SSGCID: Target Page

MymaA.01263.a

Acyl-CoA dehydrogenase FadE12

CENTER ID:	MymaA.01263.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01263.a.A1.GE30162	Full length( MymaA.01263.a )		1	388

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01263.a.A1.PW30035	Full length( MymaA.01263.a )		1	388

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.4872
RefSeq:	YP_001852796.1
UniProt:	B2HEB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDTSFIENE DRRALRKAVS EWASNYGHEY YLKKARAHEH TTELWSEAGK LGFIGVNLPE EYGGGGAGMY ELSMVMEEMA AAGSALLLMV VSPAINGTII SKFGTDEQKQ RWLPGIADGT LTMAFAITEP DAGSNSHKIT TTARRDGSDW IIKGQKVYIS GIDQAQAVLV VGRTEEAKTG KLRPALFVVP TDTPGFTYTA IDMELVSPER QFQVFLDDVR LPADALVGAQ DAAIAQLFAG LNPERIMGAA SAVGMGRFAL NRAVDYVKTR QVWSTPIGAH QGLAHPLAQC HIEVELAKLM MQKAATLYDH GDDGGAAEAA NMAKYAAAEA SARSVDQAVQ SMGGNGLTQE YGVAAMLTSS RLARIAPISR EMVLNFVAQT SLGLPRSY

NT Sequence

ATGACCGACA CCAGCTTCAT CGAGAATGAG GACCGCCGGG CGCTACGCAA GGCAGTATCG GAATGGGCGT CCAACTACGG CCACGAGTAC TATCTGAAGA AGGCGCGTGC TCATGAGCAC ACCACCGAGC TATGGTCGGA GGCAGGCAAA CTCGGCTTCA TCGGGGTGAA CCTGCCCGAG GAGTACGGCG GCGGCGGCGC CGGCATGTAC GAGCTGTCGA TGGTGATGGA GGAAATGGCC GCGGCCGGCT CCGCGCTGTT GCTGATGGTG GTCTCGCCGG CCATCAACGG CACCATCATC AGCAAGTTCG GCACCGACGA GCAGAAGCAG CGCTGGCTTC CCGGCATCGC GGACGGGACC CTGACGATGG CCTTCGCCAT CACCGAGCCC GACGCCGGAT CCAACTCGCA CAAGATCACC ACGACCGCCC GCCGTGACGG CAGCGACTGG ATCATCAAGG GGCAGAAGGT CTATATCTCC GGTATTGACC AAGCCCAAGC GGTGCTGGTG GTGGGACGCA CCGAGGAAGC CAAGACCGGG AAGCTGCGTC CGGCGTTGTT CGTGGTACCC ACCGACACCC CCGGCTTCAC CTACACGGCG ATCGACATGG AACTGGTCAG CCCCGAACGC CAGTTCCAGG TTTTCCTCGA TGATGTGCGG CTGCCCGCAG ACGCGCTGGT CGGAGCCCAG GACGCGGCCA TCGCCCAGCT TTTCGCCGGG CTGAATCCCG AGCGCATCAT GGGTGCGGCC AGCGCGGTCG GCATGGGGCG GTTCGCACTG AACCGGGCCG TCGACTACGT CAAGACGCGC CAGGTGTGGT CCACCCCGAT CGGCGCCCAC CAGGGGTTGG CGCATCCCTT GGCGCAATGC CACATCGAAG TCGAGCTCGC CAAGCTGATG ATGCAGAAGG CGGCGACGCT GTATGACCAT GGCGATGACG GCGGAGCGGC CGAGGCCGCC AACATGGCCA AGTACGCGGC GGCGGAGGCG TCGGCCCGCT CGGTCGATCA GGCCGTGCAG TCGATGGGCG GCAACGGGCT GACCCAGGAG TACGGGGTCG CGGCCATGCT GACCTCGTCG CGCTTGGCCC GGATTGCCCC GATCAGCCGT GAGATGGTGC TCAACTTCGT CGCGCAGACG TCGCTGGGCC TGCCCCGAAG CTAC

Details for MymaA.01263.a.A1.GE30162

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	43kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

agtagcttcg gggcaggccc agcgacgtct gcgcgacgaa gttgagcacc atctcacggc tgatcggggc aatccgggcc aagcgcgacg aggtcagcat ggccgcgacc ccgtactcct gggtcagccc gttgccgccc atcgactgca cggcctgatc gaccgagcgg gccgacgcct ccgccgccgc gtacttggcc atgttggcgg cctcggccgc tccgccgtca tcgccatggt catacagcgt cgccgccttc tgcatcatca gcttggcgag ctcgacttcg atgtggcatt gcgccaaggg atgcgccaac ccctggtggg cgccgatcgg ggtggaccac acctggcgcg tcttgacgta gtcgacggcc cggttcagtg cgaaccgccc catgccgacc gcgctggccg cacccatgat gcgctcggga ttcagcccgg cgaaaagctg ggcgatggcc gcgtcctggg ctccgaccag cgcgtctgcg ggcagccgca catcatcgag gaaaacctgg aactggcgtt cggggctgac cagttccatg tcgatcgccg tgtaggtgaa gccgggggtg tcggtgggta ccacgaacaa cgccggacgc agcttcccgg tcttggcttc ctcggtgcgt cccaccacca gcaccgcttg ggcttggtca ataccggaga tatagacctt ctgccccttg atgatccagt cgctgccgtc acggcgggcg gtcgtggtga tcttgtgcga gttggatccg gcgtcgggct cggtgatggc gaaggccatc gtcagggtcc cgtccgcgat gccgggaagc cagcgctgct tctgctcgtc ggtgccgaac ttgctgatga tggtgccgtt gatggccggc gagaccacca tcagcaacag cgcggagccg gccgcggcca tttcctccat caccatcgac agctcgtaca tgccggcgcc gccgccgccg tactcctcgg gcaggttcac cccgatgaag ccgagtttgc ctgcctccga ccatagctcg gtggtgtgct catgagcacg cgccttcttc agatagtact cgtggccgta gttggacgcc cattccgata ctgccttgcg tagcgcccgg cggtcctcat tctcgatgaa gctggtgtcg gtcatcgaac caggaccctg ggtctgagct tccagggtac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTDTSFIEN EDRRALRKAV SEWASNYGHE YYLKKARAHE HTTELWSEAG KLGFIGVNLP EEYGGGGAGM YELSMVMEEM AAAGSALLLM VVSPAINGTI ISKFGTDEQK QRWLPGIADG TLTMAFAITE PDAGSNSHKI TTTARRDGSD WIIKGQKVYI SGIDQAQAVL VVGRTEEAKT GKLRPALFVV PTDTPGFTYT AIDMELVSPE RQFQVFLDDV RLPADALVGA QDAAIAQLFA GLNPERIMGA ASAVGMGRFA LNRAVDYVKT RQVWSTPIGA HQGLAHPLAQ CHIEVELAKL MMQKAATLYD HGDDGGAAEA ANMAKYAAAE ASARSVDQAV QSMGGNGLTQ EYGVAAMLTS SRLARIAPIS REMVLNFVAQ TSLGLPRSY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgacacc agcttcatcg agaatgagga ccgccgggcg ctacgcaagg cagtatcgga atgggcgtcc aactacggcc acgagtacta tctgaagaag gcgcgtgctc atgagcacac caccgagcta tggtcggagg caggcaaact cggcttcatc ggggtgaacc tgcccgagga gtacggcggc ggcggcgccg gcatgtacga gctgtcgatg gtgatggagg aaatggccgc ggccggctcc gcgctgttgc tgatggtggt ctcgccggcc atcaacggca ccatcatcag caagttcggc accgacgagc agaagcagcg ctggcttccc ggcatcgcgg acgggaccct gacgatggcc ttcgccatca ccgagcccga cgccggatcc aactcgcaca agatcaccac gaccgcccgc cgtgacggca gcgactggat catcaagggg cagaaggtct atatctccgg tattgaccaa gcccaagcgg tgctggtggt gggacgcacc gaggaagcca agaccgggaa gctgcgtccg gcgttgttcg tggtacccac cgacaccccc ggcttcacct acacggcgat cgacatggaa ctggtcagcc ccgaacgcca gttccaggtt ttcctcgatg atgtgcggct gcccgcagac gcgctggtcg gagcccagga cgcggccatc gcccagcttt tcgccgggct gaatcccgag cgcatcatgg gtgcggccag cgcggtcggc atggggcggt tcgcactgaa ccgggccgtc gactacgtca agacgcgcca ggtgtggtcc accccgatcg gcgcccacca ggggttggcg catcccttgg cgcaatgcca catcgaagtc gagctcgcca agctgatgat gcagaaggcg gcgacgctgt atgaccatgg cgatgacggc ggagcggccg aggccgccaa catggccaag tacgcggcgg cggaggcgtc ggcccgctcg gtcgatcagg ccgtgcagtc gatgggcggc aacgggctga cccaggagta cggggtcgcg gccatgctga cctcgtcgcg cttggcccgg attgccccga tcagccgtga gatggtgctc aacttcgtcg cgcagacgtc gctgggcctg ccccgaagct acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MymaA.01263.a.A1.PW30035

PURIFICATION DATe:	11/15/2010
CONCENTRATION:	27.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGACACC AGCTTCATCG AGAATGAGGA CCGCCGGGCG CTACGCAAGG CAGTATCGGA ATGGGCGTCC AACTACGGCC ACGAGTACTA TCTGAAGAAG GCGCGTGCTC ATGAGCACAC CACCGAGCTA TGGTCGGAGG CAGGCAAACT CGGCTTCATC GGGGTGAACC TGCCCGAGGA GTACGGCGGC GGCGGCGCCG GCATGTACGA GCTGTCGATG GTGATGGAGG AAATGGCCGC GGCCGGCTCC GCGCTGTTGC TGATGGTGGT CTCGCCGGCC ATCAACGGCA CCATCATCAG CAAGTTCGGC ACCGACGAGC AGAAGCAGCG CTGGCTTCCC GGCATCGCGG ACGGGACCCT GACGATGGCC TTCGCCATCA CCGAGCCCGA CGCCGGATCC AACTCGCACA AGATCACCAC GACCGCCCGC CGTGACGGCA GCGACTGGAT CATCAAGGGG CAGAAGGTCT ATATCTCCGG TATTGACCAA GCCCAAGCGG TGCTGGTGGT GGGACGCACC GAGGAAGCCA AGACCGGGAA GCTGCGTCCG GCGTTGTTCG TGGTACCCAC CGACACCCCC GGCTTCACCT ACACGGCGAT CGACATGGAA CTGGTCAGCC CCGAACGCCA GTTCCAGGTT TTCCTCGATG ATGTGCGGCT GCCCGCAGAC GCGCTGGTCG GAGCCCAGGA CGCGGCCATC GCCCAGCTTT TCGCCGGGCT GAATCCCGAG CGCATCATGG GTGCGGCCAG CGCGGTCGGC ATGGGGCGGT TCGCACTGAA CCGGGCCGTC GACTACGTCA AGACGCGCCA GGTGTGGTCC ACCCCGATCG GCGCCCACCA GGGGTTGGCG CATCCCTTGG CGCAATGCCA CATCGAAGTC GAGCTCGCCA AGCTGATGAT GCAGAAGGCG GCGACGCTGT ATGACCATGG CGATGACGGC GGAGCGGCCG AGGCCGCCAA CATGGCCAAG TACGCGGCGG CGGAGGCGTC GGCCCGCTCG GTCGATCAGG CCGTGCAGTC GATGGGCGGC AACGGGCTGA CCCAGGAGTA CGGGGTCGCG GCCATGCTGA CCTCGTCGCG CTTGGCCCGG ATTGCCCCGA TCAGCCGTGA GATGGTGCTC AACTTCGTCG CGCAGACGTC GCTGGGCCTG CCCCGAAGCT ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA