MymaA.00441.a

Pyruvate dehydrogenase E1 component (Beta subunit) PdhB

CENTER ID: MymaA.00441.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00441.a.A1.GE30026 Full length( MymaA.00441.a ) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4119
RefSeq: YP_001852109.1
UniProt: B2HNK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQIADPPAR PGPAPAAPVV GTMQSLTMVQ ALNQALHDAM AADDRVLVFG EDVGIAGGVF RVTEGLAETF GEHRCFDTPL AESALIGIAV GLALRGFVPV PEIQFDGFSY PAFDQVVSHL AKYRTRTRGE VNMAVTVRIP SFGGIGAAEH HSDSTESYWA HTAGLKVVVP STPGDAYWLL RHAIACPDPV MYLEPKRRYQ VRGPVDTSRP EPAIGQAMIR RAGADVTVIT YGNLVSTALS AAEDAAHQQG WSLEVIDLRS LIPLDFETIA ASIRRTGRCV VLHEGPRSLG YGAGLAARIQ EELFYELEAP VLRACGFDTP YPPARLERLW LPGPDRLLDC VERVLGQP
NT Sequence
ATGACACAGA TCGCCGATCC GCCAGCCCGT CCCGGCCCGG CTCCGGCCGC GCCGGTGGTG GGCACCATGC AGTCATTGAC CATGGTGCAA GCGCTCAACC AGGCCTTGCA TGACGCGATG GCGGCTGACG ATCGCGTGCT GGTCTTCGGC GAGGATGTTG GGATTGCGGG CGGCGTCTTC CGGGTGACTG AGGGATTGGC CGAAACCTTT GGTGAGCACA GATGCTTTGA CACCCCGTTA GCCGAGTCGG CTTTGATCGG CATCGCGGTG GGGCTGGCGC TGCGCGGCTT CGTGCCGGTG CCCGAGATCC AGTTCGACGG CTTTTCCTAC CCGGCATTCG ATCAAGTGGT CAGTCACCTG GCCAAGTACC GGACCCGCAC CCGCGGCGAG GTCAATATGG CGGTAACCGT TCGGATCCCG TCGTTCGGCG GCATCGGCGC CGCGGAACAT CATTCGGACT CCACCGAGTC CTACTGGGCG CACACGGCGG GCTTGAAGGT GGTGGTTCCC TCAACCCCCG GCGATGCCTA CTGGCTGTTG CGCCACGCCA TCGCCTGCCC AGATCCCGTC ATGTATCTGG AGCCCAAGCG CCGGTACCAG GTTCGCGGAC CCGTCGATAC CAGCCGGCCG GAACCTGCGA TCGGTCAAGC GATGATTCGC CGGGCCGGGG CCGACGTGAC CGTCATCACC TACGGAAACC TGGTCTCGAC CGCACTGTCG GCCGCTGAAG ACGCTGCACA CCAACAGGGT TGGAGTCTAG AAGTCATCGA CCTCAGGTCG CTGATCCCCT TGGACTTCGA AACCATCGCC GCCTCGATCC GCCGGACCGG ACGGTGTGTG GTGCTGCACG AGGGGCCGCG CAGCCTGGGC TACGGCGCCG GGCTGGCCGC CCGGATACAG GAGGAGTTGT TCTACGAACT GGAGGCTCCG GTATTGCGCG CCTGCGGATT TGACACTCCG TATCCGCCGG CACGATTGGA GCGGCTGTGG CTGCCCGGGC CCGACCGGCT GCTGGATTGT GTCGAACGGG TGCTGGGGCA GCCA
Details for MymaA.00441.a.A1.GE30026
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQIADPPA RPGPAPAAPV VGTMQSLTMV QALNQALHDA MAADDRVLVF GEDVGIAGGV FRVTEGLAET FGEHRCFDTP LAESALIGIA VGLALRGFVP VPEIQFDGFS YPAFDQVVSH LAKYRTRTRG EVNMAVTVRI PSFGGIGAAE HHSDSTESYW AHTAGLKVVV PSTPGDAYWL LRHAIACPDP VMYLEPKRRY QVRGPVDTSR PEPAIGQAMI RRAGADVTVI TYGNLVSTAL SAAEDAAHQQ GWSLEVIDLR SLIPLDFETI AASIRRTGRC VVLHEGPRXX GXXAGXPXYR XSCXTNXXXV XXXLTXRXXX DXXXXXXXRX XXXXGXGXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaca cagatcgccg atccgccagc ccgtcccggc ccggctccgg ccgcgccggt ggtgggcacc atgcagtcat tgaccatggt gcaagcgctc aaccaggcct tgcatgacgc gatggcggct gacgatcgcg tgctggtctt cggcgaggat gttgggattg cgggcggcgt cttccgggtg actgagggat tggccgaaac ctttggtgag cacagatgct ttgacacccc gttagccgag tcggctttga tcggcatcgc ggtggggctg gcgctgcgcg gcttcgtgcc ggtgcccgag atccagttcg acggcttttc ctacccggca ttcgatcaag tggtcagtca cctggccaag taccggaccc gcacccgcgg cgaggtcaat atggcggtaa ccgttcggat cccgtcgttc ggcggcatcg gcgccgcgga acatcattcg gactccaccg agtcctactg ggcgcacacg gcgggcttga aggtggtggt tccctcaacc cccggcgatg cctactggct gttgcgccac gccatcgcct gcccagatcc cgtcatgtat ctggagccca agcgccggta ccaggttcgc ggacccgtcg ataccagccg gccggaacct gcgatcggtc aagcgatgat tcgccgggcc ggggccgacg tgaccgtcat cacctacgga aacctggtct cgaccgcact gtcggccgct gaagacgctg cacaccaaca gggttggagt ctagaagtca tcgacctcag gtcgctgatc cccttggact tcgaaaccat cgccgcctcn atccgccgga ccggacggtg tgtggtgctg cacgaggggc cgcgcannnt gggctangnc gccgggntgc ccngntacag gagnagttgt nctacgaact nnngctnngt ntgngnnnnn ttgacnnnnc gtancnngnn cgatnnnngn nnngnnnnnn nccnacgnnn ctgntnnntc nanggnnctg ggnnnnnnna aannnnnnnn nnannnngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQIADPPA RPGPAPAAPV VGTMQSLTMV QALNQALHDA MAADDRVLVF GEDVGIAGGV FRVTEGLAET FGEHRCFDTP LAESALIGIA VGLALRGFVP VPEIQFDGFS YPAFDQVVSH LAKYRTRTRG EVNMAVTVRI PSFGGIGAAE HHSDSTESYW AHTAGLKVVV PSTPGDAYWL LRHAIACPDP VMYLEPKRRY QVRGPVDTSR PEPAIGQAMI RRAGADVTVI TYGNLVSTAL SAAEDAAHQQ GWSLEVIDLR SLIPLDFETI AASIRRTGRC VVLHEGPRSL GYGAGLAARI QEELFYELEA PVLRACGFDT PYPPARLERL WLPGPDRLLD CVERVLGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacagatc gccgatccgc cagcccgtcc cggcccggct ccggccgcgc cggtggtggg caccatgcag tcattgacca tggtgcaagc gctcaaccag gccttgcatg acgcgatggc ggctgacgat cgcgtgctgg tcttcggcga ggatgttggg attgcgggcg gcgtcttccg ggtgactgag ggattggccg aaacctttgg tgagcacaga tgctttgaca ccccgttagc cgagtcggct ttgatcggca tcgcggtggg gctggcgctg cgcggcttcg tgccggtgcc cgagatccag ttcgacggct tttcctaccc ggcattcgat caagtggtca gtcacctggc caagtaccgg acccgcaccc gcggcgaggt caatatggcg gtaaccgttc ggatcccgtc gttcggcggc atcggcgccg cggaacatca ttcggactcc accgagtcct actgggcgca cacggcgggc ttgaaggtgg tggttccctc aacccccggc gatgcctact ggctgttgcg ccacgccatc gcctgcccag atcccgtcat gtatctggag cccaagcgcc ggtaccaggt tcgcggaccc gtcgatacca gccggccgga acctgcgatc ggtcaagcga tgattcgccg ggccggggcc gacgtgaccg tcatcaccta cggaaacctg gtctcgaccg cactgtcggc cgctgaagac gctgcacacc aacagggttg gagtctagaa gtcatcgacc tcaggtcgct gatccccttg gacttcgaaa ccatcgccgc ctcgatccgc cggaccggac ggtgtgtggt gctgcacgag gggccgcgca gcctgggcta cggcgccggg ctggccgccc ggatacagga ggagttgttc tacgaactgg aggctccggt attgcgcgcc tgcggatttg acactccgta tccgccggca cgattggagc ggctgtggct gcccgggccc gaccggctgc tggattgtgt cgaacgggtg ctggggcaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a