MyavA.01068.a

Phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: MyavA.01068.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01068.a.A1.GE29045 Full length( MyavA.01068.a ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.705
RefSeq: YP_880000.1
UniProt: A0A0H3A0J7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPALSDYQHL VSGKVRELYR VDDEHLLLVA TDRISAYDFV LDSTIPDKGR ILTAMSVFFF GLVDAPNHLA GPPDDPRIPD EVLGRALVVR QLEMLPVECV ARGYLTGSGL LDYQATGKVC GIALPPGLVE ASKFAEPLFT PATKAALGDH DENISFARVI EMVGGVRANQ LRDRTLQIYV QAADHALRKG IIIADTKFEF GTDPDGNLLL ADEIFTPDSS RYWPADEYRV GVPQTSFDKQ FVRNWLTSPE SGWDRHGTEP PPPLPDHIIE ATRNRYIEAY ERISGLRFDD WIGPRA
NT Sequence
ATGCCTGCAC TGTCGGACTA CCAGCATCTG GTCAGCGGCA AGGTCCGTGA GCTGTATCGC GTCGACGACG AGCACCTGCT GTTGGTCGCC ACCGACCGGA TCTCGGCGTA CGACTTCGTG CTGGACAGCA CCATCCCGGA CAAGGGCCGC ATCCTGACGG CGATGAGCGT CTTTTTCTTC GGCCTCGTCG ATGCCCCCAA CCACCTGGCC GGACCGCCCG ACGACCCGCG CATTCCCGAC GAGGTGCTGG GCCGCGCGCT GGTGGTGCGC CAGCTCGAGA TGCTCCCGGT GGAATGCGTG GCGCGCGGCT ACCTGACCGG CTCGGGGCTA CTGGACTACC AGGCGACCGG CAAGGTCTGC GGCATTGCGT TGCCGCCCGG ACTGGTCGAG GCGAGCAAGT TCGCCGAGCC GCTGTTCACC CCGGCGACCA AGGCCGCCCT GGGCGACCAC GACGAGAACA TCTCCTTTGC CCGGGTGATC GAGATGGTCG GCGGGGTGCG GGCCAACCAG CTGCGCGACC GCACGTTGCA GATCTACGTC CAGGCCGCCG ACCACGCGCT ACGGAAGGGA ATCATCATCG CGGACACCAA GTTCGAGTTC GGCACCGACC CGGATGGCAA TCTTCTGCTG GCCGACGAGA TCTTCACCCC GGACTCGTCG CGGTACTGGC CGGCCGACGA ATACCGGGTC GGCGTGCCAC AGACCAGCTT CGACAAACAG TTCGTCCGCA ACTGGCTCAC CAGCCCCGAA TCCGGCTGGG ACCGGCACGG CACCGAACCG CCCCCACCCC TGCCCGACCA CATCATCGAG GCCACCCGCA ACCGCTATAT CGAAGCCTAC GAACGCATTT CCGGGCTGCG TTTCGACGAC TGGATCGGTC CGCGCGCA
Details for MyavA.01068.a.A1.GE29045
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPALSDYQH LVSGKVRELY RVDDEHLLLV ATDRISAYDF VLDSTIPDKG RILTAMSVFF FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RQLEMLPVEC VARGYLTGSG LLDYQATGKV CGIALPPGLV EASKFAEPLF TPATKAALGD HDENISFARV IEMVGGVRAN QLRDRTLQIY VQAADHALRK GIIIADTKFE FGTDPDGNLL LADEIFTPDS SRYWPADEYR VGVPQTSFDK QFVRNWLTSP ESGWDRHGTE PPPPLPDHII EATRNRYIEA YERISGLRFD DWIGPRAN
Validated NT Sequence
ttattagcgc gcggaccgat ccagtcgtcg aaacgcagcc cggaaatgcg ttcgtaggct tcgatatagc ggttgcgggt ggcctcgatg atgtggtcgg gcagcggtgg gggcggttcg gtgccgtgcc ggtcccagcc ggattcgggg ctggtgagcc agttgcggac gaactgtttg tcgaagctgg tctgcggcac gccgacccgg tattcgtcgg ccggccagta ccgcgaggag tccggggtga agatctcgtc ggccagcaga agattgccat ccgggtcggt gccgaactcg aacttggtgt ccgcgatgat gattcccttc cgtagcgcgt ggtcggcggc ctggacgtag atctgcaacg tgcggtcgcg cagctggttg gcccgcaccc cgccgaccat ctcgatcacc cgggcaaagg agatgttctc gtcgtggtcg cccagggcgg ccttggtcgc cggggtgaac agcggctcgg cgaacttgct cgcctcgacc agtccgggcg gcaacgcaat gccgcagacc ttgccggtcg cctggtagtc cagtagcccc gagccggtca ggtagccgcg cgccacgcat tccaccggga gcatctcgag ctggcgcacc accagcgcgc ggcccagcac ctcgtcggga atgcgcgggt cgtcgggcgg tccggccagg tggttggggg catcgacgag gccgaagaaa aagacgctca tcgccgtcag gatgcggccc ttgtccggga tggtgctgtc cagcacgaag tcgtacgccg agatccggtc ggtggcgacc aacagcaggt gctcgtcgtc gacgcgatac agctcacgga ccttgccgct gaccagatgc tggtagtccg acagtgcagg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPALSDYQH LVSGKVRELY RVDDEHLLLV ATDRISAYDF VLDSTIPDKG RILTAMSVFF FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RQLEMLPVEC VARGYLTGSG LLDYQATGKV CGIALPPGLV EASKFAEPLF TPATKAALGD HDENISFARV IEMVGGVRAN QLRDRTLQIY VQAADHALRK GIIIADTKFE FGTDPDGNLL LADEIFTPDS SRYWPADEYR VGVPQTSFDK QFVRNWLTSP ESGWDRHGTE PPPPLPDHII EATRNRYIEA YERISGLRFD DWIGPRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgcactg tcggactacc agcatctggt cagcggcaag gtccgtgagc tgtatcgcgt cgacgacgag cacctgctgt tggtcgccac cgaccggatc tcggcgtacg acttcgtgct ggacagcacc atcccggaca agggccgcat cctgacggcg atgagcgtct ttttcttcgg cctcgtcgat gcccccaacc acctggccgg accgcccgac gacccgcgca ttcccgacga ggtgctgggc cgcgcgctgg tggtgcgcca gctcgagatg ctcccggtgg aatgcgtggc gcgcggctac ctgaccggct cggggctact ggactaccag gcgaccggca aggtctgcgg cattgcgttg ccgcccggac tggtcgaggc gagcaagttc gccgagccgc tgttcacccc ggcgaccaag gccgccctgg gcgaccacga cgagaacatc tcctttgccc gggtgatcga gatggtcggc ggggtgcggg ccaaccagct gcgcgaccgc acgttgcaga tctacgtcca ggccgccgac cacgcgctac ggaagggaat catcatcgcg gacaccaagt tcgagttcgg caccgacccg gatggcaatc ttctgctggc cgacgagatc ttcaccccgg actcgtcgcg gtactggccg gccgacgaat accgggtcgg cgtgccacag accagcttcg acaaacagtt cgtccgcaac tggctcacca gccccgaatc cggctgggac cggcacggca ccgaaccgcc cccacccctg cccgaccaca tcatcgaggc cacccgcaac cgctatatcg aagcctacga acgcatttcc gggctgcgtt tcgacgactg gatcggtccg cgcgcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa