SSGCID: Target Page

MyleA.01152.a

Uroporphyrinogen decarboxylase

CENTER ID:	MyleA.01152.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01152.a.A1.GE29255	Full length( MyleA.01152.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1879
RefSeq:	YP_002503399.1
UniProt:	B8ZQX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTRRELLES PYLAAVSGRK PCRVPVWFMR QAGRSLPEYR ALRERYSMLA ACFEPEVACE ITLQPLRRYD VDAAILFSDI VVPLCAAGID LDIVPDVGPV IGDPVRTATD IHAMKPLEPQ AIQPIFQAIS LLVAALGDVP LIGFAGAPFT LASYLVEGGP SRNHPRTKAM MLAEPASWHT LMDKLTDLTL GFLLGQIDAG VDAIQVFDSW AGTLSLSDYR QYVLPHSARI FATVAEHGVP MTHFGVGTAD LLGAMSAAVR SGEKPGHQAV VGVDWRTSLT DAAARVEPCT ALQGNLDPVV LLAGWPAVER VARTVVDDGR RAVVAGAAGH VFNLGHGVLP ETDPGVLSEL VSFIHSL

NT Sequence

ATGAGTACCC GTCGCGAGCT TCTCGAATCG CCGTATCTGG CCGCGGTCAG TGGACGCAAA CCCTGCCGGG TTCCGGTGTG GTTTATGCGG CAAGCCGGCC GCTCGTTGCC TGAATACCGG GCGCTGCGCG AGCGATACAG CATGTTGGCG GCGTGCTTCG AGCCCGAAGT GGCCTGTGAA ATCACTCTGC AGCCGTTACG TCGTTACGAC GTTGATGCCG CGATCTTGTT CTCAGACATC GTGGTGCCGC TGTGCGCGGC AGGAATCGAT CTGGATATTG TGCCAGATGT CGGTCCAGTG ATCGGGGACC CGGTCCGGAC TGCCACCGAT ATCCACGCTA TGAAACCCCT TGAACCACAA GCGATTCAGC CTATTTTTCA GGCTATTTCG TTGCTGGTCG CAGCACTGGG TGATGTTCCG CTGATCGGTT TCGCCGGTGC GCCGTTTACG CTGGCGTCCT ACCTGGTCGA AGGCGGTCCT AGTCGTAATC ATCCCCGCAC TAAGGCCATG ATGCTGGCCG AACCTGCGAG CTGGCATACG CTGATGGATA AGCTGACCGA TCTGACTCTT GGATTTTTGC TTGGTCAAAT CGACGCCGGA GTTGATGCCA TCCAGGTATT CGACTCGTGG GCGGGAACGC TGTCCCTGTC CGATTACCGC CAGTACGTGT TGCCACATAG CGCCCGGATT TTTGCCACGG TGGCCGAACA CGGGGTGCCG ATGACGCACT TCGGGGTTGG CACCGCCGAC CTGCTGGGTG CGATGTCAGC GGCTGTGCGG TCAGGCGAAA AGCCTGGGCA CCAGGCCGTC GTCGGCGTGG ATTGGCGAAC CTCGTTGACC GATGCTGCTG CCCGAGTGGA ACCCTGTACC GCGCTGCAGG GCAACCTCGA CCCGGTGGTG CTGTTAGCGG GCTGGCCGGC TGTCGAACGT GTCGCACGGA CTGTCGTCGA CGACGGGCGC CGTGCCGTCG TGGCCGGCGC GGCTGGCCAC GTTTTCAATC TCGGCCATGG AGTGTTGCCG GAGACCGATC CCGGCGTGCT GAGCGAATTA GTATCGTTCA TCCACTCGCT A

Details for MyleA.01152.a.A1.GE29255

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

atagcgagtg gatgaacgat actaattcgc tcagcacgcc gggatcggtc tccggcaaca ctccatggcc gagattgaaa acgtggccag ccgcgccggc cacgacggca cggcgcccgt cgtcgacgac agtccgtgcg acacgttcga cagccggcca gcccgctaac agcaccaccg ggtcgaggtt gccctgcagc gcggtacagg gttccactcg ggcagcagca tcggtcaacg aggttcgcca atccacgccg acgacggcct ggtgcccagg cttttcgcct gaccgcacag ccgctgacat cgcacccagc aggtcggcgg tgccaacccc gaagtgcgtc atcggcaccc cgtgttcggc caccgtggca aaaatccggg cgctatgtgg caacacgtac tggcggtaat cggacaggga cagcgttccc gcccacgagt cgaatacctg gatggcatca actccggcgt cgatttgacc aagcaaaaat ccaagagtca gatcggtcag cttatccatc agcgtatgcc agctcgcagg ttcggccagc atcatggcct tagtgcgggg atgattacga ctaggaccgc cttcgaccag gtaggacgcc agcgtaaacg gcgcaccggc gaaaccgatc agcggaacat cacccagtgc tgcgaccagc aacgaaatag cctgaaaaat aggctgaatc gcttgtggtt caaggggttt catagcgtgg atatcggtgg cagtccggac cgggtccccg atcactggac cgacatctgg cacaatatcc agatcgattc ctgccgcgca cagcggcacc acgatgtctg agaacaagat cgcggcatca acgtcgtaac gacgtaacgg ctgcagagtg atttcacagg ccacttcggg ctcgaagcac gccgccaaca tgctgtatcg ctcgcgcagc gcccggtatt caggcaacga gcggccggct tgccgcataa accacaccgg aacccggcag ggtttgcgtc cactgaccgc ggccagatac ggcgattcga gaagctcgcg acgggtactc atcgaaccag gaccctgggt ctgagcttcc ag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTRRELLE SPYLAAVSGR KPCRVPVWFM RQAGRSLPEY RALRERYSML AACFEPEVAC EITLQPLRRY DVDAAILFSD IVVPLCAAGI DLDIVPDVGP VIGDPVRTAT DIHAMKPLEP QAIQPIFQAI SLLVAALGDV PLIGFAGAPF TLASYLVEGG PSRNHPRTKA MMLAEPASWH TLMDKLTDLT LGFLLGQIDA GVDAIQVFDS WAGTLSLSDY RQYVLPHSAR IFATVAEHGV PMTHFGVGTA DLLGAMSAAV RSGEKPGHQA VVGVDWRTSL TDAAARVEPC TALQGNLDPV VLLAGWPAVE RVARTVVDDG RRAVVAGAAG HVFNLGHGVL PETDPGVLSE LVSFIHSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtacccgt cgcgagcttc tcgaatcgcc gtatctggcc gcggtcagtg gacgcaaacc ctgccgggtt ccggtgtggt ttatgcggca agccggccgc tcgttgcctg aataccgggc gctgcgcgag cgatacagca tgttggcggc gtgcttcgag cccgaagtgg cctgtgaaat cactctgcag ccgttacgtc gttacgacgt tgatgccgcg atcttgttct cagacatcgt ggtgccgctg tgcgcggcag gaatcgatct ggatattgtg ccagatgtcg gtccagtgat cggggacccg gtccggactg ccaccgatat ccacgctatg aaaccccttg aaccacaagc gattcagcct atttttcagg ctatttcgtt gctggtcgca gcactgggtg atgttccgct gatcggtttc gccggtgcgc cgtttacgct ggcgtcctac ctggtcgaag gcggtcctag tcgtaatcat ccccgcacta aggccatgat gctggccgaa cctgcgagct ggcatacgct gatggataag ctgaccgatc tgactcttgg atttttgctt ggtcaaatcg acgccggagt tgatgccatc caggtattcg actcgtgggc gggaacgctg tccctgtccg attaccgcca gtacgtgttg ccacatagcg cccggatttt tgccacggtg gccgaacacg gggtgccgat gacgcacttc ggggttggca ccgccgacct gctgggtgcg atgtcagcgg ctgtgcggtc aggcgaaaag cctgggcacc aggccgtcgt cggcgtggat tggcgaacct cgttgaccga tgctgctgcc cgagtggaac cctgtaccgc gctgcagggc aacctcgacc cggtggtgct gttagcgggc tggccggctg tcgaacgtgt cgcacggact gtcgtcgacg acgggcgccg tgccgtcgtg gccggcgcgg ctggccacgt tttcaatctc ggccatggag tgttgccgga gaccgatccc ggcgtgctga gcgaattagt atcgttcatc cactcgctaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a