MytuD.00185.a

PROBABLE ACYL-CoA DEHYDROGENASE FADE25

CENTER ID: MytuD.00185.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00185.a.A1.PS00425 Full length( MytuD.00185.a ) 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3661
RefSeq: NP_217791.1
TubercuList: Rv3274c
MTB Network Portal: Rv3274c
UniProt: P9WQG1

Orthologues for Rv3274c

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 1 1 0
smegmatis 90 100 1 1 0
ulcerans 90 100 0 0 0
thermoresistibile 90 86 1 1 1
avium 89 100 0 0 0
abscessus 89 99 1 1 0
smegmatis 45 95 1 1 0
thermoresistibile 31 72 1 1 1
smegmatis 26 87 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVGWAGNPSF DLFKLPEEHD EMRSAIRALA EKEIAPHAAE VDEKARFPEE ALVALNSSGF NAVHIPEEYG GQGADSVATC IVIEEVARVD ASASLIPAVN KLGTMGLILR GSEELKKQVL PALAAEGAMA SYALSEREAG SDAASMRTRA KADGDHWILN GAKCWITNGG KSTWYTVMAV TDPDRGANGI SAFMVHKDDE GFTVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKDRKQF GESISTFQAV QFMLADMAMK VEAARLMVYS AAARAERGEP DLGFISAASK CFASDVAMEV TTDAVQLFGG AGYTTDFPVE RFMRDAKITQ IYEGTNQIQR VVMSRALLR
NT Sequence
ATGGTTGGAT GGGCCGGAAA CCCGTCGTTC GATCTGTTCA AGCTGCCCGA GGAACACGAC GAAATGCGAT CGGCGATACG GGCTTTGGCG GAAAAGGAGA TCGCTCCGCA TGCCGCCGAG GTGGACGAGA AGGCGCGGTT CCCCGAAGAA GCGCTAGTGG CGCTCAATTC CTCCGGTTTC AACGCCGTCC ACATTCCGGA GGAGTACGGC GGTCAGGGTG CCGACTCGGT AGCTACCTGC ATCGTGATCG AAGAGGTGGC CCGCGTCGAC GCGTCCGCGT CGTTGATCCC CGCGGTCAAC AAGCTGGGCA CCATGGGCCT GATCCTGCGG GGCTCCGAGG AGCTGAAGAA GCAGGTGCTG CCGGCGTTGG CCGCCGAAGG GGCGATGGCT TCCTATGCAT TGAGTGAACG CGAGGCCGGC AGTGACGCGG CGTCCATGCG GACGCGGGCC AAGGCCGATG GGGATCACTG GATTCTCAAC GGCGCCAAGT GCTGGATCAC CAACGGCGGC AAGTCGACCT GGTACACGGT GATGGCGGTG ACCGATCCCG ACCGGGGCGC CAACGGCATC TCGGCGTTCA TGGTGCACAA GGACGACGAG GGGTTCACCG TCGGTCCGAA AGAACGCAAG CTCGGGATCA AGGGGTCACC GACCACCGAG CTGTACTTCG AGAACTGCCG CATCCCCGGC GATCGCATCA TCGGTGAGCC CGGTACCGGT TTCAAGACCG CGCTGGCCAC GTTGGACCAC ACCCGTCCCA CGATTGGCGC GCAGGCCGTG GGTATCGCCC AGGGCGCGCT GGATGCTGCC ATCGCCTACA CCAAGGACCG CAAGCAGTTC GGTGAGTCGA TCAGTACGTT TCAGGCCGTG CAGTTCATGC TGGCCGACAT GGCGATGAAG GTGGAGGCGG CTCGGCTGAT GGTTTACTCC GCCGCCGCCC GCGCCGAACG CGGTGAGCCG GATCTGGGCT TCATTTCGGC GGCCTCGAAG TGCTTCGCCT CCGACGTCGC GATGGAGGTC ACCACCGATG CCGTACAACT GTTCGGCGGC GCCGGCTACA CCACCGACTT CCCGGTCGAG CGGTTTATGC GCGACGCCAA GATCACCCAG ATCTATGAGG GCACCAATCA GATTCAGCGG GTAGTGATGT CGCGGGCGCT ACTGCGC
Details for MytuD.00185.a.A1.PS00425
PURIFICATION DATe: 12/4/2009
CONCENTRATION: 3.46mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: data unavailable
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 38
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGWAGNPS FDLFKLPEEH DEMRSAIRAL AEKEIAPHAA EVDEKARFPE EALVALNSSG FNAVHIPEEY GGQGADSVAT CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSEELKKQV LPALAAEGGD GFLCIE
Validated NT Sequence
ttattagcgc agtagcgccc gcgacatcac tacccgctga atctgattgg tgccctcata gatctgggtg atcttggcgt cgcgcataaa ccgctcgacc gggaagtcgg tggtgtagcc ggcgccgccg aacagttgta cggcatcggt ggtgacctcc atcgcgacgt cggaggcgaa gcacttcgag gccgccgaaa tgaagcccag atccggctca ccgcgttcgg cgcgggcggc ggcggagtaa accatcagcc gagccgcctc caccttcatc gccatgtcgg ccagcatgaa ctgcacggcc tgaaacgtac tgatcgactc accgaactgc ttgcggtcct tggtgtaggc gatggcagca tccagcgcgc cctgggcgat acccacggcc tgcgcgccaa tcgtgggacg ggtgtggtcc aacgtggcca gcgcggtctt gaaaccggta ccgggctcac cgatgatgcg atcgccgggg atgcggcagt tctcgaagta cagctcggtg gtcggtgacc ccttgatccc gagcttgcgt tctttcggac cgacggtgaa cccctcgtcg tccttgtgca ccatgaacgc cgagatgccg ttggcgcccc ggtcgggatc ggtcaccgcc atcaccgtgt accaggtcga cttgccgccg ttggtgatcc agcacttggc gccgttgaga atccagtgat ccccatcggc cttggcccgc gtccgcatgg acgccgcgtc actgccggcc tcgcgttcac tcaatgcata ggaagccatc gcccccttcg gcggccaacg ccggcagcac ctgcttcttc agctcctcgg agccccgcag gatcaggccc atggtgccca gcttgttgac cgcggggatc aacgacgcgg acgcgtcgac gcgggccacc tcttcgatca cgatgcaggt agctaccgag tcggcaccct gaccgccgta ctcctccgga atgtggacgg cgttgaaacc ggaggaattg agcgccacta gcgcttcttc ggggaaccgc gccttctcgt ccacctcggc ggcatgcgga gcgatctcct tttccgccaa agcccgtatc gccgatcgca tttcgtcgtg ttcctcgggc agcttgaaca gatcgaacga cgggtttccg gcccatccaa ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGWAGNPS FDLFKLPEEH DEMRSAIRAL AEKEIAPHAA EVDEKARFPE EALVALNSSG FNAVHIPEEY GGQGADSVAT CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSEELKKQV LPALAAEGAM ASYALSEREA GSDAASMRTR AKADGDHWIL NGAKCWITNG GKSTWYTVMA VTDPDRGANG ISAFMVHKDD EGFTVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKDRKQ FGESISTFQA VQFMLADMAM KVEAARLMVY SAAARAERGE PDLGFISAAS KCFASDVAME VTTDAVQLFG GAGYTTDFPV ERFMRDAKIT QIYEGTNQIQ RVVMSRALLR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGGATGG GCCGGAAACC CGTCGTTCGA TCTGTTCAAG CTGCCCGAGG AACACGACGA AATGCGATCG GCGATACGGG CTTTGGCGGA AAAGGAGATC GCTCCGCATG CCGCCGAGGT GGACGAGAAG GCGCGGTTCC CCGAAGAAGC GCTAGTGGCG CTCAATTCCT CCGGTTTCAA CGCCGTCCAC ATTCCGGAGG AGTACGGCGG TCAGGGTGCC GACTCGGTAG CTACCTGCAT CGTGATCGAA GAGGTGGCCC GCGTCGACGC GTCCGCGTCG TTGATCCCCG CGGTCAACAA GCTGGGCACC ATGGGCCTGA TCCTGCGGGG CTCCGAGGAG CTGAAGAAGC AGGTGCTGCC GGCGTTGGCC GCCGAAGGGG CGATGGCTTC CTATGCATTG AGTGAACGCG AGGCCGGCAG TGACGCGGCG TCCATGCGGA CGCGGGCCAA GGCCGATGGG GATCACTGGA TTCTCAACGG CGCCAAGTGC TGGATCACCA ACGGCGGCAA GTCGACCTGG TACACGGTGA TGGCGGTGAC CGATCCCGAC CGGGGCGCCA ACGGCATCTC GGCGTTCATG GTGCACAAGG ACGACGAGGG GTTCACCGTC GGTCCGAAAG AACGCAAGCT CGGGATCAAG GGGTCACCGA CCACCGAGCT GTACTTCGAG AACTGCCGCA TCCCCGGCGA TCGCATCATC GGTGAGCCCG GTACCGGTTT CAAGACCGCG CTGGCCACGT TGGACCACAC CCGTCCCACG ATTGGCGCGC AGGCCGTGGG TATCGCCCAG GGCGCGCTGG ATGCTGCCAT CGCCTACACC AAGGACCGCA AGCAGTTCGG TGAGTCGATC AGTACGTTTC AGGCCGTGCA GTTCATGCTG GCCGACATGG CGATGAAGGT GGAGGCGGCT CGGCTGATGG TTTACTCCGC CGCCGCCCGC GCCGAACGCG GTGAGCCGGA TCTGGGCTTC ATTTCGGCGG CCTCGAAGTG CTTCGCCTCC GACGTCGCGA TGGAGGTCAC CACCGATGCC GTACAACTGT TCGGCGGCGC CGGCTACACC ACCGACTTCC CGGTCGAGCG GTTTATGCGC GACGCCAAGA TCACCCAGAT CTATGAGGGC ACCAATCAGA TTCAGCGGGT AGTGATGTCG CGGGCGCTAC TGCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA