MymaA.00408.a

Deoxyribonuclease TatD (Rv1008 ortholog)

CENTER ID: MymaA.00408.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00408.a.B1.GE37188 full length 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4814
RefSeq: YP_001852741.1
UniProt: B2HDK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVSSNRAAE REAPPAPQPL APLIDAHTHL DACGAADAAG VIAIVDRAAA VGVEAVVTIA DDLESARWVT RAAEWDPRVY AAVALHPTRT DALTEAARRE IEELVAHRRV VAVGETGMDM YWPGRLEGCA DPDVQREAFA WHIDLAKRTG KPLMIHNRQA DREVLDVLRA EGAPDTVIFH CFSSDAAMAR ECVAAGWLLS LSGTVSFRNA RELREAVPLI PLDQLLVETD APFLTPHPYR GAANEPYCLP YTVRALAELL NGSAEELAQV TASNARRVYG LG
NT Sequence
gtgcgcgtga gctccaaccg cgccgccgaa cgagaagcac cgcccgcccc gcagcccctg gcaccgttga tcgacgcgca tacccatctc gatgcctgcg gcgccgccga tgccgcggga gtgatcgcca tcgtcgaccg tgccgcggcg gtcggggtgg aggcggtggt gaccatcgct gacgatctgg aatcggcgcg gtgggtgacc cgtgcggccg agtgggatcc gcgggtctac gccgcggtcg cgctgcaccc gacccggacc gacgcgctga ccgaggccgc gcgccgtgaa atcgaagaac tggtagctca tcggcgggta gtggcggtgg gggagaccgg tatggacatg tattggccgg gtcgactgga aggatgcgcg gaccccgacg tgcagcggga ggcttttgcc tggcacatcg acctggccaa gcggaccggc aagccgctga tgatccacaa ccggcaggcc gatcgcgagg tgctcgacgt gctgcgcgcc gagggcgctc ccgacaccgt gatcttccac tgcttctcgt cggacgctgc gatggcccgc gagtgtgtgg cggccggatg gttgctgagc ctgtccggga cggtgagctt ccgcaacgcc cgcgagctgc gcgaagcggt cccgctgata ccgctggacc agcttctggt cgaaaccgat gcaccttttt tgaccccgca cccctaccgc ggtgcagcga acgaaccgta ttgcctgccc tacaccgttc gggcactcgc tgagctgctc aatggtagtg ctgaggaatt ggcccaggta acggccagca atgctcggcg agtgtatgga ctaggctag
Details for MymaA.00408.a.B1.GE37188
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 32kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MVRVSSNRAA EREAPPAPQP LAPLIDAHTH LDACGAADAA GVIAIVDRAA AVGVEAVVTI ADDLESARWV TRAAEWDPRV YAAVALHPTR TDALTEAARR EIEELVAHRR VVAVGETGMD MYWPGRLEGC ADPDVQREAF AWHIDLAKRT GKPLMIHNRQ ADREVLDVLR AEGAPDTVIF HCFSSDAAMA RECVAAGWLL SLSGTVSFRN ARELREAVPL IPLDQLLVET DAPFLTPHPY RGAANEPYCL PYTVRALAEL LNGSAEELAQ VTASNARRVY GLG
Validated NT Sequence
atcttactca cttagcctag tccatacact cgccgagcat tgctggccgt tacctgggcc aattcctcag cactaccatt gagcagctca gcgagtgccc gaacggtgta gggcaggcaa tacggttcgt tcgctgcacc gcggtagggg tgcggggtca aaaaaggtgc atcggtttcg accagaagct ggtccagcgg tatcagcggg accgcttcgc gcagctcgcg ggcgttgcgg aagctcaccg tcccggacag gctcagcaac catccggccg ccacacactc gcgggccatc gcagcgtccg acgagaagca gtggaagatc acggtgtcgg gagcgccctc ggcgcgcagc acgtcgagca cctcgcgatc ggcctgccgg ttgtggatca tcagcggctt gccggtccgc ttggccaggt cgatgtgcca ggcaaaagcc tcccgctgca cgtcggggtc cgcgcatcct tccagtcgac ccggccaata catgtccata ccggtctccc ccaccgccac tacccgccga tgagctacca gttcttcgat ttcacggcgc gcggcctcgg tcagcgcgtc ggtccgggtc gggtgcagcg cgaccgcggc gtagacccgc ggatcccact cggccgcacg ggtcacccac cgcgccgatt ccagatcgtc agcgatggtc accaccgcct ccaccccgac cgccgcggca cggtcgacga tggcgatcac tcccgcggca tcggcggcgc cgcaggcatc gagatgggta tgcgcgtcga tcaacggtgc caggggctgc ggggcgggcg gtgcttctcg ttcggcggcg cggttggagc tcacgcgcac catatggtgg tgg
Expected Protein Sequence
MAHHHHHHMR VSSNRAAERE APPAPQPLAP LIDAHTHLDA CGAADAAGVI AIVDRAAAVG VEAVVTIADD LESARWVTRA AEWDPRVYAA VALHPTRTDA LTEAARREIE ELVAHRRVVA VGETGMDMYW PGRLEGCADP DVQREAFAWH IDLAKRTGKP LMIHNRQADR EVLDVLRAEG APDTVIFHCF SSDAAMAREC VAAGWLLSLS GTVSFRNARE LREAVPLIPL DQLLVETDAP FLTPHPYRGA ANEPYCLPYT VRALAELLNG SAEELAQVTA SNARRVYGLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcg tgagctccaa ccgcgccgcc gaacgagaag caccgcccgc cccgcagccc ctggcaccgt tgatcgacgc gcatacccat ctcgatgcct gcggcgccgc cgatgccgcg ggagtgatcg ccatcgtcga ccgtgccgcg gcggtcgggg tggaggcggt ggtgaccatc gctgacgatc tggaatcggc gcggtgggtg acccgtgcgg ccgagtggga tccgcgggtc tacgccgcgg tcgcgctgca cccgacccgg accgacgcgc tgaccgaggc cgcgcgccgt gaaatcgaag aactggtagc tcatcggcgg gtagtggcgg tgggggagac cggtatggac atgtattggc cgggtcgact ggaaggatgc gcggaccccg acgtgcagcg ggaggctttt gcctggcaca tcgacctggc caagcggacc ggcaagccgc tgatgatcca caaccggcag gccgatcgcg aggtgctcga cgtgctgcgc gccgagggcg ctcccgacac cgtgatcttc cactgcttct cgtcggacgc tgcgatggcc cgcgagtgtg tggcggccgg atggttgctg agcctgtccg ggacggtgag cttccgcaac gcccgcgagc tgcgcgaagc ggtcccgctg ataccgctgg accagcttct ggtcgaaacc gatgcacctt ttttgacccc gcacccctac cgcggtgcag cgaacgaacc gtattgcctg ccctacaccg ttcgggcact cgctgagctg ctcaatggta gtgctgagga attggcccag gtaacggcca gcaatgctcg gcgagtgtat ggactaggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat