCCGCCCTCA AACCGGCGCC CGCGACGGTG GTGGGCGTGG ACTGGCGCAC CGCGCTCGCC GACGCGGCGA CCCGGGTGAT GCCCGGCACC GCGCTGCAGG GCAACCTCGA TCCGGTGGTG CTGCTGGCCG GGTGGCCGGT GGTGGAGACG GCGGCGCGGG CGGTGGTGGA CGACGGGCGG CGCGCCGTCG ACGCCGGCGC GGCCGGGCAC GTGTTCAACC TCGGCCACGG GGTGCTGCCC GAAACCGATC CCGGTGTGCT GACCGAGCTG GTGTCACTGG TCCACTCGCT A

Details for MypaA.01152.a.A1.GE29554

HARVESTED ON:	6/15/2010
SEQUENCED ON:	7/8/2010
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	10

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNSSARTRR DLPXXRIWPR

Validated NT Sequence

ttntttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaacagc agcgcgcgta cccgccgcga ccttcccann ngccgtatct ggccgcggtg aacggccgca aaccccaccg ggttccggtg tggttcatgc ggcaggccgg ccggtcgctg cccgaatacc gggcgctgcg ggctcagcac gacatgctgt cggcctgttt cgacgccgag ctggtctgcg agatcacgct gcagccggtg cgccggcacg acgtcgacgc ggcgatcctg ttctccgaca tcgtggtccc gctgctgggc gccggcatcg acctggacat cgtggcgggc gtcgggccgg tgatcgcgtc gccggtgcgc accgccgcgg acgtggccgc gatgaaaccg cttgagcggc aacgggttca gccgatcgcc gatgcggtgc ggctgctggt gtccgcgctc gttgaggttc cgctgatcgg cttcgcgggt gcgccgttca cgctggcgtc ctatctgatc gagggcgggc ccagccgcca ccacgcccgc accaaggcga tgatgctggc cgacccggac agctggcacg ccctgatgga gaagctgacc gacatcaccg tgggcttcct gcgggtgcaa ctggacgccg gggtggacgc cgtccaggtg ttcgactcgt gggccggggc gctgtcgctg gccaactacc gcagctacgt gcagccgcac agcgcccggg tgttcgcggc gctggncgat tacggcgtgc cgatgacaca cttcggggtg ggcancgccg aactgttggg ggcgatgtcg gccgccctca aaccggcgcc cgcgacggtg gtgggcgtgg nctggcgcac cgcgctcgcc gacgcggcga cccnggtgat gcccggnacc gcgnnnncag ggnannctcg atccngtggn gctgctgnnn ngnnnnnnnn ngnnnnnnnn nnnncgncnn gntggnnnan nnnnnnncnn nnnnncnncg nncgnnngnn nnnnnnnnnn nggggnnnnt ncnnnncnnn tnnnnnnnnn ngannnnnnn nnnnnnnnnt nnnnnnnncn nnnnnnnnng nnnnnnnnnn nnnannnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNSSARTRR DLPESPYLAA VNGRKPHRVP VWFMRQAGRS LPEYRALRAQ HDMLSACFDA ELVCEITLQP VRRHDVDAAI LFSDIVVPLL GAGIDLDIVA GVGPVIASPV RTAADVAAMK PLERQRVQPI ADAVRLLVSA LVEVPLIGFA GAPFTLASYL IEGGPSRHHA RTKAMMLADP DSWHALMEKL TDITVGFLRV QLDAGVDAVQ VFDSWAGALS LANYRSYVQP HSARVFAALA DYGVPMTHFG VGTAELLGAM SAALKPAPAT VVGVDWRTAL ADAATRVMPG TALQGNLDPV VLLAGWPVVE TAARAVVDDG RRAVDAGAAG HVFNLGHGVL PETDPGVLTE LVSLVHSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacagcagc gcgcgtaccc gccgcgacct tcccgagtcg ccgtatctgg ccgcggtgaa cggccgcaaa ccccaccggg ttccggtgtg gttcatgcgg caggccggcc ggtcgctgcc cgaataccgg gcgctgcggg ctcagcacga catgctgtcg gcctgtttcg acgccgagct ggtctgcgag atcacgctgc agccggtgcg ccggcacgac gtcgacgcgg cgatcctgtt ctccgacatc gtggtcccgc tgctgggcgc cggcatcgac ctggacatcg tggcgggcgt cgggccggtg atcgcgtcgc cggtgcgcac cgccgcggac gtggccgcga tgaaaccgct tgagcggcaa cgggttcagc cgatcgccga tgcggtgcgg ctgctggtgt ccgcgctcgt tgaggttccg ctgatcggct tcgcgggtgc gccgttcacg ctggcgtcct atctgatcga gggcgggccc agccgccacc acgcccgcac caaggcgatg atgctggccg acccggacag ctggcacgcc ctgatggaga agctgaccga catcaccgtg ggcttcctgc gggtgcaact ggacgccggg gtggacgccg tccaggtgtt cgactcgtgg gccggggcgc tgtcgctggc caactaccgc agctacgtgc agccgcacag cgcccgggtg ttcgcggcgc tggccgatta cggcgtgccg atgacacact tcggggtggg caccgccgaa ctgttggggg cgatgtcggc cgccctcaaa ccggcgcccg cgacggtggt gggcgtggac tggcgcaccg cgctcgccga cgcggcgacc cgggtgatgc ccggcaccgc gctgcagggc aacctcgatc cggtggtgct gctggccggg tggccggtgg tggagacggc ggcgcgggcg gtggtggacg acgggcggcg cgccgtcgac gccggcgcgg ccgggcacgt gttcaacctc ggccacgggg tgctgcccga aaccgatccc ggtgtgctga ccgagctggt gtcactggtc cactcgctaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a