MyabA.01068.a
Phosphoribosylaminoimidazole-succinocarboxamide synthase
| CENTER ID: | MyabA.01068.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01068.a.A1.GE30386 | Full length( MyabA.01068.a ) | 1 | 297 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01068.a.A1.PS00903 | Full length( MyabA.01068.a ) | 1 | 297 |
Structures
3R9R
DEPOSITED: 3/25/2011
DETERMINATION: XRay
CLONE: MyabA.01068.a.A1.GE30386
PROTEIN: MyabA.01068.a.A1.PS00903
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131443 |
| PATRIC ID: | fig|36809.5.peg.729 |
| RefSeq: | YP_001701440.1 |
| UniProt: | B1MHW4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPSLSDYQH VASGKVRELY RVDDEHLLFV ATDRISAFDF VLDTPIPDKG RILTAMSVFF FGLLTVPNHL AGPPDDPRIP EEVLGRALLV RRLDMLPVEC VARGYLTGSG LLDYQRTGAV CGHVLPQGLG EASRLDPPLF TPATKADIGE HDMNVDFAAV VGLVGAVRAN QLRDETIKIY TRAAAHALHK GIILADTKFE FGVDIEGNLV LADEVFTPDS SRYWDAAHYQ PGVVQDSFDK QFVRNWLTGP ESGWDRASDT PPPPLPDEVA VATRERYIEA YERISGLSFS DWIGPSA
NT Sequence
ATGCGTCCTT CGCTGTCCGA TTACCAGCAT GTGGCCAGCG GCAAGGTCCG TGAGCTGTAT CGCGTCGACG ACGAACACCT GCTGTTCGTT GCCACCGACC GGATTTCCGC ATTCGATTTC GTCCTGGATA CCCCGATACC CGATAAGGGA CGCATCCTCA CCGCGATGAG CGTGTTCTTC TTCGGACTGC TGACCGTGCC GAACCATCTG GCCGGTCCGC CCGACGATCC GCGCATTCCC GAGGAAGTGT TGGGCCGGGC ATTACTGGTG CGCCGCCTCG ACATGCTTCC CGTGGAATGT GTGGCACGCG GTTATCTCAC GGGGTCCGGG CTGCTGGATT ATCAGCGCAC CGGTGCGGTG TGTGGACATG TGTTGCCGCA GGGACTGGGG GAAGCCAGCC GCCTGGACCC ACCGTTGTTC ACCCCGGCGA CCAAAGCCGA CATCGGCGAG CACGATATGA ATGTCGACTT CGCCGCTGTG GTCGGTCTGG TCGGCGCTGT GCGTGCCAAC CAGCTGCGTG ACGAGACCAT CAAGATCTAC ACGCGCGCCG CGGCGCACGC CTTGCACAAG GGAATCATCC TGGCCGACAC CAAATTCGAA TTCGGCGTCG ATATCGAGGG CAATTTGGTG CTTGCCGACG AAGTGTTCAC GCCTGACTCG TCCCGGTACT GGGATGCCGC CCACTATCAG CCCGGGGTGG TTCAGGACAG CTTCGACAAG CAGTTCGTCC GCAATTGGCT TACCGGCCCC GAATCGGGAT GGGATCGTGC GTCGGATACT CCGCCGCCGC CGTTGCCAGA TGAGGTGGCA GTGGCCACGC GGGAACGTTA TATCGAGGCC TATGAGCGCA TTTCAGGGCT GAGCTTCTCT GATTGGATTG GCCCGTCGGC G
Details for MyabA.01068.a.A1.GE30386
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMRPSLSD YQHVASGKVR ELYRVDDEHL LFVATDRISA FDFVLDTPIP DKGRILTAMS VFFFGLLTVP NHLAGPPDDP RIPEEVLGRA LLVRRLDMLP VECVARGYLT GSGLLDYQRT GAVCGHVLPQ GLGEASRLDP PLFTPATKAD IGEHDMNVDF AAVVGLVGAV RANQLRDETI KIYTRAAAHA LHKGIILADT KFEFGVDIEG NLVLADEVFT PDSSRYWDAA HYQPGVVQDS FDKQFVRNWL TGPESGWDRA SDTPPPPLPD EVAVATRERY IEAYERISGL SFSDWIGPSA
Validated NT Sequence
ttattacgcc gacgggccaa tccaatcaga gaagctcagc cctgaaatgc gctcataggc ctcgatataa cgttcccgcg tggccactgc cacctcatct ggcaacggcg gcggcggagt atccgacgca cgatcccatc ccgattcggg gccggtaagc caattgcgga cgaactgctt gtcgaagctg tcctgaacca ccccgggctg atagtgggcg gcatcccagt accgggacga gtcaggcgtg aacacttcgt cggcaagcac caaattgccc tcgatatcga cgccgaattc gaatttggtg tcggccagga tgattccctt gtgcaaggcg tgcgccgcgg cgcgcgtgta gatcttgatg gtctcgtcac gcagctggtt ggcacgcaca gcgccgacca gaccgaccac agcggcgaag tcgacattca tatcgtgctc gccgatgtcg gctttggtcg ccggggtgaa caacggtggg tccaggcggc tggcttcccc cagtccctgc ggcaacacat gtccacacac cgcaccggtg cgctgataat ccagcagccc ggaccccgtg agataaccgc gtgccacaca ttccacggga agcatgtcga ggcggcgcac cagtaatgcc cggcccaaca cttcctcggg aatgcgcgga tcgtcgggcg gaccggccag atggttcggc acggtcagca gtccgaagaa gaacacgctc atcgcggtga ggatgcgtcc cttatcgggt atcggggtat ccaggacgaa atcgaatgcg gaaatccggt cggtggcaac gaacagcagg tgttcgtcgt cgacgcgata cagctcacgg accttgccgc tggccacatg ctggtaatcg gacagcgaag gacgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccan ggtatntc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPSLSDYQ HVASGKVREL YRVDDEHLLF VATDRISAFD FVLDTPIPDK GRILTAMSVF FFGLLTVPNH LAGPPDDPRI PEEVLGRALL VRRLDMLPVE CVARGYLTGS GLLDYQRTGA VCGHVLPQGL GEASRLDPPL FTPATKADIG EHDMNVDFAA VVGLVGAVRA NQLRDETIKI YTRAAAHALH KGIILADTKF EFGVDIEGNL VLADEVFTPD SSRYWDAAHY QPGVVQDSFD KQFVRNWLTG PESGWDRASD TPPPPLPDEV AVATRERYIE AYERISGLSF SDWIGPSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtccttcg ctgtccgatt accagcatgt ggccagcggc aaggtccgtg agctgtatcg cgtcgacgac gaacacctgc tgttcgttgc caccgaccgg atttccgcat tcgatttcgt cctggatacc ccgatacccg ataagggacg catcctcacc gcgatgagcg tgttcttctt cggactgctg accgtgccga accatctggc cggtccgccc gacgatccgc gcattcccga ggaagtgttg ggccgggcat tactggtgcg ccgcctcgac atgcttcccg tggaatgtgt ggcacgcggt tatctcacgg ggtccgggct gctggattat cagcgcaccg gtgcggtgtg tggacatgtg ttgccgcagg gactggggga agccagccgc ctggacccac cgttgttcac cccggcgacc aaagccgaca tcggcgagca cgatatgaat gtcgacttcg ccgctgtggt cggtctggtc ggcgctgtgc gtgccaacca gctgcgtgac gagaccatca agatctacac gcgcgccgcg gcgcacgcct tgcacaaggg aatcatcctg gccgacacca aattcgaatt cggcgtcgat atcgagggca atttggtgct tgccgacgaa gtgttcacgc ctgactcgtc ccggtactgg gatgccgccc actatcagcc cggggtggtt caggacagct tcgacaagca gttcgtccgc aattggctta ccggccccga atcgggatgg gatcgtgcgt cggatactcc gccgccgccg ttgccagatg aggtggcagt ggccacgcgg gaacgttata tcgaggccta tgagcgcatt tcagggctga gcttctctga ttggattggc ccgtcggcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.01068.a.A1.PS00903
| PURIFICATION DATe: | 12/7/2010 |
| CONCENTRATION: | 53.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMRPSLSD YQHVASGKVR ELYRVDDEHL LFVATDRISA FDFVLDTPIP DKGRILTAMS VFFFGLLTVP NHLAGPPDDP RIPEEVLGRA LLVRRLDMLP VECVARGYLT GSGLLDYQRT GAVCGHVLPQ GLGEASRLDP PLFTPATKAD IGEHDMNVDF AAVVGLVGAV RANQLRDETI KIYTRAAAHA LHKGIILADT KFEFGVDIEG NLVLADEVFT PDSSRYWDAA HYQPGVVQDS FDKQFVRNWL TGPESGWDRA SDTPPPPLPD EVAVATRERY IEAYERISGL SFSDWIGPSA
Validated NT Sequence
ttattacgcc gacgggccaa tccaatcaga gaagctcagc cctgaaatgc gctcataggc ctcgatataa cgttcccgcg tggccactgc cacctcatct ggcaacggcg gcggcggagt atccgacgca cgatcccatc ccgattcggg gccggtaagc caattgcgga cgaactgctt gtcgaagctg tcctgaacca ccccgggctg atagtgggcg gcatcccagt accgggacga gtcaggcgtg aacacttcgt cggcaagcac caaattgccc tcgatatcga cgccgaattc gaatttggtg tcggccagga tgattccctt gtgcaaggcg tgcgccgcgg cgcgcgtgta gatcttgatg gtctcgtcac gcagctggtt ggcacgcaca gcgccgacca gaccgaccac agcggcgaag tcgacattca tatcgtgctc gccgatgtcg gctttggtcg ccggggtgaa caacggtggg tccaggcggc tggcttcccc cagtccctgc ggcaacacat gtccacacac cgcaccggtg cgctgataat ccagcagccc ggaccccgtg agataaccgc gtgccacaca ttccacggga agcatgtcga ggcggcgcac cagtaatgcc cggcccaaca cttcctcggg aatgcgcgga tcgtcgggcg gaccggccag atggttcggc acggtcagca gtccgaagaa gaacacgctc atcgcggtga ggatgcgtcc cttatcgggt atcggggtat ccaggacgaa atcgaatgcg gaaatccggt cggtggcaac gaacagcagg tgttcgtcgt cgacgcgata cagctcacgg accttgccgc tggccacatg ctggtaatcg gacagcgaag gacgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccan ggtatntc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPSLSDYQ HVASGKVREL YRVDDEHLLF VATDRISAFD FVLDTPIPDK GRILTAMSVF FFGLLTVPNH LAGPPDDPRI PEEVLGRALL VRRLDMLPVE CVARGYLTGS GLLDYQRTGA VCGHVLPQGL GEASRLDPPL FTPATKADIG EHDMNVDFAA VVGLVGAVRA NQLRDETIKI YTRAAAHALH KGIILADTKF EFGVDIEGNL VLADEVFTPD SSRYWDAAHY QPGVVQDSFD KQFVRNWLTG PESGWDRASD TPPPPLPDEV AVATRERYIE AYERISGLSF SDWIGPSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTCCTTCG CTGTCCGATT ACCAGCATGT GGCCAGCGGC AAGGTCCGTG AGCTGTATCG CGTCGACGAC GAACACCTGC TGTTCGTTGC CACCGACCGG ATTTCCGCAT TCGATTTCGT CCTGGATACC CCGATACCCG ATAAGGGACG CATCCTCACC GCGATGAGCG TGTTCTTCTT CGGACTGCTG ACCGTGCCGA ACCATCTGGC CGGTCCGCCC GACGATCCGC GCATTCCCGA GGAAGTGTTG GGCCGGGCAT TACTGGTGCG CCGCCTCGAC ATGCTTCCCG TGGAATGTGT GGCACGCGGT TATCTCACGG GGTCCGGGCT GCTGGATTAT CAGCGCACCG GTGCGGTGTG TGGACATGTG TTGCCGCAGG GACTGGGGGA AGCCAGCCGC CTGGACCCAC CGTTGTTCAC CCCGGCGACC AAAGCCGACA TCGGCGAGCA CGATATGAAT GTCGACTTCG CCGCTGTGGT CGGTCTGGTC GGCGCTGTGC GTGCCAACCA GCTGCGTGAC GAGACCATCA AGATCTACAC GCGCGCCGCG GCGCACGCCT TGCACAAGGG AATCATCCTG GCCGACACCA AATTCGAATT CGGCGTCGAT ATCGAGGGCA ATTTGGTGCT TGCCGACGAA GTGTTCACGC CTGACTCGTC CCGGTACTGG GATGCCGCCC ACTATCAGCC CGGGGTGGTT CAGGACAGCT TCGACAAGCA GTTCGTCCGC AATTGGCTTA CCGGCCCCGA ATCGGGATGG GATCGTGCGT CGGATACTCC GCCGCCGCCG TTGCCAGATG AGGTGGCAGT GGCCACGCGG GAACGTTATA TCGAGGCCTA TGAGCGCATT TCAGGGCTGA GCTTCTCTGA TTGGATTGGC CCGTCGGCGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA