MythA.01263.a

Acyl-CoA dehydrogenase fadE12 (A0R3J4 homolog)

CENTER ID: MythA.01263.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01263.a.A1.GE28774 Full length( MythA.01263.a ) 1 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01263.a.A1.PW28676 Full length( MythA.01263.a ) 1 453

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3594
UniProt: G7CK72

Orthologues for Rv0972c

Species % Identity % Coverage Clones Proteins Structures
marinum 91 100 1 1 0
ulcerans 91 100 0 0 0
avium 90 100 0 1 0
paratuberculosis 90 100 1 1 0
smegmatis 82 99 1 2 0
thermoresistibile 52 98 1 1 0
thermoresistibile 37 99 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRRSPASRT DPRTAGRAGG SRCAAAAGPP GGASTGATQP ERTIESRSIR DQEGETHVSG GMHFELTEDQ ELIRASVREL AAKFDDTYWM EKDLNHEFPQ EFYDAIAGGG WLGMTIPEEY GGHGLGITEA TILAEEVARS GGGMNAASSI HLSIFGMQPV VLFGSDEMKA ATLPRIVDGS LHVCFGVTEP GAGLDTTRIT TFARREGDHY VVNGRKVWIS KAMESEKILL LARTEPLDAA ERPGTKKTDG LSLFLTDLDR DHVDIRPIKK MGRNAVGSNE LFIDDLRVPV EDRIGEEGKG FSYILHGLNP ERMLIAAEAL GIGRVALDRA VKYANERHVF DRPIGMNQGI QFPLADSLAR LDAAELVLRK ATWLYDNGKP CGREANMAKY LCADAGFAAA DRALQTHGGM GYSEEYSISR FFRESRLMKI APVSQEMILN YLGSHVLGLP RSY
NT Sequence
ATGCGAAGGC GGTCACCGGC GTCAAGGACT GACCCACGGA CGGCGGGGCG CGCCGGCGGG AGTCGGTGCG CCGCGGCGGC TGGGCCACCC GGTGGGGCCT CAACCGGTGC CACACAACCC GAGCGGACGA TCGAGAGTCG CTCCATCCGA GATCAAGAGG GAGAAACACA CGTGTCGGGC GGTATGCACT TCGAGCTGAC CGAGGATCAG GAACTCATCC GCGCCTCGGT GCGGGAGCTG GCCGCGAAGT TCGACGACAC CTATTGGATG GAGAAGGATC TCAATCACGA GTTCCCGCAG GAGTTCTACG ACGCGATCGC CGGCGGCGGA TGGCTCGGCA TGACCATCCC CGAGGAGTAC GGCGGACACG GTCTGGGCAT CACCGAGGCC ACCATCCTGG CCGAGGAGGT GGCCCGCTCG GGCGGCGGGA TGAACGCCGC GAGCTCGATC CACCTGTCCA TCTTCGGCAT GCAGCCGGTG GTGCTGTTCG GCTCCGACGA GATGAAGGCG GCCACCCTGC CCCGCATCGT CGACGGCTCG CTGCACGTCT GTTTCGGCGT CACCGAACCG GGCGCCGGCC TGGACACCAC ACGGATCACC ACCTTCGCCC GGCGCGAGGG CGACCACTAC GTCGTCAACG GACGCAAGGT GTGGATCTCC AAGGCGATGG AGTCCGAGAA GATCCTGCTG CTGGCCCGCA CCGAGCCTCT TGACGCCGCC GAAAGACCAG GCACCAAGAA GACCGACGGG TTGTCGCTGT TTCTCACCGA TCTCGACCGG GACCACGTCG ACATCCGGCC GATCAAGAAG ATGGGCCGAA ACGCCGTCGG ATCCAACGAA CTGTTCATCG ACGATCTCCG GGTTCCGGTG GAGGACCGGA TCGGTGAGGA GGGCAAGGGC TTCTCCTACA TCCTGCACGG GCTGAACCCC GAGCGGATGC TGATCGCCGC CGAGGCGCTC GGCATCGGCC GGGTCGCACT GGACCGGGCG GTCAAATACG CCAACGAACG GCATGTGTTC GACCGCCCGA TCGGGATGAA TCAGGGCATC CAGTTCCCGC TGGCCGACTC GCTGGCCCGC CTCGACGCCG CCGAACTGGT GCTGCGCAAG GCGACCTGGC TCTATGACAA CGGCAAACCC TGTGGCCGGG AAGCCAACAT GGCCAAATAC CTGTGCGCCG ACGCGGGGTT CGCCGCGGCC GACCGCGCCC TGCAGACCCA CGGCGGCATG GGGTACTCCG AGGAGTACAG CATCTCCCGT TTCTTCCGGG AATCGCGGCT GATGAAGATC GCCCCGGTGA GCCAGGAGAT GATTCTGAAC TACCTCGGGT CGCACGTCCT CGGCCTGCCC CGAAGCTAC
Details for MythA.01263.a.A1.GE28774
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 58
Validated AA Sequence
MGTLEAQTQG PGSMRRRSPA SRTDPRTAGR AGGSRCAAAA GPPGGASTGA TQPERTIESR SIRDQEGETH VSGGMHFELT EDQELIRASV RELAAKFDDT YWMEKDLNHE FPQEFYDAIA GGGWLGMTIP EEYGGHGLGI TEATILAEEV ARSGGGMNAA SSIHLSIFGM QPVVLFGSDE MKAATLPRIV DGSLHVCFGV TEPGAGLDTT RITTFARREG XHYVVNGRKV WISKAMESEK XLLLARTEPL DAAERPGTKK XDGLSLFLTD LXRXHV
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgaaggcgg tcaccggcgt caaggactga cccacggacg gcggggcgcg ccggcgggag tcggtgcgcc gcggcggctg ggccacccgg tggggcctca accggtgcca cacaacccga gcggacgatc gagagtcgct ccatccgaga tcaagaggga gaaacacacg tgtcgggcgg tatgcacttc gagctgaccg aggatcagga actcatccgc gcctcggtgc gggagctggc cgcgaagttc gacgacacct attggatgga gaaggatctc aatcacgagt tcccgcagga gttctacgac gcgatcgccg gcggcggatg gctcggcatg accatccccg aggagtacgg cggacacggt ctgggcatca ccgaggccac catcctggcc gaggaggtgg cccgctcggg cggcgggatg aacgccgcga gctcgatcca cctgtccatc ttcggcatgc agccggtggt gctgttcggc tccgacgaga tgaaggcggc caccctgccc cgcatcgtcg acggctcgct gcacgtctgt ttcggcgtca ccgaaccggg cgccggcctg gacaccacac ggatcaccac cttcgcccgg cgcgagggcg ancactacgt cgtcaacgga cgcaaggtgt ggatctccaa ggcgatggag tccgagaaga tnctgctgct ggcccgcacc gagcctcttg acgccgccga aagaccaggc accaagaaga ncgacgggtt gtcgctgttt ctcaccgatc tcgancggga ncacgtcgac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRRSPASR TDPRTAGRAG GSRCAAAAGP PGGASTGATQ PERTIESRSI RDQEGETHVS GGMHFELTED QELIRASVRE LAAKFDDTYW MEKDLNHEFP QEFYDAIAGG GWLGMTIPEE YGGHGLGITE ATILAEEVAR SGGGMNAASS IHLSIFGMQP VVLFGSDEMK AATLPRIVDG SLHVCFGVTE PGAGLDTTRI TTFARREGDH YVVNGRKVWI SKAMESEKIL LLARTEPLDA AERPGTKKTD GLSLFLTDLD RDHVDIRPIK KMGRNAVGSN ELFIDDLRVP VEDRIGEEGK GFSYILHGLN PERMLIAAEA LGIGRVALDR AVKYANERHV FDRPIGMNQG IQFPLADSLA RLDAAELVLR KATWLYDNGK PCGREANMAK YLCADAGFAA ADRALQTHGG MGYSEEYSIS RFFRESRLMK IAPVSQEMIL NYLGSHVLGL PRSY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgaaggcgg tcaccggcgt caaggactga cccacggacg gcggggcgcg ccggcgggag tcggtgcgcc gcggcggctg ggccacccgg tggggcctca accggtgcca cacaacccga gcggacgatc gagagtcgct ccatccgaga tcaagaggga gaaacacacg tgtcgggcgg tatgcacttc gagctgaccg aggatcagga actcatccgc gcctcggtgc gggagctggc cgcgaagttc gacgacacct attggatgga gaaggatctc aatcacgagt tcccgcagga gttctacgac gcgatcgccg gcggcggatg gctcggcatg accatccccg aggagtacgg cggacacggt ctgggcatca ccgaggccac catcctggcc gaggaggtgg cccgctcggg cggcgggatg aacgccgcga gctcgatcca cctgtccatc ttcggcatgc agccggtggt gctgttcggc tccgacgaga tgaaggcggc caccctgccc cgcatcgtcg acggctcgct gcacgtctgt ttcggcgtca ccgaaccggg cgccggcctg gacaccacac ggatcaccac cttcgcccgg cgcgagggcg accactacgt cgtcaacgga cgcaaggtgt ggatctccaa ggcgatggag tccgagaaga tcctgctgct ggcccgcacc gagcctcttg acgccgccga aagaccaggc accaagaaga ccgacgggtt gtcgctgttt ctcaccgatc tcgaccggga ccacgtcgac atccggccga tcaagaagat gggccgaaac gccgtcggat ccaacgaact gttcatcgac gatctccggg ttccggtgga ggaccggatc ggtgaggagg gcaagggctt ctcctacatc ctgcacgggc tgaaccccga gcggatgctg atcgccgccg aggcgctcgg catcggccgg gtcgcactgg accgggcggt caaatacgcc aacgaacggc atgtgttcga ccgcccgatc gggatgaatc agggcatcca gttcccgctg gccgactcgc tggcccgcct cgacgccgcc gaactggtgc tgcgcaaggc gacctggctc tatgacaacg gcaaaccctg tggccgggaa gccaacatgg ccaaatacct gtgcgccgac gcggggttcg ccgcggccga ccgcgccctg cagacccacg gcggcatggg gtactccgag gagtacagca tctcccgttt cttccgggaa tcgcggctga tgaagatcgc cccggtgagc caggagatga ttctgaacta cctcgggtcg cacgtcctcg gcctgccccg aagctacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MythA.01263.a.A1.PW28676
PURIFICATION DATe: 6/21/2010
CONCENTRATION: 25.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMRRRSPA SRTDPRTAGR AGGSRCAAAA GPPGGASTGA TQPERTIESR SIRDQEGETH VSGGMHFELT EDQELIRASV RELAAKFDDT YWMEKDLNHE FPQEFYDAIA GGGWLGMTIP EEYGGHGLGI TEATILAEEV ARSGGGMNAA SSIHLSIFGM QPVVLFGSDE MKAATLPRIV DGSLHVCFGV TEPGAGLDTT RITTFARREG XHYVVNGRKV WISKAMESEK XLLLARTEPL DAAERPGTKK XDGLSLFLTD LXRXHV
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgaaggcgg tcaccggcgt caaggactga cccacggacg gcggggcgcg ccggcgggag tcggtgcgcc gcggcggctg ggccacccgg tggggcctca accggtgcca cacaacccga gcggacgatc gagagtcgct ccatccgaga tcaagaggga gaaacacacg tgtcgggcgg tatgcacttc gagctgaccg aggatcagga actcatccgc gcctcggtgc gggagctggc cgcgaagttc gacgacacct attggatgga gaaggatctc aatcacgagt tcccgcagga gttctacgac gcgatcgccg gcggcggatg gctcggcatg accatccccg aggagtacgg cggacacggt ctgggcatca ccgaggccac catcctggcc gaggaggtgg cccgctcggg cggcgggatg aacgccgcga gctcgatcca cctgtccatc ttcggcatgc agccggtggt gctgttcggc tccgacgaga tgaaggcggc caccctgccc cgcatcgtcg acggctcgct gcacgtctgt ttcggcgtca ccgaaccggg cgccggcctg gacaccacac ggatcaccac cttcgcccgg cgcgagggcg ancactacgt cgtcaacgga cgcaaggtgt ggatctccaa ggcgatggag tccgagaaga tnctgctgct ggcccgcacc gagcctcttg acgccgccga aagaccaggc accaagaaga ncgacgggtt gtcgctgttt ctcaccgatc tcgancggga ncacgtcgac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRRSPASR TDPRTAGRAG GSRCAAAAGP PGGASTGATQ PERTIESRSI RDQEGETHVS GGMHFELTED QELIRASVRE LAAKFDDTYW MEKDLNHEFP QEFYDAIAGG GWLGMTIPEE YGGHGLGITE ATILAEEVAR SGGGMNAASS IHLSIFGMQP VVLFGSDEMK AATLPRIVDG SLHVCFGVTE PGAGLDTTRI TTFARREGDH YVVNGRKVWI SKAMESEKIL LLARTEPLDA AERPGTKKTD GLSLFLTDLD RDHVDIRPIK KMGRNAVGSN ELFIDDLRVP VEDRIGEEGK GFSYILHGLN PERMLIAAEA LGIGRVALDR AVKYANERHV FDRPIGMNQG IQFPLADSLA RLDAAELVLR KATWLYDNGK PCGREANMAK YLCADAGFAA ADRALQTHGG MGYSEEYSIS RFFRESRLMK IAPVSQEMIL NYLGSHVLGL PRSY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGAAGGCGG TCACCGGCGT CAAGGACTGA CCCACGGACG GCGGGGCGCG CCGGCGGGAG TCGGTGCGCC GCGGCGGCTG GGCCACCCGG TGGGGCCTCA ACCGGTGCCA CACAACCCGA GCGGACGATC GAGAGTCGCT CCATCCGAGA TCAAGAGGGA GAAACACACG TGTCGGGCGG TATGCACTTC GAGCTGACCG AGGATCAGGA ACTCATCCGC GCCTCGGTGC GGGAGCTGGC CGCGAAGTTC GACGACACCT ATTGGATGGA GAAGGATCTC AATCACGAGT TCCCGCAGGA GTTCTACGAC GCGATCGCCG GCGGCGGATG GCTCGGCATG ACCATCCCCG AGGAGTACGG CGGACACGGT CTGGGCATCA CCGAGGCCAC CATCCTGGCC GAGGAGGTGG CCCGCTCGGG CGGCGGGATG AACGCCGCGA GCTCGATCCA CCTGTCCATC TTCGGCATGC AGCCGGTGGT GCTGTTCGGC TCCGACGAGA TGAAGGCGGC CACCCTGCCC CGCATCGTCG ACGGCTCGCT GCACGTCTGT TTCGGCGTCA CCGAACCGGG CGCCGGCCTG GACACCACAC GGATCACCAC CTTCGCCCGG CGCGAGGGCG ACCACTACGT CGTCAACGGA CGCAAGGTGT GGATCTCCAA GGCGATGGAG TCCGAGAAGA TCCTGCTGCT GGCCCGCACC GAGCCTCTTG ACGCCGCCGA AAGACCAGGC ACCAAGAAGA CCGACGGGTT GTCGCTGTTT CTCACCGATC TCGACCGGGA CCACGTCGAC ATCCGGCCGA TCAAGAAGAT GGGCCGAAAC GCCGTCGGAT CCAACGAACT GTTCATCGAC GATCTCCGGG TTCCGGTGGA GGACCGGATC GGTGAGGAGG GCAAGGGCTT CTCCTACATC CTGCACGGGC TGAACCCCGA GCGGATGCTG ATCGCCGCCG AGGCGCTCGG CATCGGCCGG GTCGCACTGG ACCGGGCGGT CAAATACGCC AACGAACGGC ATGTGTTCGA CCGCCCGATC GGGATGAATC AGGGCATCCA GTTCCCGCTG GCCGACTCGC TGGCCCGCCT CGACGCCGCC GAACTGGTGC TGCGCAAGGC GACCTGGCTC TATGACAACG GCAAACCCTG TGGCCGGGAA GCCAACATGG CCAAATACCT GTGCGCCGAC GCGGGGTTCG CCGCGGCCGA CCGCGCCCTG CAGACCCACG GCGGCATGGG GTACTCCGAG GAGTACAGCA TCTCCCGTTT CTTCCGGGAA TCGCGGCTGA TGAAGATCGC CCCGGTGAGC CAGGAGATGA TTCTGAACTA CCTCGGGTCG CACGTCCTCG GCCTGCCCCG AAGCTACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA