MyfoA.00318.a

Deoxyribonuclease (Rv1008 ortholog)

CENTER ID: MyfoA.00318.a
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.00318.a.B1.GE37895 full length 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.3123
RefSeq: WP_003884967.1
UniProt: K0V6J9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSKRSARDK PPAPEPLAPL VDAHTHLDAC GAQTAEDVRA IMDRAAAVGV GQAVTIADDL DSARWVTTAV EADDRVYGAV ALHPTRANVL DDAARAELER LAAHPRVVAV GETGMDLYWP GRLDGCATPA EQREGFAWHI DLAKRTGKPL MIHNRDADAE VLDVLRAEGA PETVIFHCFS SGPEMAHTCV EAGWVLSLSG TVSFRNAAEL REAARLIPPG QLLVETDAPF LTPHPFRGAP NEPYCLPYTV RALAELLDRP AEEVASETAA TAARVYGLNG SCPV
NT Sequence
gtgggttcca aacgctcagc cagggacaag ccgccggctc cggagccgtt ggctccgctg gtcgacgcgc acacgcacct cgacgcgtgc ggtgcgcaga ccgctgaaga tgtccgcgcg atcatggatc gggcggctgc tgtcggagtg ggccaggcgg tcaccatcgc tgatgacctg gactcggccc gttgggtgac caccgccgtc gaggccgatg accgggtgta cggcgcggtg gcgctgcacc ctacccgggc caatgtgctc gacgacgcgg ccagggcgga gctggagcgg ctggccgccc atccgcgcgt ggtggcggtc ggggagaccg gcatggacct gtactggccg ggtcgcctgg acggctgcgc gacgccggcc gagcagcggg agggtttcgc ctggcacatc gacctggcca agcgcaccgg caagccgctg atgatccaca accgggacgc cgacgccgag gtactcgacg tactgcgcgc cgaaggggcg cccgagacag tgatcttcca ctgtttttcc tctggaccgg agatggctca cacctgcgtg gaggcaggct gggtgctaag cctgtccggg acggtgagtt tccgcaacgc tgctgagctg cgcgaggccg cccggctgat cccgccgggc cagctgctgg tggagaccga tgcgccgttt ctgacgccgc atccgttccg cggagcgccg aatgagccgt attgcctgcc atacactgtg cgggcactcg cagagttgct ggaccggcct gccgaagagg tcgcgtccga gaccgcagcc actgcggcgc gggtgtacgg acttaacggg agttgcccgg tgtag
Details for MyfoA.00318.a.B1.GE37895
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 31kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV GSKRSARDKP PAPEPLAPLV DAHTHLDACG AQTAEDVRAI MDRAAAVGVG QAVTIADDLD SARWVTTAVE ADDRVYGAVA LHPTRANVLD DAARAELERL AAHPRVVAVG ETGMDLYWPG RLDGCATPAE QREGFAWHID LAKRTGKPLM IHNRDADAEV LDVLRAEGAP ETVIFHCFSS GPEMAHTCVE AGWVLSLSGT VSFRNAAELR EAARLIPPGQ LLVETDAPFL TPHPFRGAPN EPYCLPYTVR ALAELLDRPA EEVASETAAT AARVYGLNGS CPV
Validated NT Sequence
tagcannngg atcctatctt actcacttac accgggcaac tcccgttaag tccgtacacc cgcgccgcag tggctgcggt ctcggacgcg acctcttcgg caggccggtc cagcaactct gcgagtgccc gcacagtgta tggcaggcaa tacggctcat tcggcgctcc gcggaacgga tgcggcgtca gaaacggcgc atcggtctcc accagcagct ggcccggcgg gatcagccgg gcggcctcgc gcagctcagc agcgttgcgg aaactcaccg tcccggacag gcttagcacc cagcctgcct ccacgcaggt gtgagccatc tccggtccag aggaaaaaca gtggaagatc actgtctcgg gcgccccttc ggcgcgcagt acgtcgagta cctcggcgtc ggcgtcccgg ttgtggatca tcagcggctt gccggtgcgc ttggccaggt cgatgtgcca ggcgaaaccc tcccgctgct cggccggcgt cgcgcagccg tccaggcgac ccggccagta caggtccatg ccggtctccc cgaccgccac cacgcgcgga tgggcggcca gccgctccag ctccgccctg gccgcgtcgt cgagcacatt ggcccgggta gggtgcagcg ccaccgcgcc gtacacccgg tcatcggcct cgacggcggt ggtcacccaa cgggccgagt ccaggtcatc agcgatggtg accgcctggc ccactccgac agcagccgcc cgatccatga tcgcgcggac atcttcagcg gtctgcgcac cgcacgcgtc gaggtgcgtg tgcgcgtcga ccagcggagc caacggctcc ggagccggcg gcttgtccct ggctgagcgt ttggaaccca ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMG SKRSARDKPP APEPLAPLVD AHTHLDACGA QTAEDVRAIM DRAAAVGVGQ AVTIADDLDS ARWVTTAVEA DDRVYGAVAL HPTRANVLDD AARAELERLA AHPRVVAVGE TGMDLYWPGR LDGCATPAEQ REGFAWHIDL AKRTGKPLMI HNRDADAEVL DVLRAEGAPE TVIFHCFSSG PEMAHTCVEA GWVLSLSGTV SFRNAAELRE AARLIPPGQL LVETDAPFLT PHPFRGAPNE PYCLPYTVRA LAELLDRPAE EVASETAATA ARVYGLNGSC PV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgggtt ccaaacgctc agccagggac aagccgccgg ctccggagcc gttggctccg ctggtcgacg cgcacacgca cctcgacgcg tgcggtgcgc agaccgctga agatgtccgc gcgatcatgg atcgggcggc tgctgtcgga gtgggccagg cggtcaccat cgctgatgac ctggactcgg cccgttgggt gaccaccgcc gtcgaggccg atgaccgggt gtacggcgcg gtggcgctgc accctacccg ggccaatgtg ctcgacgacg cggccagggc ggagctggag cggctggccg cccatccgcg cgtggtggcg gtcggggaga ccggcatgga cctgtactgg ccgggtcgcc tggacggctg cgcgacgccg gccgagcagc gggagggttt cgcctggcac atcgacctgg ccaagcgcac cggcaagccg ctgatgatcc acaaccggga cgccgacgcc gaggtactcg acgtactgcg cgccgaaggg gcgcccgaga cagtgatctt ccactgtttt tcctctggac cggagatggc tcacacctgc gtggaggcag gctgggtgct aagcctgtcc gggacggtga gtttccgcaa cgctgctgag ctgcgcgagg ccgcccggct gatcccgccg ggccagctgc tggtggagac cgatgcgccg tttctgacgc cgcatccgtt ccgcggagcg ccgaatgagc cgtattgcct gccatacact gtgcgggcac tcgcagagtt gctggaccgg cctgccgaag aggtcgcgtc cgagaccgca gccactgcgg cgcgggtgta cggacttaac gggagttgcc cggtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat