MythA.00441.a

Pyruvate dehydrogenase E1 component subunit beta (A0R1D3 homolog)

CENTER ID: MythA.00441.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00441.a.A1.GE28735 Full length( MythA.00441.a ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00441.a.A1.PS00629 Full length( MythA.00441.a ) 1 353
MythA.00441.a.A1.PS00630 Full length( MythA.00441.a ) 1 353

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1812
RefSeq: WP_003925337.1
UniProt: G7CFS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTGNGTELA DGTVPRVALP EGETEELGLV QAIHRTLDRA MAADDSIILL GEDIGDRSGG GMFKITAGLS AKYGEERVRD TPIAESSIIG AALGASLAGL RPVAELMFMD FLGVAMDQIA NHVAKVRYMS GGRRGAPLVI RTMVGTAAGA QHSQALEAWA MHTPGLXXXX XXXXXXXXXX XXXXXXXXXX XEDDPCLFIE SMKLYYGGGK GPVPVADYVI PLGQADVKRV GSDVTICTYG VLVHAALEAA KTLAGEGVSV EVVDLRTLVP LDLQTVLDSV RKTKRLVVAH ESVGFCGPGA EIAAAVGTEL FAELAAPIQR VAGTYTPVPR AATLEAACRP DATKLSDAVR RIM
NT Sequence
GTGTTCACGG GCAACGGGAC CGAGTTGGCC GACGGCACCG TCCCGCGGGT CGCGCTGCCC GAGGGGGAGA CCGAGGAACT CGGGCTGGTG CAGGCGATCC ACCGCACGCT GGACCGGGCG ATGGCCGCCG ACGATTCGAT CATCCTGCTG GGCGAGGACA TCGGTGACCG CTCCGGTGGC GGCATGTTCA AGATCACCGC GGGACTGTCG GCCAAGTACG GCGAAGAGCG GGTCCGCGAC ACCCCGATCG CGGAGTCGTC GATCATCGGC GCCGCGCTGG GGGCCTCGCT GGCAGGTCTG CGGCCCGTCG CCGAGTTGAT GTTCATGGAT TTTCTCGGGG TGGCGATGGA CCAGATCGCC AACCATGTCG CCAAGGTGCG CTACATGTCG GGCGGGCGCC GCGGCGCGCC GCTGGTGATC CGCACGATGG TGGGGACCGC CGCCGGGGCG CAGCACTCCC AGGCTCTCGA GGCATGGGCC ATGCACACCC CGGGACTCAN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGAGGACG ACCCGTGCCT GTTCATCGAA TCGATGAAGC TGTACTACGG CGGCGGCAAG GGGCCGGTTC CGGTAGCCGA CTACGTGATC CCGCTCGGCC AGGCCGATGT CAAGCGGGTC GGCAGCGACG TCACCATCTG CACCTACGGC GTGTTGGTGC ATGCCGCGCT GGAAGCAGCG AAAACCCTTG CGGGCGAGGG TGTCTCTGTC GAGGTAGTCG ATCTGAGGAC GTTGGTTCCG CTGGACCTGC AGACCGTGCT CGACTCGGTG CGCAAGACCA AGCGCCTGGT CGTCGCACAC GAATCGGTCG GCTTCTGCGG TCCCGGTGCC GAGATCGCCG CAGCCGTCGG TACCGAACTG TTCGCAGAGC TTGCGGCGCC GATTCAACGC GTCGCGGGCA CCTACACCCC GGTGCCGAGG GCCGCCACGC TGGAGGCGGC CTGCCGCCCA GATGCCACAA AGCTCAGTGA TGCCGTCAGG AGGATCATG
Details for MythA.00441.a.A1.GE28735
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 94
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLKV VWPSTAADAA GLLNACLRED DPCLFIESMK LYYGGGKGPV PVADYVIPLG QADVKRVGSD VTICTYGVLV HAALEAAKTL AGEGVSVEVV DLRTLVPLDL QTVLDSVRKT KRLVVAHESV GFCGPGAEIA AAVGTELFAE LAAPIQRVAG TYTPVPRAAT LEAACRPDAT KLSDAVRRIM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tcacgggcaa cgggaccgag ttggccgacg gcaccgtccc gcgggtcgcg ctgcccgagg gggagaccga ggaactcggg ctggtgcagg cgatccaccg cacgctggac cgggcgatgg ccgccgacga ttcgatcatc ctgctgggcg aggacatcgg tgaccgctcc ggtggcggca tgttcaagat caccgcggga ctgtcggcca agtacggcga agagcgggtc cgcgacaccc cgatcgcgga gtcgtcgatc atcggcgccg cgctgggggc ctcgctggca ggtctgcggc ccgtcgccga gttgatgttc atggattttc tcggggtggc gatggaccag atcgccaacc atgtcgccaa ggtgcgctac atgtcgggcg ggcgccgcgg cgcgccgctg gtgatccgca cgatggtggg gaccgccgcc ggggcgcagc actcccaggc tctcgaggca tgggccatgc acaccccggg actcaaggtc gtctggccca gcaccgccgc agatgccgcc ggcctgctca acgcgtgcct gcgtgaggac gacccgtgcc tgttcatcga atcgatgaag ctgtactacg gcggcggcaa ggggccggtt ccggtagccg actacgtgat cccgctcggc caggccgatg tcaagcgggt cggcagcgac gtcaccatct gcacctacgg cgtgttggtg catgccgcgc tggaagcagc gaaaaccctt gcgggcgagg gtgtctctgt cgaggtagtc gatctgagga cgttggttcc gctggacctg cagaccgtgc tcgactcggt gcgcaagacc aagcgcctgg tcgtcgcaca cgaatcggtc ggcttctgcg gtcccggtgc cgagatcgcc gcagccgtcg gtaccgaact gttcgcagag cttgcggcgc cgattcaacg cgtcgcgggc acctacaccc cggtgccgag ggccgccacg ctggaggcgg cctgccgccc agatgccaca aagctcagtg atgccgtcag gaggatcatg taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLXX XXXXXXXXXX XXXXXXXXXX XXXEDDPCLF IESMKLYYGG GKGPVPVADY VIPLGQADVK RVGSDVTICT YGVLVHAALE AAKTLAGEGV SVEVVDLRTL VPLDLQTVLD SVRKTKRLVV AHESVGFCGP GAEIAAAVGT ELFAELAAPI QRVAGTYTPV PRAATLEAAC RPDATKLSDA VRRIM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttcacg ggcaacggga ccgagttggc cgacggcacc gtcccgcggg tcgcgctgcc cgagggggag accgaggaac tcgggctggt gcaggcgatc caccgcacgc tggaccgggc gatggccgcc gacgattcga tcatcctgct gggcgaggac atcggtgacc gctccggtgg cggcatgttc aagatcaccg cgggactgtc ggccaagtac ggcgaagagc gggtccgcga caccccgatc gcggagtcgt cgatcatcgg cgccgcgctg ggggcctcgc tggcaggtct gcggcccgtc gccgagttga tgttcatgga ttttctcggg gtggcgatgg accagatcgc caaccatgtc gccaaggtgc gctacatgtc gggcgggcgc cgcggcgcgc cgctggtgat ccgcacgatg gtggggaccg ccgccggggc gcagcactcc caggctctcg aggcatgggc catgcacacc ccgggactca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaggac gacccgtgcc tgttcatcga atcgatgaag ctgtactacg gcggcggcaa ggggccggtt ccggtagccg actacgtgat cccgctcggc caggccgatg tcaagcgggt cggcagcgac gtcaccatct gcacctacgg cgtgttggtg catgccgcgc tggaagcagc gaaaaccctt gcgggcgagg gtgtctctgt cgaggtagtc gatctgagga cgttggttcc gctggacctg cagaccgtgc tcgactcggt gcgcaagacc aagcgcctgg tcgtcgcaca cgaatcggtc ggcttctgcg gtcccggtgc cgagatcgcc gcagccgtcg gtaccgaact gttcgcagag cttgcggcgc cgattcaacg cgtcgcgggc acctacaccc cggtgccgag ggccgccacg ctggaggcgg cctgccgccc agatgccaca aagctcagtg atgccgtcag gaggatcatg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MythA.00441.a.A1.PS00629
PURIFICATION DATe: 6/7/2010
CONCENTRATION: 38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLKV VWPSTAADAA GLLNACLRED DPCLFIESMK LYYGGGKGPV PVADYVIPLG QADVKRVGSD VTICTYGVLV HAALEAAKTL AGEGVSVEVV DLRTLVPLDL QTVLDSVRKT KRLVVAHESV GFCGPGAEIA AAVGTELFAE LAAPIQRVAG TYTPVPRAAT LEAACRPDAT KLSDAVRRIM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tcacgggcaa cgggaccgag ttggccgacg gcaccgtccc gcgggtcgcg ctgcccgagg gggagaccga ggaactcggg ctggtgcagg cgatccaccg cacgctggac cgggcgatgg ccgccgacga ttcgatcatc ctgctgggcg aggacatcgg tgaccgctcc ggtggcggca tgttcaagat caccgcggga ctgtcggcca agtacggcga agagcgggtc cgcgacaccc cgatcgcgga gtcgtcgatc atcggcgccg cgctgggggc ctcgctggca ggtctgcggc ccgtcgccga gttgatgttc atggattttc tcggggtggc gatggaccag atcgccaacc atgtcgccaa ggtgcgctac atgtcgggcg ggcgccgcgg cgcgccgctg gtgatccgca cgatggtggg gaccgccgcc ggggcgcagc actcccaggc tctcgaggca tgggccatgc acaccccggg actcaaggtc gtctggccca gcaccgccgc agatgccgcc ggcctgctca acgcgtgcct gcgtgaggac gacccgtgcc tgttcatcga atcgatgaag ctgtactacg gcggcggcaa ggggccggtt ccggtagccg actacgtgat cccgctcggc caggccgatg tcaagcgggt cggcagcgac gtcaccatct gcacctacgg cgtgttggtg catgccgcgc tggaagcagc gaaaaccctt gcgggcgagg gtgtctctgt cgaggtagtc gatctgagga cgttggttcc gctggacctg cagaccgtgc tcgactcggt gcgcaagacc aagcgcctgg tcgtcgcaca cgaatcggtc ggcttctgcg gtcccggtgc cgagatcgcc gcagccgtcg gtaccgaact gttcgcagag cttgcggcgc cgattcaacg cgtcgcgggc acctacaccc cggtgccgag ggccgccacg ctggaggcgg cctgccgccc agatgccaca aagctcagtg atgccgtcag gaggatcatg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLXX XXXXXXXXXX XXXXXXXXXX XXXEDDPCLF IESMKLYYGG GKGPVPVADY VIPLGQADVK RVGSDVTICT YGVLVHAALE AAKTLAGEGV SVEVVDLRTL VPLDLQTVLD SVRKTKRLVV AHESVGFCGP GAEIAAAVGT ELFAELAAPI QRVAGTYTPV PRAATLEAAC RPDATKLSDA VRRIM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTCACG GGCAACGGGA CCGAGTTGGC CGACGGCACC GTCCCGCGGG TCGCGCTGCC CGAGGGGGAG ACCGAGGAAC TCGGGCTGGT GCAGGCGATC CACCGCACGC TGGACCGGGC GATGGCCGCC GACGATTCGA TCATCCTGCT GGGCGAGGAC ATCGGTGACC GCTCCGGTGG CGGCATGTTC AAGATCACCG CGGGACTGTC GGCCAAGTAC GGCGAAGAGC GGGTCCGCGA CACCCCGATC GCGGAGTCGT CGATCATCGG CGCCGCGCTG GGGGCCTCGC TGGCAGGTCT GCGGCCCGTC GCCGAGTTGA TGTTCATGGA TTTTCTCGGG GTGGCGATGG ACCAGATCGC CAACCATGTC GCCAAGGTGC GCTACATGTC GGGCGGGCGC CGCGGCGCGC CGCTGGTGAT CCGCACGATG GTGGGGACCG CCGCCGGGGC GCAGCACTCC CAGGCTCTCG AGGCATGGGC CATGCACACC CCGGGACTCA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGAGGAC GACCCGTGCC TGTTCATCGA ATCGATGAAG CTGTACTACG GCGGCGGCAA GGGGCCGGTT CCGGTAGCCG ACTACGTGAT CCCGCTCGGC CAGGCCGATG TCAAGCGGGT CGGCAGCGAC GTCACCATCT GCACCTACGG CGTGTTGGTG CATGCCGCGC TGGAAGCAGC GAAAACCCTT GCGGGCGAGG GTGTCTCTGT CGAGGTAGTC GATCTGAGGA CGTTGGTTCC GCTGGACCTG CAGACCGTGC TCGACTCGGT GCGCAAGACC AAGCGCCTGG TCGTCGCACA CGAATCGGTC GGCTTCTGCG GTCCCGGTGC CGAGATCGCC GCAGCCGTCG GTACCGAACT GTTCGCAGAG CTTGCGGCGC CGATTCAACG CGTCGCGGGC ACCTACACCC CGGTGCCGAG GGCCGCCACG CTGGAGGCGG CCTGCCGCCC AGATGCCACA AAGCTCAGTG ATGCCGTCAG GAGGATCATG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for MythA.00441.a.A1.PS00630
PURIFICATION DATe: 6/7/2010
CONCENTRATION: 42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLKV VWPSTAADAA GLLNACLRED DPCLFIESMK LYYGGGKGPV PVADYVIPLG QADVKRVGSD VTICTYGVLV HAALEAAKTL AGEGVSVEVV DLRTLVPLDL QTVLDSVRKT KRLVVAHESV GFCGPGAEIA AAVGTELFAE LAAPIQRVAG TYTPVPRAAT LEAACRPDAT KLSDAVRRIM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tcacgggcaa cgggaccgag ttggccgacg gcaccgtccc gcgggtcgcg ctgcccgagg gggagaccga ggaactcggg ctggtgcagg cgatccaccg cacgctggac cgggcgatgg ccgccgacga ttcgatcatc ctgctgggcg aggacatcgg tgaccgctcc ggtggcggca tgttcaagat caccgcggga ctgtcggcca agtacggcga agagcgggtc cgcgacaccc cgatcgcgga gtcgtcgatc atcggcgccg cgctgggggc ctcgctggca ggtctgcggc ccgtcgccga gttgatgttc atggattttc tcggggtggc gatggaccag atcgccaacc atgtcgccaa ggtgcgctac atgtcgggcg ggcgccgcgg cgcgccgctg gtgatccgca cgatggtggg gaccgccgcc ggggcgcagc actcccaggc tctcgaggca tgggccatgc acaccccggg actcaaggtc gtctggccca gcaccgccgc agatgccgcc ggcctgctca acgcgtgcct gcgtgaggac gacccgtgcc tgttcatcga atcgatgaag ctgtactacg gcggcggcaa ggggccggtt ccggtagccg actacgtgat cccgctcggc caggccgatg tcaagcgggt cggcagcgac gtcaccatct gcacctacgg cgtgttggtg catgccgcgc tggaagcagc gaaaaccctt gcgggcgagg gtgtctctgt cgaggtagtc gatctgagga cgttggttcc gctggacctg cagaccgtgc tcgactcggt gcgcaagacc aagcgcctgg tcgtcgcaca cgaatcggtc ggcttctgcg gtcccggtgc cgagatcgcc gcagccgtcg gtaccgaact gttcgcagag cttgcggcgc cgattcaacg cgtcgcgggc acctacaccc cggtgccgag ggccgccacg ctggaggcgg cctgccgccc agatgccaca aagctcagtg atgccgtcag gaggatcatg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFTGNGTE LADGTVPRVA LPEGETEELG LVQAIHRTLD RAMAADDSII LLGEDIGDRS GGGMFKITAG LSAKYGEERV RDTPIAESSI IGAALGASLA GLRPVAELMF MDFLGVAMDQ IANHVAKVRY MSGGRRGAPL VIRTMVGTAA GAQHSQALEA WAMHTPGLXX XXXXXXXXXX XXXXXXXXXX XXXEDDPCLF IESMKLYYGG GKGPVPVADY VIPLGQADVK RVGSDVTICT YGVLVHAALE AAKTLAGEGV SVEVVDLRTL VPLDLQTVLD SVRKTKRLVV AHESVGFCGP GAEIAAAVGT ELFAELAAPI QRVAGTYTPV PRAATLEAAC RPDATKLSDA VRRIM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTCACG GGCAACGGGA CCGAGTTGGC CGACGGCACC GTCCCGCGGG TCGCGCTGCC CGAGGGGGAG ACCGAGGAAC TCGGGCTGGT GCAGGCGATC CACCGCACGC TGGACCGGGC GATGGCCGCC GACGATTCGA TCATCCTGCT GGGCGAGGAC ATCGGTGACC GCTCCGGTGG CGGCATGTTC AAGATCACCG CGGGACTGTC GGCCAAGTAC GGCGAAGAGC GGGTCCGCGA CACCCCGATC GCGGAGTCGT CGATCATCGG CGCCGCGCTG GGGGCCTCGC TGGCAGGTCT GCGGCCCGTC GCCGAGTTGA TGTTCATGGA TTTTCTCGGG GTGGCGATGG ACCAGATCGC CAACCATGTC GCCAAGGTGC GCTACATGTC GGGCGGGCGC CGCGGCGCGC CGCTGGTGAT CCGCACGATG GTGGGGACCG CCGCCGGGGC GCAGCACTCC CAGGCTCTCG AGGCATGGGC CATGCACACC CCGGGACTCA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGAGGAC GACCCGTGCC TGTTCATCGA ATCGATGAAG CTGTACTACG GCGGCGGCAA GGGGCCGGTT CCGGTAGCCG ACTACGTGAT CCCGCTCGGC CAGGCCGATG TCAAGCGGGT CGGCAGCGAC GTCACCATCT GCACCTACGG CGTGTTGGTG CATGCCGCGC TGGAAGCAGC GAAAACCCTT GCGGGCGAGG GTGTCTCTGT CGAGGTAGTC GATCTGAGGA CGTTGGTTCC GCTGGACCTG CAGACCGTGC TCGACTCGGT GCGCAAGACC AAGCGCCTGG TCGTCGCACA CGAATCGGTC GGCTTCTGCG GTCCCGGTGC CGAGATCGCC GCAGCCGTCG GTACCGAACT GTTCGCAGAG CTTGCGGCGC CGATTCAACG CGTCGCGGGC ACCTACACCC CGGTGCCGAG GGCCGCCACG CTGGAGGCGG CCTGCCGCCC AGATGCCACA AAGCTCAGTG ATGCCGTCAG GAGGATCATG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA