MyavA.00318.a

Hydrolase, TatD family protein (Rv1008 ortholog)

CENTER ID: MyavA.00318.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00318.a.B1.GE37402 full length 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1119
RefSeq: YP_880396.1
UniProt: A0A0H2ZVZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVSSNRRER QAPPAPEPLA PLIDAHTHLD ACAAGPETGA ADVRAIVDRA AAVGVQAVVT VADDLDSARW VTRAAEWDPR VYAATALHPT RADALDDAAR AEIERLVAHP RVVAVGETGM DLYWPGRLDG CAEPAVQREA FAWHIDLAKR CGKPLMIHNR EADAEVLDVL AAEGAPDVVI FHCFSSDAAM ARRCVDAGWL LSLSGTVSFR NARALREAVP LIPPGQLLVE TDAPFLTPHP HRGTANEPYC LPYTVRAIAE LVDRRPEELA AVTTDNARRV YGLA
NT Sequence
gtgcgcgtga gctccaaccg acgagaacga caagccccgc ccgccccgga gccgctggcg cccctgatcg acgcccacac ccacctcgac gcctgcgccg ccggaccgga gacgggagcc gccgacgtgc gggccatcgt ggaccgcgcg gcggcggtgg gggtgcaggc ggtggtcacc gtcgccgacg acctggactc ggcgcgctgg gtcacccgcg cggcggagtg ggatccgcgg gtgtacgcgg ccacggcgct gcatcccacc cgcgccgacg cgctggacga cgcggcgcgc gccgagatcg agcggctggt ggcccacccc cgggtggtgg cggtcgggga gaccggcatg gacctgtact ggccggggcg gctggacggc tgcgccgagc cggccgtgca gcgcgaggcc ttcgcctggc atatcgatct ggccaaacgg tgcgggaaac cgctgatgat ccacaaccgg gaggccgacg ccgaggtgct cgacgtgctg gccgccgagg gtgccccgga cgtggtgatc ttccactgct tctcgtcgga tgcggccatg gcccgccgct gcgtggacgc cggttggctg ctgagcctgt ccggcaccgt gagcttccgc aacgcccgcg ccctgcgcga agccgtgccg ttgataccgc ccgggcagct gctggtggaa acggatgcgc cgtttttgac cccgcacccg caccggggga cggccaatga gccctactgc cttccttata ctgttcgggc gatcgccgaa ctggtggatc gccgccccga agagctggcc gcggtgacca ccgacaatgc tcgacgggtg tacggactgg cctga
Details for MyavA.00318.a.B1.GE37402
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/4/2013
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV RVSSNRRERQ APPAPEPLAP LIDAHTHLDA CAAGPETGAA DVRAIVDRAA AVGVQAVVTV ADDLDSARWV TRAAEWDPRV YAATALHPTR ADALDDAARA EIERLVAHPR VVAVGETGMD LYWPGRLDGC AEPAVQREAF AWHIDLAKRC GKPLMIHNRE ADAEVLDVLA AEGAPDVVIF HCFSSDAAMA RRCVDAGWLL SLSGTVSFRN ARALREAVPL IPPGQLLVET DAPFLTPHPH RGTANEPYCL PYTVRAIAEL VDRRPEELAA VTTDNARRVY GLA
Validated NT Sequence
ttactcactt aggccagtcc gtacacccgt cgagcattgt ccgtcgtcac cgcggccagc tcttcggggc ggcgatccac cagttcggcg atcgcccgaa cagtataagg aaggcagtag ggctcattgg ccgtcccccg gtgcgggtgc ggggtcaaaa acggcgcatc cgtttccacc agcagctgcc cgggcggtat caacggcacg gcttcgcgca gggcgcgggc gttgcggaag ctcacggtgc cggacaggct cagcagccaa ccggcgtcca cgcagcggcg ggccatggcc gcatccgacg agaagcagtg gaagatcacc acgtccgggg caccctcggc ggccagcacg tcgagcacct cggcgtcggc ctcccggttg tggatcatca gcggtttccc gcaccgtttg gccagatcga tatgccaggc gaaggcctcg cgctgcacgg ccggctcggc gcagccgtcc agccgccccg gccagtacag gtccatgccg gtctccccga ccgccaccac ccgggggtgg gccaccagcc gctcgatctc ggcgcgcgcc gcgtcgtcca gcgcgtcggc gcgggtggga tgcagcgccg tggccgcgta cacccgcgga tcccactccg ccgcgcgggt gacccagcgc gccgagtcca ggtcgtcggc gacggtgacc accgcctgca cccccaccgc cgccgcgcgg tccacgatgg cccgcacgtc ggcggctccc gtctccggtc cggcggcgca ggcgtcgagg tgggtgtggg cgtcgatcag gggcgccagc ggctccgggg cgggcggggc ttgtcgttct cgtcggttgg agctcacgcg caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMR VSSNRRERQA PPAPEPLAPL IDAHTHLDAC AAGPETGAAD VRAIVDRAAA VGVQAVVTVA DDLDSARWVT RAAEWDPRVY AATALHPTRA DALDDAARAE IERLVAHPRV VAVGETGMDL YWPGRLDGCA EPAVQREAFA WHIDLAKRCG KPLMIHNREA DAEVLDVLAA EGAPDVVIFH CFSSDAAMAR RCVDAGWLLS LSGTVSFRNA RALREAVPLI PPGQLLVETD APFLTPHPHR GTANEPYCLP YTVRAIAELV DRRPEELAAV TTDNARRVYG LA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcg tgagctccaa ccgacgagaa cgacaagccc cgcccgcccc ggagccgctg gcgcccctga tcgacgccca cacccacctc gacgcctgcg ccgccggacc ggagacggga gccgccgacg tgcgggccat cgtggaccgc gcggcggcgg tgggggtgca ggcggtggtc accgtcgccg acgacctgga ctcggcgcgc tgggtcaccc gcgcggcgga gtgggatccg cgggtgtacg cggccacggc gctgcatccc acccgcgccg acgcgctgga cgacgcggcg cgcgccgaga tcgagcggct ggtggcccac ccccgggtgg tggcggtcgg ggagaccggc atggacctgt actggccggg gcggctggac ggctgcgccg agccggccgt gcagcgcgag gccttcgcct ggcatatcga tctggccaaa cggtgcggga aaccgctgat gatccacaac cgggaggccg acgccgaggt gctcgacgtg ctggccgccg agggtgcccc ggacgtggtg atcttccact gcttctcgtc ggatgcggcc atggcccgcc gctgcgtgga cgccggttgg ctgctgagcc tgtccggcac cgtgagcttc cgcaacgccc gcgccctgcg cgaagccgtg ccgttgatac cgcccgggca gctgctggtg gaaacggatg cgccgttttt gaccccgcac ccgcaccggg ggacggccaa tgagccctac tgccttcctt atactgttcg ggcgatcgcc gaactggtgg atcgccgccc cgaagagctg gccgcggtga ccaccgacaa tgctcgacgg gtgtacggac tggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat