MyleA.01068.a

Phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: MyleA.01068.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01068.a.A1.GE29247 Full length( MyleA.01068.a ) 1 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4246
RefSeq: YP_002504086.1
UniProt: B8ZSW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPALFDYRH LASGKVREIY RIDDEHLLMV ASDRISAFDY VLDSTIPDKG RILTAMSVFF FSFVDAPNHL AGPPDDPRIP EEVLGRALVV RQLDMLPMEC VARGYLTGSG LLDYQATGKV CGIPLPPGLV EASRFAAPLF TPAMKAVLGE HDENISFSRM IEMVGVVRAN QLRDRTLQIY LQAADHALKT GIIIADTKFE FGIDRDGNLL LADEIFTPDS SRYWSVDDYR VGVVQRSFDK QFVRNWLVSP ESGWVRAGAL SPPSLPDDII EATRLCYVEV YERISGMSFD DWIGPGA
NT Sequence
ATGCGTCCCG CATTGTTCGA CTACCGGCAT TTGGCCAGCG GCAAGGTCCG CGAGATATAC CGCATCGACG ACGAGCACCT CTTAATGGTT GCGTCGGATC GGATATCGGC GTTCGACTAC GTTCTGGACA GCACGATTCC GGACAAAGGC CGGATCCTGA CCGCGATGAG TGTGTTTTTC TTTAGCTTCG TAGACGCTCC CAACCACCTG GCCGGGCCGC CCGACGACCC GCGCATTCCC GAGGAGGTGC TTGGCCGCGC GCTGGTGGTT CGGCAGCTGG ACATGCTGCC GATGGAGTGT GTAGCTCGCG GTTACCTGAC CGGTTCGGGA CTATTGGATT ATCAGGCGAC CGGGAAGGTT TGCGGCATCC CGTTGCCGCC AGGCCTGGTC GAAGCCAGCA GGTTCGCCGC TCCGCTTTTC ACCCCGGCTA TGAAAGCTGT TCTAGGAGAA CATGATGAGA ACATTTCGTT TTCCAGGATG ATTGAGATGG TGGGTGTGGT GCGTGCCAAC CAGCTACGCG ACCGGACTTT GCAGATTTAC CTTCAGGCAG CTGATCACGC ACTGAAGACC GGAATAATCA TAGCTGACAC TAAGTTTGAA TTTGGTATCG ACCGCGACGG CAACCTGCTT CTGGCCGATG AGATTTTCAC ACCGGACTCC TCGCGGTACT GGTCAGTCGA CGACTACCGG GTCGGTGTGG TTCAGAGAAG CTTCGACAAG CAGTTCGTCC GTAACTGGCT TGTCAGTCCT GAGTCGGGTT GGGTCCGCGC TGGAGCATTG TCCCCGCCGT CGCTACCGGA TGACATTATC GAGGCCACCC GTCTATGCTA TGTCGAAGTT TATGAACGGA TTTCCGGTAT GAGCTTCGAC GATTGGATCG GTCCAGGAGC A
Details for MyleA.01068.a.A1.GE29247
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRPALFD YRHLASGKVR EIYRIDDEHL LMVASDRISA FDYVLDSTIP DKGRILTAMS VFFFSFVDAP NHLAGPPDDP RIPEEVLGRA LVVRQLDMLP MECVARGYLT GSGLLDYQAT GKVCGIPLPP GLVEASRFAA PLFTPAMKAV LGEHDENISF SRMIEMVGVV RANQLRDRTL QIYLQAADHA LKTGIIIADT KFEFGIDRDG NLLLADEIFT PDSSRYWSVD DYRVGVVQRS FDKQFVRNWL VSPESGWVRA GALSPPSLPD DIIEATRLCY VEVYERISGM SFDDWIGPGA
Validated NT Sequence
atgctcctgg accgatccaa tcgtcgaagc tcataccgga aatccgttca taaacttcga catagcatag acgggtggcc tcgataatgt catccggtag cgacggcggg gacaatgctc cagcgcggac ccaacccgac tcaggactga caagccagtt acggacgaac tgcttgtcga agcttctctg aaccacaccg acccggtagt cgtcgactga ccagtaccgc gaggagtccg gtgtgaaaat ctcatcggcc agaagcaggt tgccgtcgcg gtcgatacca aattcaaact tagtgtcagc tatgattatt ccggtcttca gtgcgtgatc agctgcctga aggtaaatct gcaaagtccg gtcgcgtagc tggttggcac gcaccacacc caccatctca atcatcctgg aaaacgaaat gttctcatca tgttctccta gaacagcttt catagccggg gtgaaaagcg gagcggcgaa cctgctggct tcgaccaggc ctggcggcaa cgggatgccg caaaccttcc cggtcgcctg ataatccaat agtcccgaac cggtcaggta accgcgagct acacactcca tcggcagcat gtccagctgc cgaaccacca gcgcgcggcc aagcacctcc tcgggaatgc gcgggtcgtc gggcggcccg gccaggtggt tgggagcgtc tacgaagcta aagaaaaaca cactcatcgc ggtcaggatc cggcctttgt ccggaatcgt gctgtccaga acgtagtcga acgccgatat ccgatccgac gcaaccatta agaggtgctc gtcgtcgatg cggtatatct cgcggacctt gccgctggcc aaatgccggt agtcgaacaa tgcgggacgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALFDYR HLASGKVREI YRIDDEHLLM VASDRISAFD YVLDSTIPDK GRILTAMSVF FFSFVDAPNH LAGPPDDPRI PEEVLGRALV VRQLDMLPME CVARGYLTGS GLLDYQATGK VCGIPLPPGL VEASRFAAPL FTPAMKAVLG EHDENISFSR MIEMVGVVRA NQLRDRTLQI YLQAADHALK TGIIIADTKF EFGIDRDGNL LLADEIFTPD SSRYWSVDDY RVGVVQRSFD KQFVRNWLVS PESGWVRAGA LSPPSLPDDI IEATRLCYVE VYERISGMSF DDWIGPGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtcccgca ttgttcgact accggcattt ggccagcggc aaggtccgcg agatataccg catcgacgac gagcacctct taatggttgc gtcggatcgg atatcggcgt tcgactacgt tctggacagc acgattccgg acaaaggccg gatcctgacc gcgatgagtg tgtttttctt tagcttcgta gacgctccca accacctggc cgggccgccc gacgacccgc gcattcccga ggaggtgctt ggccgcgcgc tggtggttcg gcagctggac atgctgccga tggagtgtgt agctcgcggt tacctgaccg gttcgggact attggattat caggcgaccg ggaaggtttg cggcatcccg ttgccgccag gcctggtcga agccagcagg ttcgccgctc cgcttttcac cccggctatg aaagctgttc taggagaaca tgatgagaac atttcgtttt ccaggatgat tgagatggtg ggtgtggtgc gtgccaacca gctacgcgac cggactttgc agatttacct tcaggcagct gatcacgcac tgaagaccgg aataatcata gctgacacta agtttgaatt tggtatcgac cgcgacggca acctgcttct ggccgatgag attttcacac cggactcctc gcggtactgg tcagtcgacg actaccgggt cggtgtggtt cagagaagct tcgacaagca gttcgtccgt aactggcttg tcagtcctga gtcgggttgg gtccgcgctg gagcattgtc cccgccgtcg ctaccggatg acattatcga ggccacccgt ctatgctatg tcgaagttta tgaacggatt tccggtatga gcttcgacga ttggatcggt ccaggagcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a