MysmA.00185.a

Acyl-CoA dehydrogenase

CENTER ID: MysmA.00185.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00185.a.A1.GE28577 Full length( MysmA.00185.a ) 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00185.a.A1.PS00554 Full length( MysmA.00185.a ) 1 377

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3921
RefSeq: YP_888269.1
UniProt: A0QZD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTELLDFRA MMQDLASTQV APYARDVDEQ ERFPEEAWQA LCAADLPGLA HPTELGGSDG DLAAQAIAVE ELSAACASTA MVLLVNWAGT CTVIHQGSDE LKKLVVPDVS TGAVGAGWCL TEPTGGSDLS GIRTKAQRDG SDWVLNGQKR FISNADWGDW FAVLARSGEE NEFGVFMVHK SDAGLSFGPP EKKMGLRGSP TADVILEDCR IPGERVVGDP TQGYKYIIDE LNGSRALIAA QALGLAKGAL RTAIEYTAER RQFGSALSTF QMVRAMVADA AIRVESASAL LYKAVDAIEN KDPRARSLVS MAKVLCSDNA MAVTTDAVQL LGGYGFTREY PVERMMRDAK VTQIYEGSNQ IQRLVISKGL YKGEALS
NT Sequence
ATGAACACAG AATTGCTGGA TTTCCGGGCG ATGATGCAAG ACCTGGCGTC CACCCAGGTG GCACCGTACG CGCGTGACGT CGACGAGCAG GAACGGTTCC CAGAAGAGGC GTGGCAGGCG CTCTGCGCCG CCGACCTGCC CGGACTTGCT CACCCCACTG AATTGGGTGG CAGTGACGGT GATCTCGCCG CCCAGGCGAT CGCGGTGGAA GAGCTCTCAG CCGCATGTGC CTCGACTGCG ATGGTCTTGC TGGTCAATTG GGCCGGCACC TGCACCGTCA TTCATCAGGG ATCCGACGAG CTCAAGAAGT TGGTGGTGCC CGATGTCTCC ACCGGTGCTG TGGGAGCGGG ATGGTGCTTG ACCGAACCCA CCGGCGGATC GGATCTCTCT GGTATTCGCA CCAAGGCGCA ACGTGACGGG TCCGATTGGG TTCTCAACGG CCAGAAGCGC TTCATCAGCA ACGCGGACTG GGGCGATTGG TTCGCGGTGC TGGCCCGTTC CGGTGAGGAG AACGAGTTCG GCGTATTCAT GGTCCACAAG AGCGACGCCG GCCTCAGCTT CGGGCCACCC GAGAAGAAGA TGGGACTTCG CGGAAGCCCC ACGGCTGATG TCATTCTCGA AGACTGCCGG ATCCCCGGCG AACGAGTCGT CGGGGATCCC ACCCAAGGCT ACAAATACAT CATCGACGAG TTGAACGGAA GTCGGGCTCT GATCGCGGCG CAGGCACTCG GTTTGGCAAA GGGCGCGCTT CGCACGGCGA TCGAATACAC CGCCGAGCGC CGCCAGTTCG GATCAGCGCT GTCGACATTT CAGATGGTGC GCGCCATGGT CGCCGACGCA GCGATCCGCG TCGAGTCCGC AAGTGCCCTG CTCTACAAGG CCGTCGACGC CATCGAGAAC AAAGATCCTC GCGCTCGTTC GCTGGTTTCC ATGGCAAAAG TGCTGTGTAG TGACAACGCC ATGGCGGTAA CGACAGACGC TGTTCAACTC CTCGGCGGTT ACGGGTTCAC GCGCGAGTAC CCCGTGGAGC GAATGATGCG AGACGCGAAG GTGACTCAGA TCTATGAGGG GAGTAATCAG ATCCAGCGTC TGGTGATCTC GAAGGGACTG TACAAAGGGG AGGCGCTGTC A
Details for MysmA.00185.a.A1.GE28577
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNTELLDFR AMMQDLASTQ VAPYARDVDE QERFPEEAWQ ALCAADLPGL AHPTELGGSD GDLAAQAIAV EELSAACAST AMVLLVNWAG TCTVIHQGSD ELKKLVVPDV STGAVGAGWC LTEPTGGSDL SGIRTKAQRD GSDWVLNGQK RFISNADWGD WFAVLARSGE ENEFGVFMVH KSDAGLSFGP PEKKMGLRGS PTADVILEDC RIPGERVVGD PTQGYKYIID ELNGSRALIA AQALGLAKGA LRTAIEYTAE RRQFGSALST FQMVRAMVAD AAIRVESASA LLYKAVDAIE NKDPRARSLV SMAKVLCSDN AMAVTTDAVQ LLGGYGFTRE YPVERMMRDA KVTQIYEGSN QIQRLVISKG LYKGEALS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca cagaattgct ggatttccgg gcgatgatgc aagacctggc gtccacccag gtggcaccgt acgcgcgtga cgtcgacgag caggaacggt tcccagaaga ggcgtggcag gcgctctgcg ccgccgacct gcccggactt gctcacccca ctgaattggg tggcagtgac ggtgatctcg ccgcccaggc gatcgcggtg gaagagctct cagccgcatg tgcctcgact gcgatggtct tgctggtcaa ttgggccggc acctgcaccg tcattcatca gggatccgac gagctcaaga agttggtggt gcccgatgtc tccaccggtg ctgtgggagc gggatggtgc ttgaccgaac ccaccggcgg atcggatctc tctggtattc gcaccaaggc gcaacgtgac gggtccgatt gggttctcaa cggccagaag cgcttcatca gcaacgcgga ctggggcgat tggttcgcgg tgctggcccg ttccggtgag gagaacgagt tcggcgtatt catggtccac aagagcgacg ccggcctcag cttcgggcca cccgagaaga agatgggact tcgcggaagc cccacggctg atgtcattct cgaagactgc cggatccccg gcgaacgagt cgtcggggat cccacccaag gctacaaata catcatcgac gagttgaacg gaagtcgggc tctgatcgcg gcgcaggcac tcggtttggc aaagggcgcg cttcgcacgg cgatcgaata caccgccgag cgccgccagt tcggatcagc gctgtcgaca tttcagatgg tgcgcgccat ggtcgccgac gcagcgatcc gcgtcgagtc cgcaagtgcc ctgctctaca aggccgtcga cgccatcgag aacaaagatc ctcgcgctcg ttcgctggtt tccatggcaa aagtgctgtg tagtgacaac gccatggcgg taacgacaga cgctgttcaa ctcctcggcg gttacgggtt cacgcgcgag taccccgtgg agcgaatgat gcgagacgcg aaggtgactc agatctatga ggggagtaat cagatccagc gtctggtgat ctcgaaggga ctgtacaaag gggaggcgct gtcataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTELLDFR AMMQDLASTQ VAPYARDVDE QERFPEEAWQ ALCAADLPGL AHPTELGGSD GDLAAQAIAV EELSAACAST AMVLLVNWAG TCTVIHQGSD ELKKLVVPDV STGAVGAGWC LTEPTGGSDL SGIRTKAQRD GSDWVLNGQK RFISNADWGD WFAVLARSGE ENEFGVFMVH KSDAGLSFGP PEKKMGLRGS PTADVILEDC RIPGERVVGD PTQGYKYIID ELNGSRALIA AQALGLAKGA LRTAIEYTAE RRQFGSALST FQMVRAMVAD AAIRVESASA LLYKAVDAIE NKDPRARSLV SMAKVLCSDN AMAVTTDAVQ LLGGYGFTRE YPVERMMRDA KVTQIYEGSN QIQRLVISKG LYKGEALS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacacagaa ttgctggatt tccgggcgat gatgcaagac ctggcgtcca cccaggtggc accgtacgcg cgtgacgtcg acgagcagga acggttccca gaagaggcgt ggcaggcgct ctgcgccgcc gacctgcccg gacttgctca ccccactgaa ttgggtggca gtgacggtga tctcgccgcc caggcgatcg cggtggaaga gctctcagcc gcatgtgcct cgactgcgat ggtcttgctg gtcaattggg ccggcacctg caccgtcatt catcagggat ccgacgagct caagaagttg gtggtgcccg atgtctccac cggtgctgtg ggagcgggat ggtgcttgac cgaacccacc ggcggatcgg atctctctgg tattcgcacc aaggcgcaac gtgacgggtc cgattgggtt ctcaacggcc agaagcgctt catcagcaac gcggactggg gcgattggtt cgcggtgctg gcccgttccg gtgaggagaa cgagttcggc gtattcatgg tccacaagag cgacgccggc ctcagcttcg ggccacccga gaagaagatg ggacttcgcg gaagccccac ggctgatgtc attctcgaag actgccggat ccccggcgaa cgagtcgtcg gggatcccac ccaaggctac aaatacatca tcgacgagtt gaacggaagt cgggctctga tcgcggcgca ggcactcggt ttggcaaagg gcgcgcttcg cacggcgatc gaatacaccg ccgagcgccg ccagttcgga tcagcgctgt cgacatttca gatggtgcgc gccatggtcg ccgacgcagc gatccgcgtc gagtccgcaa gtgccctgct ctacaaggcc gtcgacgcca tcgagaacaa agatcctcgc gctcgttcgc tggtttccat ggcaaaagtg ctgtgtagtg acaacgccat ggcggtaacg acagacgctg ttcaactcct cggcggttac gggttcacgc gcgagtaccc cgtggagcga atgatgcgag acgcgaaggt gactcagatc tatgagggga gtaatcagat ccagcgtctg gtgatctcga agggactgta caaaggggag gcgctgtcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.00185.a.A1.PS00554
PURIFICATION DATe: 4/8/2010
CONCENTRATION: 7.57mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNTELLDFR AMMQDLASTQ VAPYARDVDE QERFPEEAWQ ALCAADLPGL AHPTELGGSD GDLAAQAIAV EELSAACAST AMVLLVNWAG TCTVIHQGSD ELKKLVVPDV STGAVGAGWC LTEPTGGSDL SGIRTKAQRD GSDWVLNGQK RFISNADWGD WFAVLARSGE ENEFGVFMVH KSDAGLSFGP PEKKMGLRGS PTADVILEDC RIPGERVVGD PTQGYKYIID ELNGSRALIA AQALGLAKGA LRTAIEYTAE RRQFGSALST FQMVRAMVAD AAIRVESASA LLYKAVDAIE NKDPRARSLV SMAKVLCSDN AMAVTTDAVQ LLGGYGFTRE YPVERMMRDA KVTQIYEGSN QIQRLVISKG LYKGEALS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca cagaattgct ggatttccgg gcgatgatgc aagacctggc gtccacccag gtggcaccgt acgcgcgtga cgtcgacgag caggaacggt tcccagaaga ggcgtggcag gcgctctgcg ccgccgacct gcccggactt gctcacccca ctgaattggg tggcagtgac ggtgatctcg ccgcccaggc gatcgcggtg gaagagctct cagccgcatg tgcctcgact gcgatggtct tgctggtcaa ttgggccggc acctgcaccg tcattcatca gggatccgac gagctcaaga agttggtggt gcccgatgtc tccaccggtg ctgtgggagc gggatggtgc ttgaccgaac ccaccggcgg atcggatctc tctggtattc gcaccaaggc gcaacgtgac gggtccgatt gggttctcaa cggccagaag cgcttcatca gcaacgcgga ctggggcgat tggttcgcgg tgctggcccg ttccggtgag gagaacgagt tcggcgtatt catggtccac aagagcgacg ccggcctcag cttcgggcca cccgagaaga agatgggact tcgcggaagc cccacggctg atgtcattct cgaagactgc cggatccccg gcgaacgagt cgtcggggat cccacccaag gctacaaata catcatcgac gagttgaacg gaagtcgggc tctgatcgcg gcgcaggcac tcggtttggc aaagggcgcg cttcgcacgg cgatcgaata caccgccgag cgccgccagt tcggatcagc gctgtcgaca tttcagatgg tgcgcgccat ggtcgccgac gcagcgatcc gcgtcgagtc cgcaagtgcc ctgctctaca aggccgtcga cgccatcgag aacaaagatc ctcgcgctcg ttcgctggtt tccatggcaa aagtgctgtg tagtgacaac gccatggcgg taacgacaga cgctgttcaa ctcctcggcg gttacgggtt cacgcgcgag taccccgtgg agcgaatgat gcgagacgcg aaggtgactc agatctatga ggggagtaat cagatccagc gtctggtgat ctcgaaggga ctgtacaaag gggaggcgct gtcataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTELLDFR AMMQDLASTQ VAPYARDVDE QERFPEEAWQ ALCAADLPGL AHPTELGGSD GDLAAQAIAV EELSAACAST AMVLLVNWAG TCTVIHQGSD ELKKLVVPDV STGAVGAGWC LTEPTGGSDL SGIRTKAQRD GSDWVLNGQK RFISNADWGD WFAVLARSGE ENEFGVFMVH KSDAGLSFGP PEKKMGLRGS PTADVILEDC RIPGERVVGD PTQGYKYIID ELNGSRALIA AQALGLAKGA LRTAIEYTAE RRQFGSALST FQMVRAMVAD AAIRVESASA LLYKAVDAIE NKDPRARSLV SMAKVLCSDN AMAVTTDAVQ LLGGYGFTRE YPVERMMRDA KVTQIYEGSN QIQRLVISKG LYKGEALS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACACAGAA TTGCTGGATT TCCGGGCGAT GATGCAAGAC CTGGCGTCCA CCCAGGTGGC ACCGTACGCG CGTGACGTCG ACGAGCAGGA ACGGTTCCCA GAAGAGGCGT GGCAGGCGCT CTGCGCCGCC GACCTGCCCG GACTTGCTCA CCCCACTGAA TTGGGTGGCA GTGACGGTGA TCTCGCCGCC CAGGCGATCG CGGTGGAAGA GCTCTCAGCC GCATGTGCCT CGACTGCGAT GGTCTTGCTG GTCAATTGGG CCGGCACCTG CACCGTCATT CATCAGGGAT CCGACGAGCT CAAGAAGTTG GTGGTGCCCG ATGTCTCCAC CGGTGCTGTG GGAGCGGGAT GGTGCTTGAC CGAACCCACC GGCGGATCGG ATCTCTCTGG TATTCGCACC AAGGCGCAAC GTGACGGGTC CGATTGGGTT CTCAACGGCC AGAAGCGCTT CATCAGCAAC GCGGACTGGG GCGATTGGTT CGCGGTGCTG GCCCGTTCCG GTGAGGAGAA CGAGTTCGGC GTATTCATGG TCCACAAGAG CGACGCCGGC CTCAGCTTCG GGCCACCCGA GAAGAAGATG GGACTTCGCG GAAGCCCCAC GGCTGATGTC ATTCTCGAAG ACTGCCGGAT CCCCGGCGAA CGAGTCGTCG GGGATCCCAC CCAAGGCTAC AAATACATCA TCGACGAGTT GAACGGAAGT CGGGCTCTGA TCGCGGCGCA GGCACTCGGT TTGGCAAAGG GCGCGCTTCG CACGGCGATC GAATACACCG CCGAGCGCCG CCAGTTCGGA TCAGCGCTGT CGACATTTCA GATGGTGCGC GCCATGGTCG CCGACGCAGC GATCCGCGTC GAGTCCGCAA GTGCCCTGCT CTACAAGGCC GTCGACGCCA TCGAGAACAA AGATCCTCGC GCTCGTTCGC TGGTTTCCAT GGCAAAAGTG CTGTGTAGTG ACAACGCCAT GGCGGTAACG ACAGACGCTG TTCAACTCCT CGGCGGTTAC GGGTTCACGC GCGAGTACCC CGTGGAGCGA ATGATGCGAG ACGCGAAGGT GACTCAGATC TATGAGGGGA GTAATCAGAT CCAGCGTCTG GTGATCTCGA AGGGACTGTA CAAAGGGGAG GCGCTGTCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A