MyleA.00318.a

Putative uncharacterized protein (Rv1008 ortholog)

CENTER ID: MyleA.00318.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00318.a.B1.GE37457 full length 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.369
RefSeq: YP_002502929.1
UniProt: A0A0H3MTW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVDAHTHLDA CGARDADDVN AIVERAAAVG VVAVVTVADD LESARWVIAA AGWDRRVYAA VALHPTRANS LTSAARAKIE QLAAHPRMVA VGETGMDLYW PGRLDSCAPP DVQREAFAWH IDLAKRCGKP LMIHNRQADA EVLDVLRAEG APEQVIFHCF SSNAAMARRC VDAGWMLSLS GTVSFRNARP LREAVPLIPA QQLLVETDAP FLTPHPHRGL ANEPYCLPYT VRAVAELVER SPEELAQITT SNALRVYKLG SV
NT Sequence
ttggtcgatg cccataccca cctcgacgcg tgcggcgccc gcgacgccga tgacgtgaat gccatcgttg agcgagccgc ggctgtaggt gtggttgcgg tcgtcaccgt cgcagacgac ttggaatctg cgcgttgggt cattgctgcc gccgggtggg accggcgggt gtacgcggcg gtggcgttgc acccgacgcg agcaaactcg ctcaccagtg ccgctcgggc caagattgaa caactggcgg cccatccgcg gatggtggct gtcggcgaga ccggtatgga tttgtattgg cccggccgcc tcgacagctg tgccccacct gacgtgcagc gcgaggcgtt cgcatggcat attgatcttg ccaagcgatg tggcaaaccg ctgatgatcc acaaccggca agctgacgcg gaagtgcttg acgtcttgcg tgctgagggc gcccccgagc aagtaatttt tcattgtttt tcgtctaatg ctgcgatggc ccgccgctgc gtagacgccg ggtggatgct cagcttgtcc gggacggtga gtttccgtaa tgctcgtccc ctgcgggaag ctgttccgtt gatcccagcg caacagcttc tggtggaaac cgatgcgcca tttttgaccc cgcatcccca ccggggtcta gctaacgaac cttactgcct tccttacact gttagagccg ttgctgaatt ggttgaacgt tccccagaag agctggcgca aatcaccact agcaatgctc tacgcgtgta caagctagga tctgtatag
Details for MyleA.00318.a.B1.GE37457
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML VDAHTHLDAC GARDADDVNA IVERAAAVGV VAVVTVADDL ESARWVIAAA GWDRRVYAAV ALHPTRANSL TSAARAKIEQ LAAHPRMVAV GETGMDLYWP GRLDSCAPPD VQREAFAWHI DLAKRCGKPL MIHNRQADAE VLDVLRAEGA PEQVIFHCFS SNAAMARRCV DAGWMLSLSG TVSFRNARPL REAVPLIPAQ QLLVETDAPF LTPHPHRGLA NEPYCLPYTV RAVAELVERS PEELAQITTS NALRVYKLGS V
Validated NT Sequence
atcttactca cttatacaga tcctagcttg tacacgcgta gagcattgct agtggtgatt tgcgccagct cttctgggga acgttcaacc aattcagcaa cggctctaac agtgtaagga aggcagtaag gttcgttagc tagaccccgg tggggatgcg gggtcaaaaa tggcgcatcg gtttccacca gaagctgttg cgctgggatc aacggaacag cttcccgcag gggacgagca ttacggaaac tcaccgtccc ggacaagctg agcatccacc cggcgtctac gcagcggcgg gccatcgcag cattagacga aaaacaatga aaaattactt gctcgggggc gccctcagca cgcaagacgt caagcacttc cgcgtcagct tgccggttgt ggatcatcag cggtttgcca catcgcttgg caagatcaat atgccatgcg aacgcctcgc gctgcacgtc aggtggggca cagctgtcga ggcggccggg ccaatacaaa tccataccgg tctcgccgac agccaccatc cgcggatggg ccgccagttg ttcaatcttg gcccgagcgg cactggtgag cgagtttgct cgcgtcgggt gcaacgccac cgccgcgtac acccgccggt cccacccggc ggcagcaatg acccaacgcg cagattccaa gtcgtctgcg acggtgacga ccgcaaccac acctacagcc gcggctcgct caacgatggc attcacgtca tcggcgtcgc gggcgccgca cgcgtcgagg tgggtatggg catcgaccaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMV DAHTHLDACG ARDADDVNAI VERAAAVGVV AVVTVADDLE SARWVIAAAG WDRRVYAAVA LHPTRANSLT SAARAKIEQL AAHPRMVAVG ETGMDLYWPG RLDSCAPPDV QREAFAWHID LAKRCGKPLM IHNRQADAEV LDVLRAEGAP EQVIFHCFSS NAAMARRCVD AGWMLSLSGT VSFRNARPLR EAVPLIPAQQ LLVETDAPFL TPHPHRGLAN EPYCLPYTVR AVAELVERSP EELAQITTSN ALRVYKLGSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggtcg atgcccatac ccacctcgac gcgtgcggcg cccgcgacgc cgatgacgtg aatgccatcg ttgagcgagc cgcggctgta ggtgtggttg cggtcgtcac cgtcgcagac gacttggaat ctgcgcgttg ggtcattgct gccgccgggt gggaccggcg ggtgtacgcg gcggtggcgt tgcacccgac gcgagcaaac tcgctcacca gtgccgctcg ggccaagatt gaacaactgg cggcccatcc gcggatggtg gctgtcggcg agaccggtat ggatttgtat tggcccggcc gcctcgacag ctgtgcccca cctgacgtgc agcgcgaggc gttcgcatgg catattgatc ttgccaagcg atgtggcaaa ccgctgatga tccacaaccg gcaagctgac gcggaagtgc ttgacgtctt gcgtgctgag ggcgcccccg agcaagtaat ttttcattgt ttttcgtcta atgctgcgat ggcccgccgc tgcgtagacg ccgggtggat gctcagcttg tccgggacgg tgagtttccg taatgctcgt cccctgcggg aagctgttcc gttgatccca gcgcaacagc ttctggtgga aaccgatgcg ccatttttga ccccgcatcc ccaccggggt ctagctaacg aaccttactg ccttccttac actgttagag ccgttgctga attggttgaa cgttccccag aagagctggc gcaaatcacc actagcaatg ctctacgcgt gtacaagcta ggatctgtat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat