MythA.00185.b

Acyl-CoA dehydrogenase (A0QTF4 homolog)

CENTER ID: MythA.00185.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MythA.00185.b.A1.GE28713 Full length( MythA.00185.b ) 1 444
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MythA.00185.b.A1.PS00649 Full length( MythA.00185.b ) 1 444

Structures

3PFD
DEPOSITED: 10/28/2010
DETERMINATION: XRay
CLONE: MythA.00185.b.A1.GE28713
PROTEIN: MythA.00185.b.A1.PS00649

Publications by SSGCID

SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1078020.3.peg.4491
RefSeq: WP_003928003.1
UniProt: G7CNE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAWSGNPSF ELFQLPEEHI ALREAIRALA EKEIAPYAAE VDEKARFPEE ALAALNSSGF SAIHVPEEYG GQGADSVATC IVIEEVARVD CSASLIPAVN KLGTMGLILR GSEELKKQVL PAVASGEAMA SYALSEREAG SDAASMRTRA VADGDDWILN GSKCWITNGG KSTWYTVMAV TDPDKGANGI SAFMVHKDDE GFTVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKERKQF GRPVSDNQGV QFMLADMAMK IEAARLMVYS AAARAERGEG DLGFISAASK CFASDVXQGG HHRRGPVVRR LRLHPGLPGR ADDARRQDHP DLRGHQPDSA GGHVPGAAQV IPRVLSRRER ACLYVAECXX XXXXXXXXXX XXXXXXXXXX QGRENAEIRT VHAR
NT Sequence
ATGGCAGCGT GGAGCGGTAA CCCGTCATTC GAGTTGTTCC AATTGCCCGA GGAGCACATC GCCCTGCGGG AGGCGATCCG GGCGCTCGCC GAGAAGGAGA TCGCCCCGTA CGCGGCCGAG GTCGACGAGA AGGCCCGGTT CCCCGAGGAG GCGTTGGCGG CGTTGAACTC GTCGGGGTTC AGCGCGATCC ACGTGCCCGA GGAGTACGGC GGACAGGGTG CGGACTCGGT GGCGACCTGC ATCGTGATCG AAGAGGTGGC CCGGGTGGAC TGTTCGGCGT CGCTGATCCC GGCGGTCAAC AAACTGGGCA CGATGGGGCT GATCCTGCGC GGCTCCGAGG AACTCAAGAA GCAGGTGCTG CCGGCGGTGG CCTCCGGGGA GGCGATGGCG TCATACGCGT TGTCGGAGCG GGAGGCCGGC AGCGACGCGG CGTCGATGCG GACCCGCGCC GTCGCCGACG GCGACGACTG GATTCTCAAC GGGTCCAAAT GCTGGATCAC CAACGGCGGC AAGTCGACCT GGTACACCGT GATGGCGGTG ACCGATCCGG ACAAGGGCGC CAACGGGATC TCGGCGTTCA TGGTGCACAA GGACGATGAG GGCTTCACCG TCGGCCCCAA GGAACGCAAG CTCGGCATCA AGGGGTCGCC GACCACCGAG TTGTACTTCG AGAACTGTCG CATCCCGGGC GACCGGATCA TCGGTGAGCC CGGAACCGGG TTCAAGACCG CCCTGGCCAC CCTCGACCAC ACCCGGCCCA CCATCGGCGC CCAGGCAGTC GGGATCGCCC AGGGCGCGCT CGACGCGGCG ATCGCCTACA CCAAGGAGCG CAAGCAGTTC GGCCGCCCGG TCAGCGACAA CCAGGGTGTG CAGTTCATGC TCGCCGACAT GGCGATGAAG ATCGAGGCGG CCCGGTTGAT GGTGTACTCG GCGGCCGCGC GCGCCGAACG CGGTGAGGGC GACCTCGGGT TCATCTCGGC GGCGTCGAAG TGTTTCGCCT CCGACGTGGN CCAGGGGGGT CACCACCGAC GCGGTCCAGT TGTTCGGCGG TTACGGCTAC ACCCAGGACT TCCCGGTCGA GCGGATGATG CGCGACGCCA AGATCACCCA GATCTACGAG GGCACCAACC AGATTCAGCG GGTGGTCATG TCCCGGGCGC TGCTCAGGTA ATCCCGCGCG TGCTCTCCCG CCGCGAGCGG GCGTGTCTGT ATGTAGCCGA GTGTGNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CAGGGGCGCG AAAACGCCGA AATCCGCACC GTGCACGCAC GT
Details for MythA.00185.b.A1.GE28713
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 50kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 89
PERCENT COVERAGE: 83
Validated AA Sequence
MAAWSGNPSF ELFQLPEEHI ALREAIRALA EKEIAPYAAE VDEKARFPEE ALAALNSSGF SAIHVPEEYG GQGADSVATC IVIEEVARVD CSASLIPAVN KLGTMGLILR GSEELKKQVL PAVASGEAMA SYALSEREAG SDAASMRTRA VADGDDWILN GSKCWITNGG KSTWYTVMAV TDPDKGANGI SAFMVHKDDE GFTVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKERKQF GRPVSDNQGV QFMLADMAMK IEAARLMVYS AAARAERGEG DLGFISAASK CFASDVAMEV TTDAVQLFGG YGYTQDFPVE RMMRDAKITQ IYEGTNQIQR VVMSRALLR
Validated NT Sequence
ttattaacgt gcgtgcacgg tgcggatttc ggcgttttcg cgcacctgcc gcacgatgac cgccgtcacc gcacaacgaa tacggctacc gcacagtgag tacggccgcc gcacactcgg ctacatacag acacgcccgc tcgcggcggg agagcacgcg cgggattacc tgagcagcgc ccgggacatg accacccgct gaatctggtt ggtgccctcg tagatctggg tgatcttggc gtcgcgcatc atccgctcga ccgggaagtc ctgggtgtag ccgtaaccgc cgaacaactg gaccgcgtcg gtggtgacct ccatggccac gtcggaggcg aaacacttcg acgccgccga gatgaacccg aggtcgccct caccgcgttc ggcgcgcgcg gccgccgagt acaccatcaa ccgggccgcc tcgatcttca tcgccatgtc ggcgagcatg aactgcacac cctggttgtc gctgaccggg cggccgaact gcttgcgctc cttggtgtag gcgatcgccg cgtcgagcgc gccctgggcg atcccgactg cctgggcgcc gatggtgggc cgggtgtggt cgagggtggc cagggcggtc ttgaacccgg ttccgggctc accgatgatc cggtcgcccg ggatgcgaca gttctcgaag tacaactcgg tggtcggcga ccccttgatg ccgagcttgc gttccttggg gccgacggtg aagccctcat cgtccttgtg caccatgaac gccgagatcc cgttggcgcc cttgtccgga tcggtcaccg ccatcacggt gtaccaggtc gacttgccgc cgttggtgat ccagcatttg gacccgttga gaatccagtc gtcgccgtcg gcgacggcgc gggtccgcat cgacgccgcg tcgctgccgg cctcccgctc cgacaacgcg tatgacgcca tcgcctcccc ggaggccacc gccggcagca cctgcttctt gagttcctcg gagccgcgca ggatcagccc catcgtgccc agtttgttga ccgccgggat cagcgacgcc gaacagtcca cccgggccac ctcttcgatc acgatgcagg tcgccaccga gtccgcaccc tgtccgccgt actcctcggg cacgtggatc gcgctgaacc ccgacgagtt caacgccgcc aacgcctcct cggggaaccg ggccttctcg tcgacctcgg ccgcgtacgg ggcgatctcc ttctcggcga gcgcccggat cgcctcccgc agggcgatgt gctcctcggg caattggaac aactcgaatg acgggttacc gctccacgct gccatcgaac cagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWSGNPS FELFQLPEEH IALREAIRAL AEKEIAPYAA EVDEKARFPE EALAALNSSG FSAIHVPEEY GGQGADSVAT CIVIEEVARV DCSASLIPAV NKLGTMGLIL RGSEELKKQV LPAVASGEAM ASYALSEREA GSDAASMRTR AVADGDDWIL NGSKCWITNG GKSTWYTVMA VTDPDKGANG ISAFMVHKDD EGFTVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKERKQ FGRPVSDNQG VQFMLADMAM KIEAARLMVY SAAARAERGE GDLGFISAAS KCFASDVXQG GHHRRGPVVR RLRLHPGLPG RADDARRQDH PDLRGHQPDS AGGHVPGAAQ VIPRVLSRRE RACLYVAECX XXXXXXXXXX XXXXXXXXXX XQGRENAEIR TVHAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagcgtgg agcggtaacc cgtcattcga gttgttccaa ttgcccgagg agcacatcgc cctgcgggag gcgatccggg cgctcgccga gaaggagatc gccccgtacg cggccgaggt cgacgagaag gcccggttcc ccgaggaggc gttggcggcg ttgaactcgt cggggttcag cgcgatccac gtgcccgagg agtacggcgg acagggtgcg gactcggtgg cgacctgcat cgtgatcgaa gaggtggccc gggtggactg ttcggcgtcg ctgatcccgg cggtcaacaa actgggcacg atggggctga tcctgcgcgg ctccgaggaa ctcaagaagc aggtgctgcc ggcggtggcc tccggggagg cgatggcgtc atacgcgttg tcggagcggg aggccggcag cgacgcggcg tcgatgcgga cccgcgccgt cgccgacggc gacgactgga ttctcaacgg gtccaaatgc tggatcacca acggcggcaa gtcgacctgg tacaccgtga tggcggtgac cgatccggac aagggcgcca acgggatctc ggcgttcatg gtgcacaagg acgatgaggg cttcaccgtc ggccccaagg aacgcaagct cggcatcaag gggtcgccga ccaccgagtt gtacttcgag aactgtcgca tcccgggcga ccggatcatc ggtgagcccg gaaccgggtt caagaccgcc ctggccaccc tcgaccacac ccggcccacc atcggcgccc aggcagtcgg gatcgcccag ggcgcgctcg acgcggcgat cgcctacacc aaggagcgca agcagttcgg ccgcccggtc agcgacaacc agggtgtgca gttcatgctc gccgacatgg cgatgaagat cgaggcggcc cggttgatgg tgtactcggc ggccgcgcgc gccgaacgcg gtgagggcga cctcgggttc atctcggcgg cgtcgaagtg tttcgcctcc gacgtggncc aggggggtca ccaccgacgc ggtccagttg ttcggcggtt acggctacac ccaggacttc ccggtcgagc ggatgatgcg cgacgccaag atcacccaga tctacgaggg caccaaccag attcagcggg tggtcatgtc ccgggcgctg ctcaggtaat cccgcgcgtg ctctcccgcc gcgagcgggc gtgtctgtat gtagccgagt gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca ggggcgcgaa aacgccgaaa tccgcaccgt gcacgcacgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MythA.00185.b.A1.PS00649
PURIFICATION DATe: 6/25/2010
CONCENTRATION: 116.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 89
PERCENT COVERAGE: 83
Protocol Notes
notes unavailable
Validated AA Sequence
MAAWSGNPSF ELFQLPEEHI ALREAIRALA EKEIAPYAAE VDEKARFPEE ALAALNSSGF SAIHVPEEYG GQGADSVATC IVIEEVARVD CSASLIPAVN KLGTMGLILR GSEELKKQVL PAVASGEAMA SYALSEREAG SDAASMRTRA VADGDDWILN GSKCWITNGG KSTWYTVMAV TDPDKGANGI SAFMVHKDDE GFTVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKERKQF GRPVSDNQGV QFMLADMAMK IEAARLMVYS AAARAERGEG DLGFISAASK CFASDVAMEV TTDAVQLFGG YGYTQDFPVE RMMRDAKITQ IYEGTNQIQR VVMSRALLR
Validated NT Sequence
ttattaacgt gcgtgcacgg tgcggatttc ggcgttttcg cgcacctgcc gcacgatgac cgccgtcacc gcacaacgaa tacggctacc gcacagtgag tacggccgcc gcacactcgg ctacatacag acacgcccgc tcgcggcggg agagcacgcg cgggattacc tgagcagcgc ccgggacatg accacccgct gaatctggtt ggtgccctcg tagatctggg tgatcttggc gtcgcgcatc atccgctcga ccgggaagtc ctgggtgtag ccgtaaccgc cgaacaactg gaccgcgtcg gtggtgacct ccatggccac gtcggaggcg aaacacttcg acgccgccga gatgaacccg aggtcgccct caccgcgttc ggcgcgcgcg gccgccgagt acaccatcaa ccgggccgcc tcgatcttca tcgccatgtc ggcgagcatg aactgcacac cctggttgtc gctgaccggg cggccgaact gcttgcgctc cttggtgtag gcgatcgccg cgtcgagcgc gccctgggcg atcccgactg cctgggcgcc gatggtgggc cgggtgtggt cgagggtggc cagggcggtc ttgaacccgg ttccgggctc accgatgatc cggtcgcccg ggatgcgaca gttctcgaag tacaactcgg tggtcggcga ccccttgatg ccgagcttgc gttccttggg gccgacggtg aagccctcat cgtccttgtg caccatgaac gccgagatcc cgttggcgcc cttgtccgga tcggtcaccg ccatcacggt gtaccaggtc gacttgccgc cgttggtgat ccagcatttg gacccgttga gaatccagtc gtcgccgtcg gcgacggcgc gggtccgcat cgacgccgcg tcgctgccgg cctcccgctc cgacaacgcg tatgacgcca tcgcctcccc ggaggccacc gccggcagca cctgcttctt gagttcctcg gagccgcgca ggatcagccc catcgtgccc agtttgttga ccgccgggat cagcgacgcc gaacagtcca cccgggccac ctcttcgatc acgatgcagg tcgccaccga gtccgcaccc tgtccgccgt actcctcggg cacgtggatc gcgctgaacc ccgacgagtt caacgccgcc aacgcctcct cggggaaccg ggccttctcg tcgacctcgg ccgcgtacgg ggcgatctcc ttctcggcga gcgcccggat cgcctcccgc agggcgatgt gctcctcggg caattggaac aactcgaatg acgggttacc gctccacgct gccatcgaac cagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWSGNPS FELFQLPEEH IALREAIRAL AEKEIAPYAA EVDEKARFPE EALAALNSSG FSAIHVPEEY GGQGADSVAT CIVIEEVARV DCSASLIPAV NKLGTMGLIL RGSEELKKQV LPAVASGEAM ASYALSEREA GSDAASMRTR AVADGDDWIL NGSKCWITNG GKSTWYTVMA VTDPDKGANG ISAFMVHKDD EGFTVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKERKQ FGRPVSDNQG VQFMLADMAM KIEAARLMVY SAAARAERGE GDLGFISAAS KCFASDVXQG GHHRRGPVVR RLRLHPGLPG RADDARRQDH PDLRGHQPDS AGGHVPGAAQ VIPRVLSRRE RACLYVAECX XXXXXXXXXX XXXXXXXXXX XQGRENAEIR TVHAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGCGTGG AGCGGTAACC CGTCATTCGA GTTGTTCCAA TTGCCCGAGG AGCACATCGC CCTGCGGGAG GCGATCCGGG CGCTCGCCGA GAAGGAGATC GCCCCGTACG CGGCCGAGGT CGACGAGAAG GCCCGGTTCC CCGAGGAGGC GTTGGCGGCG TTGAACTCGT CGGGGTTCAG CGCGATCCAC GTGCCCGAGG AGTACGGCGG ACAGGGTGCG GACTCGGTGG CGACCTGCAT CGTGATCGAA GAGGTGGCCC GGGTGGACTG TTCGGCGTCG CTGATCCCGG CGGTCAACAA ACTGGGCACG ATGGGGCTGA TCCTGCGCGG CTCCGAGGAA CTCAAGAAGC AGGTGCTGCC GGCGGTGGCC TCCGGGGAGG CGATGGCGTC ATACGCGTTG TCGGAGCGGG AGGCCGGCAG CGACGCGGCG TCGATGCGGA CCCGCGCCGT CGCCGACGGC GACGACTGGA TTCTCAACGG GTCCAAATGC TGGATCACCA ACGGCGGCAA GTCGACCTGG TACACCGTGA TGGCGGTGAC CGATCCGGAC AAGGGCGCCA ACGGGATCTC GGCGTTCATG GTGCACAAGG ACGATGAGGG CTTCACCGTC GGCCCCAAGG AACGCAAGCT CGGCATCAAG GGGTCGCCGA CCACCGAGTT GTACTTCGAG AACTGTCGCA TCCCGGGCGA CCGGATCATC GGTGAGCCCG GAACCGGGTT CAAGACCGCC CTGGCCACCC TCGACCACAC CCGGCCCACC ATCGGCGCCC AGGCAGTCGG GATCGCCCAG GGCGCGCTCG ACGCGGCGAT CGCCTACACC AAGGAGCGCA AGCAGTTCGG CCGCCCGGTC AGCGACAACC AGGGTGTGCA GTTCATGCTC GCCGACATGG CGATGAAGAT CGAGGCGGCC CGGTTGATGG TGTACTCGGC GGCCGCGCGC GCCGAACGCG GTGAGGGCGA CCTCGGGTTC ATCTCGGCGG CGTCGAAGTG TTTCGCCTCC GACGTGGNCC AGGGGGGTCA CCACCGACGC GGTCCAGTTG TTCGGCGGTT ACGGCTACAC CCAGGACTTC CCGGTCGAGC GGATGATGCG CGACGCCAAG ATCACCCAGA TCTACGAGGG CACCAACCAG ATTCAGCGGG TGGTCATGTC CCGGGCGCTG CTCAGGTAAT CCCGCGCGTG CTCTCCCGCC GCGAGCGGGC GTGTCTGTAT GTAGCCGAGT GTGNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGCA GGGGCGCGAA AACGCCGAAA TCCGCACCGT GCACGCACGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA