SSGCID: Target Page

MythA.00318.a

TatD family hydrolase (Rv1008 ortholog)

CENTER ID:	MythA.00318.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00318.a.B1.GE37638	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.648
RefSeq:	WP_003924100.1
UniProt:	G7CCF9

Orthologues for Rv1008

Species	% Identity	% Coverage	Clones
avium	83	98	1
leprae	83	98	1
marinum	83	98	1
ulcerans	83	98	0
thermoresistibile	79	98	1
fortuitum	76	98	1
abscessus	70	98	1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSSSTRPTE RPKPPAPEPL TPLVDAHTHL DACGARDADD VRRIMNRAGA AGVRAAVTIA DDLASARWVT RAAEWDSRVY AAVALHPTRA DALTDQARAE LEALAAHPRV VAIGETGMDL YWPGRLDGCA DPAAQREAFA WHIDLAKRTG KPLMIHNREA DAEVLDVLRA EGAPETVIMH CFSSDAVMAR TCVEAGWVLS LSGTVSFRNA RALHEAAPLI PPGQMLVETD APYLTPHPYR GAPNEPYCLP YTVRALADLL GRPAEDVAWE TADTAVSVYG LDESCRI

NT Sequence

gtgaccagct cgtccacccg cccgaccgaa cggcccaagc cgccggctcc cgagccgctg accccgctgg tcgacgcgca cacccacctc gacgcctgcg gcgcccggga cgccgacgac gtgcggcgga tcatgaaccg cgccggtgcg gccggggtgc gggccgcggt caccatcgcc gatgatctgg cgtcggcgcg ctgggtgacc agggccgcgg agtgggattc gcgggtgtat gcggcggtgg cgctgcaccc cacccgcgcc gacgccctga ccgaccaggc ccgcgccgag ctcgaggcgc tggccgcgca cccccgggtg gtggcgatcg gggagaccgg tatggacctg tactggcccg gccgcctcga cgggtgcgcc gatccggccg cccagcgcga ggccttcgcc tggcacatcg acctggccaa acgcaccggg aaaccactga tgatccacaa ccgcgaggcc gatgccgagg tgctcgacgt gctgcgcgcg gagggggcgc cggagacggt gatcatgcac tgcttctcgt ccgacgcggt gatggcacgg acctgtgtgg aggccgggtg ggtgctcagc ctgtccggca ccgtctcgtt ccgcaacgcc cgcgcgctgc atgaggcggc cccgctgatc ccgcccggtc agatgctcgt cgagaccgat gcgccctatc tcaccccgca tccgtaccgg ggtgcgccca acgagccgta ctgtctgccc tacactgtcc gggcgctggc cgatctgctc ggcagacccg ccgaggacgt ggcgtgggag accgccgaca cggccgtgag cgtgtacggt cttgacgaaa gttgccgcat ctag

Details for MythA.00318.a.B1.GE37638

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MVTSSSTRPT ERPKPPAPEP LTPLVDAHTH LDACGARDAD DVRRIMNRAG AAGVRAAVTI ADDLASARWV TRAAEWDSRV YAAVALHPTR ADALTDQARA ELEALAAHPR VVAIGETGMD LYWPGRLDGC ADPAAQREAF AWHIDLAKRT GKPLMIHNRE ADAEVLDVLR AEGAPETVIM HCFSSDAVMA RTCVEAGWVL SLSGTVSFRN ARALHEAAPL IPPGQMLVET DAPYLTPHPY RGAPNEPYCL PYTVRALADL LGRPAEDVAW ETADTAVSVY GLDESCRI

Validated NT Sequence

atcttactca cttagatgcg gcaactttcg tcaagaccgt acacgctcac ggccgtgtcg gcggtctccc acgccacgtc ctcggcgggt ctgccgagca gatcggccag cgcccggaca gtgtagggca gacagtacgg ctcgttgggc gcaccccggt acggatgcgg ggtgagatag ggcgcatcgg tctcgacgag catctgaccg ggcgggatca gcggggccgc ctcatgcagc gcgcgggcgt tgcggaacga gacggtgccg gacaggctga gcacccaccc ggcctccaca caggtccgtg ccatcaccgc gtcggacgag aagcagtgca tgatcaccgt ctccggcgcc ccctccgcgc gcagcacgtc gagcacctcg gcatcggcct cgcggttgtg gatcatcagt ggtttcccgg tgcgtttggc caggtcgatg tgccaggcga aggcctcgcg ctgggcggcc ggatcggcgc acccgtcgag gcggccgggc cagtacaggt ccataccggt ctccccgatc gccaccaccc gggggtgcgc ggccagcgcc tcgagctcgg cgcgggcctg gtcggtcagg gcgtcggcgc gggtggggtg cagcgccacc gccgcataca cccgcgaatc ccactccgcg gccctggtca cccagcgcgc cgacgccaga tcatcggcga tggtgaccgc ggcccgcacc ccggccgcac cggcgcggtt catgatccgc cgcacgtcgt cggcgtcccg ggcgccgcag gcgtcgaggt gggtgtgcgc gtcgaccagc ggggtcagcg gctcgggagc cggcggcttg ggccgttcgg tcgggcgggt ggacgagctg gtcaccatat ggtggtgggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMT SSSTRPTERP KPPAPEPLTP LVDAHTHLDA CGARDADDVR RIMNRAGAAG VRAAVTIADD LASARWVTRA AEWDSRVYAA VALHPTRADA LTDQARAELE ALAAHPRVVA IGETGMDLYW PGRLDGCADP AAQREAFAWH IDLAKRTGKP LMIHNREADA EVLDVLRAEG APETVIMHCF SSDAVMARTC VEAGWVLSLS GTVSFRNARA LHEAAPLIPP GQMLVETDAP YLTPHPYRGA PNEPYCLPYT VRALADLLGR PAEDVAWETA DTAVSVYGLD ESCRI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacca gctcgtccac ccgcccgacc gaacggccca agccgccggc tcccgagccg ctgaccccgc tggtcgacgc gcacacccac ctcgacgcct gcggcgcccg ggacgccgac gacgtgcggc ggatcatgaa ccgcgccggt gcggccgggg tgcgggccgc ggtcaccatc gccgatgatc tggcgtcggc gcgctgggtg accagggccg cggagtggga ttcgcgggtg tatgcggcgg tggcgctgca ccccacccgc gccgacgccc tgaccgacca ggcccgcgcc gagctcgagg cgctggccgc gcacccccgg gtggtggcga tcggggagac cggtatggac ctgtactggc ccggccgcct cgacgggtgc gccgatccgg ccgcccagcg cgaggccttc gcctggcaca tcgacctggc caaacgcacc gggaaaccac tgatgatcca caaccgcgag gccgatgccg aggtgctcga cgtgctgcgc gcggaggggg cgccggagac ggtgatcatg cactgcttct cgtccgacgc ggtgatggca cggacctgtg tggaggccgg gtgggtgctc agcctgtccg gcaccgtctc gttccgcaac gcccgcgcgc tgcatgaggc ggccccgctg atcccgcccg gtcagatgct cgtcgagacc gatgcgccct atctcacccc gcatccgtac cggggtgcgc ccaacgagcc gtactgtctg ccctacactg tccgggcgct ggccgatctg ctcggcagac ccgccgagga cgtggcgtgg gagaccgccg acacggccgt gagcgtgtac ggtcttgacg aaagttgccg catctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat