MyabA.00441.a

Putative pyruvate dehydrogenase E1 component, beta subunit

CENTER ID: MyabA.00441.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00441.a.A1.GE30303 Full length( MyabA.00441.a ) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.933
RefSeq: YP_001701644.1
UniProt: B1MJ34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVTTMAQAL NAALRDALHD DDSVVVFGED VGTLGGVFRV TDGLTETFGA NRCFDTPLAE SGIIGFAVGM AMGGFRPVVE MQFDAFAYPA FEQVVSHVAK LRNRTRGVLS VPMVIRVPYA GGIGGVEHHC DSSEAYYAHT PGLKVVTPAT VADAYGLLRE AIDDPDPVVF LEPKRLYFSR ADVELGRGAK IGEAQVLRAG TDVTLIAYGP SVEPALQAAA AAADEGRDIE VVDLRSIAPF DDQSVTASVR KTGRCVIVQE AQGFGGVGAE IAARIQERCF HHLAAPVLRV SGYDIPYPAP RLERWYLPST DRILDAVDRL QWDDHPDSRW VVSA
NT Sequence
ATGACCGTGA CCACTATGGC GCAAGCTCTC AACGCTGCAT TGCGCGATGC GCTGCACGAC GACGATTCGG TGGTGGTGTT CGGCGAGGAC GTCGGCACGT TGGGGGGTGT CTTCCGGGTC ACCGATGGTC TTACCGAAAC CTTCGGCGCC AACAGGTGTT TCGATACTCC GCTGGCAGAA TCGGGAATCA TCGGGTTCGC GGTGGGTATG GCGATGGGGG GCTTTCGCCC GGTGGTCGAG ATGCAATTCG ACGCCTTCGC CTATCCCGCG TTTGAACAAG TCGTGTCCCA CGTGGCCAAA CTGCGCAATC GCACCCGCGG TGTGCTCTCT GTGCCCATGG TGATCCGGGT GCCCTACGCC GGCGGTATCG GAGGCGTCGA ACACCACTGC GACTCCAGCG AGGCCTACTA TGCGCACACG CCGGGACTGA AGGTGGTCAC ACCGGCAACC GTCGCGGACG CCTACGGGCT CCTCAGGGAA GCTATCGACG ATCCCGATCC CGTCGTTTTC CTTGAGCCCA AAAGGCTGTA CTTCTCCCGC GCCGACGTCG AACTGGGTCG AGGTGCCAAA ATCGGCGAGG CGCAGGTGCT GCGTGCCGGG ACCGATGTCA CGCTGATCGC CTACGGCCCG TCGGTGGAAC CTGCGCTGCA GGCAGCCGCC GCAGCCGCCG ACGAAGGCCG AGATATCGAA GTCGTCGACC TTCGGTCCAT CGCACCCTTT GACGACCAGA GCGTGACGGC TTCCGTCCGC AAGACCGGCC GTTGCGTAAT CGTCCAGGAG GCGCAGGGTT TCGGTGGCGT CGGCGCCGAG ATCGCTGCTC GGATCCAGGA GCGCTGTTTC CATCACCTGG CAGCACCTGT TCTGCGCGTC TCCGGATACG ACATCCCCTA CCCCGCGCCT AGGCTCGAGC GATGGTATCT GCCCAGTACC GACCGCATCC TCGACGCGGT CGATCGCCTG CAATGGGACG ACCACCCCGA TAGCCGTTGG GTGGTCAGCG CA
Details for MyabA.00441.a.A1.GE30303
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 107
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVTTMAQA LNAALRDALH DDDSVVVFGE DVGTLGGVFR VTDGLTETFG ANRCFDTPLA ESGIIGFAVG MAMGGFRPVV EMQFDAFAYP AFEQVVSHVA KLRNRTRGVL SVPMVIRVPY AGGIGGVEHH CDSSEAYYAH TPGLKVVTPA TVADAYGLLR EAIDDPDPVV FLEPKRLYFS RADVELGRGA KIGEAQVLRA GTDVTLIAYG PSVEPALQAA AAAADEGRDI EVVDLRSIAP FDDQSVTASV RKTGRCVIVX EAXGFGGVXA EIAARIQERC FHHLAXPXLR VSGXXIPYPA PRLERWXLPS XXXIXXXXXX XXGXXPRXPX GXXXXXXXXX XXXAXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg tgaccactat ggcgcaagct ctcaacgctg cattgcgcga tgcgctgcac gacgacgatt cggtggtggt gttcggcgag gacgtcggca cgttgggggg tgtcttccgg gtcaccgatg gtcttaccga aaccttcggc gccaacaggt gtttcgatac tccgctggca gaatcgggaa tcatcgggtt cgcggtgggt atggcgatgg ggggctttcg cccggtggtc gagatgcaat tcgacgcctt cgcctatccc gcgtttgaac aagtcgtgtc ccacgtggcc aaactgcgca atcgcacccg cggtgtgctc tctgtgccca tggtgatccg ggtgccctac gccggcggta tcggaggcgt cgaacaccac tgcgactcca gcgaggccta ctatgcgcac acgccgggac tgaaggtggt cacaccggca accgtcgcgg acgcctacgg gctcctcagg gaagctatcg acgatcccga tcccgtcgtt ttccttgagc ccaaaaggct gtacttctcc cgcgccgacg tcgaactggg tcgaggtgcc aaaatcggcg aggcgcaggt gctgcgtgcc gggaccgatg tcacgctgat cgcctacggc ccgtcggtgg aacctgcgct gcaggcagcc gccgcagccg ccgacgaagg ccgagatatc gaagtcgtcg accttcggtc catcgcaccc tttgacgacc agagcgtgac ggcttccgtc cgcaagaccg gccgttgcgt aatcgtccan gaggcgcnng gtttcggtgg cgtcngcgcc gagatcgctg ctcggatcca ggagcgctgt ttccatcacc tggcnncacc tgntctgcgc gtctccggat ncnacatccc ctaccccgcg cctaggctcg agcgatggna tctgcccagt ancnacngca tcnnnanncn gnncnatcnn nnnnngggnc nacnnccncg atnnccgnnn ggnngncann ncannnnaan cancannnaa cnannnnnng cnnnnagntn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnntcn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVTTMAQA LNAALRDALH DDDSVVVFGE DVGTLGGVFR VTDGLTETFG ANRCFDTPLA ESGIIGFAVG MAMGGFRPVV EMQFDAFAYP AFEQVVSHVA KLRNRTRGVL SVPMVIRVPY AGGIGGVEHH CDSSEAYYAH TPGLKVVTPA TVADAYGLLR EAIDDPDPVV FLEPKRLYFS RADVELGRGA KIGEAQVLRA GTDVTLIAYG PSVEPALQAA AAAADEGRDI EVVDLRSIAP FDDQSVTASV RKTGRCVIVQ EAQGFGGVGA EIAARIQERC FHHLAAPVLR VSGYDIPYPA PRLERWYLPS TDRILDAVDR LQWDDHPDSR WVVSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgtgacc actatggcgc aagctctcaa cgctgcattg cgcgatgcgc tgcacgacga cgattcggtg gtggtgttcg gcgaggacgt cggcacgttg gggggtgtct tccgggtcac cgatggtctt accgaaacct tcggcgccaa caggtgtttc gatactccgc tggcagaatc gggaatcatc gggttcgcgg tgggtatggc gatggggggc tttcgcccgg tggtcgagat gcaattcgac gccttcgcct atcccgcgtt tgaacaagtc gtgtcccacg tggccaaact gcgcaatcgc acccgcggtg tgctctctgt gcccatggtg atccgggtgc cctacgccgg cggtatcgga ggcgtcgaac accactgcga ctccagcgag gcctactatg cgcacacgcc gggactgaag gtggtcacac cggcaaccgt cgcggacgcc tacgggctcc tcagggaagc tatcgacgat cccgatcccg tcgttttcct tgagcccaaa aggctgtact tctcccgcgc cgacgtcgaa ctgggtcgag gtgccaaaat cggcgaggcg caggtgctgc gtgccgggac cgatgtcacg ctgatcgcct acggcccgtc ggtggaacct gcgctgcagg cagccgccgc agccgccgac gaaggccgag atatcgaagt cgtcgacctt cggtccatcg caccctttga cgaccagagc gtgacggctt ccgtccgcaa gaccggccgt tgcgtaatcg tccaggaggc gcagggtttc ggtggcgtcg gcgccgagat cgctgctcgg atccaggagc gctgtttcca tcacctggca gcacctgttc tgcgcgtctc cggatacgac atcccctacc ccgcgcctag gctcgagcga tggtatctgc ccagtaccga ccgcatcctc gacgcggtcg atcgcctgca atgggacgac caccccgata gccgttgggt ggtcagcgca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa