MyabA.00441.b

Pyruvate dehydrogenase E1 component beta subunit

CENTER ID: MyabA.00441.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00441.b.A1.GE30304 Full length( MyabA.00441.b ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4999
RefSeq: YP_001705639.1
UniProt: B1MMK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRMTMSGAL DAGLRSALED DDKVIVMGED VGKLGGVFRV TDGLQKDFGD HRVIDTPLAE SGIIGTAVGL AMRGYRPVCE IQFDGFVYPA FDQIVSQVAK LHYRTKGAVG MPLTIRIPFG GGIGAVEHHS ESPEAYFAHT AGLRVVSCSS PQDAYDMIRQ AVACDDPVVF FEPKRRYWEK GEVDTALVPV PLGAARVVRS GTAATIAAYG PMVAVANAAA ELAATEGISV EVVDLRSLSP VDFDTLEASV RKTGRLIVVH EAPVFMGLGA EIAARITERC FYHLEAPVLR VGGFALPYPA NKVEHHYLPD AERVMDAVDR AIAA
NT Sequence
ATGACCAGGA TGACTATGTC CGGTGCCCTG GACGCTGGCT TGCGGTCGGC GCTGGAGGAC GACGACAAGG TGATCGTCAT GGGAGAGGAT GTCGGCAAGC TCGGCGGTGT CTTCCGGGTG ACCGACGGGC TGCAGAAGGA TTTCGGCGAC CATCGCGTCA TCGACACGCC GCTGGCCGAG TCCGGAATTA TCGGCACCGC GGTCGGTTTG GCCATGCGGG GATACCGTCC GGTCTGTGAA ATCCAGTTCG ACGGATTCGT ATACCCGGCC TTCGATCAGA TTGTCAGTCA GGTGGCCAAG TTGCACTACC GCACCAAGGG CGCGGTGGGA ATGCCGCTGA CCATCCGAAT TCCCTTCGGG GGCGGAATCG GTGCGGTGGA ACATCATTCG GAGTCACCCG AAGCGTATTT CGCGCATACG GCCGGCCTAC GCGTGGTGTC TTGCAGCTCA CCGCAGGACG CTTACGACAT GATCCGGCAG GCTGTCGCGT GCGACGACCC GGTGGTCTTC TTCGAACCCA AGCGGCGATA CTGGGAGAAG GGCGAGGTCG ATACCGCGCT GGTGCCTGTC CCACTCGGTG CCGCGCGCGT TGTCCGGTCC GGAACCGCGG CCACCATCGC CGCCTATGGA CCGATGGTGG CAGTGGCCAA TGCCGCCGCG GAACTGGCTG CCACGGAGGG CATATCGGTC GAGGTGGTGG ATTTGCGATC GCTTTCTCCG GTTGATTTCG ACACCCTGGA GGCGTCGGTG CGCAAGACCG GACGGCTGAT CGTGGTGCAC GAGGCCCCGG TATTCATGGG TTTGGGCGCC GAGATTGCCG CGCGCATCAC GGAGCGATGC TTCTACCACC TGGAGGCTCC GGTGTTGCGG GTCGGCGGCT TCGCGCTTCC GTACCCGGCC AACAAGGTGG AACATCACTA CCTACCCGAT GCGGAGCGGG TGATGGACGC CGTCGACCGC GCTATCGCCG CC
Details for MyabA.00441.b.A1.GE30304
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRMTMSGA LDAGLRSALE DDDKVIVMGE DVGKLGGVFR VTDGLQKDFG DHRVIDTPLA ESGIIGTAVG LAMRGYRPVC EIQFDGFVYP AFDQIVSQVA KLHYRTKGAV GMPLTIRIPF GGGIGAVEHH SESPEAYFAH TAGLRVVSCS SPQDAYDMIR QAVACDDPVV FFEPKRRYWE KGEVDTALVP VPLGAARVVR SGTAATIAAY GPMVAVANAA AELAATEGIS VEVVDLRSLS PVDFDTLEAS VRKTGRLIVV HEAPVFMGLG AEIAARITER CFYHLEAXVL RVGGFXLXYP ANXVXXHYXX DAXRXXDAVX XLXXXXQXXX XXXXXXXXXX XXXLXAXXXX XXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca ggatgactat gtccggtgcc ctggacgctg gcttgcggtc ggcgctggag gacgacgaca aggtgatcgt catgggagag gatgtcggca agctcggcgg tgtcttccgg gtgaccgacg ggctgcagaa ggatttcggc gaccatcgcg tcatcgacac gccgctggcc gagtccggaa ttatcggcac cgcggtcggt ttggccatgc ggggataccg tccggtctgt gaaatccagt tcgacggatt cgtatacccg gccttcgatc agattgtcag tcaggtggcc aagttgcact accgcaccaa gggcgcggtg ggaatgccgc tgaccatccg aattcccttc gggggcggaa tcggtgcggt ggaacatcat tcggagtcac ccgaagcgta tttcgcgcat acggccggcc tacgcgtggt gtcttgcagc tcaccgcagg acgcttacga catgatccgg caggctgtcg cgtgcgacga cccggtggtc ttcttcgaac ccaagcggcg atactgggag aagggcgagg tcgataccgc gctggtgcct gtcccactcg gtgccgcgcg cgttgtccgg tccggaaccg cggccaccat cgccgcctat ggaccgatgg tggcagtggc caatgccgcc gcggaactgg ctgccacgga gggcatatcg gtcgaggtgg tggatttgcg atcgctttct ccggttgatt tcgacaccct ggaggcgtcn gtgcgcaaga ccggacggct gatcgtggtg cacgaggccc cggtattcat gggtttgggc gccgagattg ccgcgcgcat cacggagcga tgcttctacc acctggaggc tcnngtgttg cgggtcggcg gcttcncgct tncgtacccn gccaacangg tggnanatca ctacntacnn gatgcnnnnc gggngntgga cgcngtcnnc nngctannnc nnnannanca gnacnncanc nnctnnnnnn nnnnnnncnn ctannnaann ngaannganc tnnnngctnn nnnnnnnnnn nnnnnnnnnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRMTMSGA LDAGLRSALE DDDKVIVMGE DVGKLGGVFR VTDGLQKDFG DHRVIDTPLA ESGIIGTAVG LAMRGYRPVC EIQFDGFVYP AFDQIVSQVA KLHYRTKGAV GMPLTIRIPF GGGIGAVEHH SESPEAYFAH TAGLRVVSCS SPQDAYDMIR QAVACDDPVV FFEPKRRYWE KGEVDTALVP VPLGAARVVR SGTAATIAAY GPMVAVANAA AELAATEGIS VEVVDLRSLS PVDFDTLEAS VRKTGRLIVV HEAPVFMGLG AEIAARITER CFYHLEAPVL RVGGFALPYP ANKVEHHYLP DAERVMDAVD RAIAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaggatg actatgtccg gtgccctgga cgctggcttg cggtcggcgc tggaggacga cgacaaggtg atcgtcatgg gagaggatgt cggcaagctc ggcggtgtct tccgggtgac cgacgggctg cagaaggatt tcggcgacca tcgcgtcatc gacacgccgc tggccgagtc cggaattatc ggcaccgcgg tcggtttggc catgcgggga taccgtccgg tctgtgaaat ccagttcgac ggattcgtat acccggcctt cgatcagatt gtcagtcagg tggccaagtt gcactaccgc accaagggcg cggtgggaat gccgctgacc atccgaattc ccttcggggg cggaatcggt gcggtggaac atcattcgga gtcacccgaa gcgtatttcg cgcatacggc cggcctacgc gtggtgtctt gcagctcacc gcaggacgct tacgacatga tccggcaggc tgtcgcgtgc gacgacccgg tggtcttctt cgaacccaag cggcgatact gggagaaggg cgaggtcgat accgcgctgg tgcctgtccc actcggtgcc gcgcgcgttg tccggtccgg aaccgcggcc accatcgccg cctatggacc gatggtggca gtggccaatg ccgccgcgga actggctgcc acggagggca tatcggtcga ggtggtggat ttgcgatcgc tttctccggt tgatttcgac accctggagg cgtcggtgcg caagaccgga cggctgatcg tggtgcacga ggccccggta ttcatgggtt tgggcgccga gattgccgcg cgcatcacgg agcgatgctt ctaccacctg gaggctccgg tgttgcgggt cggcggcttc gcgcttccgt acccggccaa caaggtggaa catcactacc tacccgatgc ggagcgggtg atggacgccg tcgaccgcgc tatcgccgcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa