MyabA.00185.b

Probable acyl-CoA dehydrogenase FadE

CENTER ID:	MyabA.00185.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00185.b.A1.GE30262	Full length( MyabA.00185.b )		1	387

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00185.b.A1.PS00880	Full length( MyabA.00185.b )		1	387

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129653
PATRIC ID:	fig\|36809.5.peg.3686
RefSeq:	YP_001704346.1
UniProt:	B1MF86

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGNPSFDLF KLGEEHDELR AAIRGLAEKE IAPHAKDVDE KARFPQEALD ALVASGFNAV HVPEEYDGQG ADSVAACIVI EEVARVCASS SLIPAVNKLG TMGLILNGSD ELKKQVLPAI ASGEAMASYA LSEREAGSDA ASMRTRAKAD GDDWIINGSK CWITNGGKSS WYTVMAVTDP EKKANGISAF MVHKDDEGFT VGPLEHKLGI KGSPTAELYF ENCRIPGDRI IGEPGTGFKT ALQTLDHTRP TIGAQALGIA QGALDQAIAY TKDRKQFGKS ISDFQGVQFM LADMAMKVEA ARLMVYTAAA RAERGEKNLG FISSASKCFA SDVAMEVTTD AVQLFGGAGY TTDFPVERMM RDAKITQIYE GTNQIQRVVM SRALLTS

NT Sequence

ATGGCTGGGA ACCCGTCGTT TGACCTGTTC AAGTTGGGCG AGGAGCACGA CGAGCTGCGT GCGGCGATCC GGGGACTGGC CGAGAAAGAG ATCGCACCGC ACGCCAAGGA CGTCGACGAG AAGGCACGCT TCCCGCAGGA GGCCCTCGAC GCGCTGGTCG CGTCCGGTTT CAATGCCGTG CACGTGCCGG AGGAGTACGA CGGTCAGGGT GCCGACAGCG TGGCCGCCTG CATTGTCATC GAAGAGGTGG CCCGGGTGTG TGCCTCGTCC TCGCTGATCC CTGCCGTCAA CAAGCTCGGC ACCATGGGGC TGATCCTCAA CGGCTCCGAT GAGCTGAAGA AGCAGGTGCT GCCCGCGATC GCCAGCGGCG AGGCGATGGC CTCCTACGCG CTGTCCGAGC GGGAGGCCGG AAGTGATGCC GCGAGCATGC GCACCCGCGC GAAGGCCGAC GGCGATGACT GGATCATCAA CGGGTCCAAG TGCTGGATCA CCAACGGCGG AAAGTCGTCC TGGTACACCG TCATGGCGGT GACCGATCCG GAGAAGAAGG CCAACGGCAT CTCGGCCTTC ATGGTGCACA AGGACGATGA AGGATTCACT GTCGGTCCGC TGGAGCACAA GCTGGGCATC AAGGGCTCGC CGACCGCGGA GCTGTACTTC GAGAACTGTC GTATCCCGGG CGACCGCATC ATCGGCGAAC CGGGCACCGG ATTCAAGACG GCGCTTCAGA CGCTCGACCA CACCCGCCCC ACCATCGGGG CCCAGGCGCT CGGCATCGCG CAGGGAGCGT TGGACCAGGC CATCGCCTAC ACCAAGGACC GCAAGCAGTT CGGCAAGTCC ATCAGCGACT TCCAGGGCGT GCAGTTCATG CTGGCTGACA TGGCGATGAA GGTCGAGGCC GCGCGCCTCA TGGTGTACAC CGCCGCCGCC CGCGCCGAGC GCGGCGAGAA GAACCTGGGC TTCATCTCCT CGGCGTCCAA ATGTTTTGCC AGCGATGTGG CCATGGAGGT CACCACCGAC GCCGTACAGC TGTTCGGCGG CGCCGGATAC ACCACCGATT TTCCGGTGGA GCGCATGATG CGTGATGCCA AGATCACTCA GATCTACGAG GGCACCAACC AGATCCAGCG CGTCGTCATG AGCCGCGCGC TGCTCACGTC C

Details for MyabA.00185.b.A1.GE30262

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	43kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattaggac gtgagcagcg cgcggctcat gacgacgcgc tggatctggt tggtgccctc gtagatctga gtgatcttgg catcacgcat catgcgctcc accggaaaat cggtggtgta tccggcgccg ccgaacagct gtacggcgtc ggtggtgacc tccatggcca catcgctggc aaaacatttg gacgccgagg agatgaagcc caggttcttc tcgccgcgct cggcgcgggc ggcggcggtg tacaccatga ggcgcgcggc ctcgaccttc atcgccatgt cagccagcat gaactgcacg ccctggaagt cgctgatgga cttgccgaac tgcttgcggt ccttggtgta ggcgatggcc tggtccaacg ctccctgcgc gatgccgagc gcctgggccc cgatggtggg gcgggtgtgg tcgagcgtct gaagcgccgt cttgaatccg gtgcccggtt cgccgatgat gcggtcgccc gggatacgac agttctcgaa gtacagctcc gcggtcggcg agcccttgat gcccagcttg tgctccagcg gaccgacagt gaatccttca tcgtccttgt gcaccatgaa ggccgagatg ccgttggcct tcttctccgg atcggtcacc gccatgacgg tgtaccagga cgactttccg ccgttggtga tccagcactt ggacccgttg atgatccagt catcgccgtc ggccttcgcg cgggtgcgca tgctcgcggc atcacttccg gcctcccgct cggacagcgc gtaggaggcc atcgcctcgc cgctggcgat cgcgggcagc acctgcttct tcagctcatc ggagccgttg aggatcagcc ccatggtgcc gagcttgttg acggcaggga tcagcgagga cgaggcacac acccgggcca cctcttcgat gacaatgcag gcggccacgc tgtcggcacc ctgaccgtcg tactcctccg gcacgtgcac ggcattgaaa ccggacgcga ccagcgcgtc gagggcctcc tgcgggaagc gtgccttctc gtcgacgtcc ttggcgtgcg gtgcgatctc tttctcggcc agtccccgga tcgccgcacg cagctcgtcg tgctcctcgc ccaacttgaa caggtcaaac gacgggttcc cagccatcga accaggaccc tgggtctgag cttccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGNPSFDL FKLGEEHDEL RAAIRGLAEK EIAPHAKDVD EKARFPQEAL DALVASGFNA VHVPEEYDGQ GADSVAACIV IEEVARVCAS SSLIPAVNKL GTMGLILNGS DELKKQVLPA IASGEAMASY ALSEREAGSD AASMRTRAKA DGDDWIINGS KCWITNGGKS SWYTVMAVTD PEKKANGISA FMVHKDDEGF TVGPLEHKLG IKGSPTAELY FENCRIPGDR IIGEPGTGFK TALQTLDHTR PTIGAQALGI AQGALDQAIA YTKDRKQFGK SISDFQGVQF MLADMAMKVE AARLMVYTAA ARAERGEKNL GFISSASKCF ASDVAMEVTT DAVQLFGGAG YTTDFPVERM MRDAKITQIY EGTNQIQRVV MSRALLTS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgggaac ccgtcgtttg acctgttcaa gttgggcgag gagcacgacg agctgcgtgc ggcgatccgg ggactggccg agaaagagat cgcaccgcac gccaaggacg tcgacgagaa ggcacgcttc ccgcaggagg ccctcgacgc gctggtcgcg tccggtttca atgccgtgca cgtgccggag gagtacgacg gtcagggtgc cgacagcgtg gccgcctgca ttgtcatcga agaggtggcc cgggtgtgtg cctcgtcctc gctgatccct gccgtcaaca agctcggcac catggggctg atcctcaacg gctccgatga gctgaagaag caggtgctgc ccgcgatcgc cagcggcgag gcgatggcct cctacgcgct gtccgagcgg gaggccggaa gtgatgccgc gagcatgcgc acccgcgcga aggccgacgg cgatgactgg atcatcaacg ggtccaagtg ctggatcacc aacggcggaa agtcgtcctg gtacaccgtc atggcggtga ccgatccgga gaagaaggcc aacggcatct cggccttcat ggtgcacaag gacgatgaag gattcactgt cggtccgctg gagcacaagc tgggcatcaa gggctcgccg accgcggagc tgtacttcga gaactgtcgt atcccgggcg accgcatcat cggcgaaccg ggcaccggat tcaagacggc gcttcagacg ctcgaccaca cccgccccac catcggggcc caggcgctcg gcatcgcgca gggagcgttg gaccaggcca tcgcctacac caaggaccgc aagcagttcg gcaagtccat cagcgacttc cagggcgtgc agttcatgct ggctgacatg gcgatgaagg tcgaggccgc gcgcctcatg gtgtacaccg ccgccgcccg cgccgagcgc ggcgagaaga acctgggctt catctcctcg gcgtccaaat gttttgccag cgatgtggcc atggaggtca ccaccgacgc cgtacagctg ttcggcggcg ccggatacac caccgatttt ccggtggagc gcatgatgcg tgatgccaag atcactcaga tctacgaggg caccaaccag atccagcgcg tcgtcatgag ccgcgcgctg ctcacgtcca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MyabA.00185.b.A1.PS00880

PURIFICATION DATe:	11/24/2010
CONCENTRATION:	52.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGGGAAC CCGTCGTTTG ACCTGTTCAA GTTGGGCGAG GAGCACGACG AGCTGCGTGC GGCGATCCGG GGACTGGCCG AGAAAGAGAT CGCACCGCAC GCCAAGGACG TCGACGAGAA GGCACGCTTC CCGCAGGAGG CCCTCGACGC GCTGGTCGCG TCCGGTTTCA ATGCCGTGCA CGTGCCGGAG GAGTACGACG GTCAGGGTGC CGACAGCGTG GCCGCCTGCA TTGTCATCGA AGAGGTGGCC CGGGTGTGTG CCTCGTCCTC GCTGATCCCT GCCGTCAACA AGCTCGGCAC CATGGGGCTG ATCCTCAACG GCTCCGATGA GCTGAAGAAG CAGGTGCTGC CCGCGATCGC CAGCGGCGAG GCGATGGCCT CCTACGCGCT GTCCGAGCGG GAGGCCGGAA GTGATGCCGC GAGCATGCGC ACCCGCGCGA AGGCCGACGG CGATGACTGG ATCATCAACG GGTCCAAGTG CTGGATCACC AACGGCGGAA AGTCGTCCTG GTACACCGTC ATGGCGGTGA CCGATCCGGA GAAGAAGGCC AACGGCATCT CGGCCTTCAT GGTGCACAAG GACGATGAAG GATTCACTGT CGGTCCGCTG GAGCACAAGC TGGGCATCAA GGGCTCGCCG ACCGCGGAGC TGTACTTCGA GAACTGTCGT ATCCCGGGCG ACCGCATCAT CGGCGAACCG GGCACCGGAT TCAAGACGGC GCTTCAGACG CTCGACCACA CCCGCCCCAC CATCGGGGCC CAGGCGCTCG GCATCGCGCA GGGAGCGTTG GACCAGGCCA TCGCCTACAC CAAGGACCGC AAGCAGTTCG GCAAGTCCAT CAGCGACTTC CAGGGCGTGC AGTTCATGCT GGCTGACATG GCGATGAAGG TCGAGGCCGC GCGCCTCATG GTGTACACCG CCGCCGCCCG CGCCGAGCGC GGCGAGAAGA ACCTGGGCTT CATCTCCTCG GCGTCCAAAT GTTTTGCCAG CGATGTGGCC ATGGAGGTCA CCACCGACGC CGTACAGCTG TTCGGCGGCG CCGGATACAC CACCGATTTT CCGGTGGAGC GCATGATGCG TGATGCCAAG ATCACTCAGA TCTACGAGGG CACCAACCAG ATCCAGCGCG TCGTCATGAG CCGCGCGCTG CTCACGTCCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A