MyabA.00414.a

Probable short-chain dehydrogenase/reductase

CENTER ID: MyabA.00414.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00414.a.A1.GE30299 Full length( MyabA.00414.a ) 1 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00414.a.A1.PW30176 Full length( MyabA.00414.a ) 1 250

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1258
RefSeq: YP_001701961.1
UniProt: B1MKL5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGEFDNKVAI VTGAAQGIGE AYAHALAREG AAVVVADINA EMGEGVAKQI VADGGRAIFA GVDVSDPDSA IAMADKAVSE FGGIDYLVNN AAIYGGMKLD LLLSVPWDYY KKFMSVNQDG ALVCTRAVYK HIAQRGGGAI VNQSSTAAWL YSGFYGLAKV GINGLTQQLS RELGGQKIRI NAIAPGPTDT EATRGTVPEQ FRSELVKNIP LSRMGTVDDM VGMCLFLLSD KASWITGQVF NVDGGQIIRS
NT Sequence
ATGGGTGAGT TTGATAACAA GGTCGCCATT GTCACCGGCG CCGCACAGGG CATCGGCGAG GCATACGCGC ACGCGCTGGC CCGCGAGGGC GCCGCGGTGG TGGTCGCCGA TATCAATGCC GAGATGGGTG AGGGCGTCGC CAAGCAGATC GTCGCCGACG GCGGTAGGGC CATCTTCGCA GGTGTGGACG TCTCCGATCC CGATTCGGCG ATCGCCATGG CAGACAAGGC TGTCAGCGAG TTCGGCGGCA TCGACTATCT GGTGAACAAC GCCGCCATCT ACGGCGGTAT GAAGCTCGAC CTGCTGCTCT CGGTGCCCTG GGATTACTAC AAGAAATTCA TGAGCGTGAA CCAGGACGGG GCGCTGGTGT GCACCCGCGC GGTGTACAAG CACATCGCTC AGCGCGGCGG CGGCGCCATT GTCAATCAGT CGTCCACCGC CGCGTGGCTG TACTCCGGCT TCTACGGATT GGCCAAGGTT GGCATCAACG GCCTGACGCA GCAGTTGTCC CGAGAGCTGG GCGGGCAGAA GATTCGCATC AACGCCATCG CGCCGGGGCC GACCGACACC GAGGCCACCC GCGGCACCGT GCCCGAGCAA TTCCGCAGCG AGTTGGTCAA GAACATTCCG CTGAGCCGCA TGGGAACTGT TGACGACATG GTGGGCATGT GTCTTTTCCT GTTGTCGGAT AAGGCGTCGT GGATCACCGG CCAGGTATTC AACGTCGATG GCGGACAGAT CATCCGGTCA
Details for MyabA.00414.a.A1.GE30299
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGEFDNKVA IVTGAAQGIG EAYAHALARE GAAVVVADIN AEMGEGVAKQ IVADGGRAIF AGVDVSDPDS AIAMADKAVS EFGGIDYLVN NAAIYGGMKL DLLLSVPWDY YKKFMSVNQD GALVCTRAVY KHIAQRGGGA IVNQSSTAAW LYSGFYGLAK VGINGLTQQL SRELGGQKIR INAIAPGPTD TEATRGTVPE QFRSELVKNI PLSRMGTVDD MVGMCLFLLS DKASWITGQV FNVDGGQIIR S
Validated NT Sequence
ttattatgac cggatgatct gtccgccatc gacgttgaat acctggccgg tgatccacga cgccttatcc gacaacagga aaagacacat gcccaccatg tcgtcaacag ttcccatgcg gctcagcgga atgttcttga ccaactcgct gcggaattgc tcgggcacgg tgccgcgggt ggcctcggtg tcggtcggcc ccggcgcgat ggcgttgatg cgaatcttct gcccgcccag ctctcgggac aactgctgcg tcaggccgtt gatgccaacc ttggccaatc cgtagaagcc ggagtacagc cacgcggcgg tggacgactg attgacaatg gcgccgccgc cgcgctgagc gatgtgcttg tacaccgcgc gggtgcacac cagcgccccg tcctggttca cgctcatgaa tttcttgtag taatcccagg gcaccgagag cagcaggtcg agcttcatac cgccgtagat ggcggcgttg ttcaccagat agtcgatgcc gccgaactcg ctgacagcct tgtctgccat ggcgatcgcc gaatcgggat cggagacgtc cacacctgcg aagatggccc taccgccgtc ggcgacgatc tgcttggcga cgccctcacc catctcggca ttgatatcgg cgaccaccac cgcggcgccc tcgcgggcca gcgcgtgcgc gtatgcctcg ccgatgccct gtgcggcgcc ggtgacaatg gcgaccttgt tatcaaactc acccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGEFDNKVA IVTGAAQGIG EAYAHALARE GAAVVVADIN AEMGEGVAKQ IVADGGRAIF AGVDVSDPDS AIAMADKAVS EFGGIDYLVN NAAIYGGMKL DLLLSVPWDY YKKFMSVNQD GALVCTRAVY KHIAQRGGGA IVNQSSTAAW LYSGFYGLAK VGINGLTQQL SRELGGQKIR INAIAPGPTD TEATRGTVPE QFRSELVKNI PLSRMGTVDD MVGMCLFLLS DKASWITGQV FNVDGGQIIR S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgagttt gataacaagg tcgccattgt caccggcgcc gcacagggca tcggcgaggc atacgcgcac gcgctggccc gcgagggcgc cgcggtggtg gtcgccgata tcaatgccga gatgggtgag ggcgtcgcca agcagatcgt cgccgacggc ggtagggcca tcttcgcagg tgtggacgtc tccgatcccg attcggcgat cgccatggca gacaaggctg tcagcgagtt cggcggcatc gactatctgg tgaacaacgc cgccatctac ggcggtatga agctcgacct gctgctctcg gtgccctggg attactacaa gaaattcatg agcgtgaacc aggacggggc gctggtgtgc acccgcgcgg tgtacaagca catcgctcag cgcggcggcg gcgccattgt caatcagtcg tccaccgccg cgtggctgta ctccggcttc tacggattgg ccaaggttgg catcaacggc ctgacgcagc agttgtcccg agagctgggc gggcagaaga ttcgcatcaa cgccatcgcg ccggggccga ccgacaccga ggccacccgc ggcaccgtgc ccgagcaatt ccgcagcgag ttggtcaaga acattccgct gagccgcatg ggaactgttg acgacatggt gggcatgtgt cttttcctgt tgtcggataa ggcgtcgtgg atcaccggcc aggtattcaa cgtcgatggc ggacagatca tccggtcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyabA.00414.a.A1.PW30176
PURIFICATION DATe: 1/3/2011
CONCENTRATION: 28.73mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 26
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGEFDNKVA IVTGAAQGIG EAYAHALARE GAAVVVADIN AEMGEGVAKQ IVADGGRAIF AGVDVSDPDS AIAMADKAVS EFGGIDYLVN NAAIYGGMKL DLLLSVPWDY YKKFMSVNQD GALVCTRAVY KHIAQRGGGA IVNQSSTAAW LYSGFYGLAK VGINGLTQQL SRELGGQKIR INAIAPGPTD TEATRGTVPE QFRSELVKNI PLSRMGTVDD MVGMCLFLLS DKASWITGQV FNVDGGQIIR S
Validated NT Sequence
ttattatgac cggatgatct gtccgccatc gacgttgaat acctggccgg tgatccacga cgccttatcc gacaacagga aaagacacat gcccaccatg tcgtcaacag ttcccatgcg gctcagcgga atgttcttga ccaactcgct gcggaattgc tcgggcacgg tgccgcgggt ggcctcggtg tcggtcggcc ccggcgcgat ggcgttgatg cgaatcttct gcccgcccag ctctcgggac aactgctgcg tcaggccgtt gatgccaacc ttggccaatc cgtagaagcc ggagtacagc cacgcggcgg tggacgactg attgacaatg gcgccgccgc cgcgctgagc gatgtgcttg tacaccgcgc gggtgcacac cagcgccccg tcctggttca cgctcatgaa tttcttgtag taatcccagg gcaccgagag cagcaggtcg agcttcatac cgccgtagat ggcggcgttg ttcaccagat agtcgatgcc gccgaactcg ctgacagcct tgtctgccat ggcgatcgcc gaatcgggat cggagacgtc cacacctgcg aagatggccc taccgccgtc ggcgacgatc tgcttggcga cgccctcacc catctcggca ttgatatcgg cgaccaccac cgcggcgccc tcgcgggcca gcgcgtgcgc gtatgcctcg ccgatgccct gtgcggcgcc ggtgacaatg gcgaccttgt tatcaaactc acccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGEFDNKVA IVTGAAQGIG EAYAHALARE GAAVVVADIN AEMGEGVAKQ IVADGGRAIF AGVDVSDPDS AIAMADKAVS EFGGIDYLVN NAAIYGGMKL DLLLSVPWDY YKKFMSVNQD GALVCTRAVY KHIAQRGGGA IVNQSSTAAW LYSGFYGLAK VGINGLTQQL SRELGGQKIR INAIAPGPTD TEATRGTVPE QFRSELVKNI PLSRMGTVDD MVGMCLFLLS DKASWITGQV FNVDGGQIIR S
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTGAGTTT GATAACAAGG TCGCCATTGT CACCGGCGCC GCACAGGGCA TCGGCGAGGC ATACGCGCAC GCGCTGGCCC GCGAGGGCGC CGCGGTGGTG GTCGCCGATA TCAATGCCGA GATGGGTGAG GGCGTCGCCA AGCAGATCGT CGCCGACGGC GGTAGGGCCA TCTTCGCAGG TGTGGACGTC TCCGATCCCG ATTCGGCGAT CGCCATGGCA GACAAGGCTG TCAGCGAGTT CGGCGGCATC GACTATCTGG TGAACAACGC CGCCATCTAC GGCGGTATGA AGCTCGACCT GCTGCTCTCG GTGCCCTGGG ATTACTACAA GAAATTCATG AGCGTGAACC AGGACGGGGC GCTGGTGTGC ACCCGCGCGG TGTACAAGCA CATCGCTCAG CGCGGCGGCG GCGCCATTGT CAATCAGTCG TCCACCGCCG CGTGGCTGTA CTCCGGCTTC TACGGATTGG CCAAGGTTGG CATCAACGGC CTGACGCAGC AGTTGTCCCG AGAGCTGGGC GGGCAGAAGA TTCGCATCAA CGCCATCGCG CCGGGGCCGA CCGACACCGA GGCCACCCGC GGCACCGTGC CCGAGCAATT CCGCAGCGAG TTGGTCAAGA ACATTCCGCT GAGCCGCATG GGAACTGTTG ACGACATGGT GGGCATGTGT CTTTTCCTGT TGTCGGATAA GGCGTCGTGG ATCACCGGCC AGGTATTCAA CGTCGATGGC GGACAGATCA TCCGGTCATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A