MytuD.01068.a

phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: MytuD.01068.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01068.a.A1.GE25931 Full length( MytuD.01068.a ) 1 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01068.a.A1.PS00146 Full length( MytuD.01068.a ) 1 297

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.870
RefSeq: NP_215294.1
TubercuList: Rv0780
MTB Network Portal: Rv0780
UniProt: P9WHN1

Orthologues for Rv0780

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 88 99 1 1 0
marinum 87 100 1 1 0
ulcerans 87 100 0 0 0
avium 87 99 1 0 0
leprae 81 100 1 0 0
smegmatis 78 99 1 1 0
abscessus 76 100 1 1 1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPALSDYQH VASGKVREIY RVDDEHLLLV ASDRISAYDY VLDSTIPDKG RVLTAMSAFF FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RRLEMLPVEC VARGYLTGSG LLDYQATGKV CGIALPPGLV EASRFATPLF TPATKAALGD HDENISFDRV VEMVGALRAN QLRDRTLQTY VQAADHALTR GIIIADTKFE FGIDRHGNLL LADEIFTPDS SRYWPADDYR AGVVQTSFDK QFVRSWLTGS ESGWDRGSDR PPPPLPEHIV EATRARYINA YERISELKFD DWIGPGA
NT Sequence
ATGCGCCCCG CATTGTCCGA CTACCAGCAT GTGGCCAGCG GTAAGGTCCG CGAGATCTAC CGTGTCGATG ACGAGCACCT GCTGCTGGTT GCCAGCGACC GGATCTCGGC GTACGACTAC GTCCTGGACA GCACCATCCC GGACAAGGGC CGCGTCCTGA CCGCCATGAG CGCATTCTTC TTCGGGCTCG TCGATGCCCC TAACCATCTG GCCGGGCCGC CGGACGACCC GCGTATCCCC GACGAGGTGC TGGGCCGCGC GCTGGTGGTG CGTCGGCTGG AGATGCTGCC GGTGGAATGT GTGGCCCGTG GCTACCTGAC CGGTTCGGGG TTACTGGATT ACCAGGCAAC CGGGAAGGTA TGCGGTATCG CGCTGCCGCC GGGCCTGGTC GAGGCCAGTC GGTTCGCCAC ACCGCTGTTC ACCCCGGCGA CTAAAGCCGC GTTGGGGGAC CACGACGAGA ACATCTCGTT TGACCGGGTG GTGGAGATGG TAGGCGCGTT GCGTGCCAAC CAGCTGCGTG ATCGTACTCT GCAGACGTAT GTGCAGGCCG CCGATCACGC TCTCACCCGC GGAATCATTA TCGCCGACAC CAAGTTTGAA TTTGGCATCG ACCGCCACGG CAACCTGCTG CTGGCCGACG AAATCTTCAC ACCGGACTCG TCGCGGTACT GGCCTGCCGA CGACTACCGG GCCGGCGTGG TCCAGACCAG CTTCGACAAA CAGTTTGTCC GCAGCTGGCT CACCGGCTCC GAGTCCGGCT GGGATAGAGG CAGCGATCGG CCGCCGCCTC CGCTCCCCGA GCATATCGTC GAGGCCACGC GTGCCCGTTA TATTAATGCA TACGAACGGA TTTCCGAACT AAAATTCGAC GACTGGATCG GCCCTGGCGC A
Details for MytuD.01068.a.A1.GE25931
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HVASGKVREI YRVDDEHLLL VASDRISAYD YVLDSTIPDK GRVLTAMSAF FFGLVDAPNH LAGPPDDPRI PDEVLGRALV VRRLEMLPVE CVARGYLTGS GLLDYQATGK VCGIALPPGL VEASRFATPL FTPATKAALG DHDENISFDR VVEMVGALRA NQLRDRTLQT YVQAADHALT RGIIIADTKF EFGIDRHGNL LLADEIFTPD SSRYWPADDY RAGVVQTSFD KQFVRSWLTG SESGWDRGSD RPPPPLPEHI VEATRARYIN AYERISELKF DDWIGPGA
Validated NT Sequence
ttattatgcg ccagggccga tccagtcgtc gaattttagt tcggaaatcc gttcgtatgc attaatataa cgggcacgcg tggcctcgac gatatgctcg gggagcggag gcggcggccg atcgctgcct ctatcccagc cggactcgga gccggtgagc cagctgcgga caaactgttt gtcgaagctg gtctggacca cgccggcccg gtagtcgtcg gcaggccagt accgcgacga gtccggtgtg aagatttcgt cggccagcag caggttgccg tggcggtcga tgccaaattc aaacttggtg tcggcgataa tgattccgcg ggtgagagcg tgatcggcgg cctgcacata cgtctgcaga gtacgatcac gcagctggtt ggcacgcaac gcgcctacca tctccaccac ccggtcaaac gagatgttct cgtcgtggtc ccccaacgcg gctttagtcg ccggggtgaa cagcggtgtg gcgaaccgac tggcctcgac caggcccggc ggcagcgcga taccgcatac cttcccggtt gcctggtaat ccagtaaccc cgaaccggtc aggtagccac gggccacaca ttccaccggc agcatctcca gccgacgcac caccagcgcg cggcccagca cctcgtcggg gatacgcggg tcgtccggcg gcccggccag atggttaggg gcatcgacga gcccgaagaa gaatgcgctc atggcggtca ggacgcggcc cttgtccggg atggtgctgt ccaggacgta gtcgtacgcc gagatccggt cgctggcaac cagcagcagg tgctcgtcat cgacacggta gatctcgcgg accttaccgc tggccacatg ctggtagtcg gacaatgcgg ggcgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HVASGKVREI YRVDDEHLLL VASDRISAYD YVLDSTIPDK GRVLTAMSAF FFGLVDAPNH LAGPPDDPRI PDEVLGRALV VRRLEMLPVE CVARGYLTGS GLLDYQATGK VCGIALPPGL VEASRFATPL FTPATKAALG DHDENISFDR VVEMVGALRA NQLRDRTLQT YVQAADHALT RGIIIADTKF EFGIDRHGNL LLADEIFTPD SSRYWPADDY RAGVVQTSFD KQFVRSWLTG SESGWDRGSD RPPPPLPEHI VEATRARYIN AYERISELKF DDWIGPGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgccccgca ttgtccgact accagcatgt ggccagcggt aaggtccgcg agatctaccg tgtcgatgac gagcacctgc tgctggttgc cagcgaccgg atctcggcgt acgactacgt cctggacagc accatcccgg acaagggccg cgtcctgacc gccatgagcg cattcttctt cgggctcgtc gatgccccta accatctggc cgggccgccg gacgacccgc gtatccccga cgaggtgctg ggccgcgcgc tggtggtgcg tcggctggag atgctgccgg tggaatgtgt ggcccgtggc tacctgaccg gttcggggtt actggattac caggcaaccg ggaaggtatg cggtatcgcg ctgccgccgg gcctggtcga ggccagtcgg ttcgccacac cgctgttcac cccggcgact aaagccgcgt tgggggacca cgacgagaac atctcgtttg accgggtggt ggagatggta ggcgcgttgc gtgccaacca gctgcgtgat cgtactctgc agacgtatgt gcaggccgcc gatcacgctc tcacccgcgg aatcattatc gccgacacca agtttgaatt tggcatcgac cgccacggca acctgctgct ggccgacgaa atcttcacac cggactcgtc gcggtactgg cctgccgacg actaccgggc cggcgtggtc cagaccagct tcgacaaaca gtttgtccgc agctggctca ccggctccga gtccggctgg gatagaggca gcgatcggcc gccgcctccg ctccccgagc atatcgtcga ggccacgcgt gcccgttata ttaatgcata cgaacggatt tccgaactaa aattcgacga ctggatcggc cctggcgcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.01068.a.A1.PS00146
PURIFICATION DATe: 2/18/2009
CONCENTRATION: 36.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HVASGKVREI YRVDDEHLLL VASDRISAYD YVLDSTIPDK GRVLTAMSAF FFGLVDAPNH LAGPPDDPRI PDEVLGRALV VRRLEMLPVE CVARGYLTGS GLLDYQATGK VCGIALPPGL VEASRFATPL FTPATKAALG DHDENISFDR VVEMVGALRA NQLRDRTLQT YVQAADHALT RGIIIADTKF EFGIDRHGNL LLADEIFTPD SSRYWPADDY RAGVVQTSFD KQFVRSWLTG SESGWDRGSD RPPPPLPEHI VEATRARYIN AYERISELKF DDWIGPGA
Validated NT Sequence
ttattatgcg ccagggccga tccagtcgtc gaattttagt tcggaaatcc gttcgtatgc attaatataa cgggcacgcg tggcctcgac gatatgctcg gggagcggag gcggcggccg atcgctgcct ctatcccagc cggactcgga gccggtgagc cagctgcgga caaactgttt gtcgaagctg gtctggacca cgccggcccg gtagtcgtcg gcaggccagt accgcgacga gtccggtgtg aagatttcgt cggccagcag caggttgccg tggcggtcga tgccaaattc aaacttggtg tcggcgataa tgattccgcg ggtgagagcg tgatcggcgg cctgcacata cgtctgcaga gtacgatcac gcagctggtt ggcacgcaac gcgcctacca tctccaccac ccggtcaaac gagatgttct cgtcgtggtc ccccaacgcg gctttagtcg ccggggtgaa cagcggtgtg gcgaaccgac tggcctcgac caggcccggc ggcagcgcga taccgcatac cttcccggtt gcctggtaat ccagtaaccc cgaaccggtc aggtagccac gggccacaca ttccaccggc agcatctcca gccgacgcac caccagcgcg cggcccagca cctcgtcggg gatacgcggg tcgtccggcg gcccggccag atggttaggg gcatcgacga gcccgaagaa gaatgcgctc atggcggtca ggacgcggcc cttgtccggg atggtgctgt ccaggacgta gtcgtacgcc gagatccggt cgctggcaac cagcagcagg tgctcgtcat cgacacggta gatctcgcgg accttaccgc tggccacatg ctggtagtcg gacaatgcgg ggcgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HVASGKVREI YRVDDEHLLL VASDRISAYD YVLDSTIPDK GRVLTAMSAF FFGLVDAPNH LAGPPDDPRI PDEVLGRALV VRRLEMLPVE CVARGYLTGS GLLDYQATGK VCGIALPPGL VEASRFATPL FTPATKAALG DHDENISFDR VVEMVGALRA NQLRDRTLQT YVQAADHALT RGIIIADTKF EFGIDRHGNL LLADEIFTPD SSRYWPADDY RAGVVQTSFD KQFVRSWLTG SESGWDRGSD RPPPPLPEHI VEATRARYIN AYERISELKF DDWIGPGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCCCGCA TTGTCCGACT ACCAGCATGT GGCCAGCGGT AAGGTCCGCG AGATCTACCG TGTCGATGAC GAGCACCTGC TGCTGGTTGC CAGCGACCGG ATCTCGGCGT ACGACTACGT CCTGGACAGC ACCATCCCGG ACAAGGGCCG CGTCCTGACC GCCATGAGCG CATTCTTCTT CGGGCTCGTC GATGCCCCTA ACCATCTGGC CGGGCCGCCG GACGACCCGC GTATCCCCGA CGAGGTGCTG GGCCGCGCGC TGGTGGTGCG TCGGCTGGAG ATGCTGCCGG TGGAATGTGT GGCCCGTGGC TACCTGACCG GTTCGGGGTT ACTGGATTAC CAGGCAACCG GGAAGGTATG CGGTATCGCG CTGCCGCCGG GCCTGGTCGA GGCCAGTCGG TTCGCCACAC CGCTGTTCAC CCCGGCGACT AAAGCCGCGT TGGGGGACCA CGACGAGAAC ATCTCGTTTG ACCGGGTGGT GGAGATGGTA GGCGCGTTGC GTGCCAACCA GCTGCGTGAT CGTACTCTGC AGACGTATGT GCAGGCCGCC GATCACGCTC TCACCCGCGG AATCATTATC GCCGACACCA AGTTTGAATT TGGCATCGAC CGCCACGGCA ACCTGCTGCT GGCCGACGAA ATCTTCACAC CGGACTCGTC GCGGTACTGG CCTGCCGACG ACTACCGGGC CGGCGTGGTC CAGACCAGCT TCGACAAACA GTTTGTCCGC AGCTGGCTCA CCGGCTCCGA GTCCGGCTGG GATAGAGGCA GCGATCGGCC GCCGCCTCCG CTCCCCGAGC ATATCGTCGA GGCCACGCGT GCCCGTTATA TTAATGCATA CGAACGGATT TCCGAACTAA AATTCGACGA CTGGATCGGC CCTGGCGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A