MythA.00185.a
Acyl-CoA dehydrogenase (A0QTF4 homolog)
| CENTER ID: | MythA.00185.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00185.a.A1.GE28712 | Full length( MythA.00185.a ) | 1 | 422 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00185.a.A1.PS00589 | Full length( MythA.00185.a ) | 1 | 422 |
Structures
3NF4
DEPOSITED: 6/9/2010
DETERMINATION: XRay
CLONE: MythA.00185.a.A1.GE28712
PROTEIN: MythA.00185.a.A1.PS00589
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|1078020.3.peg.1081 |
| RefSeq: | WP_003924563.1 |
| UniProt: | G7CDN2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHPMAVDRLL PSQEAAELIE LTREIADKVL DPIVDRHEKD ETYPEGVFEQ LGAAGLLSLP QPEEWGGGGQ PYEVYLQVLE EIAARWASVA VAXXXXXXXX XXXXXXXXXX XXXXXXXXXX XWLPGMLSGE QIGAYSLSEP QAGSDAAALR WAAXXXXXXX XXXXXXXXXX XXXXKADFYT LFARTGEGSR GVSCFLVPAD QPGLSFGKPE EKMGLHAVPT TSAFYDNARI DADRRIGEEG QGLQIAFSAL DSGRLGIAAV ATGLAQAALD EAVAYANERT AFGRKIIDHQ GLGFLLADXX XXXXXXXXXX XXXXXXXXXX PGPAVLATGQ HREADRDRCR DEGHHRCGAG VRRGRLHPRL PGGALHAGSQ DHADLRGHEP DSAAGHRPRP HPITARKPVF RTGRSRRRCS TPSAPANAVP AG
NT Sequence
ATTCACCCCA TGGCCGTGGA CCGTCTGCTG CCCTCACAGG AGGCGGCTGA GCTTATCGAA CTCACCCGGG AGATCGCCGA CAAGGTCCTC GACCCGATCG TGGATCGGCA CGAGAAGGAC GAGACCTATC CGGAGGGGGT GTTCGAGCAA CTCGGCGCCG CCGGTCTGCT GAGTTTGCCA CAGCCCGAGG AATGGGGTGG CGGGGGTCAG CCGTACGAGG TCTACCTGCA GGTTCTGGAG GAGATCGCGG CTCGGTGGGC GTCCGTCGCG GTCGCCCNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGGCTGC CGGGCATGCT GTCCGGTGAG CAGATCGGCG CCTACAGCCT GTCCGAGCCG CAGGCCGGGT CGGACGCGGC GGCGTTGCGG TGGGCGGCGN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCAAGGCCGA CTTCTATACC CTCTTCGCCC GCACCGGAGA GGGCTCGCGC GGGGTGTCCT GTTTCCTGGT GCCCGCCGAT CAGCCCGGGC TGAGTTTCGG CAAACCCGAG GAGAAGATGG GTCTGCACGC GGTGCCCACC ACCTCGGCCT TCTACGACAA CGCCCGCATC GACGCCGACC GGCGCATCGG TGAGGAGGGG CAAGGTCTGC AGATCGCGTT CAGCGCACTG GATTCCGGCC GGCTCGGCAT CGCCGCGGTG GCCACCGGGT TGGCCCAGGC CGCGCTGGAT GAGGCGGTCG CGTACGCCAA CGAACGAACC GCCTTCGGCC GCAAGATCAT CGACCACCAA GGACTGGGTT TCCTGTTGGC CGACATNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA CCAGGGCCGG CCGTACTCGC AACAGGCCAG CATCGCGAAG CTGACCGCGA CCGATGCCGC GATGAAGGTC ACCACCGATG CGGTGCAGGT GTTCGGCGGG GTCGGCTACA CCCGCGACTA CCGGGTGGAG CGCTACATGC GGGAAGCCAA GATCATGCAG ATCTTCGAGG GCACGAACCA GATTCAGCGG CTGGTCATCG CCCGCGGCCT CACCCGATAA CCGCTCGGAA ACCGGTTTTC AGAACCGGGA GATCACGTCG GAGGTGTAGT ACTCCATCGG CACCAGCGAA TGCGGTGCCG GCCGGC
Details for MythA.00185.a.A1.GE28712
| HARVESTED ON: | 3/1/2010 |
| SEQUENCED ON: | 3/5/2010 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 68 |
| PERCENT COVERAGE: | 86 |
Validated AA Sequence
MIHPMAVDRL LPSQEAAELI ELTREIADKV LDPIVDRHEK DETYPEGVFE QLGAAGLLSL PQPEEWGGGG QPYEVYLQVL EEIAARWASV AVAVSVHSLS SHPLLVFGTE EQKKRWLPGM LSGEQIGAYS LSEPQAGSDA AALRCAATPT DGGYVINGSK SWITHGGKAD FYTLFARTGE GSRGVSCFLV PADQPGLSFG KPEEKMGLHA VPTTSAFYDN ARIDADRRIG EEGQGLQIAF SALDSGRLGI AAVATGLAQA ALDEAVAYAN ERTAFGRKII DHQGLGFLLA DMAAAVATAR ATYLDAARRR DQGRPYSQQA SIAKLTATDA AMKVTTDAVQ VFGGVGYTRD YRVERYMREA KIMQIFEGTN QIQRLVIARG LTR
Validated NT Sequence
ttattagccg gccggcaccg cattcgctgg tgccgatgga gtactacacc tccgacgtga tctcccggtt ctgaaaaccg gtttccgagc ggttatcggg tgaggccgcg ggcgatgacc agccgctgaa tctggttcgt gccctcgaag atctgcatga tcttggcttc ccgcatgtag cgctccaccc ggtagtcgcg ggtgtagccg accccgccga acacctgcac cgcatcggtg gtgaccttca tcgcggcatc ggtcgcggtc agcttcgcga tgctggcctg ttgcgagtac ggccggccct ggtcgcggcg gcgggcggcg tccagatagg tggcgcgggc ggtggccacc gcggcggcca tgtcggccaa caggaaaccc agtccttggt ggtcgatgat cttgcggccg aaggcggttc gttcgttggc gtacgcgacc gcctcatcca gcgcggcctg ggccaacccg gtggccaccg cggcgatgcc gagccggccg gaatccagtg cgctgaacgc gatctgcaga ccttgcccct cctcaccgat gcgccggtcg gcgtcgatgc gggcgttgtc gtagaaggcc gaggtggtgg gcaccgcgtg cagacccatc ttctcctcgg gtttgccgaa actcagcccg ggctgatcgg cgggcaccag gaaacaggac accccgcgcg agccctctcc ggtgcgggcg aagagggtat agaagtcggc cttgccgccg tgggtgatcc acgattttga gccattgatc acatagccgc cgtccgtcgg cgtcgccgca caccgcaacg ccgccgcgtc cgacccggcc tgcggctcgg acaggctgta ggcgccgatc tgctcaccgg acagcatgcc cggcagccag cgcttcttct gctcctcggt gccgaacacc agcagcgggt gggacgacag gctgtgcacg ctcacggcga ccgcgacgga cgcccaccga gccgcgatct cctccagaac ctgcaggtag acctcgtacg gctgaccccc gccaccccat tcctcgggct gtggcaaact cagcagaccg gcggcgccga gttgctcgaa caccccctcc ggataggtct cgtccttctc gtgccgatcc acgatcgggt cgaggacctt gtcggcgatc tcccgggtga gttcgataag ctcagccgcc tcctgtgagg gcagcagacg gtccacggcc atggggtgaa tcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIHPMAVDR LLPSQEAAEL IELTREIADK VLDPIVDRHE KDETYPEGVF EQLGAAGLLS LPQPEEWGGG GQPYEVYLQV LEEIAARWAS VAVAXXXXXX XXXXXXXXXX XXXXXXXXXX XXXWLPGMLS GEQIGAYSLS EPQAGSDAAA LRWAAXXXXX XXXXXXXXXX XXXXXXKADF YTLFARTGEG SRGVSCFLVP ADQPGLSFGK PEEKMGLHAV PTTSAFYDNA RIDADRRIGE EGQGLQIAFS ALDSGRLGIA AVATGLAQAA LDEAVAYANE RTAFGRKIID HQGLGFLLAD XXXXXXXXXX XXXXXXXXXX XXPGPAVLAT GQHREADRDR CRDEGHHRCG AGVRRGRLHP RLPGGALHAG SQDHADLRGH EPDSAAGHRP RPHPITARKP VFRTGRSRRR CSTPSAPANA VPAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcacccc atggccgtgg accgtctgct gccctcacag gaggcggctg agcttatcga actcacccgg gagatcgccg acaaggtcct cgacccgatc gtggatcggc acgagaagga cgagacctat ccggaggggg tgttcgagca actcggcgcc gccggtctgc tgagtttgcc acagcccgag gaatggggtg gcgggggtca gccgtacgag gtctacctgc aggttctgga ggagatcgcg gctcggtggg cgtccgtcgc ggtcgcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggctg ccgggcatgc tgtccggtga gcagatcggc gcctacagcc tgtccgagcc gcaggccggg tcggacgcgg cggcgttgcg gtgggcggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaaggccg acttctatac cctcttcgcc cgcaccggag agggctcgcg cggggtgtcc tgtttcctgg tgcccgccga tcagcccggg ctgagtttcg gcaaacccga ggagaagatg ggtctgcacg cggtgcccac cacctcggcc ttctacgaca acgcccgcat cgacgccgac cggcgcatcg gtgaggaggg gcaaggtctg cagatcgcgt tcagcgcact ggattccggc cggctcggca tcgccgcggt ggccaccggg ttggcccagg ccgcgctgga tgaggcggtc gcgtacgcca acgaacgaac cgccttcggc cgcaagatca tcgaccacca aggactgggt ttcctgttgg ccgacatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accagggccg gccgtactcg caacaggcca gcatcgcgaa gctgaccgcg accgatgccg cgatgaaggt caccaccgat gcggtgcagg tgttcggcgg ggtcggctac acccgcgact accgggtgga gcgctacatg cgggaagcca agatcatgca gatcttcgag ggcacgaacc agattcagcg gctggtcatc gcccgcggcc tcacccgata accgctcgga aaccggtttt cagaaccggg agatcacgtc ggaggtgtag tactccatcg gcaccagcga atgcggtgcc ggccggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MythA.00185.a.A1.PS00589
| PURIFICATION DATe: | 4/29/2010 |
| CONCENTRATION: | 69.67mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 68 |
| PERCENT COVERAGE: | 86 |
Protocol Notes
notes unavailable
Validated AA Sequence
MIHPMAVDRL LPSQEAAELI ELTREIADKV LDPIVDRHEK DETYPEGVFE QLGAAGLLSL PQPEEWGGGG QPYEVYLQVL EEIAARWASV AVAVSVHSLS SHPLLVFGTE EQKKRWLPGM LSGEQIGAYS LSEPQAGSDA AALRCAATPT DGGYVINGSK SWITHGGKAD FYTLFARTGE GSRGVSCFLV PADQPGLSFG KPEEKMGLHA VPTTSAFYDN ARIDADRRIG EEGQGLQIAF SALDSGRLGI AAVATGLAQA ALDEAVAYAN ERTAFGRKII DHQGLGFLLA DMAAAVATAR ATYLDAARRR DQGRPYSQQA SIAKLTATDA AMKVTTDAVQ VFGGVGYTRD YRVERYMREA KIMQIFEGTN QIQRLVIARG LTR
Validated NT Sequence
ttattagccg gccggcaccg cattcgctgg tgccgatgga gtactacacc tccgacgtga tctcccggtt ctgaaaaccg gtttccgagc ggttatcggg tgaggccgcg ggcgatgacc agccgctgaa tctggttcgt gccctcgaag atctgcatga tcttggcttc ccgcatgtag cgctccaccc ggtagtcgcg ggtgtagccg accccgccga acacctgcac cgcatcggtg gtgaccttca tcgcggcatc ggtcgcggtc agcttcgcga tgctggcctg ttgcgagtac ggccggccct ggtcgcggcg gcgggcggcg tccagatagg tggcgcgggc ggtggccacc gcggcggcca tgtcggccaa caggaaaccc agtccttggt ggtcgatgat cttgcggccg aaggcggttc gttcgttggc gtacgcgacc gcctcatcca gcgcggcctg ggccaacccg gtggccaccg cggcgatgcc gagccggccg gaatccagtg cgctgaacgc gatctgcaga ccttgcccct cctcaccgat gcgccggtcg gcgtcgatgc gggcgttgtc gtagaaggcc gaggtggtgg gcaccgcgtg cagacccatc ttctcctcgg gtttgccgaa actcagcccg ggctgatcgg cgggcaccag gaaacaggac accccgcgcg agccctctcc ggtgcgggcg aagagggtat agaagtcggc cttgccgccg tgggtgatcc acgattttga gccattgatc acatagccgc cgtccgtcgg cgtcgccgca caccgcaacg ccgccgcgtc cgacccggcc tgcggctcgg acaggctgta ggcgccgatc tgctcaccgg acagcatgcc cggcagccag cgcttcttct gctcctcggt gccgaacacc agcagcgggt gggacgacag gctgtgcacg ctcacggcga ccgcgacgga cgcccaccga gccgcgatct cctccagaac ctgcaggtag acctcgtacg gctgaccccc gccaccccat tcctcgggct gtggcaaact cagcagaccg gcggcgccga gttgctcgaa caccccctcc ggataggtct cgtccttctc gtgccgatcc acgatcgggt cgaggacctt gtcggcgatc tcccgggtga gttcgataag ctcagccgcc tcctgtgagg gcagcagacg gtccacggcc atggggtgaa tcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIHPMAVDR LLPSQEAAEL IELTREIADK VLDPIVDRHE KDETYPEGVF EQLGAAGLLS LPQPEEWGGG GQPYEVYLQV LEEIAARWAS VAVAXXXXXX XXXXXXXXXX XXXXXXXXXX XXXWLPGMLS GEQIGAYSLS EPQAGSDAAA LRWAAXXXXX XXXXXXXXXX XXXXXXKADF YTLFARTGEG SRGVSCFLVP ADQPGLSFGK PEEKMGLHAV PTTSAFYDNA RIDADRRIGE EGQGLQIAFS ALDSGRLGIA AVATGLAQAA LDEAVAYANE RTAFGRKIID HQGLGFLLAD XXXXXXXXXX XXXXXXXXXX XXPGPAVLAT GQHREADRDR CRDEGHHRCG AGVRRGRLHP RLPGGALHAG SQDHADLRGH EPDSAAGHRP RPHPITARKP VFRTGRSRRR CSTPSAPANA VPAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTCACCCC ATGGCCGTGG ACCGTCTGCT GCCCTCACAG GAGGCGGCTG AGCTTATCGA ACTCACCCGG GAGATCGCCG ACAAGGTCCT CGACCCGATC GTGGATCGGC ACGAGAAGGA CGAGACCTAT CCGGAGGGGG TGTTCGAGCA ACTCGGCGCC GCCGGTCTGC TGAGTTTGCC ACAGCCCGAG GAATGGGGTG GCGGGGGTCA GCCGTACGAG GTCTACCTGC AGGTTCTGGA GGAGATCGCG GCTCGGTGGG CGTCCGTCGC GGTCGCCCNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTGGCTG CCGGGCATGC TGTCCGGTGA GCAGATCGGC GCCTACAGCC TGTCCGAGCC GCAGGCCGGG TCGGACGCGG CGGCGTTGCG GTGGGCGGCG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCAAGGCCG ACTTCTATAC CCTCTTCGCC CGCACCGGAG AGGGCTCGCG CGGGGTGTCC TGTTTCCTGG TGCCCGCCGA TCAGCCCGGG CTGAGTTTCG GCAAACCCGA GGAGAAGATG GGTCTGCACG CGGTGCCCAC CACCTCGGCC TTCTACGACA ACGCCCGCAT CGACGCCGAC CGGCGCATCG GTGAGGAGGG GCAAGGTCTG CAGATCGCGT TCAGCGCACT GGATTCCGGC CGGCTCGGCA TCGCCGCGGT GGCCACCGGG TTGGCCCAGG CCGCGCTGGA TGAGGCGGTC GCGTACGCCA ACGAACGAAC CGCCTTCGGC CGCAAGATCA TCGACCACCA AGGACTGGGT TTCCTGTTGG CCGACATNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ACCAGGGCCG GCCGTACTCG CAACAGGCCA GCATCGCGAA GCTGACCGCG ACCGATGCCG CGATGAAGGT CACCACCGAT GCGGTGCAGG TGTTCGGCGG GGTCGGCTAC ACCCGCGACT ACCGGGTGGA GCGCTACATG CGGGAAGCCA AGATCATGCA GATCTTCGAG GGCACGAACC AGATTCAGCG GCTGGTCATC GCCCGCGGCC TCACCCGATA ACCGCTCGGA AACCGGTTTT CAGAACCGGG AGATCACGTC GGAGGTGTAG TACTCCATCG GCACCAGCGA ATGCGGTGCC GGCCGGCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA