SSGCID: Target Page

MypaA.01068.a

Phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID:	MypaA.01068.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01068.a.A1.GE29534	Full length( MypaA.01068.a )		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01068.a.A1.PS00750	Full length( MypaA.01068.a )		1	296

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.650
RefSeq:	NP_959548.1
UniProt:	Q743H1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPALSDYQHL ASGKVRELYR VDDEHLLLVA TDRISAYDFV LDSTIPDKGR ILTAMSVFFF GLVDAPNHLA GPPDDPRIPD EVLGRALVVR QLEMLPVECV ARGYLTGSGL LDYQATGKVC GIALPPGLVE ASKFAEPLFT PATKAALGDH DENISFARVI EMVGGVRANQ LRDRTLQIYV QAADHALRKG IIIADTKFEF GTDPDGNLLL ADEIFTPDSS RYWPADEYRV GVPQTSFDKQ FVRNWLTSPE SGWDRHGTEP PPPLPDHIIE ATRNRYIEAY ERISGLRFDD WIGPRA

NT Sequence

ATGCCTGCAC TGTCGGACTA CCAGCATCTG GCCAGCGGCA AGGTCCGTGA GCTGTATCGC GTCGACGACG AGCACCTGCT GTTGGTCGCC ACCGACCGGA TCTCGGCGTA CGACTTCGTG CTGGACAGCA CCATCCCGGA CAAGGGCCGC ATCCTGACGG CGATGAGCGT CTTTTTCTTC GGCCTCGTCG ATGCCCCCAA CCACCTGGCC GGACCGCCCG ACGACCCGCG CATTCCCGAC GAGGTGTTGG GCCGCGCGCT GGTGGTGCGC CAGCTCGAGA TGCTCCCGGT GGAATGCGTG GCGCGCGGCT ACCTGACAGG CTCGGGGCTA CTGGACTACC AGGCGACCGG CAAGGTCTGC GGCATCGCGT TGCCGCCCGG ACTGGTCGAG GCGAGCAAGT TCGCCGAGCC GCTGTTCACC CCGGCGACCA AGGCCGCCCT GGGCGACCAC GACGAGAACA TCTCTTTTGC CCGGGTGATC GAGATGGTCG GCGGGGTGCG GGCCAACCAG CTGCGCGACC GCACGTTGCA GATCTACGTC CAGGCCGCCG ACCACGCGCT ACGGAAGGGA ATCATCATCG CGGACACCAA GTTCGAGTTC GGCACCGACC CGGATGGCAA TCTTCTGCTG GCCGACGAGA TCTTCACCCC GGACTCCTCG CGGTACTGGC CGGCCGACGA ATACCGGGTC GGCGTGCCGC AGACCAGCTT CGACAAACAG TTCGTCCGCA ACTGGCTCAC CAGCCCCGAA TCCGGCTGGG ACCGGCACGG CACCGAACCG CCCCCACCGC TGCCCGACCA CATCATCGAG GCCACCCGCA ACCGCTATAT CGAAGCCTAC GAACGCATTT CCGGGCTGCG TTTCGACGAC TGGATCGGTC CGCGCGCA

Details for MypaA.01068.a.A1.GE29534

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPALSDYQH LASGKVRELY RVDDEHLLLV ATDRISAYDF VLDSTIPDKG RILTAMSVFF FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RQLEMLPVEC VARGYLTGSG LLDYQATGKV CGIALPPGLV EASKFAEPLF TPATKAALGD HDENISFARV IEMVGGVRAN QLRDRTLQIY VQAADHALRK GIIIADTKFE FGTDPDGNLL LADEIFTPDS SRYWPADEYR VGVPQTSFDK QFVRNWLTSP ESGWDRHGTE PPPPLPDHII EATRNRYIEA YERISGLRFD DWIGPRA

Validated NT Sequence

tattatgcgc gcggaccgat ccagtcgtcg aaacgcagcc cggaaatgcg ttcgtaggct tcgatatagc ggttgcgggt ggcctcgatg atgtggtcgg gcagcggtgg gggcggttcg gtgccgtgcc ggtcccagcc ggattcgggg ctggtgagcc agttgcggac gaactgtttg tcgaagctgg tctgcggcac gccgacccgg tattcgtcgg ccggccagta ccgcgaggag tccggggtga agatctcgtc ggccagcaga agattgccat ccgggtcggt gccgaactcg aacttggtgt ccgcgatgat gattcccttc cgtagcgcgt ggtcggcggc ctggacgtag atctgcaacg tgcggtcgcg cagctggttg gcccgcaccc cgccgaccat ctcgatcacc cgggcaaaag agatgttctc gtcgtggtcg cccagggcgg ccttggtcgc cggggtgaac agcggctcgg cgaacttgct cgcctcgacc agtccgggcg gcaacgcgat gccgcagacc ttgccggtcg cctggtagtc cagtagcccc gagcctgtca ggtagccgcg cgccacgcat tccaccggga gcatctcgag ctggcgcacc accagcgcgc ggcccaacac ctcgtcggga atgcgcgggt cgtcgggcgg tccggccagg tggttggggg catcgacgag gccgaagaaa aagacgctca tcgccgtcag gatgcggccc ttgtccggga tggtgctgtc cagcacgaag tcgtacgccg agatccggtc ggtggcgacc aacagcaggt gctcgtcgtc gacgcgatac agctcacgga ccttgccgct ggccagatgc tggtagtccg acagtgcagg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tntctccttc ttaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgcactg tcggactacc agcatctggc cagcggcaag gtccgtgagc tgtatcgcgt cgacgacgag cacctgctgt tggtcgccac cgaccggatc tcggcgtacg acttcgtgct ggacagcacc atcccggaca agggccgcat cctgacggcg atgagcgtct ttttcttcgg cctcgtcgat gcccccaacc acctggccgg accgcccgac gacccgcgca ttcccgacga ggtgttgggc cgcgcgctgg tggtgcgcca gctcgagatg ctcccggtgg aatgcgtggc gcgcggctac ctgacaggct cggggctact ggactaccag gcgaccggca aggtctgcgg catcgcgttg ccgcccggac tggtcgaggc gagcaagttc gccgagccgc tgttcacccc ggcgaccaag gccgccctgg gcgaccacga cgagaacatc tcttttgccc gggtgatcga gatggtcggc ggggtgcggg ccaaccagct gcgcgaccgc acgttgcaga tctacgtcca ggccgccgac cacgcgctac ggaagggaat catcatcgcg gacaccaagt tcgagttcgg caccgacccg gatggcaatc ttctgctggc cgacgagatc ttcaccccgg actcctcgcg gtactggccg gccgacgaat accgggtcgg cgtgccgcag accagcttcg acaaacagtt cgtccgcaac tggctcacca gccccgaatc cggctgggac cggcacggca ccgaaccgcc cccaccgctg cccgaccaca tcatcgaggc cacccgcaac cgctatatcg aagcctacga acgcatttcc gggctgcgtt tcgacgactg gatcggtccg cgcgcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MypaA.01068.a.A1.PS00750

PURIFICATION DATe:	9/2/2010
CONCENTRATION:	29.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTGCACTG TCGGACTACC AGCATCTGGC CAGCGGCAAG GTCCGTGAGC TGTATCGCGT CGACGACGAG CACCTGCTGT TGGTCGCCAC CGACCGGATC TCGGCGTACG ACTTCGTGCT GGACAGCACC ATCCCGGACA AGGGCCGCAT CCTGACGGCG ATGAGCGTCT TTTTCTTCGG CCTCGTCGAT GCCCCCAACC ACCTGGCCGG ACCGCCCGAC GACCCGCGCA TTCCCGACGA GGTGTTGGGC CGCGCGCTGG TGGTGCGCCA GCTCGAGATG CTCCCGGTGG AATGCGTGGC GCGCGGCTAC CTGACAGGCT CGGGGCTACT GGACTACCAG GCGACCGGCA AGGTCTGCGG CATCGCGTTG CCGCCCGGAC TGGTCGAGGC GAGCAAGTTC GCCGAGCCGC TGTTCACCCC GGCGACCAAG GCCGCCCTGG GCGACCACGA CGAGAACATC TCTTTTGCCC GGGTGATCGA GATGGTCGGC GGGGTGCGGG CCAACCAGCT GCGCGACCGC ACGTTGCAGA TCTACGTCCA GGCCGCCGAC CACGCGCTAC GGAAGGGAAT CATCATCGCG GACACCAAGT TCGAGTTCGG CACCGACCCG GATGGCAATC TTCTGCTGGC CGACGAGATC TTCACCCCGG ACTCCTCGCG GTACTGGCCG GCCGACGAAT ACCGGGTCGG CGTGCCGCAG ACCAGCTTCG ACAAACAGTT CGTCCGCAAC TGGCTCACCA GCCCCGAATC CGGCTGGGAC CGGCACGGCA CCGAACCGCC CCCACCGCTG CCCGACCACA TCATCGAGGC CACCCGCAAC CGCTATATCG AAGCCTACGA ACGCATTTCC GGGCTGCGTT TCGACGACTG GATCGGTCCG CGCGCAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA