SSGCID: Target Page

MypaA.00414.a

Putative uncharacterized protein

CENTER ID:	MypaA.00414.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00414.a.A1.GE29446	Full length( MypaA.00414.a )		1	267

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00414.a.A1.PS00729	Full length( MypaA.00414.a )		1	267

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3537
RefSeq:	NP_962264.1
UniProt:	Q73UN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRVAAVKGI SMGLRGLADK VAVVVGGATG IGAATAARLA GEGCRVVIGD VAVDAARQTA DRIAAAGGTA TQVAFDLADP ASVATLIDCA ATTYGGVDLL FNVGADMSTI RADTDVVDID FDVWDRVMTV SLRGYVAAMK YAIPRMLDRG GGAIVNMSSA AAFQGEPARP AYATAKAGIG ALTRHVASRW GKDNIRCNAV APGFTATETI RSVPQWPELE AAALKRIRGP RVGDPADVAT LVAFLLSAEG DWINGQVINI DGGTVLR

NT Sequence

TTGCGGCGGG TTGCGGCGGT GAAGGGGATT TCCATGGGTC TTCGCGGTCT GGCGGACAAG GTCGCTGTCG TGGTCGGCGG GGCCACCGGC ATCGGTGCGG CGACGGCCGC CCGGCTGGCC GGGGAAGGCT GCCGCGTGGT CATCGGCGAC GTGGCCGTCG ACGCCGCGCG GCAGACGGCC GACCGCATCG CCGCCGCCGG CGGCACGGCG ACCCAGGTGG CCTTCGACCT CGCCGACCCG GCGTCGGTTG CCACCCTGAT CGACTGTGCC GCAACGACTT ACGGCGGCGT GGACCTGCTG TTCAACGTGG GCGCCGACAT GAGCACCATC CGGGCCGACA CCGACGTGGT CGATATCGAC TTCGACGTCT GGGACCGGGT GATGACGGTC AGCCTGCGCG GTTATGTGGC CGCCATGAAA TATGCAATCC CGCGCATGCT GGACCGCGGC GGAGGGGCCA TCGTCAACAT GTCATCGGCC GCGGCGTTTC AGGGTGAGCC CGCCCGGCCG GCCTATGCCA CCGCGAAAGC CGGCATCGGC GCTCTCACGC GCCACGTCGC GTCGCGGTGG GGCAAGGACA ACATCCGGTG CAACGCGGTC GCGCCCGGGT TCACCGCCAC CGAGACCATC CGGTCGGTGC CGCAATGGCC GGAGCTGGAA GCCGCCGCGC TCAAGCGAAT TCGTGGGCCG CGCGTCGGCG ACCCGGCCGA CGTCGCCACG TTGGTCGCGT TTCTGCTCTC CGCCGAGGGT GATTGGATCA ACGGTCAGGT CATCAACATC GACGGCGGCA CCGTCCTTCG G

Details for MypaA.00414.a.A1.GE29446

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	29kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	55

Validated AA Sequence

MXLXGLADKV AVXVGGXTGI AAAGGTATQV AFDLADPASV ATLIDCAATT YGGVDLLFNV GADMSTIRAD TDVVDIXFDV WDRVMTVSLR GXVAAMKYAI PRMLDRGGGA IVNMSSAAAF QGEPARPAYA TAKAGIGALT RHVASRWGKD NIRCNAVAPG F

Validated NT Sequence

ggtgaacccg ggcgcgaccg cgttgcaccg gatgttgtcc ttgccccacc gcgacgcgac gtggcgcgtg agagcgccga tgccggcttt cgcggtggca taggccggcc gggcgggctc accctgaaac gccgcggccg atgacatgtt gacgatggcc cctccgccgc ggtccagcat gcgcgggatt gcatatttca tggcggccac ntaaccgcgc aggctgaccg tcatcacccg gtcccagacg tcgaantcga tatcgaccac gtcggtgtcg gcccggatgg tgctcatgtc ggcgcccacg ttgaacagca ggtccacgcc gccgtaagtc gttgcggcac agtcgatcag ggtggcaacc gacgccgggt cggcgaggtc gaaggccacc tgggtcgccg tgccgccggc ggcggcgatg ccggtggncc cnccgaccan nacagcgacc ttgtccgcca gaccgngaag anccatggaa atccccttca ccgc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLRRVAAVK GISMGLRGLA DKVAVVVGGA TGIGAATAAR LAGEGCRVVI GDVAVDAARQ TADRIAAAGG TATQVAFDLA DPASVATLID CAATTYGGVD LLFNVGADMS TIRADTDVVD IDFDVWDRVM TVSLRGYVAA MKYAIPRMLD RGGGAIVNMS SAAAFQGEPA RPAYATAKAG IGALTRHVAS RWGKDNIRCN AVAPGFTATE TIRSVPQWPE LEAAALKRIR GPRVGDPADV ATLVAFLLSA EGDWINGQVI NIDGGTVLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcggcgg gttgcggcgg tgaaggggat ttccatgggt cttcgcggtc tggcggacaa ggtcgctgtc gtggtcggcg gggccaccgg catcggtgcg gcgacggccg cccggctggc cggggaaggc tgccgcgtgg tcatcggcga cgtggccgtc gacgccgcgc ggcagacggc cgaccgcatc gccgccgccg gcggcacggc gacccaggtg gccttcgacc tcgccgaccc ggcgtcggtt gccaccctga tcgactgtgc cgcaacgact tacggcggcg tggacctgct gttcaacgtg ggcgccgaca tgagcaccat ccgggccgac accgacgtgg tcgatatcga cttcgacgtc tgggaccggg tgatgacggt cagcctgcgc ggttatgtgg ccgccatgaa atatgcaatc ccgcgcatgc tggaccgcgg cggaggggcc atcgtcaaca tgtcatcggc cgcggcgttt cagggtgagc ccgcccggcc ggcctatgcc accgcgaaag ccggcatcgg cgctctcacg cgccacgtcg cgtcgcggtg gggcaaggac aacatccggt gcaacgcggt cgcgcccggg ttcaccgcca ccgagaccat ccggtcggtg ccgcaatggc cggagctgga agccgccgcg ctcaagcgaa ttcgtgggcc gcgcgtcggc gacccggccg acgtcgccac gttggtcgcg tttctgctct ccgccgaggg tgattggatc aacggtcagg tcatcaacat cgacggcggc accgtccttc ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MypaA.00414.a.A1.PS00729

PURIFICATION DATe:	8/24/2010
CONCENTRATION:	33.39mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	96
PERCENT COVERAGE:	55

Protocol Notes

notes unavailable

Validated AA Sequence

MXLXGLADKV AVXVGGXTGI AAAGGTATQV AFDLADPASV ATLIDCAATT YGGVDLLFNV GADMSTIRAD TDVVDIXFDV WDRVMTVSLR GXVAAMKYAI PRMLDRGGGA IVNMSSAAAF QGEPARPAYA TAKAGIGALT RHVASRWGKD NIRCNAVAPG F

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCGGCGG GTTGCGGCGG TGAAGGGGAT TTCCATGGGT CTTCGCGGTC TGGCGGACAA GGTCGCTGTC GTGGTCGGCG GGGCCACCGG CATCGGTGCG GCGACGGCCG CCCGGCTGGC CGGGGAAGGC TGCCGCGTGG TCATCGGCGA CGTGGCCGTC GACGCCGCGC GGCAGACGGC CGACCGCATC GCCGCCGCCG GCGGCACGGC GACCCAGGTG GCCTTCGACC TCGCCGACCC GGCGTCGGTT GCCACCCTGA TCGACTGTGC CGCAACGACT TACGGCGGCG TGGACCTGCT GTTCAACGTG GGCGCCGACA TGAGCACCAT CCGGGCCGAC ACCGACGTGG TCGATATCGA CTTCGACGTC TGGGACCGGG TGATGACGGT CAGCCTGCGC GGTTATGTGG CCGCCATGAA ATATGCAATC CCGCGCATGC TGGACCGCGG CGGAGGGGCC ATCGTCAACA TGTCATCGGC CGCGGCGTTT CAGGGTGAGC CCGCCCGGCC GGCCTATGCC ACCGCGAAAG CCGGCATCGG CGCTCTCACG CGCCACGTCG CGTCGCGGTG GGGCAAGGAC AACATCCGGT GCAACGCGGT CGCGCCCGGG TTCACCGCCA CCGAGACCAT CCGGTCGGTG CCGCAATGGC CGGAGCTGGA AGCCGCCGCG CTCAAGCGAA TTCGTGGGCC GCGCGTCGGC GACCCGGCCG ACGTCGCCAC GTTGGTCGCG TTTCTGCTCT CCGCCGAGGG TGATTGGATC AACGGTCAGG TCATCAACAT CGACGGCGGC ACCGTCCTTC GGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA