MythA.01263.b

Acyl-CoA dehydrogenase fadE12 (A0R3J4 homolog)

CENTER ID: MythA.01263.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01263.b.A1.GE28775 Full length( MythA.01263.b ) 1 799
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01263.b.A1.PW28677 Full length( MythA.01263.b ) 1 799

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.476
RefSeq: WP_003923909.1
UniProt: G7CBZ0

Orthologues for Rv0972c

Species % Identity % Coverage Clones Proteins Structures
marinum 91 100 1 1 0
ulcerans 91 100 0 0 0
avium 90 100 0 1 0
paratuberculosis 90 100 1 1 0
smegmatis 82 99 1 2 0
thermoresistibile 52 98 1 1 0
thermoresistibile 37 99 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSADTMSDTR VGTAQRLYEA LARGDRDALD ELLHPDFVGR AAEGLPLQMG GEHVGAEAMR TNLWWRIGEH FKVRALAEDF QILPDGRLMV GGRYRGSARR SGKPLDAAFI HLIAFGDDGR VVALEQLTDT AAWHEALDAP AELETIDYRV VNGVATVCLN RPEMRNAIDM RMGRETLEVA RRIEGDHTVR CVLICGNGPA LTVGGDISYF LDGRSERFGD LFAEMTTPFH QAFDILSRVP VPIVTAAHGA VAGGGLGYVY AAXXXXXXXX XXXXXXXXXX XGHRVVRRRR RHLASTPADR SAPRRGGVSA EPADRGGAGA GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXPWPSAPC GYCCATLGAT IFRRNSPRRR AVSNSPATAK TPPPRSTRSP PSGRPCSSEG RDMSGLLHET DEHRAIRETV AGIAQRYGVH YYLERGRAGG NIDELWKDLG AAGLLGVHLP EEYGGGGGGM AEAVIVVEEL AAHGMPLLIW VISPAICGSI LAHHGSDAMR KDWLPTIADG SRRMAFGLTE PDAGSNSHNV KTTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXS TPEKSRLSLF VVPTDAPGLT YQQIPTSIVS PDKQFTVFLD NVEVGEEALI GEAGNGLRQV FDGLNPERIM VGALCGGIGR YAIGKAAEYA KERTVWSTPI GAHQGVAHPL AECHIAVELS RLVTARSAEL FDAGEPAGEA ANIAKFAASE AALKALDQAI QTHGGNGLSY EYGLSELWFV TRLMRTAPVS REMVLNFVAQ TSLGLPRSY
NT Sequence
ATGTCGGCTG ACACCATGTC CGACACCAGA GTCGGCACCG CGCAGCGGCT CTACGAGGCA CTGGCACGCG GAGACCGGGA CGCACTGGAT GAACTGCTGC ATCCGGACTT CGTCGGCCGC GCGGCCGAGG GGCTGCCGCT GCAGATGGGC GGCGAGCACG TCGGCGCGGA GGCGATGCGC ACCAACCTGT GGTGGCGCAT CGGCGAGCAC TTCAAGGTCC GCGCACTGGC CGAGGACTTC CAGATCCTGC CGGACGGCCG GCTGATGGTC GGGGGGCGGT ACCGGGGTTC GGCCAGGCGC AGCGGAAAAC CGTTGGACGC CGCCTTCATC CACCTCATCG CCTTCGGTGA CGACGGTCGC GTCGTTGCCC TGGAGCAGTT GACCGACACC GCGGCCTGGC ACGAGGCTCT CGACGCGCCG GCGGAGCTGG AGACCATTGA CTACCGCGTC GTCAACGGCG TTGCGACGGT GTGCCTGAAC CGGCCAGAGA TGCGCAACGC CATCGACATG CGCATGGGCC GGGAGACGCT CGAGGTGGCC CGGCGCATCG AGGGCGACCA TACCGTGCGG TGCGTGCTCA TCTGCGGCAA CGGGCCGGCG CTCACCGTGG GCGGCGACAT CAGCTACTTC CTGGACGGCC GCTCCGAACG GTTCGGTGAT CTGTTCGCCG AGATGACCAC CCCGTTCCAC CAGGCCTTCG ACATCCTCAG CCGGGTTCCG GTCCCGATCG TCACCGCCGC GCACGGTGCG GTGGCCGGCG GCGGGCTCGG CTACGTCTAC GCGGCNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCGGGCATC GGGTTGTCCG GCGACGGCGG CGGCACCTGG CATCTACCCC GGCTGATCGG TCCGCGCCGC GCCGCGGAGG CGTATCTGCG GAACCAGCCG ATCGAGGCGG AGCAGGCGCT GGANNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNAC CCTGGCCTTC GGCACCATGC GGGTACTGCT GCGCGACTCT TGGCGCAACG ATCTTTCGGC GCAACTCGCC GCGGAGACGC GCGGTATCAA ACTCACCGGC GACAGCGAAG ACGCCGCCGC CGCGATCAAC GCGTTCGCCG CCAAGCGGAC GCCCATGTTC ATCGGAAGGT AGAGACATGT CGGGGTTGTT GCACGAGACT GACGAGCACC GAGCGATCCG GGAGACCGTC GCCGGGATCG CACAGCGTTA CGGTGTTCAC TACTACCTCG AACGCGGCCG TGCCGGCGGG AACATCGACG AGCTCTGGAA GGATCTCGGC GCGGCCGGGC TACTCGGCGT GCACCTCCCG GAGGAGTACG GCGGTGGCGG CGGTGGCATG GCCGAAGCGG TCATCGTGGT GGAAGAGCTT GCCGCCCACG GGATGCCGCT GCTGATCTGG GTGATCTCAC CGGCCATCTG CGGTAGCATC CTGGCCCACC ACGGGTCGGA CGCCATGCGG AAGGACTGGC TTCCGACCAT CGCCGACGGC TCGCGCAGGA TGGCCTTCGG TCTGACCGAA CCGGACGCCG GATCCAACAG CCACAACGTC AAGACGACNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGTCG ACACCGGAGA AGTCGAGACT GTCGCTGTTC GTGGTGCCGA CCGATGCGCC GGGGCTGACC TATCAGCAGA TTCCGACGTC GATCGTGTCG CCCGACAAGC AGTTCACGGT GTTCCTCGAC AACGTCGAGG TCGGTGAGGA GGCCCTGATC GGCGAGGCGG GCAACGGCCT GCGCCAGGTG TTCGACGGCC TGAACCCGGA ACGGATCATG GTGGGCGCAC TGTGCGGCGG CATCGGTCGA TACGCCATCG GGAAGGCCGC CGAGTACGCC AAGGAGCGTA CCGTGTGGTC GACGCCGATC GGCGCCCACC AGGGGGTGGC CCACCCGCTG GCCGAATGCC ACATCGCGGT GGAGCTGAGC CGGTTGGTGA CGGCACGCAG CGCCGAACTG TTCGACGCCG GGGAACCCGC CGGCGAGGCG GCCAACATCG CCAAGTTCGC CGCCTCGGAA GCCGCGCTGA AAGCGCTCGA CCAGGCAATC CAGACCCACG GCGGCAACGG ACTGTCCTAC GAGTACGGCC TGTCGGAACT GTGGTTCGTC ACCCGCCTCA TGCGCACCGC GCCGGTCAGC CGGGAGATGG TGCTGAACTT CGTGGCGCAG ACCTCGCTGG GTCTGCCCCG GTCCTAC
Details for MythA.01263.b.A1.GE28775
HARVESTED ON: data unavailable
SEQUENCED ON: 3/5/2010
EXPECTED MW: 89kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 31
Validated AA Sequence
MSADTMSDTR VGTAQRLYEA LARGDRDALD ELLHPDFVGR AAEGLPLQMG GEHVGAEAMR TNLWWRIGEH FKVRALAEDF QILPDGRLMV GGRYRGSARR SGKPLDAAFI HLIAFGDDGR VVALEQLTDT AAWHEALDAP AELETIDYRV VNGVATVCLN RPEMRNAIDM RMGRETLEVA RRIEGDHTVR CVLICGNGPA LTVGGDISYF LDGRSERFGX LFAEMTTPFH QXXDILSRVP VPIVTAAHGA XXGGGXG
Validated NT Sequence
atcaccatca ccntnnnntg ggtaccctgg aagctcagac ccagggtcct ggttcgatgt cggctgacac catgtccgac accagagtcg gcaccgcgca gcggctctac gaggcactgg cacgcggaga ccgggacgca ctggatgaac tgctgcatcc ggacttcgtc ggccgcgcgg ccgaggggct gccgctgcag atgggcggcg agcacgtcgg cgcggaggcg atgcgcacca acctgtggtg gcgcatcggc gagcacttca aggtccgcgc actggccgag gacttccaga tcctgccgga cggccggctg atggtcgggg ggcggtaccg gggttcggcc aggcgcagcg gaaaaccgtt ggacgccgcc ttcatccacc tcatcgcctt cggtgacgac ggtcgcgtcg ttgccctgga gcagttgacc gacaccgcgg cctggcacga ggctctcgac gcgccggcgg agctggagac cattgactac cgcgtcgtca acggcgttgc gacggtgtgc ctgaaccggc cagagatgcg caacgccatc gacatgcgca tgggccggga gacgctcgag gtggcccggc gcatcgaggg cgaccatacc gtgcggtgcg tgctcatctg cggcaacggg ccggcgctca ccgtgggcgg cgacatcagc tacttcctgg acggccgctc cgaacggttc ggtganctgt tcgccgagat gaccaccccg ttccaccagg ncntcgacat cctcagccgg gttccggtcc cgatcgtcac cgccgcgcac ggtgcggngg ncggnggcgg gntcggntac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSADTMSDT RVGTAQRLYE ALARGDRDAL DELLHPDFVG RAAEGLPLQM GGEHVGAEAM RTNLWWRIGE HFKVRALAED FQILPDGRLM VGGRYRGSAR RSGKPLDAAF IHLIAFGDDG RVVALEQLTD TAAWHEALDA PAELETIDYR VVNGVATVCL NRPEMRNAID MRMGRETLEV ARRIEGDHTV RCVLICGNGP ALTVGGDISY FLDGRSERFG DLFAEMTTPF HQAFDILSRV PVPIVTAAHG AVAGGGLGYV YAXXXXXXXX XXXXXXXXXX XXGHRVVRRR RRHLASTPAD RSAPRRGGVS AEPADRGGAG AGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPWPSAP CGYCCATLGA TIFRRNSPRR RAVSNSPATA KTPPPRSTRS PPSGRPCSSE GRDMSGLLHE TDEHRAIRET VAGIAQRYGV HYYLERGRAG GNIDELWKDL GAAGLLGVHL PEEYGGGGGG MAEAVIVVEE LAAHGMPLLI WVISPAICGS ILAHHGSDAM RKDWLPTIAD GSRRMAFGLT EPDAGSNSHN VKTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX STPEKSRLSL FVVPTDAPGL TYQQIPTSIV SPDKQFTVFL DNVEVGEEAL IGEAGNGLRQ VFDGLNPERI MVGALCGGIG RYAIGKAAEY AKERTVWSTP IGAHQGVAHP LAECHIAVEL SRLVTARSAE LFDAGEPAGE AANIAKFAAS EAALKALDQA IQTHGGNGLS YEYGLSELWF VTRLMRTAPV SREMVLNFVA QTSLGLPRSY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcggctgac accatgtccg acaccagagt cggcaccgcg cagcggctct acgaggcact ggcacgcgga gaccgggacg cactggatga actgctgcat ccggacttcg tcggccgcgc ggccgagggg ctgccgctgc agatgggcgg cgagcacgtc ggcgcggagg cgatgcgcac caacctgtgg tggcgcatcg gcgagcactt caaggtccgc gcactggccg aggacttcca gatcctgccg gacggccggc tgatggtcgg ggggcggtac cggggttcgg ccaggcgcag cggaaaaccg ttggacgccg ccttcatcca cctcatcgcc ttcggtgacg acggtcgcgt cgttgccctg gagcagttga ccgacaccgc ggcctggcac gaggctctcg acgcgccggc ggagctggag accattgact accgcgtcgt caacggcgtt gcgacggtgt gcctgaaccg gccagagatg cgcaacgcca tcgacatgcg catgggccgg gagacgctcg aggtggcccg gcgcatcgag ggcgaccata ccgtgcggtg cgtgctcatc tgcggcaacg ggccggcgct caccgtgggc ggcgacatca gctacttcct ggacggccgc tccgaacggt tcggtgatct gttcgccgag atgaccaccc cgttccacca ggccttcgac atcctcagcc gggttccggt cccgatcgtc accgccgcgc acggtgcggt ggccggcggc gggctcggct acgtctacgc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgggcatcgg gttgtccggc gacggcggcg gcacctggca tctaccccgg ctgatcggtc cgcgccgcgc cgcggaggcg tatctgcgga accagccgat cgaggcggag caggcgctgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc tggccttcgg caccatgcgg gtactgctgc gcgactcttg gcgcaacgat ctttcggcgc aactcgccgc ggagacgcgc ggtatcaaac tcaccggcga cagcgaagac gccgccgccg cgatcaacgc gttcgccgcc aagcggacgc ccatgttcat cggaaggtag agacatgtcg gggttgttgc acgagactga cgagcaccga gcgatccggg agaccgtcgc cgggatcgca cagcgttacg gtgttcacta ctacctcgaa cgcggccgtg ccggcgggaa catcgacgag ctctggaagg atctcggcgc ggccgggcta ctcggcgtgc acctcccgga ggagtacggc ggtggcggcg gtggcatggc cgaagcggtc atcgtggtgg aagagcttgc cgcccacggg atgccgctgc tgatctgggt gatctcaccg gccatctgcg gtagcatcct ggcccaccac gggtcggacg ccatgcggaa ggactggctt ccgaccatcg ccgacggctc gcgcaggatg gccttcggtc tgaccgaacc ggacgccgga tccaacagcc acaacgtcaa gacgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcgac accggagaag tcgagactgt cgctgttcgt ggtgccgacc gatgcgccgg ggctgaccta tcagcagatt ccgacgtcga tcgtgtcgcc cgacaagcag ttcacggtgt tcctcgacaa cgtcgaggtc ggtgaggagg ccctgatcgg cgaggcgggc aacggcctgc gccaggtgtt cgacggcctg aacccggaac ggatcatggt gggcgcactg tgcggcggca tcggtcgata cgccatcggg aaggccgccg agtacgccaa ggagcgtacc gtgtggtcga cgccgatcgg cgcccaccag ggggtggccc acccgctggc cgaatgccac atcgcggtgg agctgagccg gttggtgacg gcacgcagcg ccgaactgtt cgacgccggg gaacccgccg gcgaggcggc caacatcgcc aagttcgccg cctcggaagc cgcgctgaaa gcgctcgacc aggcaatcca gacccacggc ggcaacggac tgtcctacga gtacggcctg tcggaactgt ggttcgtcac ccgcctcatg cgcaccgcgc cggtcagccg ggagatggtg ctgaacttcg tggcgcagac ctcgctgggt ctgccccggt cctacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MythA.01263.b.A1.PW28677
PURIFICATION DATe: 6/21/2010
CONCENTRATION: 23.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 31
Protocol Notes
notes unavailable
Validated AA Sequence
MSADTMSDTR VGTAQRLYEA LARGDRDALD ELLHPDFVGR AAEGLPLQMG GEHVGAEAMR TNLWWRIGEH FKVRALAEDF QILPDGRLMV GGRYRGSARR SGKPLDAAFI HLIAFGDDGR VVALEQLTDT AAWHEALDAP AELETIDYRV VNGVATVCLN RPEMRNAIDM RMGRETLEVA RRIEGDHTVR CVLICGNGPA LTVGGDISYF LDGRSERFGX LFAEMTTPFH QXXDILSRVP VPIVTAAHGA XXGGGXG
Validated NT Sequence
atcaccatca ccntnnnntg ggtaccctgg aagctcagac ccagggtcct ggttcgatgt cggctgacac catgtccgac accagagtcg gcaccgcgca gcggctctac gaggcactgg cacgcggaga ccgggacgca ctggatgaac tgctgcatcc ggacttcgtc ggccgcgcgg ccgaggggct gccgctgcag atgggcggcg agcacgtcgg cgcggaggcg atgcgcacca acctgtggtg gcgcatcggc gagcacttca aggtccgcgc actggccgag gacttccaga tcctgccgga cggccggctg atggtcgggg ggcggtaccg gggttcggcc aggcgcagcg gaaaaccgtt ggacgccgcc ttcatccacc tcatcgcctt cggtgacgac ggtcgcgtcg ttgccctgga gcagttgacc gacaccgcgg cctggcacga ggctctcgac gcgccggcgg agctggagac cattgactac cgcgtcgtca acggcgttgc gacggtgtgc ctgaaccggc cagagatgcg caacgccatc gacatgcgca tgggccggga gacgctcgag gtggcccggc gcatcgaggg cgaccatacc gtgcggtgcg tgctcatctg cggcaacggg ccggcgctca ccgtgggcgg cgacatcagc tacttcctgg acggccgctc cgaacggttc ggtganctgt tcgccgagat gaccaccccg ttccaccagg ncntcgacat cctcagccgg gttccggtcc cgatcgtcac cgccgcgcac ggtgcggngg ncggnggcgg gntcggntac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSADTMSDT RVGTAQRLYE ALARGDRDAL DELLHPDFVG RAAEGLPLQM GGEHVGAEAM RTNLWWRIGE HFKVRALAED FQILPDGRLM VGGRYRGSAR RSGKPLDAAF IHLIAFGDDG RVVALEQLTD TAAWHEALDA PAELETIDYR VVNGVATVCL NRPEMRNAID MRMGRETLEV ARRIEGDHTV RCVLICGNGP ALTVGGDISY FLDGRSERFG DLFAEMTTPF HQAFDILSRV PVPIVTAAHG AVAGGGLGYV YAXXXXXXXX XXXXXXXXXX XXGHRVVRRR RRHLASTPAD RSAPRRGGVS AEPADRGGAG AGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPWPSAP CGYCCATLGA TIFRRNSPRR RAVSNSPATA KTPPPRSTRS PPSGRPCSSE GRDMSGLLHE TDEHRAIRET VAGIAQRYGV HYYLERGRAG GNIDELWKDL GAAGLLGVHL PEEYGGGGGG MAEAVIVVEE LAAHGMPLLI WVISPAICGS ILAHHGSDAM RKDWLPTIAD GSRRMAFGLT EPDAGSNSHN VKTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX STPEKSRLSL FVVPTDAPGL TYQQIPTSIV SPDKQFTVFL DNVEVGEEAL IGEAGNGLRQ VFDGLNPERI MVGALCGGIG RYAIGKAAEY AKERTVWSTP IGAHQGVAHP LAECHIAVEL SRLVTARSAE LFDAGEPAGE AANIAKFAAS EAALKALDQA IQTHGGNGLS YEYGLSELWF VTRLMRTAPV SREMVLNFVA QTSLGLPRSY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGGCTGAC ACCATGTCCG ACACCAGAGT CGGCACCGCG CAGCGGCTCT ACGAGGCACT GGCACGCGGA GACCGGGACG CACTGGATGA ACTGCTGCAT CCGGACTTCG TCGGCCGCGC GGCCGAGGGG CTGCCGCTGC AGATGGGCGG CGAGCACGTC GGCGCGGAGG CGATGCGCAC CAACCTGTGG TGGCGCATCG GCGAGCACTT CAAGGTCCGC GCACTGGCCG AGGACTTCCA GATCCTGCCG GACGGCCGGC TGATGGTCGG GGGGCGGTAC CGGGGTTCGG CCAGGCGCAG CGGAAAACCG TTGGACGCCG CCTTCATCCA CCTCATCGCC TTCGGTGACG ACGGTCGCGT CGTTGCCCTG GAGCAGTTGA CCGACACCGC GGCCTGGCAC GAGGCTCTCG ACGCGCCGGC GGAGCTGGAG ACCATTGACT ACCGCGTCGT CAACGGCGTT GCGACGGTGT GCCTGAACCG GCCAGAGATG CGCAACGCCA TCGACATGCG CATGGGCCGG GAGACGCTCG AGGTGGCCCG GCGCATCGAG GGCGACCATA CCGTGCGGTG CGTGCTCATC TGCGGCAACG GGCCGGCGCT CACCGTGGGC GGCGACATCA GCTACTTCCT GGACGGCCGC TCCGAACGGT TCGGTGATCT GTTCGCCGAG ATGACCACCC CGTTCCACCA GGCCTTCGAC ATCCTCAGCC GGGTTCCGGT CCCGATCGTC ACCGCCGCGC ACGGTGCGGT GGCCGGCGGC GGGCTCGGCT ACGTCTACGC GGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CGGGCATCGG GTTGTCCGGC GACGGCGGCG GCACCTGGCA TCTACCCCGG CTGATCGGTC CGCGCCGCGC CGCGGAGGCG TATCTGCGGA ACCAGCCGAT CGAGGCGGAG CAGGCGCTGG ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNACCC TGGCCTTCGG CACCATGCGG GTACTGCTGC GCGACTCTTG GCGCAACGAT CTTTCGGCGC AACTCGCCGC GGAGACGCGC GGTATCAAAC TCACCGGCGA CAGCGAAGAC GCCGCCGCCG CGATCAACGC GTTCGCCGCC AAGCGGACGC CCATGTTCAT CGGAAGGTAG AGACATGTCG GGGTTGTTGC ACGAGACTGA CGAGCACCGA GCGATCCGGG AGACCGTCGC CGGGATCGCA CAGCGTTACG GTGTTCACTA CTACCTCGAA CGCGGCCGTG CCGGCGGGAA CATCGACGAG CTCTGGAAGG ATCTCGGCGC GGCCGGGCTA CTCGGCGTGC ACCTCCCGGA GGAGTACGGC GGTGGCGGCG GTGGCATGGC CGAAGCGGTC ATCGTGGTGG AAGAGCTTGC CGCCCACGGG ATGCCGCTGC TGATCTGGGT GATCTCACCG GCCATCTGCG GTAGCATCCT GGCCCACCAC GGGTCGGACG CCATGCGGAA GGACTGGCTT CCGACCATCG CCGACGGCTC GCGCAGGATG GCCTTCGGTC TGACCGAACC GGACGCCGGA TCCAACAGCC ACAACGTCAA GACGACNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTCGAC ACCGGAGAAG TCGAGACTGT CGCTGTTCGT GGTGCCGACC GATGCGCCGG GGCTGACCTA TCAGCAGATT CCGACGTCGA TCGTGTCGCC CGACAAGCAG TTCACGGTGT TCCTCGACAA CGTCGAGGTC GGTGAGGAGG CCCTGATCGG CGAGGCGGGC AACGGCCTGC GCCAGGTGTT CGACGGCCTG AACCCGGAAC GGATCATGGT GGGCGCACTG TGCGGCGGCA TCGGTCGATA CGCCATCGGG AAGGCCGCCG AGTACGCCAA GGAGCGTACC GTGTGGTCGA CGCCGATCGG CGCCCACCAG GGGGTGGCCC ACCCGCTGGC CGAATGCCAC ATCGCGGTGG AGCTGAGCCG GTTGGTGACG GCACGCAGCG CCGAACTGTT CGACGCCGGG GAACCCGCCG GCGAGGCGGC CAACATCGCC AAGTTCGCCG CCTCGGAAGC CGCGCTGAAA GCGCTCGACC AGGCAATCCA GACCCACGGC GGCAACGGAC TGTCCTACGA GTACGGCCTG TCGGAACTGT GGTTCGTCAC CCGCCTCATG CGCACCGCGC CGGTCAGCCG GGAGATGGTG CTGAACTTCG TGGCGCAGAC CTCGCTGGGT CTGCCCCGGT CCTACAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA