MysmA.00185.c

Acyl-CoA dehydrogenase

CENTER ID: MysmA.00185.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00185.c.A1.GE28579 Full length( MysmA.00185.c ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00185.c.A1.PS00541 Full length( MysmA.00185.c ) 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1802
RefSeq: YP_886192.1
UniProt: A0QTF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAWGGNPSF DLFQLPEEHQ ELRAAIRALA EKEIAPHAAD VDENARFPQE ALDALNASGF NAVHVPEEYG GQGADSVAAC IVIEEVARVD ASASLIPAVN KLGTMGLILR GSDELKKQVL PQLASGAAMA SYALSEREAG SDAASMRTRA RADGDDWVLN GTKCWITNGG KSSWYTVMAV TDPDKGANGI SAFMVHQDDE GFSVGPKERK LGIKGSPTTE LYFENCRIPG DRIIGEPGTG FKTALATLDH TRPTIGAQAV GIAQGALDAA IAYTKDRKQF GTSISDFQAV QFMLADMAMK VESARLMVYH AAARAERGEK NLGFISAASK CLASDIAMEV TTDAVQLFGG AGYTQDFPVE RMMRDAKITQ IYEGTNQIQR VVMSRALLR
NT Sequence
ATGGCTGCAT GGGGCGGCAA TCCGTCTTTC GATCTGTTCC AGTTGCCCGA GGAGCATCAG GAGTTGCGGG CGGCGATCCG TGCGCTGGCT GAGAAGGAGA TCGCGCCGCA CGCTGCCGAT GTGGATGAGA ATGCGCGGTT TCCGCAGGAG GCGCTCGATG CGTTGAATGC TTCGGGGTTC AACGCGGTGC ACGTGCCTGA GGAGTACGGC GGTCAGGGCG CGGATTCGGT CGCGGCGTGC ATCGTGATCG AGGAGGTCGC GCGGGTGGAT GCCTCGGCGT CGTTGATCCC GGCGGTCAAC AAGCTGGGCA CGATGGGGTT GATCCTGCGT GGTTCGGATG AGTTGAAGAA GCAGGTGTTG CCGCAGTTGG CCTCGGGTGC GGCGATGGCG TCGTATGCGT TGTCCGAGCG TGAGGCCGGC AGTGATGCGG CGAGTATGCG CACGCGGGCC AGGGCCGATG GGGATGACTG GGTTCTCAAC GGCACCAAGT GCTGGATCAC CAATGGTGGT AAGTCGTCGT GGTACACGGT GATGGCGGTG ACCGATCCGG ACAAGGGTGC CAACGGGATT TCGGCGTTCA TGGTGCACCA GGACGACGAG GGGTTCAGCG TCGGTCCCAA GGAGCGCAAG CTGGGCATCA AGGGGTCGCC GACCACGGAG TTGTATTTCG AGAACTGTCG GATTCCCGGG GATCGGATCA TCGGTGAGCC GGGTACGGGG TTCAAGACGG CGTTGGCGAC GTTGGATCAC ACGCGCCCGA CGATCGGGGC GCAGGCGGTG GGGATCGCCC AGGGTGCGTT GGATGCGGCG ATCGCCTACA CCAAGGACCG CAAGCAGTTC GGTACGAGCA TCAGTGATTT TCAGGCTGTG CAGTTCATGT TGGCCGATAT GGCGATGAAG GTCGAGTCGG CGCGGTTGAT GGTGTATCAC GCCGCGGCGC GTGCTGAGCG TGGTGAGAAG AACCTGGGGT TCATCTCGGC GGCCAGTAAG TGTCTGGCGT CGGATATCGC CATGGAGGTC ACCACCGATG CGGTGCAGTT GTTCGGCGGT GCGGGTTATA CGCAGGACTT CCCGGTGGAG CGGATGATGC GTGATGCCAA GATCACCCAG ATCTATGAGG GCACCAACCA GATCCAGCGC GTGGTCATGA GCCGCGCCCT GCTGCGC
Details for MysmA.00185.c.A1.GE28579
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWGGNPS FDLFQLPEEH QELRAAIRAL AEKEIAPHAA DVDENARFPQ EALDALNASG FNAVHVPEEY GGQGADSVAA CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSDELKKQV LPQLASGAAM ASYALSEREA GSDAASMRTR ARADGDDWVL NGTKCWITNG GKSSWYTVMA VTDPDKGANG ISAFMVHQDD EGFSVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKDRKQ FGTSISDFQA VQFMLADMAM KVESARLMVY HAAARAERGE KNLGFISAAS KCLASDIAME VTTDAVQLFG GAGYTQDFPV ERMMRDAKIT QIYEGTNQIQ RVVMSRALLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg catggggcgg caatccgtct ttcgatctgt tccagttgcc cgaggagcat caggagttgc gggcggcgat ccgtgcactg gctgagaagg agatcgcgcc gcacgctgcc gatgtggatg agaatgcgcg gtttccgcag gaggcgctcg atgcgttgaa tgcttcgggg ttcaacgcgg tgcacgtgcc tgaggagtac ggcggtcagg gcgcggattc ggtcgcggcg tgcatcgtga tcgaggaggt cgcgcgggtg gatgcctcgg cgtcgttgat cccggcggtc aacaagctgg gcacgatggg gttgatcctg cgtggttcgg atgagttgaa gaagcaggtg ttgccgcagt tggcctcggg tgcggcgatg gcgtcgtatg cgttgtccga gcgtgaggcc ggcagtgatg cggcgagtat gcgcacgcgg gccagggccg atggggatga ctgggttctc aacggcacca agtgctggat caccaatggt ggtaagtcgt cgtggtacac ggtgatggcg gtgaccgatc cggacaaggg tgccaacggg atttcggcgt tcatggtgca ccaggacgac gaggggttca gcgtcggtcc caaggagcgc aagctgggca tcaaggggtc gccgaccacg gagttgtatt tcgagaactg tcggattccc ggggatcgga tcatcggtga gccgggtacg gggttcaaga cggcgttggc gacgttggat cacacgcgcc cgacgatcgg ggcgcaggcg gtggggatcg cccagggtgc gttggatgcg gcgatcgcct acaccaagga ccgcaagcag ttcggtacga gcatcagtga ttttcaggct gtgcagttca tgttggccga tatggcgatg aaggtcgagt cggcgcggtt gatggtgtat cacgccgcgg cgcgtgctga gcgtggtgag aagaacctgg ggttcatctc ggcggccagt aagtgtctgg cgtcggatat cgccatggag gtcaccaccg atgcggtgca gttgttcggc ggtgcgggtt atacgcagga cttcccggtg gagcggatga tgcgtgatgc caagatcacc cagatctatg agggcaccaa ccagatccag cgcgtggtca tgagccgcgc cctgctgcgc taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWGGNPS FDLFQLPEEH QELRAAIRAL AEKEIAPHAA DVDENARFPQ EALDALNASG FNAVHVPEEY GGQGADSVAA CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSDELKKQV LPQLASGAAM ASYALSEREA GSDAASMRTR ARADGDDWVL NGTKCWITNG GKSSWYTVMA VTDPDKGANG ISAFMVHQDD EGFSVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKDRKQ FGTSISDFQA VQFMLADMAM KVESARLMVY HAAARAERGE KNLGFISAAS KCLASDIAME VTTDAVQLFG GAGYTQDFPV ERMMRDAKIT QIYEGTNQIQ RVVMSRALLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcatgg ggcggcaatc cgtctttcga tctgttccag ttgcccgagg agcatcagga gttgcgggcg gcgatccgtg cgctggctga gaaggagatc gcgccgcacg ctgccgatgt ggatgagaat gcgcggtttc cgcaggaggc gctcgatgcg ttgaatgctt cggggttcaa cgcggtgcac gtgcctgagg agtacggcgg tcagggcgcg gattcggtcg cggcgtgcat cgtgatcgag gaggtcgcgc gggtggatgc ctcggcgtcg ttgatcccgg cggtcaacaa gctgggcacg atggggttga tcctgcgtgg ttcggatgag ttgaagaagc aggtgttgcc gcagttggcc tcgggtgcgg cgatggcgtc gtatgcgttg tccgagcgtg aggccggcag tgatgcggcg agtatgcgca cgcgggccag ggccgatggg gatgactggg ttctcaacgg caccaagtgc tggatcacca atggtggtaa gtcgtcgtgg tacacggtga tggcggtgac cgatccggac aagggtgcca acgggatttc ggcgttcatg gtgcaccagg acgacgaggg gttcagcgtc ggtcccaagg agcgcaagct gggcatcaag gggtcgccga ccacggagtt gtatttcgag aactgtcgga ttcccgggga tcggatcatc ggtgagccgg gtacggggtt caagacggcg ttggcgacgt tggatcacac gcgcccgacg atcggggcgc aggcggtggg gatcgcccag ggtgcgttgg atgcggcgat cgcctacacc aaggaccgca agcagttcgg tacgagcatc agtgattttc aggctgtgca gttcatgttg gccgatatgg cgatgaaggt cgagtcggcg cggttgatgg tgtatcacgc cgcggcgcgt gctgagcgtg gtgagaagaa cctggggttc atctcggcgg ccagtaagtg tctggcgtcg gatatcgcca tggaggtcac caccgatgcg gtgcagttgt tcggcggtgc gggttatacg caggacttcc cggtggagcg gatgatgcgt gatgccaaga tcacccagat ctatgagggc accaaccaga tccagcgcgt ggtcatgagc cgcgccctgc tgcgcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.00185.c.A1.PS00541
PURIFICATION DATe: 3/30/2010
CONCENTRATION: 25.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWGGNPS FDLFQLPEEH QELRAAIRAL AEKEIAPHAA DVDENARFPQ EALDALNASG FNAVHVPEEY GGQGADSVAA CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSDELKKQV LPQLASGAAM ASYALSEREA GSDAASMRTR ARADGDDWVL NGTKCWITNG GKSSWYTVMA VTDPDKGANG ISAFMVHQDD EGFSVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKDRKQ FGTSISDFQA VQFMLADMAM KVESARLMVY HAAARAERGE KNLGFISAAS KCLASDIAME VTTDAVQLFG GAGYTQDFPV ERMMRDAKIT QIYEGTNQIQ RVVMSRALLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg catggggcgg caatccgtct ttcgatctgt tccagttgcc cgaggagcat caggagttgc gggcggcgat ccgtgcactg gctgagaagg agatcgcgcc gcacgctgcc gatgtggatg agaatgcgcg gtttccgcag gaggcgctcg atgcgttgaa tgcttcgggg ttcaacgcgg tgcacgtgcc tgaggagtac ggcggtcagg gcgcggattc ggtcgcggcg tgcatcgtga tcgaggaggt cgcgcgggtg gatgcctcgg cgtcgttgat cccggcggtc aacaagctgg gcacgatggg gttgatcctg cgtggttcgg atgagttgaa gaagcaggtg ttgccgcagt tggcctcggg tgcggcgatg gcgtcgtatg cgttgtccga gcgtgaggcc ggcagtgatg cggcgagtat gcgcacgcgg gccagggccg atggggatga ctgggttctc aacggcacca agtgctggat caccaatggt ggtaagtcgt cgtggtacac ggtgatggcg gtgaccgatc cggacaaggg tgccaacggg atttcggcgt tcatggtgca ccaggacgac gaggggttca gcgtcggtcc caaggagcgc aagctgggca tcaaggggtc gccgaccacg gagttgtatt tcgagaactg tcggattccc ggggatcgga tcatcggtga gccgggtacg gggttcaaga cggcgttggc gacgttggat cacacgcgcc cgacgatcgg ggcgcaggcg gtggggatcg cccagggtgc gttggatgcg gcgatcgcct acaccaagga ccgcaagcag ttcggtacga gcatcagtga ttttcaggct gtgcagttca tgttggccga tatggcgatg aaggtcgagt cggcgcggtt gatggtgtat cacgccgcgg cgcgtgctga gcgtggtgag aagaacctgg ggttcatctc ggcggccagt aagtgtctgg cgtcggatat cgccatggag gtcaccaccg atgcggtgca gttgttcggc ggtgcgggtt atacgcagga cttcccggtg gagcggatga tgcgtgatgc caagatcacc cagatctatg agggcaccaa ccagatccag cgcgtggtca tgagccgcgc cctgctgcgc taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAWGGNPS FDLFQLPEEH QELRAAIRAL AEKEIAPHAA DVDENARFPQ EALDALNASG FNAVHVPEEY GGQGADSVAA CIVIEEVARV DASASLIPAV NKLGTMGLIL RGSDELKKQV LPQLASGAAM ASYALSEREA GSDAASMRTR ARADGDDWVL NGTKCWITNG GKSSWYTVMA VTDPDKGANG ISAFMVHQDD EGFSVGPKER KLGIKGSPTT ELYFENCRIP GDRIIGEPGT GFKTALATLD HTRPTIGAQA VGIAQGALDA AIAYTKDRKQ FGTSISDFQA VQFMLADMAM KVESARLMVY HAAARAERGE KNLGFISAAS KCLASDIAME VTTDAVQLFG GAGYTQDFPV ERMMRDAKIT QIYEGTNQIQ RVVMSRALLR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGCATGG GGCGGCAATC CGTCTTTCGA TCTGTTCCAG TTGCCCGAGG AGCATCAGGA GTTGCGGGCG GCGATCCGTG CGCTGGCTGA GAAGGAGATC GCGCCGCACG CTGCCGATGT GGATGAGAAT GCGCGGTTTC CGCAGGAGGC GCTCGATGCG TTGAATGCTT CGGGGTTCAA CGCGGTGCAC GTGCCTGAGG AGTACGGCGG TCAGGGCGCG GATTCGGTCG CGGCGTGCAT CGTGATCGAG GAGGTCGCGC GGGTGGATGC CTCGGCGTCG TTGATCCCGG CGGTCAACAA GCTGGGCACG ATGGGGTTGA TCCTGCGTGG TTCGGATGAG TTGAAGAAGC AGGTGTTGCC GCAGTTGGCC TCGGGTGCGG CGATGGCGTC GTATGCGTTG TCCGAGCGTG AGGCCGGCAG TGATGCGGCG AGTATGCGCA CGCGGGCCAG GGCCGATGGG GATGACTGGG TTCTCAACGG CACCAAGTGC TGGATCACCA ATGGTGGTAA GTCGTCGTGG TACACGGTGA TGGCGGTGAC CGATCCGGAC AAGGGTGCCA ACGGGATTTC GGCGTTCATG GTGCACCAGG ACGACGAGGG GTTCAGCGTC GGTCCCAAGG AGCGCAAGCT GGGCATCAAG GGGTCGCCGA CCACGGAGTT GTATTTCGAG AACTGTCGGA TTCCCGGGGA TCGGATCATC GGTGAGCCGG GTACGGGGTT CAAGACGGCG TTGGCGACGT TGGATCACAC GCGCCCGACG ATCGGGGCGC AGGCGGTGGG GATCGCCCAG GGTGCGTTGG ATGCGGCGAT CGCCTACACC AAGGACCGCA AGCAGTTCGG TACGAGCATC AGTGATTTTC AGGCTGTGCA GTTCATGTTG GCCGATATGG CGATGAAGGT CGAGTCGGCG CGGTTGATGG TGTATCACGC CGCGGCGCGT GCTGAGCGTG GTGAGAAGAA CCTGGGGTTC ATCTCGGCGG CCAGTAAGTG TCTGGCGTCG GATATCGCCA TGGAGGTCAC CACCGATGCG GTGCAGTTGT TCGGCGGTGC GGGTTATACG CAGGACTTCC CGGTGGAGCG GATGATGCGT GATGCCAAGA TCACCCAGAT CTATGAGGGC ACCAACCAGA TCCAGCGCGT GGTCATGAGC CGCGCCCTGC TGCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA