MythA.00185.b
Acyl-CoA dehydrogenase (A0QTF4 homolog)
CENTER ID:
MythA.00185.b
ORGANISM:
Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:
Respiratory Infection
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
False
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIC
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
MythA.00185.b.A1.GE28713
Full length( MythA.00185.b )
info
1
444
MythA.00185.b.A1.GE28713
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
MythA.00185.b.A1.PS00649
Full length( MythA.00185.b )
info
1
444
PS00649
Structures
3PFD
DEPOSITED: 10/28/2010
DETERMINATION: XRay
CLONE: MythA.00185.b.A1.GE28713
PROTEIN: MythA.00185.b.A1.PS00649
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PDB:
3K9G ,
3KM3 ,
3KW3 ,
3LUZ ,
3MEN ,
3NJB ,
3O2E ,
3OIB ,
3P96 ,
3PFD ,
3PM6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAWSGNPSF ELFQLPEEHI ALREAIRALA EKEIAPYAAE VDEKARFPEE ALAALNSSGF SAIHVPEEYG GQGADSVATC IVIEEVARVD CSASLIPAVN KLGTMGLILR GSEELKKQVL PAVASGEAMA SYALSEREAG SDAASMRTRA VADGDDWILN GSKCWITNGG KSTWYTVMAV TDPDKGANGI SAFMVHKDDE GFTVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKERKQF GRPVSDNQGV QFMLADMAMK IEAARLMVYS AAARAERGEG DLGFISAASK CFASDVXQGG HHRRGPVVRR LRLHPGLPGR ADDARRQDHP DLRGHQPDSA GGHVPGAAQV IPRVLSRRER ACLYVAECXX XXXXXXXXXX XXXXXXXXXX QGRENAEIRT VHAR
NT Sequence
ATGGCAGCGT GGAGCGGTAA CCCGTCATTC GAGTTGTTCC AATTGCCCGA GGAGCACATC GCCCTGCGGG AGGCGATCCG GGCGCTCGCC GAGAAGGAGA TCGCCCCGTA CGCGGCCGAG GTCGACGAGA AGGCCCGGTT CCCCGAGGAG GCGTTGGCGG CGTTGAACTC GTCGGGGTTC AGCGCGATCC ACGTGCCCGA GGAGTACGGC GGACAGGGTG CGGACTCGGT GGCGACCTGC ATCGTGATCG AAGAGGTGGC CCGGGTGGAC TGTTCGGCGT CGCTGATCCC GGCGGTCAAC AAACTGGGCA CGATGGGGCT GATCCTGCGC GGCTCCGAGG AACTCAAGAA GCAGGTGCTG CCGGCGGTGG CCTCCGGGGA GGCGATGGCG TCATACGCGT TGTCGGAGCG GGAGGCCGGC AGCGACGCGG CGTCGATGCG GACCCGCGCC GTCGCCGACG GCGACGACTG GATTCTCAAC GGGTCCAAAT GCTGGATCAC CAACGGCGGC AAGTCGACCT GGTACACCGT GATGGCGGTG ACCGATCCGG ACAAGGGCGC CAACGGGATC TCGGCGTTCA TGGTGCACAA GGACGATGAG GGCTTCACCG TCGGCCCCAA GGAACGCAAG CTCGGCATCA AGGGGTCGCC GACCACCGAG TTGTACTTCG AGAACTGTCG CATCCCGGGC GACCGGATCA TCGGTGAGCC CGGAACCGGG TTCAAGACCG CCCTGGCCAC CCTCGACCAC ACCCGGCCCA CCATCGGCGC CCAGGCAGTC GGGATCGCCC AGGGCGCGCT CGACGCGGCG ATCGCCTACA CCAAGGAGCG CAAGCAGTTC GGCCGCCCGG TCAGCGACAA CCAGGGTGTG CAGTTCATGC TCGCCGACAT GGCGATGAAG ATCGAGGCGG CCCGGTTGAT GGTGTACTCG GCGGCCGCGC GCGCCGAACG CGGTGAGGGC GACCTCGGGT TCATCTCGGC GGCGTCGAAG TGTTTCGCCT CCGACGTGGN CCAGGGGGGT CACCACCGAC GCGGTCCAGT TGTTCGGCGG TTACGGCTAC ACCCAGGACT TCCCGGTCGA GCGGATGATG CGCGACGCCA AGATCACCCA GATCTACGAG GGCACCAACC AGATTCAGCG GGTGGTCATG TCCCGGGCGC TGCTCAGGTA ATCCCGCGCG TGCTCTCCCG CCGCGAGCGG GCGTGTCTGT ATGTAGCCGA GTGTGNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CAGGGGCGCG AAAACGCCGA AATCCGCACC GTGCACGCAC GT
Details for MythA.00185.b.A1.GE28713
HARVESTED ON:
3/1/2010
SEQUENCED ON:
3/5/2010
EXPECTED MW:
50kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Few (1-10)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with sequence variation
PERCENT IDENTITY:
89
PERCENT COVERAGE:
83
Details for MythA.00185.b.A1.PS00649
PURIFICATION DATe:
6/25/2010
CONCENTRATION:
116.1mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
200µl
PERCENT IDENTITY:
89
PERCENT COVERAGE:
83