MysmA.01263.b

Acyl-CoA dehydrogenase fadE12

CENTER ID: MysmA.01263.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01263.b.A1.GE28898 Full length( MysmA.01263.b ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01263.b.A1.PS00702 Full length( MysmA.01263.b ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5354
RefSeq: YP_889732.1
UniProt: A0R3J4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGFVETEEQ QALRRAVAAM AANYGQDYYL EKARAGQHTT ELWNEAGKLG FIGVNLPEEY GGGGAGMYEL SLVMEEMAAT GCALLMMVVS PAINGTIISK FGTEEQKRRW LPGIADGSIT MAFAITEPDA GSNSHRITTT ARRDGSDWIL KGQKVFISGI DQAQAVLVVG RTEDHKTGNL KPALFVIPTD TPGLHWTKIE MELISPESQF QVFLDDVRVP ADALVGSEDA ALAQLFAGLN PERIMGAASA VGMGRFAIHK AVDYVKTRQV WKVPIGSHQG LSHPLAQNHI EIELAKLMMQ KAAALYDAGD DLGAAEAANM AKYAAGEASV RAVDQAVQSL GGNGLTKEYG IAAAVTASKL ARIAPVSREM ILNFVSQTSL GLPRSY
NT Sequence
ATGAGCGGTT TCGTCGAGAC CGAGGAACAG CAGGCGCTGC GCCGCGCTGT CGCGGCCATG GCGGCCAACT ACGGGCAGGA CTATTACCTG GAGAAGGCCC GCGCCGGCCA ACACACCACC GAATTGTGGA ACGAGGCGGG CAAACTCGGC TTCATCGGGG TGAACCTGCC CGAGGAGTAC GGCGGCGGCG GGGCAGGCAT GTACGAACTC AGCCTGGTCA TGGAAGAGAT GGCCGCCACC GGGTGCGCCC TGCTGATGAT GGTGGTCTCC CCCGCGATCA ACGGCACCAT CATCTCCAAG TTCGGCACCG AGGAGCAGAA GAGGCGCTGG CTTCCCGGCA TCGCCGACGG GTCGATCACC ATGGCATTCG CCATCACCGA ACCCGACGCC GGATCCAACA GCCACCGCAT CACCACCACG GCCCGCCGCG ACGGTAGCGA CTGGATCCTC AAGGGTCAGA AGGTGTTCAT CTCCGGCATC GACCAGGCGC AGGCCGTGCT GGTGGTGGGC CGCACGGAAG ATCACAAGAC CGGCAATCTG AAGCCCGCAC TGTTCGTGAT CCCCACCGAC ACCCCGGGAC TGCACTGGAC GAAGATCGAG ATGGAGTTGA TCAGCCCGGA GAGCCAGTTC CAGGTGTTCC TCGACGATGT GCGGGTACCC GCCGATGCGC TGGTGGGTTC CGAGGACGCC GCGCTCGCGC AACTGTTCGC CGGCCTCAAT CCCGAGCGCA TCATGGGTGC GGCCAGTGCG GTGGGCATGG GCCGGTTCGC GATCCACAAG GCCGTCGATT ACGTCAAGAC CCGCCAGGTC TGGAAGGTGC CGATCGGCTC GCACCAGGGT CTGTCACATC CGCTGGCGCA GAACCACATC GAGATCGAAC TCGCCAAGCT GATGATGCAG AAGGCCGCGG CACTGTACGA CGCGGGTGAC GATCTCGGGG CCGCCGAGGC CGCCAACATG GCCAAGTACG CCGCAGGCGA GGCCTCGGTC CGTGCGGTCG ACCAGGCCGT GCAGTCCCTG GGCGGCAACG GTTTGACCAA GGAGTACGGC ATCGCCGCGG CGGTCACGGC ATCGAAGTTG GCGCGCATCG CACCGGTCAG TCGCGAGATG ATCCTCAACT TCGTCTCGCA GACCTCACTC GGACTCCCGC GCTCATAC
Details for MysmA.01263.b.A1.GE28898
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFVETEE QQALRRAVAA MAANYGQDYY LEKARAGQHT TELWNEAGKL GFIGVNLPEE YGGGGAGMYE LSLVMEEMAA TGCALLMMVV SPAINGTIIS KFGTEEQKRR WLPGIADGSI TMAFAITEPD AGSNSHRITT TARRDGSDWI LKGQKVFISG IDQAQAVLVV GRTEDHKTGN LKPALFVIPT DTPGLHWTKI EMELISPESQ FQVFLDDVRV PADALVGSED AALAQLFAGL NPERIMGAAS AVGMGRFAIH KAVDYVKTRQ VWKVPIGSHQ GLSHPLAQNH IEIELAKLMM QKAAALYDAG DDLGAAEAAN MAKYAAGEAS VRAVDQAVQS LGGNGLTKEY GIAAAVTASK LARIAPVSRE MIXNFVSQTS LGL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg gtttcgtcga gaccgaggaa cagcaggcgc tgcgccgcgc tgtcgcggcc atggcggcca actacgggca ggactattac ctggagaagg cccgcgccgg ccaacacacc accgaattgt ggaacgaggc gggcaaactc ggcttcatcg gggtgaacct gcccgaggag tacggcggcg gcggggcagg catgtacgaa ctcagcctgg tcatggaaga gatggccgcc accgggtgcg ccctgctgat gatggtggtc tcccccgcga tcaacggcac catcatctcc aagttcggca ccgaggagca gaagaggcgc tggcttcccg gcatcgccga cgggtcgatc accatggcat tcgccatcac cgaacccgac gccggatcca acagccaccg catcaccacc acggcccgcc gcgacggtag cgactggatc ctcaagggtc agaaggtgtt catctccggc atcgaccagg cgcaggccgt gctggtggtg ggccgcacgg aagatcacaa gaccggcaat ctgaagcccg cactgttcgt gatccccacc gacaccccgg gactgcactg gacgaagatc gagatggagt tgatcagccc ggagagccag ttccaggtgt tcctcgacga tgtgcgggta cccgccgatg cgctggtggg ttccgaggac gccgcgctcg cgcaactgtt cgccggcctc aatcccgagc gcatcatggg tgcggccagt gcggtgggca tgggccggtt cgcgatccac aaggccgtcg attacgtcaa gacccgccag gtctggaagg tgccgatcgg ctcgcaccag ggtctgtcac atccgctggc gcagaaccac atcgagatcg aactcgccaa gctgatgatg cagaaggccg cggcactgta cgacgcgggt gacgatctcg gggccgccga ggccgccaac atggccaagt acgccgcagg cgaggcctcg gtccgtgcgg tcgaccaggc cgtgcagtcc ctgggcggca acggtttgac caaggagtac ggcatcgccg cggcggtcac ggcatcgaag ttggcgcgca tcgcaccggt cagtcgcgag atgatcntca acttcgtctc gcagacctca ctcggactcc cg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFVETEE QQALRRAVAA MAANYGQDYY LEKARAGQHT TELWNEAGKL GFIGVNLPEE YGGGGAGMYE LSLVMEEMAA TGCALLMMVV SPAINGTIIS KFGTEEQKRR WLPGIADGSI TMAFAITEPD AGSNSHRITT TARRDGSDWI LKGQKVFISG IDQAQAVLVV GRTEDHKTGN LKPALFVIPT DTPGLHWTKI EMELISPESQ FQVFLDDVRV PADALVGSED AALAQLFAGL NPERIMGAAS AVGMGRFAIH KAVDYVKTRQ VWKVPIGSHQ GLSHPLAQNH IEIELAKLMM QKAAALYDAG DDLGAAEAAN MAKYAAGEAS VRAVDQAVQS LGGNGLTKEY GIAAAVTASK LARIAPVSRE MILNFVSQTS LGLPRSY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcggtttc gtcgagaccg aggaacagca ggcgctgcgc cgcgctgtcg cggccatggc ggccaactac gggcaggact attacctgga gaaggcccgc gccggccaac acaccaccga attgtggaac gaggcgggca aactcggctt catcggggtg aacctgcccg aggagtacgg cggcggcggg gcaggcatgt acgaactcag cctggtcatg gaagagatgg ccgccaccgg gtgcgccctg ctgatgatgg tggtctcccc cgcgatcaac ggcaccatca tctccaagtt cggcaccgag gagcagaaga ggcgctggct tcccggcatc gccgacgggt cgatcaccat ggcattcgcc atcaccgaac ccgacgccgg atccaacagc caccgcatca ccaccacggc ccgccgcgac ggtagcgact ggatcctcaa gggtcagaag gtgttcatct ccggcatcga ccaggcgcag gccgtgctgg tggtgggccg cacggaagat cacaagaccg gcaatctgaa gcccgcactg ttcgtgatcc ccaccgacac cccgggactg cactggacga agatcgagat ggagttgatc agcccggaga gccagttcca ggtgttcctc gacgatgtgc gggtacccgc cgatgcgctg gtgggttccg aggacgccgc gctcgcgcaa ctgttcgccg gcctcaatcc cgagcgcatc atgggtgcgg ccagtgcggt gggcatgggc cggttcgcga tccacaaggc cgtcgattac gtcaagaccc gccaggtctg gaaggtgccg atcggctcgc accagggtct gtcacatccg ctggcgcaga accacatcga gatcgaactc gccaagctga tgatgcagaa ggccgcggca ctgtacgacg cgggtgacga tctcggggcc gccgaggccg ccaacatggc caagtacgcc gcaggcgagg cctcggtccg tgcggtcgac caggccgtgc agtccctggg cggcaacggt ttgaccaagg agtacggcat cgccgcggcg gtcacggcat cgaagttggc gcgcatcgca ccggtcagtc gcgagatgat cctcaacttc gtctcgcaga cctcactcgg actcccgcgc tcatacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MysmA.01263.b.A1.PS00702
PURIFICATION DATe: 7/28/2010
CONCENTRATION: 50.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFVETEE QQALRRAVAA MAANYGQDYY LEKARAGQHT TELWNEAGKL GFIGVNLPEE YGGGGAGMYE LSLVMEEMAA TGCALLMMVV SPAINGTIIS KFGTEEQKRR WLPGIADGSI TMAFAITEPD AGSNSHRITT TARRDGSDWI LKGQKVFISG IDQAQAVLVV GRTEDHKTGN LKPALFVIPT DTPGLHWTKI EMELISPESQ FQVFLDDVRV PADALVGSED AALAQLFAGL NPERIMGAAS AVGMGRFAIH KAVDYVKTRQ VWKVPIGSHQ GLSHPLAQNH IEIELAKLMM QKAAALYDAG DDLGAAEAAN MAKYAAGEAS VRAVDQAVQS LGGNGLTKEY GIAAAVTASK LARIAPVSRE MIXNFVSQTS LGL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg gtttcgtcga gaccgaggaa cagcaggcgc tgcgccgcgc tgtcgcggcc atggcggcca actacgggca ggactattac ctggagaagg cccgcgccgg ccaacacacc accgaattgt ggaacgaggc gggcaaactc ggcttcatcg gggtgaacct gcccgaggag tacggcggcg gcggggcagg catgtacgaa ctcagcctgg tcatggaaga gatggccgcc accgggtgcg ccctgctgat gatggtggtc tcccccgcga tcaacggcac catcatctcc aagttcggca ccgaggagca gaagaggcgc tggcttcccg gcatcgccga cgggtcgatc accatggcat tcgccatcac cgaacccgac gccggatcca acagccaccg catcaccacc acggcccgcc gcgacggtag cgactggatc ctcaagggtc agaaggtgtt catctccggc atcgaccagg cgcaggccgt gctggtggtg ggccgcacgg aagatcacaa gaccggcaat ctgaagcccg cactgttcgt gatccccacc gacaccccgg gactgcactg gacgaagatc gagatggagt tgatcagccc ggagagccag ttccaggtgt tcctcgacga tgtgcgggta cccgccgatg cgctggtggg ttccgaggac gccgcgctcg cgcaactgtt cgccggcctc aatcccgagc gcatcatggg tgcggccagt gcggtgggca tgggccggtt cgcgatccac aaggccgtcg attacgtcaa gacccgccag gtctggaagg tgccgatcgg ctcgcaccag ggtctgtcac atccgctggc gcagaaccac atcgagatcg aactcgccaa gctgatgatg cagaaggccg cggcactgta cgacgcgggt gacgatctcg gggccgccga ggccgccaac atggccaagt acgccgcagg cgaggcctcg gtccgtgcgg tcgaccaggc cgtgcagtcc ctgggcggca acggtttgac caaggagtac ggcatcgccg cggcggtcac ggcatcgaag ttggcgcgca tcgcaccggt cagtcgcgag atgatcntca acttcgtctc gcagacctca ctcggactcc cg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFVETEE QQALRRAVAA MAANYGQDYY LEKARAGQHT TELWNEAGKL GFIGVNLPEE YGGGGAGMYE LSLVMEEMAA TGCALLMMVV SPAINGTIIS KFGTEEQKRR WLPGIADGSI TMAFAITEPD AGSNSHRITT TARRDGSDWI LKGQKVFISG IDQAQAVLVV GRTEDHKTGN LKPALFVIPT DTPGLHWTKI EMELISPESQ FQVFLDDVRV PADALVGSED AALAQLFAGL NPERIMGAAS AVGMGRFAIH KAVDYVKTRQ VWKVPIGSHQ GLSHPLAQNH IEIELAKLMM QKAAALYDAG DDLGAAEAAN MAKYAAGEAS VRAVDQAVQS LGGNGLTKEY GIAAAVTASK LARIAPVSRE MILNFVSQTS LGLPRSY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGTTTC GTCGAGACCG AGGAACAGCA GGCGCTGCGC CGCGCTGTCG CGGCCATGGC GGCCAACTAC GGGCAGGACT ATTACCTGGA GAAGGCCCGC GCCGGCCAAC ACACCACCGA ATTGTGGAAC GAGGCGGGCA AACTCGGCTT CATCGGGGTG AACCTGCCCG AGGAGTACGG CGGCGGCGGG GCAGGCATGT ACGAACTCAG CCTGGTCATG GAAGAGATGG CCGCCACCGG GTGCGCCCTG CTGATGATGG TGGTCTCCCC CGCGATCAAC GGCACCATCA TCTCCAAGTT CGGCACCGAG GAGCAGAAGA GGCGCTGGCT TCCCGGCATC GCCGACGGGT CGATCACCAT GGCATTCGCC ATCACCGAAC CCGACGCCGG ATCCAACAGC CACCGCATCA CCACCACGGC CCGCCGCGAC GGTAGCGACT GGATCCTCAA GGGTCAGAAG GTGTTCATCT CCGGCATCGA CCAGGCGCAG GCCGTGCTGG TGGTGGGCCG CACGGAAGAT CACAAGACCG GCAATCTGAA GCCCGCACTG TTCGTGATCC CCACCGACAC CCCGGGACTG CACTGGACGA AGATCGAGAT GGAGTTGATC AGCCCGGAGA GCCAGTTCCA GGTGTTCCTC GACGATGTGC GGGTACCCGC CGATGCGCTG GTGGGTTCCG AGGACGCCGC GCTCGCGCAA CTGTTCGCCG GCCTCAATCC CGAGCGCATC ATGGGTGCGG CCAGTGCGGT GGGCATGGGC CGGTTCGCGA TCCACAAGGC CGTCGATTAC GTCAAGACCC GCCAGGTCTG GAAGGTGCCG ATCGGCTCGC ACCAGGGTCT GTCACATCCG CTGGCGCAGA ACCACATCGA GATCGAACTC GCCAAGCTGA TGATGCAGAA GGCCGCGGCA CTGTACGACG CGGGTGACGA TCTCGGGGCC GCCGAGGCCG CCAACATGGC CAAGTACGCC GCAGGCGAGG CCTCGGTCCG TGCGGTCGAC CAGGCCGTGC AGTCCCTGGG CGGCAACGGT TTGACCAAGG AGTACGGCAT CGCCGCGGCG GTCACGGCAT CGAAGTTGGC GCGCATCGCA CCGGTCAGTC GCGAGATGAT CCTCAACTTC GTCTCGCAGA CCTCACTCGG ACTCCCGCGC TCATACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA