MysmA.01068.a

Phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: MysmA.01068.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01068.a.A1.GE28864 Full length( MysmA.01068.a ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01068.a.A1.PW28769 Full length( MysmA.01068.a ) 1 299

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5685
RefSeq: YP_890068.1
UniProt: A0R4I0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPALSDYQH LASGKVREIY RIDDEHLLFV ASDRISAYDY ILDSQIPDKG RILTAMSVFF FDHLLRTAGV PNHLAGPPDD ERIPADVLGR ALVVRRLDML PVECVARGYL TGSGLIDYEK TGTVCGIALP PGLGEASKFD EPLFTPATKA EIGEHDENIS FAKVIELVGA ELANQLRDRT LQTYTAGADH ALSKGIIIAD TKFEFGVDRD GTVVLADEVF TPDSSRYWRA DSYQPGVVQN SFDKQFVRNW LTGPESGWDR HGNTPPPALP DDIVAATRER YIEAYERISG LSFDDWIGA
NT Sequence
ATGCGCCCTG CTCTGTCCGA CTACCAACAC CTGGCCAGCG GCAAAGTCCG CGAGATCTAC CGCATCGACG ACGAGCACCT GCTCTTCGTG GCCAGCGACC GGATTTCGGC CTATGACTAC ATCCTGGATT CCCAGATCCC GGACAAGGGC CGGATCCTGA CCGCGATGAG CGTGTTCTTC TTCGACCACC TGCTGCGAAC GGCGGGTGTG CCCAACCACC TCGCAGGCCC GCCCGACGAC GAGCGCATCC CCGCCGACGT GCTGGGCCGC GCGCTCGTGG TGCGCCGCCT GGACATGCTG CCGGTCGAGT GCGTGGCGCG CGGCTATCTG ACCGGCTCGG GATTGATCGA TTACGAGAAG ACGGGCACGG TGTGCGGCAT CGCGCTGCCG CCCGGGCTGG GGGAGGCGAG CAAGTTCGAC GAGCCGTTGT TCACCCCGGC CACCAAGGCC GAGATCGGCG AGCACGACGA GAACATCTCG TTCGCGAAGG TGATCGAGCT GGTGGGCGCG GAGCTGGCCA ACCAGCTGCG CGACCGCACG CTGCAGACCT ACACCGCCGG CGCCGACCAT GCGCTGAGCA AGGGGATCAT CATCGCCGAC ACCAAGTTCG AGTTCGGTGT CGACAGGGAC GGCACCGTGG TGCTGGCCGA CGAGGTGTTC ACGCCCGACT CGTCGCGCTA CTGGCGCGCC GACAGCTACC AGCCGGGCGT GGTGCAGAAC AGCTTCGACA AGCAGTTCGT ACGCAACTGG CTCACCGGCC CGGAGTCGGG CTGGGACCGC CACGGCAACA CCCCGCCACC TGCGCTGCCC GACGACATCG TCGCGGCCAC GCGCGAGCGG TACATCGAGG CCTACGAACG CATCTCGGGT CTCAGTTTCG ACGATTGGAT CGGTGCA
Details for MysmA.01068.a.A1.GE28864
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HLASGKVREI YRIDDEHLLF VASDRISAYD YILDSQIPDK GRILTAMSVF FFDHLLRTAG VPNHLAGPPD DERIPADVLG RALVVRRLDM LPVECVARGY LTGSGLIDYE KTGTVCGIAL PPGLGEASKF DEPLFTPATK AEIGEHDENI SFAKVIELVG AELANQLRDR TLQTYTAGAD HALSKGIIIA DTKFEFGVDR DGTVVLADEV FTPDSSRYWR ADSYQPGVVQ NSFDKQFVRN WLTGPESGWD RHGNTPPPAL PDDIVAATRE RYIEAYERIS GLSFDDWIGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc ctgctctgtc cgactaccaa cacctggcca gcggcaaagt ccgcgagatc taccgcatcg acgacgagca cctgctcttc gtggccagcg accggatttc ggcctatgac tacatcctgg attcccagat cccggacaag ggccggatcc tgaccgcgat gagcgtgttc ttcttcgacc acctgctgcg aacggcgggt gtgcccaacc acctcgcagg cccgcccgac gacgagcgca tccccgccga cgtgctgggc cgcgcgctcg tggtgcgccg cctggacatg ctgccggtcg agtgcgtggc gcgcggctat ctgaccggct cgggattgat cgattacgag aagacgggca cggtgtgcgg catcgcgctg ccgcccgggc tgggggaggc gagcaagttc gacgagccgt tgttcacccc ggccaccaag gccgagatcg gcgagcacga cgagaacatc tcgttcgcga aggtgatcga gctggtgggc gcggagctgg ccaaccagct gcgcgaccgc acgctgcaga cctacaccgc cggcgccgac catgcgctga gcaaggggat catcatcgcc gacaccaagt tcgagttcgg tgtcgacagg gacggcaccg tggtgctggc cgacgaggtg ttcacgcccg actcgtcgcg ctactggcgc gccgacagct accagccggg cgtggtgcag aacagcttcg acaagcagtt cgtacgcaac tggctcaccg gcccggagtc gggctgggac cgccacggca acaccccgcc acctgcgctg cccgacgaca tcgtcgcggc cacgcgcgag cggtacatcg aggcctacga acgcatctcg ggtctcagtt tcgacgattg gatcggtgca taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HLASGKVREI YRIDDEHLLF VASDRISAYD YILDSQIPDK GRILTAMSVF FFDHLLRTAG VPNHLAGPPD DERIPADVLG RALVVRRLDM LPVECVARGY LTGSGLIDYE KTGTVCGIAL PPGLGEASKF DEPLFTPATK AEIGEHDENI SFAKVIELVG AELANQLRDR TLQTYTAGAD HALSKGIIIA DTKFEFGVDR DGTVVLADEV FTPDSSRYWR ADSYQPGVVQ NSFDKQFVRN WLTGPESGWD RHGNTPPPAL PDDIVAATRE RYIEAYERIS GLSFDDWIGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgccctgct ctgtccgact accaacacct ggccagcggc aaagtccgcg agatctaccg catcgacgac gagcacctgc tcttcgtggc cagcgaccgg atttcggcct atgactacat cctggattcc cagatcccgg acaagggccg gatcctgacc gcgatgagcg tgttcttctt cgaccacctg ctgcgaacgg cgggtgtgcc caaccacctc gcaggcccgc ccgacgacga gcgcatcccc gccgacgtgc tgggccgcgc gctcgtggtg cgccgcctgg acatgctgcc ggtcgagtgc gtggcgcgcg gctatctgac cggctcggga ttgatcgatt acgagaagac gggcacggtg tgcggcatcg cgctgccgcc cgggctgggg gaggcgagca agttcgacga gccgttgttc accccggcca ccaaggccga gatcggcgag cacgacgaga acatctcgtt cgcgaaggtg atcgagctgg tgggcgcgga gctggccaac cagctgcgcg accgcacgct gcagacctac accgccggcg ccgaccatgc gctgagcaag gggatcatca tcgccgacac caagttcgag ttcggtgtcg acagggacgg caccgtggtg ctggccgacg aggtgttcac gcccgactcg tcgcgctact ggcgcgccga cagctaccag ccgggcgtgg tgcagaacag cttcgacaag cagttcgtac gcaactggct caccggcccg gagtcgggct gggaccgcca cggcaacacc ccgccacctg cgctgcccga cgacatcgtc gcggccacgc gcgagcggta catcgaggcc tacgaacgca tctcgggtct cagtttcgac gattggatcg gtgcaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.01068.a.A1.PW28769
PURIFICATION DATe: 5/17/2010
CONCENTRATION: 24.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HLASGKVREI YRIDDEHLLF VASDRISAYD YILDSQIPDK GRILTAMSVF FFDHLLRTAG VPNHLAGPPD DERIPADVLG RALVVRRLDM LPVECVARGY LTGSGLIDYE KTGTVCGIAL PPGLGEASKF DEPLFTPATK AEIGEHDENI SFAKVIELVG AELANQLRDR TLQTYTAGAD HALSKGIIIA DTKFEFGVDR DGTVVLADEV FTPDSSRYWR ADSYQPGVVQ NSFDKQFVRN WLTGPESGWD RHGNTPPPAL PDDIVAATRE RYIEAYERIS GLSFDDWIGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcc ctgctctgtc cgactaccaa cacctggcca gcggcaaagt ccgcgagatc taccgcatcg acgacgagca cctgctcttc gtggccagcg accggatttc ggcctatgac tacatcctgg attcccagat cccggacaag ggccggatcc tgaccgcgat gagcgtgttc ttcttcgacc acctgctgcg aacggcgggt gtgcccaacc acctcgcagg cccgcccgac gacgagcgca tccccgccga cgtgctgggc cgcgcgctcg tggtgcgccg cctggacatg ctgccggtcg agtgcgtggc gcgcggctat ctgaccggct cgggattgat cgattacgag aagacgggca cggtgtgcgg catcgcgctg ccgcccgggc tgggggaggc gagcaagttc gacgagccgt tgttcacccc ggccaccaag gccgagatcg gcgagcacga cgagaacatc tcgttcgcga aggtgatcga gctggtgggc gcggagctgg ccaaccagct gcgcgaccgc acgctgcaga cctacaccgc cggcgccgac catgcgctga gcaaggggat catcatcgcc gacaccaagt tcgagttcgg tgtcgacagg gacggcaccg tggtgctggc cgacgaggtg ttcacgcccg actcgtcgcg ctactggcgc gccgacagct accagccggg cgtggtgcag aacagcttcg acaagcagtt cgtacgcaac tggctcaccg gcccggagtc gggctgggac cgccacggca acaccccgcc acctgcgctg cccgacgaca tcgtcgcggc cacgcgcgag cggtacatcg aggcctacga acgcatctcg ggtctcagtt tcgacgattg gatcggtgca taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPALSDYQ HLASGKVREI YRIDDEHLLF VASDRISAYD YILDSQIPDK GRILTAMSVF FFDHLLRTAG VPNHLAGPPD DERIPADVLG RALVVRRLDM LPVECVARGY LTGSGLIDYE KTGTVCGIAL PPGLGEASKF DEPLFTPATK AEIGEHDENI SFAKVIELVG AELANQLRDR TLQTYTAGAD HALSKGIIIA DTKFEFGVDR DGTVVLADEV FTPDSSRYWR ADSYQPGVVQ NSFDKQFVRN WLTGPESGWD RHGNTPPPAL PDDIVAATRE RYIEAYERIS GLSFDDWIGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCCTGCT CTGTCCGACT ACCAACACCT GGCCAGCGGC AAAGTCCGCG AGATCTACCG CATCGACGAC GAGCACCTGC TCTTCGTGGC CAGCGACCGG ATTTCGGCCT ATGACTACAT CCTGGATTCC CAGATCCCGG ACAAGGGCCG GATCCTGACC GCGATGAGCG TGTTCTTCTT CGACCACCTG CTGCGAACGG CGGGTGTGCC CAACCACCTC GCAGGCCCGC CCGACGACGA GCGCATCCCC GCCGACGTGC TGGGCCGCGC GCTCGTGGTG CGCCGCCTGG ACATGCTGCC GGTCGAGTGC GTGGCGCGCG GCTATCTGAC CGGCTCGGGA TTGATCGATT ACGAGAAGAC GGGCACGGTG TGCGGCATCG CGCTGCCGCC CGGGCTGGGG GAGGCGAGCA AGTTCGACGA GCCGTTGTTC ACCCCGGCCA CCAAGGCCGA GATCGGCGAG CACGACGAGA ACATCTCGTT CGCGAAGGTG ATCGAGCTGG TGGGCGCGGA GCTGGCCAAC CAGCTGCGCG ACCGCACGCT GCAGACCTAC ACCGCCGGCG CCGACCATGC GCTGAGCAAG GGGATCATCA TCGCCGACAC CAAGTTCGAG TTCGGTGTCG ACAGGGACGG CACCGTGGTG CTGGCCGACG AGGTGTTCAC GCCCGACTCG TCGCGCTACT GGCGCGCCGA CAGCTACCAG CCGGGCGTGG TGCAGAACAG CTTCGACAAG CAGTTCGTAC GCAACTGGCT CACCGGCCCG GAGTCGGGCT GGGACCGCCA CGGCAACACC CCGCCACCTG CGCTGCCCGA CGACATCGTC GCGGCCACGC GCGAGCGGTA CATCGAGGCC TACGAACGCA TCTCGGGTCT CAGTTTCGAC GATTGGATCG GTGCAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA