MyavA.01263.a

Acyl-CoA dehydrogenase fadE12

CENTER ID: MyavA.01263.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01263.a.A1.PW28990 Full length( MyavA.01263.a ) 1 388

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1061
RefSeq: YP_880341.1
UniProt: A0A0H2ZTA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDSSFIENE ERQALRKAVA EWASNYGSEY YLRKARAHEH TNELWSEAGK LGFLGVNLPE EYGGGGAGMY ELSLVMEEMA AAGSALLLMV VSPAINGTII SKFGTEEQKK RWLPGIADGT LTMAFAITEP DAGSNSHKIT TTARRDGSDW ILKGQKVFIS GIDQAQAVLV VARTEEAKTG KLRPALFVVP TDAPGFSATP IEMELISPER QFQVFLDDVR LPSDALVGAE DAAIAQLFAG LNPERIMGAA SSVGMGRFAL ARAAEYVKTR QVWGTPVGAH QGLSHPLAQC HIEVELAKLM MQKAATLYDA GNDMGAAEAA NMAKYAAAEA ATRSVDQAVQ SMGGNGLTKE YGVAAMLASA RLGRIAPVSR EMVLNFVAQT SLGLPRSY
NT Sequence
GTGACCGACA GCAGCTTCAT CGAAAACGAA GAGCGTCAGG CGTTGCGCAA GGCGGTAGCC GAGTGGGCAT CCAACTACGG CAGCGAGTAC TACCTGCGGA AAGCCCGAGC CCACGAGCAC ACCAACGAAT TGTGGTCCGA GGCAGGCAAA CTCGGCTTTC TCGGGGTGAA CCTGCCCGAG GAGTACGGCG GCGGGGGCGC CGGCATGTAC GAGCTGTCGC TGGTGATGGA GGAGATGGCG GCCGCGGGCA GCGCGCTGCT GTTGATGGTG GTGTCGCCGG CCATCAACGG CACCATCATC AGCAAGTTCG GCACCGAGGA GCAGAAGAAG CGCTGGCTTC CCGGGATCGC CGACGGCACG CTGACAATGG CGTTCGCCAT CACCGAACCC GACGCCGGGT CCAACTCGCA CAAGATCACC ACCACCGCCC GCCGCGACGG CAGCGACTGG ATCCTCAAGG GCCAGAAGGT TTTCATTTCC GGCATCGACC AGGCGCAGGC GGTGCTGGTG GTGGCCCGCA CCGAGGAGGC AAAGACCGGC AAGCTGCGGC CCGCGCTGTT CGTGGTGCCC ACCGATGCGC CCGGTTTCAG CGCCACGCCG ATCGAGATGG AATTGATCAG CCCGGAACGC CAGTTCCAGG TCTTCCTCGA CGACGTGCGG CTGCCGTCGG ATGCGCTCGT CGGCGCCGAA GACGCCGCGA TCGCACAGCT TTTCGCCGGG CTCAACCCCG AACGCATCAT GGGCGCGGCC AGCTCCGTCG GGATGGGCCG GTTCGCGCTG GCCCGGGCCG CCGAATACGT CAAGACCCGC CAGGTGTGGG GCACCCCGGT CGGCGCGCAC CAGGGCCTGT CACATCCGTT GGCGCAGTGC CACATCGAGG TCGAACTCGC CAAGCTGATG ATGCAGAAGG CGGCCACCCT GTATGACGCC GGCAACGACA TGGGCGCGGC CGAGGCCGCC AACATGGCCA AATATGCTGC GGCCGAGGCG GCTACCCGCT CGGTCGACCA GGCGGTGCAG TCGATGGGCG GCAACGGCCT CACCAAGGAG TACGGTGTGG CCGCCATGCT GGCCTCGGCC CGGCTGGGCC GCATCGCCCC GGTCAGCCGC GAGATGGTGC TCAACTTCGT CGCCCAGACC TCGCTGGGCC TGCCCCGCAG CTAC
Details for MyavA.01263.a.A1.PW28990
PURIFICATION DATe: 10/18/2010
CONCENTRATION: 28.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 16
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MRPSRAEASM AATPYSLVRP LPPIDCTAWS TERVAASAAA YLAMLAASAA PMSLPASYRV AAFCIISLAS STSMWHCANG CDRPWCAPTG VPHTWRVLTY SAARASANRP IPTELAAPMM RSGLSPAKSC AIAASSAPTS ASDGSRTSSR KTWNWRSGLI NSISIGVALK PGASVGTTNS AGRSLPVFAS SVRATTSTAC AWSMPEMKTF WPLRIQSPPS RRAVVVILCE LDPASGSVMA NAIVSVPSAI PGSQRFFCSS XPNLLMMVPL XAGDXTINRS ALPAXASXXS XRQLVHXGXP XXXXXXXPXK XXXXXXXXXX XXXXXXXXXX LXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttcggncttt gtnagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattagta gctgcggggc aggcccagcg aggtctgggc gacgaagttg agcaccatct cgcggctgac cggggcgatg cggcccagcc gggccgaggc cagcatggcg gccacaccgt actccttggt gaggccgttg ccgcccatcg actgcaccgc ctggtcgacc gagcgggtag ccgcctcggc cgcagcatat ttggccatgt tggcggcctc ggccgcgccc atgtcgttgc cggcgtcata cagggtggcc gccttctgca tcatcagctt ggcgagttcg acctcgatgt ggcactgcgc caacggatgt gacaggccct ggtgcgcgcc gaccggggtg ccccacacct ggcgggtctt gacgtattcg gctgcccggg ccagcgcgaa ccggcccatc ccgacggagc tggccgcgcc catgatgcgt tcggggttga gcccggcgaa aagctgtgcg atcgcggcgt cttcggcgcc gacgagcgca tccgacggca gccgcacgtc gtcgaggaag acctggaact ggcgttcggg gctgatcaat tccatctcga tcggcgtggc gctgaaaccg ggcgcatcgg tgggcaccac gaacagcgcg ggccgcagct tgccggtctt tgcctcctcg gtgcgggcca ccaccagcac cgcctgcgcc tggtcgatgc cggaaatgaa aaccttctgg cccttgagga tccagtcgcc gccgtcgcgg cgggcggtgg tggtgatctt gtgcgagttg gacccggcgt cgggttcggt gatggcgaac gccattgtca gcgtgccgtc ggcgatcccg ggaagccagc gcttcttctg ctcctcggng ccgaacttgc tgatgatggt gccgttgang gccggcgacn ccaccatcaa caggagcgcg ctgcccgcgg ncgcntcnnc tncatcncng cgacagctcg tacatnnngg nnccccnngn nnnantnnnn nnnnnnnccc gannaagncn anttnncnnn ntngannnnn nnnnnnnnct cnnnggnnnn nggnnttnnn ngnagnactc nccnnnnnnn nnnncnnnnn nannaannnn nnnntnnnnn ncnnnnnnnn nnnnnnnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDSSFIE NEERQALRKA VAEWASNYGS EYYLRKARAH EHTNELWSEA GKLGFLGVNL PEEYGGGGAG MYELSLVMEE MAAAGSALLL MVVSPAINGT IISKFGTEEQ KKRWLPGIAD GTLTMAFAIT EPDAGSNSHK ITTTARRDGS DWILKGQKVF ISGIDQAQAV LVVARTEEAK TGKLRPALFV VPTDAPGFSA TPIEMELISP ERQFQVFLDD VRLPSDALVG AEDAAIAQLF AGLNPERIMG AASSVGMGRF ALARAAEYVK TRQVWGTPVG AHQGLSHPLA QCHIEVELAK LMMQKAATLY DAGNDMGAAE AANMAKYAAA EAATRSVDQA VQSMGGNGLT KEYGVAAMLA SARLGRIAPV SREMVLNFVA QTSLGLPRSY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAC AGCAGCTTCA TCGAAAACGA AGAGCGTCAG GCGTTGCGCA AGGCGGTAGC CGAGTGGGCA TCCAACTACG GCAGCGAGTA CTACCTGCGG AAAGCCCGAG CCCACGAGCA CACCAACGAA TTGTGGTCCG AGGCAGGCAA ACTCGGCTTT CTCGGGGTGA ACCTGCCCGA GGAGTACGGC GGCGGGGGCG CCGGCATGTA CGAGCTGTCG CTGGTGATGG AGGAGATGGC GGCCGCGGGC AGCGCGCTGC TGTTGATGGT GGTGTCGCCG GCCATCAACG GCACCATCAT CAGCAAGTTC GGCACCGAGG AGCAGAAGAA GCGCTGGCTT CCCGGGATCG CCGACGGCAC GCTGACAATG GCGTTCGCCA TCACCGAACC CGACGCCGGG TCCAACTCGC ACAAGATCAC CACCACCGCC CGCCGCGACG GCAGCGACTG GATCCTCAAG GGCCAGAAGG TTTTCATTTC CGGCATCGAC CAGGCGCAGG CGGTGCTGGT GGTGGCCCGC ACCGAGGAGG CAAAGACCGG CAAGCTGCGG CCCGCGCTGT TCGTGGTGCC CACCGATGCG CCCGGTTTCA GCGCCACGCC GATCGAGATG GAATTGATCA GCCCGGAACG CCAGTTCCAG GTCTTCCTCG ACGACGTGCG GCTGCCGTCG GATGCGCTCG TCGGCGCCGA AGACGCCGCG ATCGCACAGC TTTTCGCCGG GCTCAACCCC GAACGCATCA TGGGCGCGGC CAGCTCCGTC GGGATGGGCC GGTTCGCGCT GGCCCGGGCC GCCGAATACG TCAAGACCCG CCAGGTGTGG GGCACCCCGG TCGGCGCGCA CCAGGGCCTG TCACATCCGT TGGCGCAGTG CCACATCGAG GTCGAACTCG CCAAGCTGAT GATGCAGAAG GCGGCCACCC TGTATGACGC CGGCAACGAC ATGGGCGCGG CCGAGGCCGC CAACATGGCC AAATATGCTG CGGCCGAGGC GGCTACCCGC TCGGTCGACC AGGCGGTGCA GTCGATGGGC GGCAACGGCC TCACCAAGGA GTACGGTGTG GCCGCCATGC TGGCCTCGGC CCGGCTGGGC CGCATCGCCC CGGTCAGCCG CGAGATGGTG CTCAACTTCG TCGCCCAGAC CTCGCTGGGC CTGCCCCGCA GCTACAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA