MypaA.00441.a

PdhB

CENTER ID: MypaA.00441.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00441.a.A1.GE29452 Full length( MypaA.00441.a ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2457
RefSeq: NP_961242.1
UniProt: Q73XK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQLADRPTG HGETLTAAVQ DVALHAQALT MVQALNRALH DAMAADERVL VFGEDVAVQG GVFRVTEGLA EAFGESRCFD TPLAESAIIG IAVGLALRGF VPVPEIQFDG FSYPAFDQVV SHLAKYRTRT RGAIDMPVTV RVPSFGGIGA AEHHSESTES YWAHTAGLKV VVPSNPADAY WLLRHAIACP DPVMYLEPKR RYQGRGLVDA GRPEPPIGRA MVRRAGTDVT VVTYGSLVGT AVGAAEEAQR QRGWSLEVID LRSLVPLDFD TIATSIHRTG RCVVMHEGPR TLGYGAELAA RIQEELFYEL EAPVLRACGF DTPYPPARLE KWWLPGPDRL LDCVERTLEL P
NT Sequence
ATGACCCAGC TCGCTGATCG CCCGACCGGC CACGGCGAAA CCCTCACCGC GGCAGTGCAG GATGTCGCGC TGCACGCGCA GGCGTTGACC ATGGTGCAGG CGCTCAACCG CGCCCTGCAC GACGCGATGG CCGCCGACGA GCGGGTGCTG GTGTTCGGTG AAGACGTCGC GGTCCAGGGC GGTGTCTTCC GGGTGACCGA AGGGCTGGCG GAGGCGTTCG GCGAGAGTCG TTGCTTCGAT ACGCCGTTGG CGGAATCCGC GATCATCGGC ATCGCGGTGG GGCTGGCGCT GCGCGGCTTC GTGCCGGTGC CGGAGATCCA GTTCGACGGG TTCTCCTACC CGGCGTTCGA CCAAGTGGTC AGCCATCTGG CGAAGTACCG CACCCGGACC CGCGGCGCGA TCGACATGCC GGTGACGGTC CGGGTACCGT CGTTCGGCGG AATCGGTGCC GCCGAACATC ATTCGGAATC CACCGAGTCG TACTGGGCGC ACACCGCCGG CCTGAAGGTG GTGGTGCCGT CCAACCCGGC CGACGCGTAC TGGCTGCTGC GCCACGCCAT CGCCTGCCCG GACCCGGTGA TGTACCTGGA GCCCAAGCGT CGCTACCAAG GCCGCGGCCT GGTGGACGCC GGCCGGCCCG AACCGCCGAT CGGGCGGGCG ATGGTGCGCC GGGCGGGGAC CGACGTGACC GTCGTGACCT ACGGCAGTCT GGTCGGCACG GCGGTCGGAG CCGCCGAAGA GGCTCAGCGC CAACGCGGTT GGAGCCTGGA GGTCATCGAC CTGCGGTCGT TGGTGCCGCT GGACTTCGAC ACCATCGCGA CATCCATCCA CCGGACCGGT CGGTGCGTGG TGATGCACGA GGGGCCGCGG ACCCTGGGTT ACGGAGCCGA GCTGGCCGCC CGCATCCAGG AGGAGTTGTT CTACGAACTG GAGGCGCCGG TGTTGCGCGC GTGCGGATTC GACACCCCCT ACCCGCCGGC CCGGCTGGAG AAGTGGTGGC TGCCGGGCCC GGACCGGCTG CTGGACTGCG TCGAGCGCAC GTTGGAGCTG CCG
Details for MypaA.00441.a.A1.GE29452
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQLADRPT GHGETLTAAV QDVALHAQAL TMVQALNRAL HDAMAADERV LVFGEDVAVQ GGVFRVTEGL AEAFGESRCF DTPLAESAII GIAVGLALRG FVPVPEIQFD GFSYPAFDQV VSHLAKYRTR TRGAIDMPVT VRVPSFGGIG AAEHHSESTE SYWAHTAGLK VVVPSNPADA YWLLRHAIAC PDPVMYLEPK RRYQGRGLVD AGRPEPPIGR AMVRRAGTDV TVVTYGSLVG TAVGAAEXAQ RQRGWXLEVI DLRXLVPLDF XTXATSXXRT GRXVVXHXGX RXLGXXPXWX PXXXXXXXXX XXRXCXXXXX XXXPXPXXXX XXXXARPXXX XXXXXX
Validated NT Sequence
annnttttnt ttaactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg acccagctcg ctgatcgccc gaccggccac ggcgaaaccc tcaccgcggc agtgcaggat gtcgcgctgc acgcgcaggc gttgaccatg gtgcaggcgc tcaaccgcgc cctgcacgac gcgatggccg ccgacgagcg ggtgctggtg ttcggtgaag acgtcgcggt ccagggcggt gtcttccggg tgaccgaagg gctggcggag gcgttcggcg agagtcgttg cttcgatacg ccgttggcgg aatccgcgat catcggcatc gcggtggggc tggcgctgcg cggcttcgtg ccggtgccgg agatccagtt cgacgggttc tcctacccgg cgttcgacca agtggtcagc catctggcga agtaccgcac ccggacccgc ggcgcgatcg acatgccggt gacggtccgg gtaccgtcgt tcggcggaat cggtgccgcc gaacatcatt cggaatccac cgagtcgtac tgggcgcaca ccgccggcct gaaggtggtg gtgccgtcca acccggccga cgcgtactgg ctgctgcgcc acgccatcgc ctgcccggac ccggtgatgt acctggagcc caagcgtcgc taccaaggcc gcggcctggt ggacgccggc cggcccgaac cgccgatcgg gcgggcgatg gtgcgccggg cggggaccga cgtgaccgtc gtgacctacg gcagtctggt cggcacggcg gtcggagccg ccgaanangc tcagcgccaa cgcggttgga gnctggaggt catcgacctg cggncgttgg tgccgctgga cttcnacacc ntcgcgacat cnntncnccg gaccggncgg ngcgtggtga ngcacnangg gncgcgnanc ctgggtnacg anccnanntg gnngcccgnn tncnnngnan ttnttcnnnc nnnntgnngg cgnngntgtt gnncgcnngn ngnnttnnnn nncccccnac cnnncnnnnn nnnnnngnnn nnnncggccc gnccnnnnnn ntgnnntnnn tnnncnnnnn nnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQLADRPT GHGETLTAAV QDVALHAQAL TMVQALNRAL HDAMAADERV LVFGEDVAVQ GGVFRVTEGL AEAFGESRCF DTPLAESAII GIAVGLALRG FVPVPEIQFD GFSYPAFDQV VSHLAKYRTR TRGAIDMPVT VRVPSFGGIG AAEHHSESTE SYWAHTAGLK VVVPSNPADA YWLLRHAIAC PDPVMYLEPK RRYQGRGLVD AGRPEPPIGR AMVRRAGTDV TVVTYGSLVG TAVGAAEEAQ RQRGWSLEVI DLRSLVPLDF DTIATSIHRT GRCVVMHEGP RTLGYGAELA ARIQEELFYE LEAPVLRACG FDTPYPPARL EKWWLPGPDR LLDCVERTLE LP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccagctc gctgatcgcc cgaccggcca cggcgaaacc ctcaccgcgg cagtgcagga tgtcgcgctg cacgcgcagg cgttgaccat ggtgcaggcg ctcaaccgcg ccctgcacga cgcgatggcc gccgacgagc gggtgctggt gttcggtgaa gacgtcgcgg tccagggcgg tgtcttccgg gtgaccgaag ggctggcgga ggcgttcggc gagagtcgtt gcttcgatac gccgttggcg gaatccgcga tcatcggcat cgcggtgggg ctggcgctgc gcggcttcgt gccggtgccg gagatccagt tcgacgggtt ctcctacccg gcgttcgacc aagtggtcag ccatctggcg aagtaccgca cccggacccg cggcgcgatc gacatgccgg tgacggtccg ggtaccgtcg ttcggcggaa tcggtgccgc cgaacatcat tcggaatcca ccgagtcgta ctgggcgcac accgccggcc tgaaggtggt ggtgccgtcc aacccggccg acgcgtactg gctgctgcgc cacgccatcg cctgcccgga cccggtgatg tacctggagc ccaagcgtcg ctaccaaggc cgcggcctgg tggacgccgg ccggcccgaa ccgccgatcg ggcgggcgat ggtgcgccgg gcggggaccg acgtgaccgt cgtgacctac ggcagtctgg tcggcacggc ggtcggagcc gccgaagagg ctcagcgcca acgcggttgg agcctggagg tcatcgacct gcggtcgttg gtgccgctgg acttcgacac catcgcgaca tccatccacc ggaccggtcg gtgcgtggtg atgcacgagg ggccgcggac cctgggttac ggagccgagc tggccgcccg catccaggag gagttgttct acgaactgga ggcgccggtg ttgcgcgcgt gcggattcga caccccctac ccgccggccc ggctggagaa gtggtggctg ccgggcccgg accggctgct ggactgcgtc gagcgcacgt tggagctgcc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa