MysmA.00414.b

3-oxoacyl-[acyl-carrier-protein] reductase

CENTER ID: MysmA.00414.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00414.b.A1.GE28635 Full length( MysmA.00414.b ) 1 251
MysmA.00414.b.AE1.GE43159 Full length( MysmA.00414.b ) 1 251
MysmA.00414.b.B1.GE43081 Full length( MysmA.00414.b ) 1 251
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
8CXA
DEPOSITED: 5/20/2022
DETERMINATION: XRay
CLONE: MysmA.00414.b.A1.GE28635
PROTEIN: MysmA.00414.b.A1.PW28534
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126618
PATRIC ID: fig|246196.19.peg.2502
RefSeq: YP_886873.1
UniProt: A0QVD5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLANRVAII TGAAQGIGRI YAERFAEEGA AVVVADINEP KATEVASSIT SLGGRAIAAA VDVSDVESVN RMVDSAVEAF GTVDILVNNA AIFSTLKMKP FEEISGDEWD KLFAVNAKGT FLCCQAVSPV MRKNQYGRII NISSSVVVTG RPNYAHYIAS KGAVWALTHA LATEMGTDGI TVNTVSPHGI ITEIPRETIS EEGWRRNLEE QALKRKGDAS DLVGILLYLA SDESKFMTGQ TVALDAGLRF T
NT Sequence
GTGAGACTTG CAAACAGAGT CGCCATCATC ACCGGGGCAG CACAGGGTAT CGGTCGGATC TATGCCGAGA GATTCGCCGA GGAAGGTGCG GCTGTGGTGG TCGCAGACAT CAATGAGCCC AAAGCGACCG AGGTCGCATC ATCCATCACG TCGCTGGGCG GCCGCGCAAT CGCCGCTGCC GTCGATGTCT CGGATGTCGA ATCGGTCAAT CGTATGGTCG ATTCCGCCGT GGAGGCGTTC GGAACCGTCG ACATCCTGGT CAACAACGCC GCGATCTTCT CCACGCTGAA GATGAAGCCT TTCGAGGAGA TCTCGGGCGA CGAGTGGGAC AAGCTCTTTG CGGTGAACGC CAAGGGGACG TTCCTGTGCT GTCAGGCTGT TTCGCCGGTG ATGCGCAAGA ACCAGTACGG CCGCATCATC AACATCTCGT CGAGCGTCGT GGTGACGGGC CGGCCCAACT ATGCCCACTA CATCGCGTCC AAAGGCGCCG TCTGGGCGTT GACCCACGCT CTGGCAACGG AAATGGGCAC CGACGGCATC ACGGTCAACA CCGTGAGCCC GCACGGCATC ATCACCGAAA TCCCGCGGGA GACCATCTCG GAAGAGGGGT GGAGGCGAAA TCTGGAAGAG CAGGCACTCA AGCGCAAGGG TGATGCCTCC GACCTGGTCG GAATCCTGCT CTACCTCGCC AGTGACGAAA GCAAGTTCAT GACCGGCCAG ACCGTCGCCC TGGACGCCGG TTTGCGCTTC ACC
Details for MysmA.00414.b.A1.GE28635
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRLANRVA IITGAAQGIG RIYAERFAEE GAAVVVADIN EPKATEVASS ITSLGGRAIA AAVDVSDVES VNRMVDSAVE AFGTVDILVN NAAIFSTLKM KPFEEISGDE WDKLFAVNAK GTFLCCQAVS PVMRKNQYGR IINISSSVVV TGRPNYAHYI ASKGAVWALT HALATEMGTD GITVNTVSPH GIITEIPRET ISEEGWRRNL EEQALKRKGD ASDLVGILLY LASDESKFMT GQTVALDAGL RFT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gacttgcaaa cagagtcgcc atcatcaccg gggcagcaca gggtatcggt cggatctatg ccgagagatt cgccgaggaa ggtgcggctg tggtggtcgc agacatcaat gagcccaaag cgaccgaggt cgcatcatcc atcacgtcgc tgggcggccg cgcaatcgcc gctgccgtcg atgtctcgga tgtcgaatcg gtcaatcgta tggtcgattc cgccgtggag gcgttcggaa ccgtcgacat cctggtcaac aacgccgcga tcttctccac gctgaagatg aagcctttcg aggagatctc gggcgacgag tgggacaagc tctttgcggt gaacgccaag gggacgttcc tgtgctgtca ggctgtttcg ccggtgatgc gcaagaacca gtacggccgc atcatcaaca tctcgtcgag cgtcgtggtg acgggccggc ccaactatgc ccactacatc gcgtccaaag gcgccgtctg ggcgttgacc cacgctctgg caacggaaat gggcaccgac ggcatcacgg tcaacaccgt gagcccgcac ggcatcatca ccgaaatccc gcgggagacc atctcggaag aggggtggag gcgaaatctg gaagagcagg cactcaagcg caagggtgat gcctccgacc tggtcggaat cctgctctac ctcgccagtg acgaaagcaa gttcatgacc ggccagaccg tcgccctgga cgccggtttg cgcttcacct aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRLANRVA IITGAAQGIG RIYAERFAEE GAAVVVADIN EPKATEVASS ITSLGGRAIA AAVDVSDVES VNRMVDSAVE AFGTVDILVN NAAIFSTLKM KPFEEISGDE WDKLFAVNAK GTFLCCQAVS PVMRKNQYGR IINISSSVVV TGRPNYAHYI ASKGAVWALT HALATEMGTD GITVNTVSPH GIITEIPRET ISEEGWRRNL EEQALKRKGD ASDLVGILLY LASDESKFMT GQTVALDAGL RFT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagactt gcaaacagag tcgccatcat caccggggca gcacagggta tcggtcggat ctatgccgag agattcgccg aggaaggtgc ggctgtggtg gtcgcagaca tcaatgagcc caaagcgacc gaggtcgcat catccatcac gtcgctgggc ggccgcgcaa tcgccgctgc cgtcgatgtc tcggatgtcg aatcggtcaa tcgtatggtc gattccgccg tggaggcgtt cggaaccgtc gacatcctgg tcaacaacgc cgcgatcttc tccacgctga agatgaagcc tttcgaggag atctcgggcg acgagtggga caagctcttt gcggtgaacg ccaaggggac gttcctgtgc tgtcaggctg tttcgccggt gatgcgcaag aaccagtacg gccgcatcat caacatctcg tcgagcgtcg tggtgacggg ccggcccaac tatgcccact acatcgcgtc caaaggcgcc gtctgggcgt tgacccacgc tctggcaacg gaaatgggca ccgacggcat cacggtcaac accgtgagcc cgcacggcat catcaccgaa atcccgcggg agaccatctc ggaagagggg tggaggcgaa atctggaaga gcaggcactc aagcgcaagg gtgatgcctc cgacctggtc ggaatcctgc tctacctcgc cagtgacgaa agcaagttca tgaccggcca gaccgtcgcc ctggacgccg gtttgcgctt caccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00414.b.AE1.GE43159
HARVESTED ON: data unavailable
SEQUENCED ON: 5/24/2019
EXPECTED MW: 28kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 84
Validated AA Sequence
MXRLAXXVAI ITGAAQGIGR IYAERFAEEG AAVVVADINE PKATEVASSI TSLGGRAIAA AVDVSDVESV NRMVDSAVEA FGTVDILVNN AAIFSTLKMK PFEEISGDEW DKLFAVNAKG TFLCCQAVSP VMRKNQYGRI INISSSVVVT GRPNYAHYIA SKXAVWALTH ALATEMGXXG ITVNXREPAR HHHRNPAGDH LGRGVEAKSG RAGTQAQG
Validated NT Sequence
gnnnnnnntg gtggtgccca gnnnnnnngc gcaaaccggc gtccagggcg acggtctggc cggtcatgaa cttgctttcg tcactggcga gnnnnngnag gattccgacc annnnnnnng gcatcaccct tgcgcttgag tgcctgctct tccagatttc gcctccaccc ctcttccgag atggtctccc gcgggatttc ggtgatgatg ccgtgcgggc tcacgnnngt tgaccgtgat gccgtnnntg cccatttccg ttgccagagc gtgggtcaac gcccagacgg cnnctttgga cgcgatgtag tgggcatagt tgggccggcc cgtcaccacg acgctcgacg agatgttgat gatgcggccg tactggttct tgcgcatcac cggcgaaaca gcctgacagc acaggaacgt ccccttggcg ttcaccgcaa agagcttgtc ccactcgtcg cccgagatct cctcgaaagg cttcatcttc agcgtggaga agatcgcggc gttgttgacc aggatgtcga cggttccgaa cgcctccacg gcggaatcga ccatacgatt gaccgattcg acatccgaga catcgacggc agcggcgatt gcgcggccgc ccagcgacgt gatggatgat gcgacctcgg tcgctttggg ctcattgatg tctgcgacca ccacagccgc accttcctcg gcgaatctct cggcatagat ccgaccgata ccctgtgctg ccccggtgat gatggcgacn nnnnnngcaa gtctnnncat gggagaga
Expected Protein Sequence
MRLANRVAII TGAAQGIGRI YAERFAEEGA AVVVADINEP KATEVASSIT SLGGRAIAAA VDVSDVESVN RMVDSAVEAF GTVDILVNNA AIFSTLKMKP FEEISGDEWD KLFAVNAKGT FLCCQAVSPV MRKNQYGRII NISSSVVVTG RPNYAHYIAS KGAVWALTHA LATEMGTDGI TVNTVSPHGI ITEIPRETIS EEGWRRNLEE QALKRKGDAS DLVGILLYLA SDESKFMTGQ TVALDAGLRF TGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gagacttgca aacagagtcg ccatcatcac cggggcagca cagggtatcg gtcggatcta tgccgagaga ttcgccgagg aaggtgcggc tgtggtggtc gcagacatca atgagcccaa agcgaccgag gtcgcatcat ccatcacgtc gctgggcggc cgcgcaatcg ccgctgccgt cgatgtctcg gatgtcgaat cggtcaatcg tatggtcgat tccgccgtgg aggcgttcgg aaccgtcgac atcctggtca acaacgccgc gatcttctcc acgctgaaga tgaagccttt cgaggagatc tcgggcgacg agtgggacaa gctctttgcg gtgaacgcca aggggacgtt cctgtgctgt caggctgttt cgccggtgat gcgcaagaac cagtacggcc gcatcatcaa catctcgtcg agcgtcgtgg tgacgggccg gcccaactat gcccactaca tcgcgtccaa aggcgccgtc tgggcgttga cccacgctct ggcaacggaa atgggcaccg acggcatcac ggtcaacacc gtgagcccgc acggcatcat caccgaaatc ccgcgggaga ccatctcgga agaggggtgg aggcgaaatc tggaagagca ggcactcaag cgcaagggtg atgcctccga cctggtcgga atcctgctct acctcgccag tgacgaaagc aagttcatga ccggccagac cgtcgccctg gacgccggtt tgcgcttcac cgggcaccac catcatcatc attaacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for MysmA.00414.b.B1.GE43081
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMV RLANRVAIIT GAAQGIGRIY AERFAEEGAA VVVADINEPK ATEVASSITS LGGRAIAAAV DVSDVESVNR MVDSAVEAFG TVDILVNNAA IFSTLKMKPF EEISGDEWDK LFAVNAKGTF LCCQAVSPVM RKNQYGRIIN ISSSVVVTGR PNYAHYIASK GAVWALTHAL ATEMGTDGIT VNTVSPHGII TEIPRETISE EGWRRNLEEQ ALKRKGDASD LVGILLXLAS DESKFMTGQT V
Validated NT Sequence
ggcgacggtc tggccggtca tgaacttgct ttcgtcactg gcgaggtnga gcaggattcc gaccaggtcg gaggcatcac ccttgcgctt gagtgcctgc tcttccagat ttcgcctcca cccctcttcc gagatggtct cccgcgggat ttcggtgatg atgccgtgcg ggctcacggt gttgaccgtg atgccgtcgg tgcccatttc cgttgccaga gcgtgggtca acgcccagac ggcgcctttg gacgcgatgt agtgggcata gttgggccgg cccgtcacca cgacgctcga cgagatgttg atgatgcggc cgtactggtt cttgcgcatc accggcgaaa cagcctgaca gcacaggaac gtccccttgg cgttcaccgc aaagagcttg tcccactcgt cgcccgagat ctcctcgaaa ggcttcatct tcagcgtgga gaagatcgcg gcgttgttga ccaggatgtc gacggttccg aacgcctcca cggcggaatc gaccatacga ttgaccgatt cgacatccga gacatcgacg gcagcggcga ttgcgcggcc gcccagcgac gtgatggatg atgcgacctc ggtcgctttg ggctcattga tgtctgcgac caccacagcc gcaccttcct cggcgaatct ctcggcatag atccgaccga taccctgtgc tgccccggtg atgatggcga ctctgtttgc aagtctcacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMR LANRVAIITG AAQGIGRIYA ERFAEEGAAV VVADINEPKA TEVASSITSL GGRAIAAAVD VSDVESVNRM VDSAVEAFGT VDILVNNAAI FSTLKMKPFE EISGDEWDKL FAVNAKGTFL CCQAVSPVMR KNQYGRIINI SSSVVVTGRP NYAHYIASKG AVWALTHALA TEMGTDGITV NTVSPHGIIT EIPRETISEE GWRRNLEEQA LKRKGDASDL VGILLYLASD ESKFMTGQTV ALDAGLRFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagac ttgcaaacag agtcgccatc atcaccgggg cagcacaggg tatcggtcgg atctatgccg agagattcgc cgaggaaggt gcggctgtgg tggtcgcaga catcaatgag cccaaagcga ccgaggtcgc atcatccatc acgtcgctgg gcggccgcgc aatcgccgct gccgtcgatg tctcggatgt cgaatcggtc aatcgtatgg tcgattccgc cgtggaggcg ttcggaaccg tcgacatcct ggtcaacaac gccgcgatct tctccacgct gaagatgaag cctttcgagg agatctcggg cgacgagtgg gacaagctct ttgcggtgaa cgccaagggg acgttcctgt gctgtcaggc tgtttcgccg gtgatgcgca agaaccagta cggccgcatc atcaacatct cgtcgagcgt cgtggtgacg ggccggccca actatgccca ctacatcgcg tccaaaggcg ccgtctgggc gttgacccac gctctggcaa cggaaatggg caccgacggc atcacggtca acaccgtgag cccgcacggc atcatcaccg aaatcccgcg ggagaccatc tcggaagagg ggtggaggcg aaatctggaa gagcaggcac tcaagcgcaa gggtgatgcc tccgacctgg tcggaatcct gctctacctc gccagtgacg aaagcaagtt catgaccggc cagaccgtcg ccctggacgc cggtttgcgc ttcacctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t