MytuD.00441.a

PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT (BETA SUBUNIT) PDHB (PYRUVATE DECARBOXYLASE) (PYRUVATE DEHYDROGENASE) (PYRUVIC DEHYDROGENASE)

CENTER ID: MytuD.00441.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00441.a.B1.GE26989 Full length( MytuD.00441.a ) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2798
RefSeq: NP_217012.1
TubercuList: Rv2496c
MTB Network Portal: Rv2496c
UniProt: P9WIS1

Orthologues for Rv2496c

Species % Identity % Coverage Clones Proteins Structures
marinum 86 100 1 0 0
ulcerans 85 100 1 0 0
avium 84 100 0 0 0
paratuberculosis 84 100 1 0 0
smegmatis 74 100 0 0 0
abscessus 57 91 1 0 0
abscessus 55 92 1 0 0
thermoresistibile 38 93 1 2 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQIADRPAR PDETLAVAVS DITQSLTMVQ AINRALYDAM AADERVLVFG EDVAVEGGVF RVTEGLADTF GADRCFDTPL AESAIIGIAV GLALRGFVPV PEIQFDGFSY PAFDQVVSHL AKYRTRTRGE VDMPVTVRIP SFGGIGAAEH HSDSTESYWV HTAGLKVVVP STPGDAYWLL RHAIACPDPV MYLEPKRRYH GRGMVDTSRP EPPIGHAMVR RSGTDVTVVT YGNLVSTALS SADTAEQQHD WSLEVIDLRS LAPLDFDTIA ASIQRTGRCV VMHEGPRSLG YGAGLAARIQ EEMFYQLEAP VLRACGFDTP YPPARLEKLW LPGPDRLLDC VERVLRQP
NT Sequence
ATGACTCAAA TCGCAGACCG TCCGGCCAGG CCGGATGAGA CGCTTGCGGT AGCGGTGAGC GACATCACGC AGTCGTTGAC CATGGTCCAG GCGATCAACC GCGCCCTGTA CGACGCGATG GCCGCCGACG AGCGGGTATT GGTGTTTGGC GAGGATGTCG CGGTCGAGGG TGGGGTGTTT CGGGTGACCG AGGGACTGGC CGATACGTTC GGTGCAGACC GGTGCTTCGA CACGCCACTG GCGGAGTCCG CGATCATCGG AATAGCCGTC GGGTTGGCAC TACGTGGCTT TGTGCCGGTG CCAGAAATCC AGTTCGACGG CTTTTCCTAT CCGGCTTTCG ACCAAGTGGT GAGCCATCTG GCCAAGTACC GGACTCGCAC CCGCGGTGAG GTGGACATGC CGGTGACCGT CCGGATCCCC TCGTTTGGCG GTATCGGTGC GGCCGAACAT CATTCGGACT CCACGGAGTC CTACTGGGTG CACACCGCGG GCCTCAAGGT GGTGGTGCCG TCGACACCCG GTGACGCTTA CTGGCTGCTG CGGCACGCTA TCGCCTGCCC GGACCCCGTG ATGTATCTGG AGCCCAAACG CCGCTACCAC GGCCGCGGGA TGGTCGACAC TAGCCGGCCG GAACCGCCGA TCGGGCACGC GATGGTGCGC CGGTCCGGCA CCGATGTGAC CGTGGTAACG TACGGAAACC TGGTGAGCAC CGCCCTGTCT AGCGCGGATA CTGCAGAGCA GCAACATGAT TGGAGTCTGG AGGTTATCGA TCTGCGGTCG TTGGCGCCGC TGGACTTCGA CACCATTGCT GCGTCGATCC AGCGCACCGG GCGTTGCGTG GTGATGCACG AAGGGCCGCG CAGCCTCGGC TATGGTGCCG GTCTGGCCGC GCGCATCCAG GAAGAGATGT TCTACCAATT GGAGGCTCCG GTGTTGCGTG CCTGCGGTTT TGACACACCC TACCCGCCTG CGCGGTTGGA AAAATTGTGG TTACCCGGCC CCGACCGGCT GTTGGACTGC GTCGAACGTG TGCTGCGTCA GCCA
Details for MytuD.00441.a.B1.GE26989
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MTQIADRPAR PDETLAVAVS DITQSLTMVQ AINRALYDAM AADERVLVFG EDVAVEGGVF RVTEGLADTF GADRCFDTPL AESAIIGIAV GLALRGFVPV PEIQFDGFSY PAFDQVVSHL AKYRTRTRGE VDMPVTVRIP SFGGIGAAEH HSDSTESYWV HTAGLKVVVP STPGDAYWLL RHAIACPDPV MYLEPKRRYH GRGMVDTSRP EPPIGHAMVR RSGTDVTVVT YGNLVSTALS SADTAEQQHD WSLEVIDLRS LAPLDFDTIA ASIQRTGRCV VMHEGPRSLG YGAGLAARIQ EEMFYQLEAP VLRACGFDTP YPPARLEKLW LPGPDRLLDC VERVLRQP
Validated NT Sequence
tatgactcaa atcgcagacc gtccggccag gccggatgag acgcttgcgg tagcggtgag cgacatcacg cagtcgttga ccatggtcca ggcgatcaac cgcgccctgt acgacgcgat ggccgccgac gagcgggtat tggtgtttgg cgaggatgtc gcggtcgagg gtggggtgtt tcgggtgacc gagggactgg ccgatacgtt cggtgcagac cggtgcttcg acacgccact ggcggagtcc gcgatcatcg gaatagccgt cgggttggca ctacgtggct ttgtgccggt gccagaaatc cagttcgacg gcttttccta tccggctttc gaccaagtgg tgagccatct ggccaagtac cggactcgca cccgcggtga ggtggacatg ccggtgaccg tccggatccc ctcgtttggc ggtatcggtg cggccgaaca tcattcggac tccacggagt cctactgggt gcacaccgcg ggcctcaagg tggtggtgcc gtcgacaccc ggtgacgctt actggctgct gcggcacgct atcgcctgcc cggaccccgt gatgtatctg gagcccaaac gccgctacca cggccgcggg atggtcgaca ctagccggcc ggaaccgccg atcgggcacg cgatggtgcg ccggtccggc accgatgtga ccgtggtaac gtacggaaac ctggtgagca ccgccctgtc tagcgcggat actgcagagc agcaacatga ttggagtctg gaggttatcg atctgcggtc gttggcgccg ctggacttcg acaccattgc tgcgtcgatc cagcgcaccg ggcgttgcgt ggtgatgcac gaagggccgc gcagcctcgg ctatggtgcc ggtctggccg cgcgcatcca ggaagagatg ttctaccaat tggaggctcc ggtgttgcgt gcctgcggtt ttgacacacc ctacccgcct gcgcggttgg aaaaattgtg gttacccggc cccgaccggc tgttggactg cgtcgaacgt gtgctgcgtc agccataagt gagta
Expected Protein Sequence
MAHHHHHHMT QIADRPARPD ETLAVAVSDI TQSLTMVQAI NRALYDAMAA DERVLVFGED VAVEGGVFRV TEGLADTFGA DRCFDTPLAE SAIIGIAVGL ALRGFVPVPE IQFDGFSYPA FDQVVSHLAK YRTRTRGEVD MPVTVRIPSF GGIGAAEHHS DSTESYWVHT AGLKVVVPST PGDAYWLLRH AIACPDPVMY LEPKRRYHGR GMVDTSRPEP PIGHAMVRRS GTDVTVVTYG NLVSTALSSA DTAEQQHDWS LEVIDLRSLA PLDFDTIAAS IQRTGRCVVM HEGPRSLGYG AGLAARIQEE MFYQLEAPVL RACGFDTPYP PARLEKLWLP GPDRLLDCVE RVLRQP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactc aaatcgcaga ccgtccggcc aggccggatg agacgcttgc ggtagcggtg agcgacatca cgcagtcgtt gaccatggtc caggcgatca accgcgccct gtacgacgcg atggccgccg acgagcgggt attggtgttt ggcgaggatg tcgcggtcga gggtggggtg tttcgggtga ccgagggact ggccgatacg ttcggtgcag accggtgctt cgacacgcca ctggcggagt ccgcgatcat cggaatagcc gtcgggttgg cactacgtgg ctttgtgccg gtgccagaaa tccagttcga cggcttttcc tatccggctt tcgaccaagt ggtgagccat ctggccaagt accggactcg cacccgcggt gaggtggaca tgccggtgac cgtccggatc ccctcgtttg gcggtatcgg tgcggccgaa catcattcgg actccacgga gtcctactgg gtgcacaccg cgggcctcaa ggtggtggtg ccgtcgacac ccggtgacgc ttactggctg ctgcggcacg ctatcgcctg cccggacccc gtgatgtatc tggagcccaa acgccgctac cacggccgcg ggatggtcga cactagccgg ccggaaccgc cgatcgggca cgcgatggtg cgccggtccg gcaccgatgt gaccgtggta acgtacggaa acctggtgag caccgccctg tctagcgcgg atactgcaga gcagcaacat gattggagtc tggaggttat cgatctgcgg tcgttggcgc cgctggactt cgacaccatt gctgcgtcga tccagcgcac cgggcgttgc gtggtgatgc acgaagggcc gcgcagcctc ggctatggtg ccggtctggc cgcgcgcatc caggaagaga tgttctacca attggaggct ccggtgttgc gtgcctgcgg ttttgacaca ccctacccgc ctgcgcggtt ggaaaaattg tggttacccg gccccgaccg gctgttggac tgcgtcgaac gtgtgctgcg tcagccatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at